Use of restriction enzymes

[Dr. Laila Nimri]

Dear colleagues,
I am using the restriction enzyme (Hae III, from Promega) to digest a PCR product
of  Leishmania DNA for the purpose of characterization of these spp. I did the
digest several times according to the manufacturer's instructions with no luck. I
also increased the enzyme concentration but it didn't work, I only got a band of
the undigested DNA. I would really appreciate it if anyone who used this
restriction enzyme can provide me with the right conditions to use. It would be
nice if I could also have the optimum conditions of other restriction enzymes that
worked on Leishmania.
Laila Nimri

Jeffrey Shaw wrote:

> Dear Colleagues,
> As some of you know, I am constructing a comprehensive database of sand
> flies in colony throughout the world. So far the response has been very
> good and I now have 56 colonies of 27 species registered in the database.
> Many thanks to those who have contributed so far. Now I would like to
> broaden the search for other existing colonies, particularly those in
> Europe. If my memory serves me correctly, there was an address list (e-mail
> list) generated at the ISOPS III in Montpelier of all attendees. If any of
> you can help me obtain a copy of that list it will be greatly appreciated.
> I'm sure Micheli Maroli knows of some colonies in Italy, but I don't have
> his e-mail address either. Do you?
> I ask for your help in disseminating this request for information about
> existing sand fly colonies? If you know of other workers who have colonies,
> please forward this message to them or send me their e-mail or mailing
> addresses and I will contact them directly.
> The essential elements of information needed on each colony are:
> 1. Name of Laboratory
> 2. Investigator Name(s)
> 3. Complete mailing address(es) of investigator(s)
> 4. Phone number (include country and city codes)
> 5. Fax number (include country and city codes)
> 6. E-mail address(es) of investigator(s)
> 7. Species in colony (list all strains of each species in colony)
> 8. Site of origin (for each species and strain)
> 9. Approximate number of generations in colony
> 10. Larval diet used
> 11. Adult diet used (human, hamster, rabbit, membrane, etc.;type of sugar
> meal)
> 12. Rearing temperature and relative humidity
> 13. Light Dark Cycle (12light/12dark; 16 light/8 dark)
> 14. Associated references (publications about the colony or publications
> resulting from experiments conducted using colony material)
> This database is intended to be a resource for all sand fly workers and will
> be made available on the web. It will be updated from time to time as more
> information is received.
> Thank you sincerely,
> Phillip G. Lawyer, Ph.D.
> Uniformed Services University of the Health Sciences
> 4301 Jones Bridge Road
> Bethesda, MD 20814, USA
> Phone: (301) 295-3735
> Fax: (301) 295-3860
> E-mail: <plawyer at usuhs.mil>
>
> --MS_Mac_OE_3067232602_15238671_MIME_Part
> Content-type: text/html; charset="US-ASCII"
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> Dear Colleagues,
>
> As some of you know, I am constructing a comprehensive database of sand
> fli= es in colony throughout the world. So far the response has been very
> g= ood and I now have 56 colonies of 27 species registered in the database.
> &nb= sp;Many thanks to those who have contributed so far. Now I would like
> = to broaden the search for other existing colonies, particularly those in
> Eur= ope. If my memory serves me correctly, there was an address list
> (e-ma= il list) generated at the ISOPS III in Montpelier of all attendees.
> If= any of you can help me obtain a copy of that list it will be greatly
> apprec= iated. I'm sure Micheli Maroli knows of some colonies in Italy, but
> I don't = have his e-mail address either. Do you?
>
> I ask for your help in disseminating this request for information about
> exi= sting sand fly colonies? If you know of other workers who have
> colonie= s, please forward this message to them or send me their e-mail or
> mailing ad= dresses and I will contact them directly.
>
> The essential elements of information needed on each colony are:
>
> 1. Name of Laboratory
> 2. Investigator Name(s)
> 3. Complete mailing address(es) of investigator(s)
> 4. Phone number (include country and city codes)
> 5. Fax number (include country and city codes)
> 6. E-mail address(es) of investigator(s)
> 7. Species in colony (list all strains of each species in colony)
> 8. Site of origin (for each species and strain)
> 9. Approximate number of generations in colony
> 10. Larval diet used
> 11. Adult diet used (human, hamster, rabbit, membrane, etc.;type of s= ugar
> meal)
> 12. Rearing temperature and relative humidity
> 13. Light Dark Cycle (12light/12dark; 16 light/8 dark)
> 14. Associated references (publications about the colony or publicati= ons
> resulting from experiments conducted using colony material)
>
> This database is intended to be a resource for all sand fly workers and wil= l
> be made available on the web. It will be updated from time to time as= more
> information is received.
>
> Thank you sincerely,
>
> Phillip G. Lawyer, Ph.D.
> Uniformed Services University of the Health Sciences
> 4301 Jones Bridge Road
> Bethesda, MD 20814, USA
>
> Phone: (301) 295-3735
> Fax: (301) 295-3860
> E-mail: <plawyer at usuhs.mil> = ;
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