looks like amastigotes

Thu Mar 18 15:40:46 BRT 1999

Parasites are highly sugestive of Toxoplasma. Why don't you try to isolate
the parasite? Bone marrow aspirate could be inoculated intraperitoneally in
mice. The peritonaeal fluid becomes positive in 6 to 10 days. This
procedure should be done as soon as possible or specimens stored overnight
at 4oC until inoculation. It should not be frozen or placed in formalin
because it kills the parasites. It is also important to know the patient's
sorological condition for HIV. 
Marise.

At 13:19 18/03/99 -0300, you wrote:
>They are bigger than amastigotes of L.donovani, if the ghosts of
>erythrocytes are used for comparision, They had a fusiform appearence,
>without clear kynetoplast. What are the patient simptons and signs?
>Toxoplasma gondii must be considered, but the form are not clearly curve, as
>most tachyzoites appear in smears.
>Heitor
>-----Mensagem original-----
>De: mk <mkorkmaz at med.ege.edu.tr>
>Para: Multiple recipients of list <leish-l at bdt.org.br>
>Data: Wednesday, March 17, 1999 1:42 PM
>Assunto: looks like amastigotes
>
>
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>>Dear all;
>>I will show you a photograph of bone marrow puncture slide stained by =
>>Giemsa at (http://med.ege.edu.tr/~mkorkmaz/suspect.htm ). The patient =
>>suspected as kala-azar and it was sended for searching Leishmania =
>>amastigotes. There was no amastigotes? at the slide.  Leishmaniasis =
>>serology (IFA,ELISA) negative.=20
>>But I saw a different organism and showed it to microbiology and =
>>pathology department. They could not give an idea about it. When I asked =
>>could be Histoplasma capsulatum? They say no. Please help me if any one =
>>knows this organism. Thank you in advance.
>>
>>Seray Ozensoy, MD
>>
>>
>>------=_NextPart_000_0036_01BE70A4.15508AE0
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>><!DOCTYPE HTML PUBLIC "-//W3C//DTD W3 HTML//EN">
>><HTML><HEAD>
>><META content=3D"text/html; charset=3Dwindows-1254" =
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>><META content=3D'"MSHTML 5.00.0910.1309"' name=3DGENERATOR></HEAD>
>><BODY bgColor=3D#ffffff>
>><DIV><FONT face=3DArial size=3D2>Dear all;</FONT></DIV>
>><DIV><FONT face=3DArial size=3D2>I will show you a photograph of bone =
>>marrow=20
>>puncture slide stained by Giemsa at (<A=20
>>href=3D"http://med.ege.edu.tr/~mkorkmaz/suspect.htm">http://med.ege.edu.t=
>>r/~mkorkmaz/suspect.htm</A>=20
>>). The patient suspected as kala-azar and it was sended for searching =
>>Leishmania=20
>>amastigotes. There was no amastigotes? at the slide.  Leishmaniasis =
>>serology=20
>>(IFA,ELISA) negative. </FONT></DIV>
>><DIV><FONT face=3DArial size=3D2>But I saw a different organism and =
>>showed it to=20
>>microbiology and pathology department. They could not give an idea about =
>>it.=20
>>When I asked could be Histoplasma capsulatum? They say no. Please help =
>>me if any=20
>>one knows this organism. Thank you in advance.</FONT></DIV>
>><DIV>&nbsp;</DIV>
>><DIV><FONT face=3DArial size=3D2>Seray Ozensoy, MD</FONT></DIV>
>><DIV>&nbsp;</DIV></BODY></HTML>
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