looks like amastigotes

Fri Mar 19 16:13:47 BRT 1999

It is easy to exclude Histoplasma if you still have any doubt. Just make a
silver stain (Grocott). Although  they don't look like because of their
shape and size.
Marise Mattos

At 14:52 18/03/99 -0300, you wrote:
>Parasites are highly sugestive of Toxoplasma. Why don't you try to isolate
>the parasite? Bone marrow aspirate could be inoculated intraperitoneally in
>mice. The peritonaeal fluid becomes positive in 6 to 10 days. This
>procedure should be done as soon as possible or specimens stored overnight
>at 4oC until inoculation. It should not be frozen or placed in formalin
>because it kills the parasites. It is also important to know the patient's
>sorological condition for HIV. 
>Marise.
>
>
>At 13:19 18/03/99 -0300, you wrote:
>>They are bigger than amastigotes of L.donovani, if the ghosts of
>>erythrocytes are used for comparision, They had a fusiform appearence,
>>without clear kynetoplast. What are the patient simptons and signs?
>>Toxoplasma gondii must be considered, but the form are not clearly curve, as
>>most tachyzoites appear in smears.
>>Heitor
>>-----Mensagem original-----
>>De: mk <mkorkmaz at med.ege.edu.tr>
>>Para: Multiple recipients of list <leish-l at bdt.org.br>
>>Data: Wednesday, March 17, 1999 1:42 PM
>>Assunto: looks like amastigotes
>>
>>
>>>This is a multi-part message in MIME format.
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>>>------=_NextPart_000_0036_01BE70A4.15508AE0
>>>Content-Type: text/plain;
>>> charset="iso-8859-9"
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>>>Dear all;
>>>I will show you a photograph of bone marrow puncture slide stained by =
>>>Giemsa at (http://med.ege.edu.tr/~mkorkmaz/suspect.htm ). The patient =
>>>suspected as kala-azar and it was sended for searching Leishmania =
>>>amastigotes. There was no amastigotes? at the slide.  Leishmaniasis =
>>>serology (IFA,ELISA) negative.=20
>>>But I saw a different organism and showed it to microbiology and =
>>>pathology department. They could not give an idea about it. When I asked =
>>>could be Histoplasma capsulatum? They say no. Please help me if any one =
>>>knows this organism. Thank you in advance.
>>>
>>>Seray Ozensoy, MD
>>>
>>>
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>>><!DOCTYPE HTML PUBLIC "-//W3C//DTD W3 HTML//EN">
>>><HTML><HEAD>
>>><META content=3D"text/html; charset=3Dwindows-1254" =
>>>http-equiv=3DContent-Type>
>>><META content=3D'"MSHTML 5.00.0910.1309"' name=3DGENERATOR></HEAD>
>>><BODY bgColor=3D#ffffff>
>>><DIV><FONT face=3DArial size=3D2>Dear all;</FONT></DIV>
>>><DIV><FONT face=3DArial size=3D2>I will show you a photograph of bone =
>>>marrow=20
>>>puncture slide stained by Giemsa at (<A=20
>>>href=3D"http://med.ege.edu.tr/~mkorkmaz/suspect.htm">http://med.ege.edu.t=
>>>r/~mkorkmaz/suspect.htm</A>=20
>>>). The patient suspected as kala-azar and it was sended for searching =
>>>Leishmania=20
>>>amastigotes. There was no amastigotes? at the slide.  Leishmaniasis =
>>>serology=20
>>>(IFA,ELISA) negative. </FONT></DIV>
>>><DIV><FONT face=3DArial size=3D2>But I saw a different organism and =
>>>showed it to=20
>>>microbiology and pathology department. They could not give an idea about =
>>>it.=20
>>>When I asked could be Histoplasma capsulatum? They say no. Please help =
>>>me if any=20
>>>one knows this organism. Thank you in advance.</FONT></DIV>
>>><DIV>&nbsp;</DIV>
>>><DIV><FONT face=3DArial size=3D2>Seray Ozensoy, MD</FONT></DIV>
>>><DIV>&nbsp;</DIV></BODY></HTML>
>>>
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>>
>>
>>
>
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