L.infantum?

[Douglas Barker]

>From Douglas Barker and Bronwen Lambson.
Re your pregnant wowan case.
We have a DNA probe called EH which is specific for L. infantum/L chagasi .
We also have another probe B4 RSA  which recongnises L. donovani
minicircles. If you can send us a small ammount of your PCR product we can
reamplify and do a couple of southerns with our probes. THIS SHOULD CLARIFY
WHICH OF THE SPECIES YOUR DNA PCR PRODUCT COMES FROM. WE ALSO HAVE A LARGE
NUMBER OF DNA SEQUENCES SOME OF WHICH ARE IN THE DATA BANK AND SOME NOT
FROM VARIOUS L. DONOVANI AND L. INFANTUM MINICIRCLES. WE CAN LET YOU
COMPARE THESE SEQUENCES IF YOU WISH.





We report a case of a pregnant woman infected with a parasite that caused
>abortion. Histological studies revealed protozoan parasites in placenta
>and in fetal rests, we can determine thad was a Kinetoplastida by light
>and electron microscopy. In order to ensure this point we have extracted
>the total DNA from paraffin embedded tissue and we have obtained by PCR
>the minicircle DNA sequence. When we compare this sequence with the sequences
>in the GenBank data base the best score was obtained with L.infantum
>(50%). My question is: We can establish that the infection was due to
>Leishmania infantum?
>
>Thank you very much