Blood meals from sand flies
Climent Bordier
bordier1 at iprolink.ch
Tue Mar 19 07:46:09 BRT 1996
> Does anybody know of the existence of a kit to determine the blood
> meal of sand flies using ELISA?
>
> Thank you
>
> jorge at opas.org.br
>
We have produced a few years ago a prototype kit for the analysis of blood
meal in phlebotominae.
The method was based on the identification of the Ig's species in the
sample. The steps were as follows:
1) Spot anti-Ig for different species on nitrocellulose.
2) Add diluted blood meal sample (previously stored dry on paper).
3) Add anti_Ig (anti-species) conjugated to peroxidase.
4) Reveal PO activity with Chloronaphthol. A positive sample shows up as a
blue dot.
As manufacturer, I found that the production of this kit was very tedious!!
Recently De Colmenares et al. (J. Med. Entomol. 32, 229-233, 1995) described
an other method which looks convenient:
1) Sensitize an ELISA plate with anti-Ig for different species.
2) Add a mixture of the blood sample and a limiting amount of biotinylated
anti-Ig.
3) Add avidine-peroxidase conjugate.
4) Add PO Substrates (TMB).
This is a competitive assay and a positive sample shows a reduced color
development.
Sincerely yours,
Clement Bordier, Bordier Affinity Products,
Biokema building, Chatanerie 2, CH-1023 Crissier, Switzerland
Phone: +4121 / 634 2845, Fax: +4121 / 635 2064. bordier1 at iprolink.ch
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