[Leish-l] Fwd: Growth curve in promastigote culture

[Carlos Costa]

Gabriela,

Ninguém melhor do que você para responder a esta jovem pesquisadora.

Abraços,

Carlos.

---------- Forwarded message ---------
From: Nayore Tk <nayoretk at gmail.com>
Date: Mon, Aug 14, 2023 at 12:40 PM
Subject: [Leish-l] Growth curve in promastigote culture
To: <leish-l at lineu.icb.usp.br>


Good afternoon,

My name is Nayore, I am a doctoral student and researcher at UFSCar and I
work with Leishmania infantum promastigotes culture. I have doubts about
how to perform the growth curve, should I do it in 12 well plate or in a
culture flask?

I performed this type of count for other strains, however I was performing
the plate count during 10 days, starting from a concentration of 2.0x10e5
promastigotes/mL in first day. Recently I was asked why I was making it on
a well and not in a flask.

I would like to solve this question about which is the best way to make the
curve in a well containing 2.0x10e5 promastigotes/2 mL or in a culture
flask?

thank you for your attention

Best Regards
Nayore Tamie Takamiya

-- 
*Nayore Tamie Takamiya*
Bióloga/ Doutoranda no Programa de Pós-graduação em Genética Evolutiva e
Biologia Molecular
Universidade Federal de São Carlos (UFSCar)
Centro de Ciências Biológicas e da Saúde (CCBS)
Departamento de Genética e Evolução (DGE)
Rod. Washington Luís, Km 235 Caixa Postal 676
CEP. 13565-905 - São Carlos - SP - Brasil
http://lattes.cnpq.br/6422940683742319
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-- 
Carlos H. N. Costa, MD, DSc.
Head
Instituto de Doenças do Sertão
*Instituto de Doenças Tropicais Natan Portella*

*Centro de Ciências da Saúde da Universidade Federal do Piauí*
*Rua Artur de Vasconcelos 151-Sul*
*64001-450 Teresina-PI*
*Brazil*
Telephones: +55 86 3222-4377 (W),
+55 86 3221-3062 (W),
+55 86 3237-1075 (H).


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