[Leish-l] Status of PCR for CL diagnosis

[Ali Khamesipour]

 We are in Iran using PCR for identification of Leishmania species, PCR is perfect for diagnosis as well but since in most of the endemic areas neither the equipment nor the expert personnel is available, PCR is used for diagnosis of complicated cases.

    On Tuesday, April 17, 2018, 6:21:57 PM GMT+4:30, Aline Fagundes <aline011266 at gmail.com> wrote:  
 
 Olá a todos

Os resultados da Oficina de trabalho resultaram em algum protocolo
padronizado?

Alguma orientação para a coleta, transporte e envio de amostras?

Abraços

Aline Fagundes

Dra Aline Fagundes
Laboratório de Pesquisa Clinica e  Vigilância em Leishmanioses -
LAPClin-Vigileish
Instituto Nacional de Infectologia Evandro Chagas
Fundação Oswaldo Cruz
Rio de Janeiro
Tel/fax: 00-55-21-38659541
email alternativo: aline011266 at gmail.com



2017-06-22 9:19 GMT-03:00 Yadon, Dra. Zaida (AFT) <yadonzai at paho.org>:

> I would like to share with the link to the information publishes by PAHO
> on the  International  initiative for the standardization and validation of
> PCR for CL diagnosis and disease management across laboratories and Latin
> American and Caribbean countries
>
> Web Linlks
>
> Espanol
> http://www.paho.org/hq/index.php?option=com_content&view=
> article&id=13405&Itemid=42367&lang=es
>
>
> English
> http://www.paho.org/hq/index.php?option=com_content&view=
> article&id=13405&Itemid=42367&lang=en
>
>
> Links to PDF:
>
> English
> http://www2.paho.org/hq/index.php?option=com_docman&task=
> doc_view&gid=40483&Itemid=270
>
>
> Español
> http://www2.paho.org/hq/index.php?option=com_docman&task=
> doc_view&gid=40486&Itemid=270
>
>
>
> Portuguese
> http://www2.paho.org/hq/index.php?option=com_docman&task=
> doc_view&gid=40483&Itemid=270
>
>
>
>
>
> Dr. Zaida Yadon
> Regional Advisor on Communicable Diseases Research
> Communicable Diseases and Health Analysis Department
> Pan American Health Organization
> Tel.: + 55 21 3661 9056 - VOIP 49056
> yadonzai at paho.org
>
>
> -----Original Message-----
> From: leish-l-bounces at lineu.icb.usp.br [mailto:leish-l-bounces at lineu.
> icb.usp.br] On Behalf Of Noris Rodriguez
> Sent: Sunday, May 07, 2017 4:36 PM
> To: Kwang-Poo Chang
> Cc: Leish-L
> Subject: Re: [Leish-l] Status of PCR for CL diagnosis
>
> Dear Dr. Valdivia
>
>
>
> In Venezuela, we are using PCR for diagnosis since 1990 and also
> microscopy, but we are doing PCR with species specific oligonucleotides ,
> in order to do both: diagnosis and species identification just in one PCR
> reaction.
>
> I am agree to have a meeting in Toledo, in order to make a regional
> project to evalauate all the PCR protocols  used in each counntry.
>
>
>
> Best Regards
>
>
>
> Dra. Noris Rodriguez
>
> Directora
>
> Instituto de Biomedicina "Dr. Jacinto Convit
>
> Facultad de Medicina
>
> Universidad Central de Venezuela
>
> Telefax: 0212 8648624
>
>
>
>
> 2017-04-12 9:04 GMT-04:30 Kwang-Poo Chang <
> kwangpoo.chang at rosalindfranklin.edu>:
>
> > In sanliurfa, Turkey, dermatologists placed lesion aspirates on glass
> > slides and examined the wet mount preparations for the presence of
> > amastigotes as a quick way of diagnosis. Remaining samples were used
> > for culture and/or PCR, if necessary.
> >
> > This is doable if amastigotes are abundant and large in size.
> >
> > Just pass on the information for your reference.
> >
> > KP
> >
> > On Wed, Apr 5, 2017 at 8:40 AM, Hugo Valdivia <hvalrod at hotmail.com>
> wrote:
> >
> > > Thank you all for your thoughts and insights, it is fantastic to see
> > > all the interaction in the Leish community,
> > >
> > >
> > > I am fully aware of the advantages of PCR and the clear need of
> > > standardization in the region for CL diagnosis. I hope that this
> > > issue
> > will
> > > be discussed on WorldLeish and could result in better diagnostics
> > > for the people who are most affected by this disease.
> > >
> > >
> > > Thank you,
> > >
> > > Hugo Valdivia,
> > >
> > >
> > >
> > >
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