[Leish-l] Status of PCR for CL diagnosis

[Aline Fagundes]

Olá a todos

Os resultados da Oficina de trabalho resultaram em algum protocolo
padronizado?

Alguma orientação para a coleta, transporte e envio de amostras?

Abraços

Aline Fagundes

Dra Aline Fagundes
Laboratório de Pesquisa Clinica e  Vigilância em Leishmanioses -
LAPClin-Vigileish
Instituto Nacional de Infectologia Evandro Chagas
Fundação Oswaldo Cruz
Rio de Janeiro
Tel/fax: 00-55-21-38659541
email alternativo: aline011266 at gmail.com



2017-06-22 9:19 GMT-03:00 Yadon, Dra. Zaida (AFT) <yadonzai at paho.org>:

> I would like to share with the link to the information publishes by PAHO
> on the  International  initiative for the standardization and validation of
> PCR for CL diagnosis and disease management across laboratories and Latin
> American and Caribbean countries
>
> Web Linlks
>
> Espanol
> http://www.paho.org/hq/index.php?option=com_content&view=
> article&id=13405&Itemid=42367&lang=es
>
>
> English
> http://www.paho.org/hq/index.php?option=com_content&view=
> article&id=13405&Itemid=42367&lang=en
>
>
> Links to PDF:
>
> English
> http://www2.paho.org/hq/index.php?option=com_docman&task=
> doc_view&gid=40483&Itemid=270
>
>
> Español
> http://www2.paho.org/hq/index.php?option=com_docman&task=
> doc_view&gid=40486&Itemid=270
>
>
>
> Portuguese
> http://www2.paho.org/hq/index.php?option=com_docman&task=
> doc_view&gid=40483&Itemid=270
>
>
>
>
>
> Dr. Zaida Yadon
> Regional Advisor on Communicable Diseases Research
> Communicable Diseases and Health Analysis Department
> Pan American Health Organization
> Tel.: + 55 21 3661 9056 - VOIP 49056
> yadonzai at paho.org
>
>
> -----Original Message-----
> From: leish-l-bounces at lineu.icb.usp.br [mailto:leish-l-bounces at lineu.
> icb.usp.br] On Behalf Of Noris Rodriguez
> Sent: Sunday, May 07, 2017 4:36 PM
> To: Kwang-Poo Chang
> Cc: Leish-L
> Subject: Re: [Leish-l] Status of PCR for CL diagnosis
>
> Dear Dr. Valdivia
>
>
>
> In Venezuela, we are using PCR for diagnosis since 1990 and also
> microscopy, but we are doing PCR with species specific oligonucleotides ,
> in order to do both: diagnosis and species identification just in one PCR
> reaction.
>
> I am agree to have a meeting in Toledo, in order to make a regional
> project to evalauate all the PCR protocols  used in each counntry.
>
>
>
> Best Regards
>
>
>
> Dra. Noris Rodriguez
>
> Directora
>
> Instituto de Biomedicina "Dr. Jacinto Convit
>
> Facultad de Medicina
>
> Universidad Central de Venezuela
>
> Telefax: 0212 8648624
>
>
>
>
> 2017-04-12 9:04 GMT-04:30 Kwang-Poo Chang <
> kwangpoo.chang at rosalindfranklin.edu>:
>
> > In sanliurfa, Turkey, dermatologists placed lesion aspirates on glass
> > slides and examined the wet mount preparations for the presence of
> > amastigotes as a quick way of diagnosis. Remaining samples were used
> > for culture and/or PCR, if necessary.
> >
> > This is doable if amastigotes are abundant and large in size.
> >
> > Just pass on the information for your reference.
> >
> > KP
> >
> > On Wed, Apr 5, 2017 at 8:40 AM, Hugo Valdivia <hvalrod at hotmail.com>
> wrote:
> >
> > > Thank you all for your thoughts and insights, it is fantastic to see
> > > all the interaction in the Leish community,
> > >
> > >
> > > I am fully aware of the advantages of PCR and the clear need of
> > > standardization in the region for CL diagnosis. I hope that this
> > > issue
> > will
> > > be discussed on WorldLeish and could result in better diagnostics
> > > for the people who are most affected by this disease.
> > >
> > >
> > > Thank you,
> > >
> > > Hugo Valdivia,
> > >
> > >
> > >
> > >
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