[Leish-l] Status of PCR for CL diagnosis

[Thaynan Martins]

Dear Hugo O. Valdivia,

Regarding your question about the use of PCR for CL diagnosis, I'd
like to suggest you to contact Prof. Dr. Paulo Cotrim at the Institute
of Tropical Medicine/ USP.

I was he student few years ago and we work together in the diagnosis
of naturally infected dogs in the great Sao Paulo area. We could
identify and differentiate the three major brazilian clinical relevant
species of leishmania using a single PCR technique targeting a special
sequence in the leishmania kDNA. This project compared the
parasitological methods using 5 different biopsied canine tissues with
two different DNA targets by PCR. I can guarantee you that PCR is more
sensitive, accurate, fast and reliable than microscopy, which require
a very well trained specialist to identify the leishmania on the
"suggestive" slides.

Unfortunately, I had to move to Germany and could not continue the
work with Prof. Cotrim.

Kind regards,

Thaynan Martins

PhD student at the Institute of Infectiology / ZMBE
Zentrum für Molekularbiologie der Entzündung
Von-Esmarch-Straße 56
48149 Münster
Tel.: 0251 83 52101

2017-03-27 6:30 GMT+02:00, Hugo Valdivia <hvalrod at hotmail.com>:
> Dear colleagues,
>
> We have been recently questioned about the use of PCR for CL  diagnosis
> given that the Peruvian Ministry of health considers microscopy as the
> standard practice for CL diagnosis. In this sense, I would like to hear your
> thoughts about the use of PCR for CL diagnosis, specially from those
> colleagues working in reference diagnostic centers. What I reviewed made me
> think that there is no a consensus yet on the topic.
>
> Thank you for the information that you can provide and hope to see you in
> WL6.
>
> Best regards,
> Hugo O. Valdivia,  BSc, PhD
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