[Leish-l] Status of PCR for CL diagnosis

[Yadon, Dra. Zaida (AFT)]

I would like to share with the link to the information publishes by PAHO on the  International  initiative for the standardization and validation of PCR for CL diagnosis and disease management across laboratories and Latin American and Caribbean countries

Web Linlks

Espanol
http://www.paho.org/hq/index.php?option=com_content&view=article&id=13405&Itemid=42367&lang=es


English
http://www.paho.org/hq/index.php?option=com_content&view=article&id=13405&Itemid=42367&lang=en


Links to PDF:

English
http://www2.paho.org/hq/index.php?option=com_docman&task=doc_view&gid=40483&Itemid=270


Español
http://www2.paho.org/hq/index.php?option=com_docman&task=doc_view&gid=40486&Itemid=270



Portuguese
http://www2.paho.org/hq/index.php?option=com_docman&task=doc_view&gid=40483&Itemid=270





Dr. Zaida Yadon
Regional Advisor on Communicable Diseases Research
Communicable Diseases and Health Analysis Department
Pan American Health Organization
Tel.: + 55 21 3661 9056 - VOIP 49056
yadonzai at paho.org


-----Original Message-----
From: leish-l-bounces at lineu.icb.usp.br [mailto:leish-l-bounces at lineu.icb.usp.br] On Behalf Of Noris Rodriguez
Sent: Sunday, May 07, 2017 4:36 PM
To: Kwang-Poo Chang
Cc: Leish-L
Subject: Re: [Leish-l] Status of PCR for CL diagnosis

Dear Dr. Valdivia



In Venezuela, we are using PCR for diagnosis since 1990 and also  microscopy, but we are doing PCR with species specific oligonucleotides , in order to do both: diagnosis and species identification just in one PCR reaction.

I am agree to have a meeting in Toledo, in order to make a regional project to evalauate all the PCR protocols  used in each counntry.



Best Regards



Dra. Noris Rodriguez

Directora

Instituto de Biomedicina "Dr. Jacinto Convit

Facultad de Medicina

Universidad Central de Venezuela

Telefax: 0212 8648624




2017-04-12 9:04 GMT-04:30 Kwang-Poo Chang <
kwangpoo.chang at rosalindfranklin.edu>:

> In sanliurfa, Turkey, dermatologists placed lesion aspirates on glass
> slides and examined the wet mount preparations for the presence of
> amastigotes as a quick way of diagnosis. Remaining samples were used
> for culture and/or PCR, if necessary.
>
> This is doable if amastigotes are abundant and large in size.
>
> Just pass on the information for your reference.
>
> KP
>
> On Wed, Apr 5, 2017 at 8:40 AM, Hugo Valdivia <hvalrod at hotmail.com> wrote:
>
> > Thank you all for your thoughts and insights, it is fantastic to see
> > all the interaction in the Leish community,
> >
> >
> > I am fully aware of the advantages of PCR and the clear need of
> > standardization in the region for CL diagnosis. I hope that this
> > issue
> will
> > be discussed on WorldLeish and could result in better diagnostics
> > for the people who are most affected by this disease.
> >
> >
> > Thank you,
> >
> > Hugo Valdivia,
> >
> >
> >
> >
> > _______________________________________________
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> > Leish-l at lineu.icb.usp.br
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> >
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