[Leish-l] S.O.S.

[Soong, Lynn]

Hi Rita,

We are using the 1500V protocol (Robinson and Beverly, Mol. Biochem Parasitol. 2003) for Lb. It works. 

But, the 450V protocol did not work in our hands. 

Thanks, Lynn 

Lynn Soong, MD, PhD
Professor 
Departments of Microbiology/Immunology and Pathology
Center for Biodefense & Emerging Infectious Diseases
Sealy Center for Vaccine Development
Medical Research Building 3.132
The University of Texas Medical Branch
Galveston, TX 77555-1070
Office phone: 409-772-8149
Lab phone: 409-772-8163
Fax: 409-747-6869
Email: lysoong at utmb.edu

-----Original Message-----
From: leish-l-bounces at lineu.icb.usp.br [mailto:leish-l-bounces at lineu.icb.usp.br] On Behalf Of Rita Mukhopadhyay
Sent: Monday, February 17, 2014 9:41 PM
To: Patrick Bastien; leish-l at lineu.icb.usp.br
Subject: Re: [Leish-l] S.O.S.

Dear Colleagues,

Can anyone please send me transfection protocols for braziliensis and panamensis?  We are trying with G418 selection.  Looks like they are too sensitive to the regular 1500V electroporation protocol.

Thanks a lot for your help.

Rita Mukhopadhyay
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