[Leish-l] why is Leishmania donovani restricted to humans inIndian subcontinent?

[Kwang-Poo Chang]

Dear Ketty,

Thanks a lot for the information !

Could you kindly send the pdf reprints for k26, CBP and hsp90 PCR for
diagnostic discrimination of L donovani complex for clinical samples ? I
assume that they work for dog, sanfly and human samples ?

KP


On Fri, Nov 1, 2013 at 5:34 AM, Σωτηριάδου Καίτη <ksoteriadou at pasteur.gr>wrote:

>
>
> Hi! In our hands also 13A/13B kDNA had the same problems. We came to the
> conclusion that the balance sensitivity/specificity is not good.
> Specifity/contamination is an issue in that sense.******
>
> If you want to discriminate species belonging to the L. donovani complex
>  k26-PCR, CPB and hsp90 give the same results that are not always in
> agreement with MLEE. ITS (among others) is an excellent tool for
> discriminating species causing VL or CL in Middle East. Please note that
> the latter tools can be applied on clinical samples which is an important
> issue. I could sent you the relevant papers in a pdf format or the
> citation, there are several. Please let me know.****
>
> Ketty Soteriadou (Greece)****
>
>
>
> The one by Reale is the 13A/13B kDNA protocol. Its extremely sensitive.
> We've tried it. The problem is that after sometime, one start to see that
> its becoming positive for all samples. Then we gave it up for the benefit
> of genomic-based methods. Perhaps we should have used UNG-uracil in the PCR
> setup as an anti-contamination agent.
>
> Amer
> Palestine
>
>
>
>
>   ------------------------------
> *From:* Stefano Reale <stefano.reale at izssicilia.it>
> *To:* Kwang-Poo Chang <kwangpoo.chang at rosalindfranklin.edu>
> *Cc:* "Lawyer, Phillip (NIH/NIAID) [E]" <PhillipL at niaid.nih.gov>; "
> leish-l-bounces at lineu.icb.usp.br" <leish-l-bounces at lineu.icb.usp.br>;
> Rossella Colomba Lelli <rossella.lelli at izssicilia.it>; Leish-L <
> Leish-L at lineu.icb.usp.br>; "caporalevincenzo at gmail.com" <
> caporalevincenzo at gmail.com>
> *Sent:* Thursday, October 31, 2013 8:19 AM
> *Subject:* Re: [Leish-l] why is Leishmania donovani restricted to humans
> in Indian subcontinent?
>
>  Hallo Im one of the author of this PCR protocol. I optimised it by
> specific primers and probe for infantum. It work very well and I showed the
> Lod and Loq in variuos biological material taken from leishmaniotic dogs.
> In my lab in Palermo I have a number if collected strains stored in liquid
> N2 ready to create the DNA standard referement serial dilutions fir Real
> time test. I could help who should have these DNA.
> Best regards
> Il giorno 31/ott/2013 00:07, "Kwang-Poo Chang" <
> kwangpoo.chang at rosalindfranklin.edu> ha scritto:
>
> Dear Carlos,
>
> One of my colleagues in Naples, Italy has used primer set for kinetoplast
> minicircle conserved region, Considering ~10,000 copies of minicircvles per
> Leishmania, this is probably the most sensitive PCR, although the primer
> set can also amply all trypanosomes or trypanosomatid protozoa (including
> Leptomonas reported to exist in the Indian kala-azar splenic aspirates).
> Specificity could be an issue in that sense.
>
> KP
>
>
> On Thu, Oct 10, 2013 at 7:31 PM, Carlos Lobo <carloshlobo at gmail.com>wrote:
>
>  Hello guys, good night.
> I'm taking advantage of this opportunity to exchange scientific
> information, much of which I have learned to ask for help.
> I'll start a project on canine leishmaniasis in northeastern Brazil and I need
> to diagnose, by PCR, dogs infected and not infected with Leish.
> I've been doing some analysis of PCR for other experiments, but never did
> for Leish, could someone give me some tips? Like which primer to use?
> Should I collect blood or tissue samples to have more reliability?
> Thank you.
> Carlos Henrique
>
>
> 2013/9/23 Tamrat Abebe Zeleke <tabebezeleke at gmail.com>
>
>  Dear Carlos,
>
>  I agree with the comment given by Phillip Lawyer. The molecular analysis
> of strains or isolates from India, Kenya, and South Western Ethiopia also
> supports this notion. However, the issue of distinct strains in Sudan and
> North Ethiopia opts for the fact that East Africa may be the origin of at
> least the naughty L. donovani strains circulating in the region.
>
>  Tamrat Abebe
> Addis Ababa University College of Health Sciences, School of Medicine ,
> Department of Microbiology, Immunology & Parasitology
> Tikur Anbessa Hospital
> Second floor room number 76
> Tel: +251 911 447227(mobile)
> Email: tamrat.abebe at aau.edu.et
> *
> *
> *
> *
>
>
>
>
> On Mon, Sep 16, 2013 at 4:37 AM, Lawyer, Phillip (NIH/NIAID) [E] <
> PhillipL at niaid.nih.gov> wrote:
>
> Dear Carlos,
>
>
>
> For what it's worth, I believe it most likely happened the other way
> around:  Leishmania donovani was probably introduced to East Africa from
> India during the late 1800s when laborers were brought from India to work
> in the Kenya building the railroad from Mombasa to Uganda and on other
> infrastructure projects.  Leishmania donovani in East Africa is manifest as
> kala azar and is anthroponotic, the same as in India.  The main vector in
> Kenya is Phlebotmus martini, which tends to breed in termite mounds, often
> associated with human dwellings.  Other Symphlebotomus species, Ph.
> vansomerenae and Ph. celiae have also been implicated in L. donovani
> transmission.
>
>
>
> Regards,
>
>
>
> Phil Lawyer
>
> ________________________________
> From: Carlos Brisola Marcondes [cbrisolamarcondes at gmail.com]
> Sent: Friday, August 30, 2013 8:41 AM
> To: Leish-L; leish-l-bounces at lineu.icb.usp.br
> Subject: [Leish-l] why is Leishmania donovani restricted to humans in
> Indian subcontinent?
>
> Dear all,
> Leishmania donovani seems to have been introduced from East Africa to
> Indian subcontinent, where it has infected mostly humans, differently from
> East African foci.
>    Why does this occur? Is this caused by feeding preferences of
> Phlebotomus argentipes, which bites mostly ruminants and humans and is
> associated to houses? Or are dogs rarer in that region than in Brazil,
> where these animals are important reservoirs of Leishmania infantum and
> frequently bitten by Lutzomyia longipalpis?
>
> Sincerely yours
> prof. dr. Carlos Brisola Marcondes
> Dept. Microbiol. Imunol. Parasitol./CCB
> Federal University of Santa Catarina
> Florianópolis (SC)
> CV: http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783901J2
> blog: http://entomomedica.blogspot.com.br/
>
>
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