[leish-l] rK39

[nazar abdalla]

Dear Dr Yusuf
Salam alaikum, here I attached a copy of my message to MSF-Holland for the 
subject of rK39 that you send me lataly. Still I am waiting your idea 
rgarding the last manuscript, keepon writting and send my greeting to all 
Dr. Seray, Dr.Ozbel,Prof.ozgel, Dr. Naevin and the Head dep. and your 
family. bye
Nazar

Date: 30th May 2002.
To: Leish-L at bdt.org.br
Subject: MSF appeals for sera and advice.
Dear technical kala-azar adviser, MSF-Holland,
Our experience with ELISA K39 in Study of Leishmania Reservoirs In Nuba 
Mountains, western Sudan. (1999-2000).
8/1 positive. DAT- blood sera/ ELISA K39 tests in rabbits samples (total 
n=14), as well
3/3 were negatives.
5/1 positive. DAT-spleen smears/ELISA K39 tests in rabbits samples (total 
n=14), as well 6/6 were negatives.
One dog was tested by K39, was found to be positive.
ELISA Dip Stick Test (DST):
ELISA-DST is a modified ELISA test and performed with blood specimens (from 
suspected reservoir)  spotted on filter paper. Samples were cut (circle of 
0.6mm) and kept in 0.9% normal saline (Nacl) overnight. In the next day the 
strip was immersed in the DST-specific solution, after five minutes the 
result was read, if one line was present then it is negative and if two 
lines were present then the sample will be considered as positive.
This test was used in an epidemiological study to screen villagers in an 
area between two hyperendemic foci, visceral and cutaneous leishmaniasis 
(east and west Sudan). The final results was negative in all samples, in 
fact the PCR results of 32 cases were positive and more than 50% of cases 
were positive by LST. Other tools such as DAT, ELISA showed variable 
results. (please have a look to our references).
1-	 Abdalla N.M.,  Eldosh M.A., Osman O.F., Daifalla N.S.,  Magzoub M.M., 
Sero epidemiological Study on Leishmaniasis in The Nuba Mountain - Sudan. 
2000, Acta Parasitologica Turcica, 24 (3): 228-233 .
2- 	Abdalla N.M., Hilmy Z.A., Osman O.F., Daifalla N.S., Magzoub M.M., 
Prolong Serological and Molecular Study on sub-clinical Leishmania focus In 
The  Nuba Mountain, Western Sudan. 50th anniversary of The American Society 
of Trop. Med. And Hy. Nov. 2001.
3-	Abdalla N.M., Ibrahim M.E. , El-Hassan A.M., Osman O.F., Daifalla N.S., 
Barker D.C., Lambson B., Miles M., Mauricio I. , Magzoub M.M., 
Clinico-epidemiological Study On Leishmaniasis In The Nuba Mountain - Sudan. 
2002, Acta Parasitologica Turcica, 26 (1) .



_________________________________________________________________
Chat with friends online, try MSN Messenger: http://messenger.msn.com