L. infantum chagasi genome

Tue Sep 12 12:54:55 BRT 2000

I agree.  We have compared the sequence between numerous genes of L. major
and L. donovani and finda range of 90-100 amino acid identity, depending on
the gene.  Indeed we and others have compared the gene organization of L.
major, T. brucei and T. cruzi and find the same genes in essentially the
same order.  There must be differences among species of Leishmania but they
should be localized by methods other than sequencing before detailed (i.e.
sequence) analysis.  In addition, there are substantial differences among
Leishmania, T. brucei and T cruzi.  This is evident from the numerous
repeated sequence in T. cruzi and the VSG expression site and genes.  It is
also evident from the chromosomes number differences between the species
that is especially evident in T. brucei with its numerous minichromosomes
and fairly small number of larger (>1Mb) chromosomes.
Ken Stuart

-----Original Message-----
From: leish-l at bdt.org.br [mailto:leish-l at bdt.org.br]On Behalf Of Dr
Harry Noyes
Sent: Monday, September 11, 2000 6:01 PM
To: Multiple recipients of list
Subject: RE: L. infantum chagasi genome

L. chagasi is one of the species of Leishmania most closely related to
L. major and is on that ground at least a strange choice for a genome
project. A systematic comparison of the genome organisation of the two
organisms could be done using data from the L. major project. This would
be useful in itself and might  indicate if there are sufficient
differences to warrant a new project.

Harry Noyes
University of Liverpool
School of Biological Sciences
Donnan Laboratory
Crown St.
Liverpool
L69 7ZD
UK

Tel +44 151 794 3627
Fax +44 151 794 3655
email harry at liv.ac.uk
www  http://www.liv.ac.uk/~kempsj/genomics.html

On Mon, 11 Sep 2000, KEN STUART wrote:

>Dear Jeffrey,
>My view is that sequencing the entire genome of another species of
>Leishmania would be unreasonably expensive and not needed.  The completion
>of the L. major genome sequence, its comparison with the genomes of T.
>brucei and T. cruzi along with anticipated DNA microarray studied that
>compare species of Leishmania will identify regions of high similarity and
>differences among Leishmania species.  Such regions would be worth
>sequencing.  The character and consequences of the differences among
>Leishmania species is uncertain at this stage which also makes embarking on
>a full genome sequencing effort unreasonable at this time.
>Regards,
>Ken Stuart
>
>-----Original Message-----
>From: leish-l at bdt.org.br [mailto:leish-l at bdt.org.br]On Behalf Of Jeffrey
>Shaw
>Sent: Tuesday, September 05, 2000 8:31 AM
>To: Multiple recipients of list
>Subject: L. infantum chagasi genome
>
>
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>As you may know there is considerable interest and support in Brazil for 
>genome studies and it has been suggested by some members of the Brazilian 
>scientific community that a candidate parasite for sequencing could be L. 
>(L.)infantum chagasi. Others, however, feel it would be wiser to finish L. 
>(L.)major first and then use this to looking at different regions of the 
>visceral parasite.
>
>Views of subscribers on this would be appreciated
>
>Jeffrey Shaw
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><html>
>As you may know there is considerable interest and support in Brazil for
>genome studies and it has been suggested by some members of the Brazilian
>scientific community that a candidate parasite for sequencing could be
><i>L. (L.)infantum chagasi.</i> Others, however, feel it would be wiser
>to finish <i>L. (L.)major</i> first and then use this to looking at
>different regions of the visceral parasite.<br>
><br>
>Views of subscribers on this would be appreciated<br>
><br>
>Jeffrey Shaw</html>
>
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