[Douglas Barker]

My dear Leishmaniac
If you have rosettes it shows your cultures are doing very well. The only
way to get less rosettes is to subculture more often. But for most of our
purposes we need maximium numbers and rosettes are a good sign that the
cultures are ready for DNA extraction.
>From ye ancient retiring
DR Douglas C Barker  > While working with Leishmania donovanipromastigotes
and amastigotes I
>often encounter the problem of clumpung (rosette formation). I will
>appriciate if anyone can suggest me a remady for the same
>
>**********************************
>Maneesh Jain
>Institute Of Microbial Technology
>Sector 39-A
>Chandigarh
>INDIA.
>***********************************