looks like amastigotes

Tue Apr 6 15:07:05 BRT 1999

I agree with Dr.Pablo. We don't knwow anything else about the suggestions
that were done.
Looking again the photo I remembered a case of disseminated sporothricosis
on an HIV patient. It may be a strong possibility. Are there any cutaneous
lesions?
Dra. Marise Mattos

At 15:54 05/04/99 -0300, you wrote:
>Please share with the leishmaniacs the results of our suggestions:
>
>Namely:
>
>Does the organism seen in the smear, stain with silver (Grocott,
>Silver-methenamine ...)
>
>Patient clinical data and HIV serology
>
>Bone marrow culture results for Leishmania/Trypanosome (NNN and Sneider),
>mice intraperitoneal inoculation and fungal culture (Blood-BHI-agar)
>
>	Commercially availlable monoclonal antibodies can be used for
>inmunoflurescent staining in orther to rule-out Toxoplasma gondii.
>
>Pablo
>
> 13:45 17/03/99 -0300, you wrote:
>>This is a multi-part message in MIME format.
>>
>>------=_NextPart_000_0036_01BE70A4.15508AE0
>>Content-Type: text/plain;
>>	charset="iso-8859-9"
>>Content-Transfer-Encoding: quoted-printable
>>
>>Dear all;
>>I will show you a photograph of bone marrow puncture slide stained by =
>>Giemsa at (http://med.ege.edu.tr/~mkorkmaz/suspect.htm ). The patient =
>>suspected as kala-azar and it was sended for searching Leishmania =
>>amastigotes. There was no amastigotes? at the slide.  Leishmaniasis =
>>serology (IFA,ELISA) negative.=20
>>But I saw a different organism and showed it to microbiology and =
>>pathology department. They could not give an idea about it. When I asked =
>>could be Histoplasma capsulatum? They say no. Please help me if any one =
>>knows this organism. Thank you in advance.
>>
>>Seray Ozensoy, MD
>>
>>
>>------=_NextPart_000_0036_01BE70A4.15508AE0
>>Content-Type: text/html;
>>	charset="iso-8859-9"
>>Content-Transfer-Encoding: quoted-printable
>>
>><!DOCTYPE HTML PUBLIC "-//W3C//DTD W3 HTML//EN">
>><HTML><HEAD>
>><META content=3D"text/html; charset=3Dwindows-1254" =
>>http-equiv=3DContent-Type>
>><META content=3D'"MSHTML 5.00.0910.1309"' name=3DGENERATOR></HEAD>
>><BODY bgColor=3D#ffffff>
>><DIV><FONT face=3DArial size=3D2>Dear all;</FONT></DIV>
>><DIV><FONT face=3DArial size=3D2>I will show you a photograph of bone =
>>marrow=20
>>puncture slide stained by Giemsa at (<A=20
>>href=3D"http://med.ege.edu.tr/~mkorkmaz/suspect.htm">http://med.ege.edu.t=
>>r/~mkorkmaz/suspect.htm</A>=20
>>). The patient suspected as kala-azar and it was sended for searching =
>>Leishmania=20
>>amastigotes. There was no amastigotes? at the slide.  Leishmaniasis =
>>serology=20
>>(IFA,ELISA) negative. </FONT></DIV>
>><DIV><FONT face=3DArial size=3D2>But I saw a different organism and =
>>showed it to=20
>>microbiology and pathology department. They could not give an idea about =
>>it.=20
>>When I asked could be Histoplasma capsulatum? They say no. Please help =
>>me if any=20
>>one knows this organism. Thank you in advance.</FONT></DIV>
>><DIV>&nbsp;</DIV>
>><DIV><FONT face=3DArial size=3D2>Seray Ozensoy, MD</FONT></DIV>
>><DIV>&nbsp;</DIV></BODY></HTML>
>>
>>------=_NextPart_000_0036_01BE70A4.15508AE0--
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>>
>Pablo Martin-Rabadan
>Hospital General Universitario Gregorio Maranon
>C/Doctor Esquerdo 46
>Madrid 28007
>FAX: 91 504 4906
>Tel: 91 586 8453
>     91 586 8910
>If calling from abroad delete first digit "9"
>Country code for Spain: 34
>
>
>