looks like amastigotes

Sun Apr 4 11:15:13 BRT 1999

Please share with the leishmaniacs the results of our suggestions:

Namely:

Does the organism seen in the smear, stain with silver (Grocott,
Silver-methenamine ...)

Patient clinical data and HIV serology

Bone marrow culture results for Leishmania/Trypanosome (NNN and Sneider),
mice intraperitoneal inoculation and fungal culture (Blood-BHI-agar)

	Commercially availlable monoclonal antibodies can be used for
inmunoflurescent staining in orther to rule-out Toxoplasma gondii.

Pablo

 13:45 17/03/99 -0300, you wrote:
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>Dear all;
>I will show you a photograph of bone marrow puncture slide stained by =
>Giemsa at (http://med.ege.edu.tr/~mkorkmaz/suspect.htm ). The patient =
>suspected as kala-azar and it was sended for searching Leishmania =
>amastigotes. There was no amastigotes? at the slide.  Leishmaniasis =
>serology (IFA,ELISA) negative.=20
>But I saw a different organism and showed it to microbiology and =
>pathology department. They could not give an idea about it. When I asked =
>could be Histoplasma capsulatum? They say no. Please help me if any one =
>knows this organism. Thank you in advance.
>
>Seray Ozensoy, MD
>
>
>------=_NextPart_000_0036_01BE70A4.15508AE0
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><!DOCTYPE HTML PUBLIC "-//W3C//DTD W3 HTML//EN">
><HTML><HEAD>
><META content=3D"text/html; charset=3Dwindows-1254" =
>http-equiv=3DContent-Type>
><META content=3D'"MSHTML 5.00.0910.1309"' name=3DGENERATOR></HEAD>
><BODY bgColor=3D#ffffff>
><DIV><FONT face=3DArial size=3D2>Dear all;</FONT></DIV>
><DIV><FONT face=3DArial size=3D2>I will show you a photograph of bone =
>marrow=20
>puncture slide stained by Giemsa at (<A=20
>href=3D"http://med.ege.edu.tr/~mkorkmaz/suspect.htm">http://med.ege.edu.t=
>r/~mkorkmaz/suspect.htm</A>=20
>). The patient suspected as kala-azar and it was sended for searching =
>Leishmania=20
>amastigotes. There was no amastigotes? at the slide.  Leishmaniasis =
>serology=20
>(IFA,ELISA) negative. </FONT></DIV>
><DIV><FONT face=3DArial size=3D2>But I saw a different organism and =
>showed it to=20
>microbiology and pathology department. They could not give an idea about =
>it.=20
>When I asked could be Histoplasma capsulatum? They say no. Please help =
>me if any=20
>one knows this organism. Thank you in advance.</FONT></DIV>
><DIV>&nbsp;</DIV>
><DIV><FONT face=3DArial size=3D2>Seray Ozensoy, MD</FONT></DIV>
><DIV>&nbsp;</DIV></BODY></HTML>
>
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>
Pablo Martin-Rabadan
Hospital General Universitario Gregorio Maranon
C/Doctor Esquerdo 46
Madrid 28007
FAX: 91 504 4906
Tel: 91 586 8453
     91 586 8910
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