Dear Dr. Alves, I wouldn't mind reading the protocol that you use to seperate pro- and Meta-cylics of L. amazonensis as well. Hannah Akuffo Hannah Akuffo Microbiology and Tumor Biology Centre (MTC) Karolinska Institute Box 280 S-17 177 Stockholm Sweden. Tel: 46 8 728 7236 Fax: 46 8 33 15 47