<html><head></head><body><div style="color:#000; background-color:#fff; font-family:verdana, helvetica, sans-serif;font-size:16px"><div id="yui_3_16_0_ym19_1_1463150644124_21361">Dear Hind</div><div id="yui_3_16_0_ym19_1_1463150644124_21360"><br></div><div id="yui_3_16_0_ym19_1_1463150644124_21245" dir="ltr">In Nepal, we are using QIAmp DNA minikit (51306, Qiagen, Germany) for DNA extraction from sandfly. In this kit, we do not observed inhibition of PCR, it works well.</div><div id="yui_3_16_0_ym19_1_1463150644124_21388" dir="ltr"><br></div><div id="yui_3_16_0_ym19_1_1463150644124_21416" dir="ltr">thanks</div><div id="yui_3_16_0_ym19_1_1463150644124_21417" dir="ltr"><br></div><div id="yui_3_16_0_ym19_1_1463150644124_21418" dir="ltr">Best regards</div><div id="yui_3_16_0_ym19_1_1463150644124_21419" dir="ltr">Narayan<br></div><div id="yui_3_16_0_ym19_1_1463150644124_21196"><span></span></div><div id="yui_3_16_0_ym19_1_1463150644124_21150" class="qtdSeparateBR"><br><br></div><div style="display: block;" id="yui_3_16_0_ym19_1_1463150644124_21155" class="yahoo_quoted"> <blockquote id="yui_3_16_0_ym19_1_1463150644124_21154" style="border-left: 2px solid rgb(16, 16, 255); margin-left: 5px; margin-top: 5px; padding-left: 5px;"> <div id="yui_3_16_0_ym19_1_1463150644124_21153" style="font-family: verdana, helvetica, sans-serif; font-size: 16px;"> <div id="yui_3_16_0_ym19_1_1463150644124_21152" style="font-family: HelveticaNeue, Helvetica Neue, Helvetica, Arial, Lucida Grande, sans-serif; font-size: 16px;"> <div id="yui_3_16_0_ym19_1_1463150644124_21151" dir="ltr"> <font id="yui_3_16_0_ym19_1_1463150644124_21158" face="Arial" size="2"> <hr size="1"> <b id="yui_3_16_0_ym19_1_1463150644124_21157"><span id="yui_3_16_0_ym19_1_1463150644124_21156" style="font-weight:bold;">From:</span></b> "hindabushama@uofk.edu" <hindabushama@uofk.edu><br> <b><span style="font-weight: bold;">To:</span></b> "David, John R." <jdavid@hsph.harvard.edu> <br><b><span style="font-weight: bold;">Cc:</span></b> Leish-L <Leish-l@lineu.icb.usp.br><br> <b><span style="font-weight: bold;">Sent:</span></b> Wednesday, May 11, 2016 6:00 PM<br> <b><span style="font-weight: bold;">Subject:</span></b> Re: [Leish-l] Fwd: Leishmaniasis - sand fly or sandfly or sand-fly?<br> </font> </div> <div class="y_msg_container"><br><div id="yiv5578658157"><div>
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<div>Dear All </div>
<div>Iam looking for a reliable method for DNA extraction from individual sandfly which will give a good DNA that could be used in PCR </div>
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Best Regards</div>
<div style="font-size:12pt;color:#000000;background-color:#ffffff;font-family:Calibri, Arial, Helvetica, sans-serif;">Hind<div class="yiv5578658157yqt5647824826" id="yiv5578658157yqtfd52994"><br clear="none"><br clear="none">
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<div dir="ltr" id="yiv5578658157divRplyFwdMsg"><span style="font-size:11pt;color:#000000;font-family:Calibri, sans-serif;"><strong>From:</strong> leish-l-bounces@lineu.icb.usp.br <leish-l-bounces@lineu.icb.usp.br> on behalf of Campos-Neto, Antonio <Acampos@forsyth.org><br clear="none"><strong>Sent:</strong> Monday, February 15, 2016 2:30 PM<br clear="none"><strong>To:</strong> Carlos Costa; Leish-L<br clear="none"><strong>Subject:</strong> Re: [Leish-l] Fwd: Leishmaniasis - sand fly or sandfly or sand-fly?</span>
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<div style="margin:0in 0in 0.0001pt;font-size:12pt;"><span style="font-size:11.0pt;font-family:'Calibri', 'sans-serif';color:#1f497d;">Well, I have to add the Equator divide to the Atlantic Ocean divide in the conversation about sandfly versus sand fly. Below the Equator and on the Western of the Atlantic Ocean, any little thing that flies and bites are called erroneously, I recognize, Mosquito, not Flies </span><span style="font-size:11.0pt;font-family:Wingdings;color:#1f497d;">J</span><span style="font-size:11.0pt;font-family:'Calibri', 'sans-serif';color:#1f497d;">.</span></div>
<div style="margin:0in 0in 0.0001pt;font-size:12pt;"><span style="font-size:11.0pt;font-family:'Calibri', 'sans-serif';color:#1f497d;">Fortunately, for our papers we do not have to add this third linguistic quandary and have to deal only with the dilemma of sand fly versus sandfly.</span></div>
<div style="margin:0in 0in 0.0001pt;font-size:12pt;"><span style="font-size:11.0pt;font-family:'Calibri', 'sans-serif';color:#1f497d;">Best to all.</span></div>
<div style="margin:0in 0in 0.0001pt;font-size:12pt;"><span style="font-size:11.0pt;font-family:'Calibri', 'sans-serif';color:#1f497d;">acn</span></div>
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<div style="margin:0in 0in 0.0001pt;font-size:12pt;"><span style="font-size:10.0pt;font-family:'Arial', 'sans-serif';color:#365f91;">Antonio Campos-Neto, MD</span><span style="font-family:'Arial', 'sans-serif';color:#1f497d;"></span></div>
<div style="margin:0in 0in 0.0001pt;font-size:12pt;"><span style="font-size:10.0pt;font-family:'Arial', 'sans-serif';color:#365f91;">Director, Global Infectious Disease Research Center</span><span style="color:#1f497d;"></span></div>
<div style="margin:0in 0in 0.0001pt;font-size:12pt;"><span style="font-size:10.0pt;font-family:'Arial', 'sans-serif';color:#365f91;">The Forsyth Institute</span><span style="color:#1f497d;"></span></div>
<div style="margin:0in 0in 0.0001pt;font-size:12pt;"><span style="font-size:10.0pt;font-family:'Arial', 'sans-serif';color:#365f91;">245 First Street</span><span style="color:#1f497d;"></span></div>
<div style="margin:0in 0in 0.0001pt;font-size:12pt;"><span style="font-size:10.0pt;font-family:'Arial', 'sans-serif';color:#365f91;">Cambridge, MA 02142</span><span style="color:#1f497d;"></span></div>
<div style="margin:0in 0in 0.0001pt;font-size:12pt;"><span style="font-size:10.0pt;font-family:'Arial', 'sans-serif';color:#365f91;">Phone: (617) 892-8393</span><span style="color:#1f497d;"></span></div>
<div style="margin:0in 0in 0.0001pt;font-size:12pt;"><span style="font-size:10.0pt;font-family:'Arial', 'sans-serif';color:#365f91;">EM: <a rel="nofollow" shape="rect" style="color:blue;text-decoration:underline;" ymailto="mailto:acampos@forsyth.org" target="_blank" href="mailto:acampos@forsyth.org"> acampos@forsyth.org</a> </span><span style="color:#1f497d;"></span></div>
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<div style="margin:0in 0in 0.0001pt;font-size:12pt;">---------- Forwarded message ----------<br clear="none"> From: <strong>Anthony Bryceson</strong> <<a rel="nofollow" shape="rect" style="color:blue;text-decoration:underline;" ymailto="mailto:a.bryceson@doctors.net.uk" target="_blank" href="mailto:a.bryceson@doctors.net.uk">a.bryceson@doctors.net.uk</a>><br clear="none"> Date: 2009-03-30 21:06 GMT-03:00<br clear="none"> Subject: Re: [Leish-l] Leishmaniasis - sand fly or sandfly or sand-fly?<br clear="none"> To: BobKillick-Kendrick <<a rel="nofollow" shape="rect" style="color:blue;text-decoration:underline;" ymailto="mailto:killickendrick@wanadoo.fr" target="_blank" href="mailto:killickendrick@wanadoo.fr">killickendrick@wanadoo.fr</a>><br clear="none"> Cc: Carlos Costa <<a rel="nofollow" shape="rect" style="color:blue;text-decoration:underline;" ymailto="mailto:chncosta@gmail.com" target="_blank" href="mailto:chncosta@gmail.com">chncosta@gmail.com</a>>, "Chang, Kwang-Poo" <<a rel="nofollow" shape="rect" style="color:blue;text-decoration:underline;" ymailto="mailto:KwangPoo.Chang@rosalindfranklin.edu" target="_blank" href="mailto:KwangPoo.Chang@rosalindfranklin.edu">KwangPoo.Chang@rosalindfranklin.edu</a>>, fred opperdoes <<a rel="nofollow" shape="rect" style="color:blue;text-decoration:underline;" ymailto="mailto:fred.opperdoes@uclouvain.be" target="_blank" href="mailto:fred.opperdoes@uclouvain.be">fred.opperdoes@uclouvain.be</a>>, <a rel="nofollow" shape="rect" style="color:blue;text-decoration:underline;" ymailto="mailto:Leish-l@lineu.icb.usp.br" target="_blank" href="mailto:Leish-l@lineu.icb.usp.br"> Leish-l@lineu.icb.usp.br</a><br clear="none"><br clear="none"></div>
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<div style="margin:0in 0in 0.0001pt;font-size:12pt;">The academics have spoken, but us lay writers could be forgiven for getting it "wrong". Usage accepts sandfly on this side of the Atlantic. As Bob pointed out the divide between sandfly and sand-fly is the Atlantic Ocean. The Oxford English Dictionary and Collins offer only sandfly, Webster offers only sand fly. Encyclopedia Britannica uses sand fly, but then includes midges and gnats. Wikipedia is utterly confused. Trawling the publications of the famous ento- parasito-logists showed that British journals such as TRSTMH use sandfly, Continental journals mostly use sandfly when publishing in English, but some such as Parassitologia seem a little more flexible and use either. US journals use sand fly, but adopted the style at different times: AJTMH in abut 1983, Science and Mem Inst Oswaldo Cruz by about 2000. I found only one sand-fly. So select your journal carefully if you have strong feelings.</div>
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<div style="margin:0in 0in 0.0001pt;font-size:12pt;"><span style="font-family:'Helvetica', 'sans-serif';color:black;">Anthony Bryceson</span></div>
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<div style="margin:0in 0in 0.0001pt;font-size:12pt;">On 25 Mar 2009, at 07:00, BobKillick-Kendrick wrote:</div>
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<div style="margin:0in 0in 0.0001pt;font-size:12pt;"><span style="font-family:'Helvetica', 'sans-serif';color:black;">The Americans use sand fly. The convention is that two words indicate a dipteran - eg. sand fly, tsetse fly, horse fly, stable fly etc, - whereas one word indicates a non-dipteran - eg. mayfly, damselfly, hoverfly etc. It seems sensible to me and I follow it. [This is opposed by Chris Schofield. But he works on reduvidbugs (conenosebugs)!]</span></div>
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<div style="margin:0in 0in 0.0001pt;font-size:12pt;"><span style="font-family:'Helvetica', 'sans-serif';color:black;">Bob Killick-Kendrick</span></div>
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<div style="margin:0in 0in 0.0001pt;font-size:12pt;"><span style="font-size:10.0pt;font-family:'Arial', 'sans-serif';color:black;">----- Original Message -----</span></div>
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<div style="background-color:#e4e4e4;margin:0in 0in 0.0001pt;font-size:12pt;"><strong><span style="font-size:10.0pt;font-family:'Arial', 'sans-serif';color:black;">From:</span></strong><span style="font-size:10.0pt;font-family:'Arial', 'sans-serif';color:black;"> <a title-off="" rel="nofollow" shape="rect" style="color:blue;text-decoration:underline;" title="chncosta@gmail.com" ymailto="mailto:chncosta@gmail.com" target="_blank" href="mailto:chncosta@gmail.com">Carlos Costa</a></span></div>
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<div style="margin:0in 0in 0.0001pt;font-size:12pt;"><strong><span style="font-size:10.0pt;font-family:'Arial', 'sans-serif';color:black;">To:</span></strong><span style="font-size:10.0pt;font-family:'Arial', 'sans-serif';color:black;"> <a title-off="" rel="nofollow" shape="rect" style="color:blue;text-decoration:underline;" title="KwangPoo.Chang@rosalindfranklin.edu" ymailto="mailto:KwangPoo.Chang@rosalindfranklin.edu" target="_blank" href="mailto:KwangPoo.Chang@rosalindfranklin.edu">Chang, Kwang-Poo</a></span></div>
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<div style="margin:0in 0in 0.0001pt;font-size:12pt;"><strong><span style="font-size:10.0pt;font-family:'Arial', 'sans-serif';color:black;">Cc:</span></strong><span style="font-size:10.0pt;font-family:'Arial', 'sans-serif';color:black;"> <a title-off="" rel="nofollow" shape="rect" style="color:blue;text-decoration:underline;" title="fred.opperdoes@uclouvain.be" ymailto="mailto:fred.opperdoes@uclouvain.be" target="_blank" href="mailto:fred.opperdoes@uclouvain.be">fred opperdoes</a> ; <a title-off="" rel="nofollow" shape="rect" style="color:blue;text-decoration:underline;" title="Leish-l@lineu.icb.usp.br" ymailto="mailto:Leish-l@lineu.icb.usp.br" target="_blank" href="mailto:Leish-l@lineu.icb.usp.br">Leish-l@lineu.icb.usp.br</a></span></div>
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<div style="margin:0in 0in 0.0001pt;font-size:12pt;"><strong><span style="font-size:10.0pt;font-family:'Arial', 'sans-serif';color:black;">Sent:</span></strong><span style="font-size:10.0pt;font-family:'Arial', 'sans-serif';color:black;"> Tuesday, March 24, 2009 8:46 PM</span></div>
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<div style="margin:0in 0in 0.0001pt;font-size:12pt;"><strong><span style="font-size:10.0pt;font-family:'Arial', 'sans-serif';color:black;">Subject:</span></strong><span style="font-size:10.0pt;font-family:'Arial', 'sans-serif';color:black;"> Re: [Leish-l] Leishmaniasis - Argentina: epidemic potential</span></div>
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<div style="margin:0in 0in 0.0001pt;font-size:12pt;"><span style="font-family:'Helvetica', 'sans-serif';color:black;">To: all</span></div>
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<div style="margin:0in 0in 0.0001pt;font-size:12pt;"><span style="font-family:'Helvetica', 'sans-serif';color:black;">By the way, what is the right English writing: sand fly or sandfly? Is it a matter of England vs. US?</span></div>
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<div style="margin:0in 0in 0.0001pt;font-size:12pt;"><span style="font-family:'Helvetica', 'sans-serif';color:black;">Carlos H.</span></div>
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