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<p>Dear All </p>
<p>Iam looking for a reliable method for DNA extraction from individual sandfly which will give a good DNA that could be used in PCR </p>
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Best Regards</div>
<div style="font-size: 12pt; color: #000000; background-color: #ffffff; font-family: Calibri,Arial,Helvetica,sans-serif;">Hind<br /><br />
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<div id="divRplyFwdMsg" dir="ltr"><span style="font-size: 11pt; color: #000000; font-family: Calibri,sans-serif;"><strong>From:</strong> leish-l-bounces@lineu.icb.usp.br <leish-l-bounces@lineu.icb.usp.br> on behalf of Campos-Neto, Antonio <Acampos@forsyth.org><br /><strong>Sent:</strong> Monday, February 15, 2016 2:30 PM<br /><strong>To:</strong> Carlos Costa; Leish-L<br /><strong>Subject:</strong> Re: [Leish-l] Fwd: Leishmaniasis - sand fly or sandfly or sand-fly?</span>
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<p style="margin: 0in 0in 0.0001pt; font-size: 12pt;"><span style="font-size: 11.0pt; font-family: 'Calibri','sans-serif'; color: #1f497d;">Well, I have to add the Equator divide to the Atlantic Ocean divide in the conversation about sandfly versus sand fly. Below the Equator and on the Western of the Atlantic Ocean, any little thing that flies and bites are called erroneously, I recognize, Mosquito, not Flies </span><span style="font-size: 11.0pt; font-family: Wingdings; color: #1f497d;">J</span><span style="font-size: 11.0pt; font-family: 'Calibri','sans-serif'; color: #1f497d;">.</span></p>
<p style="margin: 0in 0in 0.0001pt; font-size: 12pt;"><span style="font-size: 11.0pt; font-family: 'Calibri','sans-serif'; color: #1f497d;">Fortunately, for our papers we do not have to add this third linguistic quandary and have to deal only with the dilemma of sand fly versus sandfly.</span></p>
<p style="margin: 0in 0in 0.0001pt; font-size: 12pt;"><span style="font-size: 11.0pt; font-family: 'Calibri','sans-serif'; color: #1f497d;">Best to all.</span></p>
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<p style="margin: 0in 0in 0.0001pt; font-size: 12pt;"><span style="font-size: 10.0pt; font-family: 'Arial','sans-serif'; color: #365f91;">Antonio Campos-Neto, MD</span><span style="font-family: 'Arial','sans-serif'; color: #1f497d;"></span></p>
<p style="margin: 0in 0in 0.0001pt; font-size: 12pt;"><span style="font-size: 10.0pt; font-family: 'Arial','sans-serif'; color: #365f91;">Director, Global Infectious Disease Research Center</span><span style="color: #1f497d;"></span></p>
<p style="margin: 0in 0in 0.0001pt; font-size: 12pt;"><span style="font-size: 10.0pt; font-family: 'Arial','sans-serif'; color: #365f91;">The Forsyth Institute</span><span style="color: #1f497d;"></span></p>
<p style="margin: 0in 0in 0.0001pt; font-size: 12pt;"><span style="font-size: 10.0pt; font-family: 'Arial','sans-serif'; color: #365f91;">245 First Street</span><span style="color: #1f497d;"></span></p>
<p style="margin: 0in 0in 0.0001pt; font-size: 12pt;"><span style="font-size: 10.0pt; font-family: 'Arial','sans-serif'; color: #365f91;">Cambridge, MA 02142</span><span style="color: #1f497d;"></span></p>
<p style="margin: 0in 0in 0.0001pt; font-size: 12pt;"><span style="font-size: 10.0pt; font-family: 'Arial','sans-serif'; color: #365f91;">Phone: (617) 892-8393</span><span style="color: #1f497d;"></span></p>
<p style="margin: 0in 0in 0.0001pt; font-size: 12pt;"><span style="font-size: 10.0pt; font-family: 'Arial','sans-serif'; color: #365f91;">EM: <a style="color: blue; text-decoration: underline;" href="mailto:acampos@forsyth.org"> acampos@forsyth.org</a> </span><span style="color: #1f497d;"></span></p>
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<p style="margin: 0in 0in 0.0001pt; font-size: 12pt;">---------- Forwarded message ----------<br /> From: <strong>Anthony Bryceson</strong> <<a style="color: blue; text-decoration: underline;" href="mailto:a.bryceson@doctors.net.uk">a.bryceson@doctors.net.uk</a>><br /> Date: 2009-03-30 21:06 GMT-03:00<br /> Subject: Re: [Leish-l] Leishmaniasis - sand fly or sandfly or sand-fly?<br /> To: BobKillick-Kendrick <<a style="color: blue; text-decoration: underline;" href="mailto:killickendrick@wanadoo.fr">killickendrick@wanadoo.fr</a>><br /> Cc: Carlos Costa <<a style="color: blue; text-decoration: underline;" href="mailto:chncosta@gmail.com">chncosta@gmail.com</a>>, "Chang, Kwang-Poo" <<a style="color: blue; text-decoration: underline;" href="mailto:KwangPoo.Chang@rosalindfranklin.edu">KwangPoo.Chang@rosalindfranklin.edu</a>>, fred opperdoes <<a style="color: blue; text-decoration: underline;" href="mailto:fred.opperdoes@uclouvain.be">fred.opperdoes@uclouvain.be</a>>, <a style="color: blue; text-decoration: underline;" href="mailto:Leish-l@lineu.icb.usp.br"> Leish-l@lineu.icb.usp.br</a><br /><br /></p>
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<p style="margin: 0in 0in 0.0001pt; font-size: 12pt;">The academics have spoken, but us lay writers could be forgiven for getting it "wrong". Usage accepts sandfly on this side of the Atlantic. As Bob pointed out the divide between sandfly and sand-fly is the Atlantic Ocean. The Oxford English Dictionary and Collins offer only sandfly, Webster offers only sand fly. Encyclopedia Britannica uses sand fly, but then includes midges and gnats. Wikipedia is utterly confused. Trawling the publications of the famous ento- parasito-logists showed that British journals such as TRSTMH use sandfly, Continental journals mostly use sandfly when publishing in English, but some such as Parassitologia seem a little more flexible and use either. US journals use sand fly, but adopted the style at different times: AJTMH in abut 1983, Science and Mem Inst Oswaldo Cruz by about 2000. I found only one sand-fly. So select your journal carefully if you have strong feelings.</p>
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<p style="margin: 0in 0in 0.0001pt; font-size: 12pt;"><span style="font-family: 'Helvetica','sans-serif'; color: black;">Anthony Bryceson</span></p>
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<p style="margin: 0in 0in 0.0001pt; font-size: 12pt;">On 25 Mar 2009, at 07:00, BobKillick-Kendrick wrote:</p>
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<p style="margin: 0in 0in 0.0001pt; font-size: 12pt;"><span style="font-family: 'Helvetica','sans-serif'; color: black;">The Americans use sand fly. The convention is that two words indicate a dipteran - eg. sand fly, tsetse fly, horse fly, stable fly etc, - whereas one word indicates a non-dipteran - eg. mayfly, damselfly, hoverfly etc. It seems sensible to me and I follow it. [This is opposed by Chris Schofield. But he works on reduvidbugs (conenosebugs)!]</span></p>
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<p style="margin: 0in 0in 0.0001pt; font-size: 12pt;"><span style="font-family: 'Helvetica','sans-serif'; color: black;">Bob Killick-Kendrick</span></p>
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<p style="margin: 0in 0in 0.0001pt; font-size: 12pt;"><span style="font-size: 10.0pt; font-family: 'Arial','sans-serif'; color: black;">----- Original Message -----</span></p>
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<p style="background-color: #e4e4e4; margin: 0in 0in 0.0001pt; font-size: 12pt;"><strong><span style="font-size: 10.0pt; font-family: 'Arial','sans-serif'; color: black;">From:</span></strong><span style="font-size: 10.0pt; font-family: 'Arial','sans-serif'; color: black;"> <a style="color: blue; text-decoration: underline;" title="chncosta@gmail.com" href="mailto:chncosta@gmail.com">Carlos Costa</a></span></p>
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<p style="margin: 0in 0in 0.0001pt; font-size: 12pt;"><strong><span style="font-size: 10.0pt; font-family: 'Arial','sans-serif'; color: black;">To:</span></strong><span style="font-size: 10.0pt; font-family: 'Arial','sans-serif'; color: black;"> <a style="color: blue; text-decoration: underline;" title="KwangPoo.Chang@rosalindfranklin.edu" href="mailto:KwangPoo.Chang@rosalindfranklin.edu">Chang, Kwang-Poo</a></span></p>
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<p style="margin: 0in 0in 0.0001pt; font-size: 12pt;"><strong><span style="font-size: 10.0pt; font-family: 'Arial','sans-serif'; color: black;">Cc:</span></strong><span style="font-size: 10.0pt; font-family: 'Arial','sans-serif'; color: black;"> <a style="color: blue; text-decoration: underline;" title="fred.opperdoes@uclouvain.be" href="mailto:fred.opperdoes@uclouvain.be">fred opperdoes</a> ; <a style="color: blue; text-decoration: underline;" title="Leish-l@lineu.icb.usp.br" href="mailto:Leish-l@lineu.icb.usp.br">Leish-l@lineu.icb.usp.br</a></span></p>
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<p style="margin: 0in 0in 0.0001pt; font-size: 12pt;"><strong><span style="font-size: 10.0pt; font-family: 'Arial','sans-serif'; color: black;">Sent:</span></strong><span style="font-size: 10.0pt; font-family: 'Arial','sans-serif'; color: black;"> Tuesday, March 24, 2009 8:46 PM</span></p>
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<p style="margin: 0in 0in 0.0001pt; font-size: 12pt;"><strong><span style="font-size: 10.0pt; font-family: 'Arial','sans-serif'; color: black;">Subject:</span></strong><span style="font-size: 10.0pt; font-family: 'Arial','sans-serif'; color: black;"> Re: [Leish-l] Leishmaniasis - Argentina: epidemic potential</span></p>
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<p style="margin: 0in 0in 0.0001pt; font-size: 12pt;"><span style="font-family: 'Helvetica','sans-serif'; color: black;">To: all</span></p>
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<p style="margin: 0in 0in 0.0001pt; font-size: 12pt;"><span style="font-family: 'Helvetica','sans-serif'; color: black;">By the way, what is the right English writing: sand fly or sandfly? Is it a matter of England vs. US?</span></p>
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<p style="margin: 0in 0in 0.0001pt; font-size: 12pt;"><span style="font-family: 'Helvetica','sans-serif'; color: black;">Carlos H.</span></p>
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<p style="margin: 0in 0in 0.0001pt; font-size: 12pt;"><span style="font-family: 'Verdana','sans-serif';">Carlos H. N. Costa, MD, DSc.</span></p>
<p style="margin: 0in 0in 0.0001pt; font-size: 12pt;"><span style="font-family: 'Verdana','sans-serif';">Coordenador</span></p>
<p style="margin: 0in 0in 0.0001pt; font-size: 12pt;"><span style="font-family: 'Verdana','sans-serif';">Laboratório de Leishmanioses - LabLeish</span></p>
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