<div dir="ltr">Dear Vahiden,<div><br></div><div>It is difficult to associate DAT antibody titers with LD loads determined by PCR for kDNA and ITS-1 in patient blood samples. Antibodies are often said to offer no protection in leishmaniasis ? </div>
<div><br></div><div>I don't know enough details to comment on both assays, especially DAT, e. g. the antigens used. The PCR based on the primers used is adequate for qualitative interpretation for the presence of Leishmania. They are expected to exist in the phagosome-lysosome vacuolar system of macrophages, which is expected to contain DNAse in abundance. One would not expect persistence of any DNA too long in that site.</div>
<div><br></div><div>May I asked you some questions ?</div><div><br></div><div>1. Is it easy to grow promastigotes from your samples ?</div><div>2. Are your patients all new cases in the clinic ? Have you come across patients who were diagnosed to have VL and cured many years ago ?</div>
<div><br></div><div>KP</div></div><div class="gmail_extra"><br><br><div class="gmail_quote">On Wed, Jan 29, 2014 at 4:19 AM, Vahideh Moin Vaziri <span dir="ltr"><<a href="mailto:vmvaziri@gmail.com" target="_blank">vmvaziri@gmail.com</a>></span> wrote:<br>
<blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex"><div>Dear Friends</div>
<div>Would you please help me to find the answer of following question kindly?</div>
<div> </div>
<div>Working on visceral leishmaniasis in a newly emerged hypoendemic foci in Iran we found seropositive human blood samples at different titres by DAT. To characterize parasite, kDNA and ITS1 PCR were done. The result showed that in the samples with lower titer ( 1/400, 1/800) parasite were detected by PCR much more than ones with high titer ( 1/3200).</div>
<div>I want to know, how long <em>L. infantum</em> will persist in human blood after infection? I mean how long the DNA of parasite could be detected by PCR? Is it right to say that at the time that we find high titer of antibody, parasite vanish from blood or not?</div>
<div> </div>
<div>Your kind cooperation is appreciated in advance.</div>
<div>Best wishes</div>
<div>-- <br>Vahideh Moin-Vaziri, Ph.D<br>Department of Parasitology and Mycology,<br>School of Medicine,<br>Shahid Beheshti University of Medical Sciences,<br>Post Code: 19395-4719<br>Yemen Street,<br>Chamran Expressway,<br>
Tehran, Iran,<br>Tel: <a href="tel:%2B98%2021%2023872564" value="+982123872564" target="_blank">+98 21 23872564</a><br>Fax: <a href="tel:%2B98%2021%2022439962" value="+982122439962" target="_blank">+98 21 22439962</a><br>
E mail: <a href="mailto:vmvaziri@gmail.com" target="_blank">vmvaziri@gmail.com</a><br> <a href="mailto:vmvaziri@hotmail.com" target="_blank">vmvaziri@hotmail.com</a><br></div>
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