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<DIV><FONT size=2 face=Arial>Dear Ketty and Dr. Reale,</FONT></DIV>
<DIV><FONT size=2 face=Arial>I wold ube very pleased to have these protocols and
pdfs to test those in my Lab. I am dealing with bats samples and I notice
that some protocols do give an answer when we use pure cultured Leishmania
samples but not in biologicals tissues. Please put me in your list whe you send
it to the others!</FONT></DIV>
<DIV><FONT size=2 face=Arial><A
href="mailto:caris@fmva.unesp.br">caris@fmva.unesp.br</A></FONT></DIV>
<DIV><FONT size=2 face=Arial>Thanks a lot!</FONT></DIV>
<DIV><FONT size=2 face=Arial>Cáris</FONT> </DIV>
<BLOCKQUOTE
style="BORDER-LEFT: #000000 2px solid; PADDING-LEFT: 5px; PADDING-RIGHT: 0px; MARGIN-LEFT: 5px; MARGIN-RIGHT: 0px">
<DIV style="FONT: 10pt arial">----- Original Message ----- </DIV>
<DIV
style="FONT: 10pt arial; BACKGROUND: #e4e4e4; font-color: black"><B>From:</B>
<A title=kwangpoo.chang@rosalindfranklin.edu
href="mailto:kwangpoo.chang@rosalindfranklin.edu">Kwang-Poo Chang</A> </DIV>
<DIV style="FONT: 10pt arial"><B>To:</B> <A title=ksoteriadou@pasteur.gr
href="mailto:ksoteriadou@pasteur.gr">Σωτηριάδου Καίτη</A> </DIV>
<DIV style="FONT: 10pt arial"><B>Cc:</B> <A title=PhillipL@niaid.nih.gov
href="mailto:PhillipL@niaid.nih.gov">Lawyer, Phillip (NIH/NIAID) [E]</A> ; <A
title=leish-l-bounces@lineu.icb.usp.br
href="mailto:leish-l-bounces@lineu.icb.usp.br">leish-l-bounces@lineu.icb.usp.br</A>
; <A title=rossella.lelli@izssicilia.it
href="mailto:rossella.lelli@izssicilia.it">Rossella Colomba Lelli</A> ; <A
title=Leish-L@lineu.icb.usp.br
href="mailto:Leish-L@lineu.icb.usp.br">Leish-L</A> ; <A
title=caporalevincenzo@gmail.com
href="mailto:caporalevincenzo@gmail.com">caporalevincenzo@gmail.com</A> </DIV>
<DIV style="FONT: 10pt arial"><B>Sent:</B> Friday, November 01, 2013 2:59
PM</DIV>
<DIV style="FONT: 10pt arial"><B>Subject:</B> Re: [Leish-l] why is Leishmania
donovani restricted to humans inIndian subcontinent?</DIV>
<DIV><BR></DIV>
<DIV dir=ltr>Dear Ketty,
<DIV><BR></DIV>
<DIV>Thanks a lot for the information ! </DIV>
<DIV><BR></DIV>
<DIV>Could you kindly send the pdf reprints for k26, CBP and hsp90 PCR for
diagnostic discrimination of L donovani complex for clinical samples ? I
assume that they work for dog, sanfly and human samples ?</DIV>
<DIV><BR></DIV>
<DIV>KP</DIV></DIV>
<DIV class=gmail_extra><BR><BR>
<DIV class=gmail_quote>On Fri, Nov 1, 2013 at 5:34 AM, Σωτηριάδου Καίτη <SPAN
dir=ltr><<A href="mailto:ksoteriadou@pasteur.gr"
target=_blank>ksoteriadou@pasteur.gr</A>></SPAN> wrote:<BR>
<BLOCKQUOTE
style="BORDER-LEFT: #ccc 1px solid; MARGIN: 0px 0px 0px 0.8ex; PADDING-LEFT: 1ex"
class=gmail_quote>
<DIV>
<DIV
style="FONT-FAMILY: HelveticaNeue,Helvetica Neue,Helvetica,Arial,Lucida Grande,sans-serif; FONT-SIZE: 12pt">
<DIV><SPAN>
<P style="MARGIN: 0cm 0cm 0pt" class=MsoNormal><SPAN
style="FONT-FAMILY: 'Calibri','sans-serif'; COLOR: #1f497d; FONT-SIZE: 11pt"
lang=EN-US></SPAN> </P><SPAN
style="FONT-FAMILY: 'Calibri','sans-serif'; COLOR: #1f497d; FONT-SIZE: 11pt"
lang=EN-US>
<P style="MARGIN: 0cm 0cm 0pt" class=MsoNormal><SPAN
style="FONT-FAMILY: 'Calibri','sans-serif'; COLOR: #1f497d; FONT-SIZE: 11pt"
lang=EN-US>Hi! In our hands also 13A/13B kDNA had the same problems. We came
to the conclusion that the balance sensitivity/specificity is not good.
Specifity/contamination is an issue in that
sense.<U></U><U></U><U></U></SPAN></P>
<P style="MARGIN: 0cm 0cm 0pt" class=MsoNormal><SPAN
style="FONT-FAMILY: 'Calibri','sans-serif'; COLOR: #1f497d; FONT-SIZE: 11pt"
lang=EN-US>If you want to discriminate species belonging to the L. donovani
complex k26-PCR, CPB and hsp90 give the same results that are not
always in agreement with MLEE. ITS (among others) is an excellent tool for
discriminating species causing VL or CL in Middle East. Please note that the
latter tools can be applied on clinical samples which is an important issue.
I could sent you the relevant papers in a pdf format or the citation, there
are several. Please let me know.<U></U><U></U></SPAN></P>
<P style="MARGIN: 0cm 0cm 0pt" class=MsoNormal><SPAN
style="FONT-FAMILY: 'Calibri','sans-serif'; COLOR: #1f497d; FONT-SIZE: 11pt"
lang=EN-US>Ketty Soteriadou (Greece)<U></U><U></U></SPAN></P>
<P style="MARGIN: 0cm 0cm 0pt"
class=MsoNormal></P></SPAN> </SPAN></DIV>
<DIV>
<DIV class=h5>
<DIV><SPAN></SPAN> </DIV>
<DIV><SPAN>The one by Reale is the 13A/13B kDNA protocol. Its extremely
sensitive. We've tried it. The problem is that after sometime, one start to
see that its becoming positive for all samples. Then we gave it up for the
benefit of genomic-based methods. Perhaps we should have used UNG-uracil in
the PCR setup as an anti-contamination agent.</SPAN></DIV>
<DIV
style="BACKGROUND-COLOR: transparent; FONT-STYLE: normal; FONT-FAMILY: HelveticaNeue,Helvetica Neue,Helvetica,Arial,Lucida Grande,sans-serif; FONT-SIZE: 16px"><BR><SPAN></SPAN></DIV>
<DIV
style="BACKGROUND-COLOR: transparent; FONT-STYLE: normal; FONT-FAMILY: HelveticaNeue,Helvetica Neue,Helvetica,Arial,Lucida Grande,sans-serif; FONT-SIZE: 16px"><SPAN>Amer</SPAN></DIV>
<DIV
style="BACKGROUND-COLOR: transparent; FONT-STYLE: normal; FONT-FAMILY: HelveticaNeue,Helvetica Neue,Helvetica,Arial,Lucida Grande,sans-serif; FONT-SIZE: 16px"><SPAN>Palestine<BR></SPAN></DIV>
<DIV
style="BACKGROUND-COLOR: transparent; FONT-STYLE: normal; FONT-FAMILY: HelveticaNeue,Helvetica Neue,Helvetica,Arial,Lucida Grande,sans-serif; FONT-SIZE: 16px"><SPAN><BR></SPAN></DIV>
<DIV> </DIV>
<DIV><BR></DIV>
<DIV><BR></DIV>
<DIV
style="FONT-FAMILY: HelveticaNeue,Helvetica Neue,Helvetica,Arial,Lucida Grande,sans-serif; FONT-SIZE: 12pt">
<DIV
style="FONT-FAMILY: HelveticaNeue,Helvetica Neue,Helvetica,Arial,Lucida Grande,sans-serif; FONT-SIZE: 12pt">
<DIV dir=ltr>
<HR SIZE=1>
<FONT face=Arial><B><SPAN style="FONT-WEIGHT: bold">From:</SPAN></B> Stefano
Reale <<A href="mailto:stefano.reale@izssicilia.it"
target=_blank>stefano.reale@izssicilia.it</A>><BR><B><SPAN
style="FONT-WEIGHT: bold">To:</SPAN></B> Kwang-Poo Chang <<A
href="mailto:kwangpoo.chang@rosalindfranklin.edu"
target=_blank>kwangpoo.chang@rosalindfranklin.edu</A>> <BR><B><SPAN
style="FONT-WEIGHT: bold">Cc:</SPAN></B> "Lawyer, Phillip (NIH/NIAID) [E]"
<<A href="mailto:PhillipL@niaid.nih.gov"
target=_blank>PhillipL@niaid.nih.gov</A>>; "<A
href="mailto:leish-l-bounces@lineu.icb.usp.br"
target=_blank>leish-l-bounces@lineu.icb.usp.br</A>" <<A
href="mailto:leish-l-bounces@lineu.icb.usp.br"
target=_blank>leish-l-bounces@lineu.icb.usp.br</A>>; Rossella Colomba
Lelli <<A href="mailto:rossella.lelli@izssicilia.it"
target=_blank>rossella.lelli@izssicilia.it</A>>; Leish-L <<A
href="mailto:Leish-L@lineu.icb.usp.br"
target=_blank>Leish-L@lineu.icb.usp.br</A>>; "<A
href="mailto:caporalevincenzo@gmail.com"
target=_blank>caporalevincenzo@gmail.com</A>" <<A
href="mailto:caporalevincenzo@gmail.com"
target=_blank>caporalevincenzo@gmail.com</A>> <BR><B><SPAN
style="FONT-WEIGHT: bold">Sent:</SPAN></B> Thursday, October 31, 2013 8:19
AM<BR><B><SPAN style="FONT-WEIGHT: bold">Subject:</SPAN></B> Re: [Leish-l]
why is Leishmania donovani restricted to humans in Indian
subcontinent?<BR></FONT></DIV>
<DIV><BR>
<DIV>
<DIV>
<DIV>Hallo Im one of the author of this PCR protocol. I optimised it by
specific primers and probe for infantum. It work very well and I showed the
Lod and Loq in variuos biological material taken from leishmaniotic dogs. In
my lab in Palermo I have a number if collected strains stored in liquid N2
ready to create the DNA standard referement serial dilutions fir Real time
test. I could help who should have these DNA.<BR clear=none>Best
regards</DIV>
<DIV>Il giorno 31/ott/2013 00:07, "Kwang-Poo Chang" <<A
href="mailto:kwangpoo.chang@rosalindfranklin.edu" shape=rect rel=nofollow
target=_blank>kwangpoo.chang@rosalindfranklin.edu</A>> ha scritto:<BR
clear=none>
<BLOCKQUOTE
style="BORDER-LEFT: #ccc 1px solid; MARGIN: 0px 0px 0px 0.8ex; PADDING-LEFT: 1ex">
<DIV dir=ltr>Dear Carlos,
<DIV><BR clear=none></DIV>
<DIV>One of my colleagues in Naples, Italy has used primer set for
kinetoplast minicircle conserved region, Considering ~10,000 copies of
minicircvles per Leishmania, this is probably the most sensitive PCR,
although the primer set can also amply all trypanosomes or trypanosomatid
protozoa (including Leptomonas reported to exist in the Indian kala-azar
splenic aspirates). Specificity could be an issue in that sense.</DIV>
<DIV><BR clear=none></DIV>
<DIV>KP </DIV></DIV>
<DIV><BR clear=none><BR clear=none>
<DIV>On Thu, Oct 10, 2013 at 7:31 PM, Carlos Lobo <SPAN dir=ltr><<A
href="mailto:carloshlobo@gmail.com" shape=rect rel=nofollow
target=_blank>carloshlobo@gmail.com</A>></SPAN> wrote:<BR clear=none>
<BLOCKQUOTE
style="BORDER-LEFT: #ccc 1px solid; MARGIN: 0px 0px 0px 0.8ex; PADDING-LEFT: 1ex">
<DIV dir=ltr>
<DIV>Hello guys, good night.</DIV>
<DIV><SPAN>I'm taking advantage
of</SPAN> <SPAN>this</SPAN> opportunity to <SPAN>exchange
</SPAN><SPAN>scientific</SPAN> <SPAN>information</SPAN><SPAN>,</SPAN>
<SPAN>much</SPAN> <SPAN>of which I</SPAN> <SPAN>have learned</SPAN>
<SPAN>to</SPAN> <SPAN>ask for</SPAN> <SPAN>help.</SPAN><BR
clear=none><SPAN>I'll</SPAN> <SPAN>start a</SPAN> <SPAN>project
on</SPAN> <SPAN>canine leishmaniasis</SPAN> <SPAN>in northeastern</SPAN>
<SPAN>Brazil</SPAN> <SPAN>and I</SPAN> <SPAN>need to diagnose,
by</SPAN> <SPAN>PCR,</SPAN> <SPAN>dogs</SPAN> <SPAN>infected
and</SPAN> <SPAN>not</SPAN> <SPAN>infected</SPAN> with
<SPAN>Leish</SPAN><SPAN>.</SPAN><BR clear=none><SPAN>I've been
doing</SPAN> <SPAN>some analysis</SPAN> <SPAN>of</SPAN> <SPAN>PCR
for</SPAN> <SPAN>other experiments</SPAN><SPAN>,</SPAN> <SPAN>but
never</SPAN> <SPAN>did for</SPAN> <SPAN>Leish</SPAN><SPAN>,</SPAN>
<SPAN>could someone give me</SPAN> <SPAN>some tips</SPAN><SPAN>? Like
which primer to use? Should I collect blood or tissue samples to have
more reliability?</SPAN><BR clear=none><SPAN>Thank
you</SPAN><SPAN>.</SPAN><BR clear=none><SPAN>Carlos
Henrique</SPAN></DIV></DIV>
<DIV>
<DIV>
<DIV><BR clear=none><BR clear=none>
<DIV>2013/9/23 Tamrat Abebe Zeleke <SPAN dir=ltr><<A
href="mailto:tabebezeleke@gmail.com" shape=rect rel=nofollow
target=_blank>tabebezeleke@gmail.com</A>></SPAN><BR clear=none>
<BLOCKQUOTE
style="BORDER-LEFT: #ccc 1px solid; MARGIN: 0px 0px 0px 0.8ex; PADDING-LEFT: 1ex">
<DIV dir=ltr>
<DIV style="FONT-FAMILY: georgia,serif">Dear Carlos,<BR clear=none><BR
clear=none></DIV>
<DIV style="FONT-FAMILY: georgia,serif">
<DIV>I agree with the comment given by Phillip Lawyer. The molecular
analysis of strains or isolates from India, Kenya, and South Western
Ethiopia also supports this notion. However, the issue of distinct
strains in Sudan and North Ethiopia opts for the fact that East Africa
may be the origin of at least the naughty L. donovani strains
circulating in the region. </DIV></DIV></DIV>
<DIV><BR clear=all>
<DIV>
<DIV dir=ltr>
<DIV><FONT face="tahoma, sans-serif"><SPAN
style="COLOR: rgb(111,168,220)">Tamrat Abebe</SPAN><BR
clear=none></FONT></DIV><FONT color=#6fa8dc><FONT
face="tahoma, sans-serif">Addis Ababa University College of Health
Sciences, School of Medicine ,<BR clear=none>Department of
Microbiology, Immunology & Parasitology </FONT></FONT>
<DIV><FONT color=#6fa8dc><FONT face="tahoma, sans-serif">Tikur Anbessa
Hospital</FONT></FONT></DIV>
<DIV><FONT color=#6fa8dc><FONT face="tahoma, sans-serif">Second floor
room number 76<BR clear=none>Tel: <A shape=rect rel=nofollow>+251 911
447227</A>(mobile)<BR clear=none>Email:</FONT><FONT
face="comic sans ms, sans-serif"> </FONT><A
href="mailto:tamrat.abebe@aau.edu.et" shape=rect rel=nofollow
target=_blank><FONT
face="tahoma, sans-serif">tamrat.abebe@aau.edu.et</FONT></A></FONT>
<DIV><FONT color=#6fa8dc face="comic sans ms, sans-serif"><B><BR
clear=none></B></FONT>
<DIV><FONT color=#666666 face="comic sans ms, sans-serif"><B><BR
clear=none></B></FONT> <BR clear=none><BR
clear=none> <BR
clear=none></DIV></DIV></DIV></DIV></DIV><BR clear=none><BR
clear=none>
<DIV>On Mon, Sep 16, 2013 at 4:37 AM, Lawyer, Phillip (NIH/NIAID) [E]
<SPAN dir=ltr><<A href="mailto:PhillipL@niaid.nih.gov" shape=rect
rel=nofollow target=_blank>PhillipL@niaid.nih.gov</A>></SPAN>
wrote:<BR clear=none>
<BLOCKQUOTE
style="BORDER-LEFT: rgb(204,204,204) 1px solid; MARGIN: 0px 0px 0px 0.8ex; PADDING-LEFT: 1ex">Dear
Carlos,<BR clear=none><BR clear=none><BR clear=none><BR
clear=none>For what it's worth, I believe it most likely happened
the other way around: Leishmania donovani was probably
introduced to East Africa from India during the late 1800s when
laborers were brought from India to work in the Kenya building the
railroad from Mombasa to Uganda and on other infrastructure
projects. Leishmania donovani in East Africa is manifest as
kala azar and is anthroponotic, the same as in India. The main
vector in Kenya is Phlebotmus martini, which tends to breed in
termite mounds, often associated with human dwellings. Other
Symphlebotomus species, Ph. vansomerenae and Ph. celiae have also
been implicated in L. donovani transmission.<BR clear=none><BR
clear=none><BR clear=none><BR clear=none>Regards,<BR clear=none><BR
clear=none><BR clear=none><BR clear=none>Phil Lawyer<BR
clear=none><BR clear=none>________________________________<BR
clear=none>From: Carlos Brisola Marcondes [<A
href="mailto:cbrisolamarcondes@gmail.com" shape=rect rel=nofollow
target=_blank>cbrisolamarcondes@gmail.com</A>]<BR clear=none>Sent:
Friday, August 30, 2013 8:41 AM<BR clear=none>To: Leish-L; <A
href="mailto:leish-l-bounces@lineu.icb.usp.br" shape=rect
rel=nofollow target=_blank>leish-l-bounces@lineu.icb.usp.br</A><BR
clear=none>Subject: [Leish-l] why is Leishmania donovani restricted
to humans in Indian subcontinent?<BR clear=none>
<DIV>
<DIV><BR clear=none>Dear all,<BR clear=none>Leishmania donovani
seems to have been introduced from East Africa to Indian
subcontinent, where it has infected mostly humans, differently from
East African foci.<BR clear=none> Why does this occur?
Is this caused by feeding preferences of Phlebotomus argentipes,
which bites mostly ruminants and humans and is associated to houses?
Or are dogs rarer in that region than in Brazil, where these animals
are important reservoirs of Leishmania infantum and frequently
bitten by Lutzomyia longipalpis?<BR clear=none><BR
clear=none>Sincerely yours<BR clear=none>prof. dr. Carlos Brisola
Marcondes<BR clear=none>Dept. Microbiol. Imunol. Parasitol./CCB<BR
clear=none>Federal University of Santa Catarina<BR
clear=none>Florianópolis (SC)<BR clear=none>CV: <A
href="http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783901J2"
shape=rect rel=nofollow
target=_blank>http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783901J2</A><BR
clear=none>blog: <A href="http://entomomedica.blogspot.com.br/"
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target=_blank>http://entomomedica.blogspot.com.br/</A><BR
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