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<body class='hmmessage'><div dir='ltr'>Dear Carlos<BR> <BR>If you want to detect canine leish caused for Viannia subgenus, the primers designed by Lopez M, Inga R, Cangalaya M, Echevarria J, Llanos-Cuentas A, Orrego C, Arevalo J. Diagnosis of Leishmania using the polymerase chain reaction: a simplified procedure for field work. Am J Trop Med Hyg. 1993 Sep;49(3):348-56. PubMed PMID: 8396860. are better than others.<BR>We have used these primers with human and canine samples from cutaneous leish with excellent results.<BR> <BR>Regards<BR> <BR>Dr. Maxy De los Santos D<BR>Department of Parasitology<BR>U.S.Naval Medical Research Unit 6 (NAMRU-6)<BR>Lima-Perú<BR><br> <BR><div>> Date: Sun, 20 Oct 2013 20:42:41 +0200<br>> From: patrick.bastien@univ-montp1.fr<br>> To: carloshlobo@gmail.com<br>> CC: Leish-L@lineu.icb.usp.br; leish-l-bounces@lineu.icb.usp.br<br>> Subject: Re: [Leish-l] primers for canine leishmaniasis<br>> <br>> Dear Carlos,<br>> Have a look at three excellent papers by Lachaud et al. (JCM 2001, JCM <br>> 2002 and Parasitol. .2002). Although they seem old, there is a lot of <br>> valuable information in it, and the things have not changed much. The <br>> primers by Mary et al. and Chargui et al. seem to have improved this <br>> PCR. (Obviously, this is a personal opinion, and certainly many <br>> researchers can write other things)<br>> If you cannot have access to the one in Parasitology, I can send it to you.<br>> In brief, if you use a repeated nuclear DNA target, you will have a <br>> robust sensitivity that will enable you to detect most 'sick' animals. <br>> If you use the kinetoplast minicircle, you will get a <br>> 'hyper-sensitivity" that will allow you detecting all infected animals <br>> (including asymptomatic ones) but that will necessitate drastic <br>> working conditions (as you will be exposed to an extreme sensitivity <br>> to carry-over contaminations).<br>> Although it has been used with success, I do not advise you to use a <br>> nested PCR for the same reasons.<br>> In any case, you have to 'optimize' your PCR using first parasite <br>> promastigotes and then using seeded 'mimicked' samples.<br>> Good luck !<br>> and best wishes<br>> P. Bastien<br>> <br>> Pr Patrick Bastien<br>> Director, Departement de Parasitologie-Mycologie<br>> Centre National de Reference des Leishmanioses<br>> Vice-Director, "MiVEGEC" unit research UMR5290 CNRS/224 IRD/UM1/UM2<br>> Faculte de Medecine, Université Montpellier 1<br>> 39 Av. Charles Flahault<br>> 34295 Montpellier cedex 5, France<br>> <br>> ----- Message de carloshlobo@gmail.com ---------<br>> Date : Thu, 10 Oct 2013 21:31:30 -0300<br>> De : Carlos Lobo <carloshlobo@gmail.com><br>> Répondre à : Carlos Lobo <carloshlobo@gmail.com><br>> Objet : Re: [Leish-l] why is Leishmania donovani restricted to <br>> humans in Indian subcontinent?<br>> À : Tamrat Abebe Zeleke <tabebezeleke@gmail.com><br>> Cc : Leish-L <Leish-L@lineu.icb.usp.br>, "Lawyer, Phillip <br>> (NIH/NIAID) [E]" <PhillipL@niaid.nih.gov>, <br>> "leish-l-bounces@lineu.icb.usp.br" <leish-l-bounces@lineu.icb.usp.br><br>> <br>> <br>> > Hello guys, good night.<br>> > I'm taking advantage of this opportunity to exchange scientific information,<br>> > much of which I have learned to ask for help.<br>> > I'll start a project on canine leishmaniasis in northeastern Brazil <br>> > and I need<br>> > to diagnose, by PCR, dogs infected and not infected with Leish.<br>> > I've been doing some analysis of PCR for other experiments, but never did<br>> > for Leish, could someone give me some tips? Like which primer to use?<br>> > Should I collect blood or tissue samples to have more reliability?<br>> > Thank you.<br>> > Carlos Henrique<br>> ><br>> ><br>> > 2013/9/23 Tamrat Abebe Zeleke <tabebezeleke@gmail.com><br>> ><br>> >> Dear Carlos,<br>> >><br>> >> I agree with the comment given by Phillip Lawyer. The molecular analysis<br>> >> of strains or isolates from India, Kenya, and South Western Ethiopia also<br>> >> supports this notion. However, the issue of distinct strains in Sudan and<br>> >> North Ethiopia opts for the fact that East Africa may be the origin of at<br>> >> least the naughty L. donovani strains circulating in the region.<br>> >><br>> >> Tamrat Abebe<br>> >> Addis Ababa University College of Health Sciences, School of Medicine ,<br>> >> Department of Microbiology, Immunology & Parasitology<br>> >> Tikur Anbessa Hospital<br>> >> Second floor room number 76<br>> >> Tel: +251 911 447227(mobile)<br>> >> Email: tamrat.abebe@aau.edu.et<br>> >> *<br>> >> *<br>> >> *<br>> >> *<br>> >><br>> >><br>> >><br>> >><br>> >> On Mon, Sep 16, 2013 at 4:37 AM, Lawyer, Phillip (NIH/NIAID) [E] <<br>> >> PhillipL@niaid.nih.gov> wrote:<br>> >><br>> >>> Dear Carlos,<br>> >>><br>> >>><br>> >>><br>> >>> For what it's worth, I believe it most likely happened the other way<br>> >>> around: Leishmania donovani was probably introduced to East Africa from<br>> >>> India during the late 1800s when laborers were brought from India to work<br>> >>> in the Kenya building the railroad from Mombasa to Uganda and on other<br>> >>> infrastructure projects. Leishmania donovani in East Africa is manifest as<br>> >>> kala azar and is anthroponotic, the same as in India. The main vector in<br>> >>> Kenya is Phlebotmus martini, which tends to breed in termite mounds, often<br>> >>> associated with human dwellings. Other Symphlebotomus species, Ph.<br>> >>> vansomerenae and Ph. celiae have also been implicated in L. donovani<br>> >>> transmission.<br>> >>><br>> >>><br>> >>><br>> >>> Regards,<br>> >>><br>> >>><br>> >>><br>> >>> Phil Lawyer<br>> >>><br>> >>> ________________________________<br>> >>> From: Carlos Brisola Marcondes [cbrisolamarcondes@gmail.com]<br>> >>> Sent: Friday, August 30, 2013 8:41 AM<br>> >>> To: Leish-L; leish-l-bounces@lineu.icb.usp.br<br>> >>> Subject: [Leish-l] why is Leishmania donovani restricted to humans in<br>> >>> Indian subcontinent?<br>> >>><br>> >>> Dear all,<br>> >>> Leishmania donovani seems to have been introduced from East Africa to<br>> >>> Indian subcontinent, where it has infected mostly humans, differently from<br>> >>> East African foci.<br>> >>> Why does this occur? Is this caused by feeding preferences of<br>> >>> Phlebotomus argentipes, which bites mostly ruminants and humans and is<br>> >>> associated to houses? Or are dogs rarer in that region than in Brazil,<br>> >>> where these animals are important reservoirs of Leishmania infantum and<br>> >>> frequently bitten by Lutzomyia longipalpis?<br>> >>><br>> >>> Sincerely yours<br>> >>> prof. dr. Carlos Brisola Marcondes<br>> >>> Dept. Microbiol. Imunol. Parasitol./CCB<br>> >>> Federal University of Santa Catarina<br>> >>> Florianópolis (SC)<br>> >>> CV: http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783901J2<br>> >>> blog: http://entomomedica.blogspot.com.br/<br>> >>><br>> >>><br>> >>> --<br>> >>> This email was sent by icb.usp.br ­­<br>> >>> _______________________________________________<br>> >>> Leish-l mailing list<br>> >>> Leish-l@lineu.icb.usp.br<br>> >>> http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l<br>> >>><br>> >>> --<br>> >>><br>> >>> This email was sent by icb.usp.br<br>> >>><br>> >><br>> >><br>> >><br>> >> --<br>> >> * This email was sent by icb.usp.br * ­­<br>> >> _______________________________________________<br>> >> Leish-l mailing list<br>> >> Leish-l@lineu.icb.usp.br<br>> >> http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l<br>> >><br>> >> --<br>> >> This email was sent by icb.usp.br<br>> >><br>> >><br>> ><br>> > --<br>> > This email was sent by icb.usp.br<br>> ><br>> <br>> <br>> ----- Fin du message de carloshlobo@gmail.com -----<br>> <br>> <br>> <br>> _______________________________________________<br>> Leish-l mailing list<br>> Leish-l@lineu.icb.usp.br<br>> http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l<br>> <br>> --<br>> <br>> This email was sent by icb.usp.br<br></div> </div></body>
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