NL Shamra reported his finding of rK39 dipstick seropositive dogs along the satluj River Valley, India (see his publication attached for your reference). <div><br></div><div>In Steve Reed's original article, K39 repeats of the kinesin genes were shown to be an amastigote-specific antigen of L donvoani/infantum.</div>
<div><br></div><div>rK39 seropositivity thus implies intracellular parasitism, but cannot result from "immunization" of animals with promastigotes via bites by infected vectors.</div><div><br></div><div>Our preliminary analysis of Dr. Sharma's CL samples suggests the presence of nagt sequences homologous to that of L infantum or L tropica or a mixture of both. </div>
<div><br></div><div>A lot of further studies in great detail by a multidisciplinary group of experts are absolutely essential in this and many other unexplored endemic sites, as mentioned.</div><div><br></div><div>KP <br>
<br><div class="gmail_quote">On Sat, Jun 22, 2013 at 1:06 AM, Mohammad Shafiul Alam <span dir="ltr"><<a href="mailto:shafiul@icddrb.org" target="_blank">shafiul@icddrb.org</a>></span> wrote:<br><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">
<div lang="EN-US" link="blue" vlink="purple">
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d">Dear All,<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d"><u></u> <u></u></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d">I think Dr. Anuradha’s observation is important. In rural Bangladesh you will find mostly stray dogs. Our vector
<i>P. argentipes</i> although preferably zoophilic I have never seen any report on its preference to canine blood. I’m missing the link here! In India and Bangladesh it was cattle to be the preferred host of P<i>. argentipes</i>. During our investigation with
cattle’s blood we found antibody (rK39 and DAT) but did not get any parasite (PCR) (</span><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d"><a href="http://www.biomedcentral.com/1746-6148/7/27" target="_blank"><span style="color:#1f497d">http://www.biomedcentral.com/1746-6148/7/27</span></a>).
However, there is a report from Nepal where L. donovani was found in cows, buffaloes and even in goats (PCR). I believe still we are far to establish an animal reservoir for VL in Indian subcontinent.
<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d"><u></u> <u></u></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d">Shafiul<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d"><u></u> <u></u></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d">Mohammad Shafiul Alam<br>
Assistant Scientist<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d">Parasitology Research Group<br>
ICDDR,B<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d">68 Shaheed Tajuddin Ahmed Sarani<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d">Mohakhali, Dhaka-1212<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d">Bangladesh<br>
Phone: +88 02 </span><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d">9827001-10</span><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d"> ext.3482<u></u><u></u></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d">Website:
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<p class="MsoNormal"><i><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d">Knowledge for Global Lifesaving Solutions<u></u><u></u></span></i></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d"><u></u> <u></u></span></p>
<p class="MsoNormal"> <span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d"><u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d"><u></u> <u></u></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d"><u></u> <u></u></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d"><u></u> <u></u></span></p>
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<div style="border:none;border-top:solid #b5c4df 1.0pt;padding:3.0pt 0in 0in 0in">
<p class="MsoNormal"><b><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif"">From:</span></b><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif""> <a href="mailto:leish-l-bounces@lineu.icb.usp.br" target="_blank">leish-l-bounces@lineu.icb.usp.br</a> [mailto:<a href="mailto:leish-l-bounces@lineu.icb.usp.br" target="_blank">leish-l-bounces@lineu.icb.usp.br</a>]
<b>On Behalf Of </b>Anuradha Dube<br>
<b>Sent:</b> Thursday, June 20, 2013 10:55 PM<br>
<b>To:</b> Syamal Roy; jeffrey shaw<br>
<b>Cc:</b> <a href="mailto:leish-l@lineu.icb.usp.br" target="_blank">leish-l@lineu.icb.usp.br</a></span></p><div><div class="h5"><br>
<b>Subject:</b> Re: [Leish-l] L.donovani in dogs?<u></u><u></u></div></div><p></p>
</div>
</div><div><div class="h5">
<p class="MsoNormal"><u></u> <u></u></p>
<div>
<p class="MsoNormal"><span style="font-family:"Calibri","sans-serif"">Dear Syamal<u></u><u></u></span></p>
<div>
<p class="MsoNormal"><span style="font-family:"Calibri","sans-serif""><u></u> <u></u></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-family:"Calibri","sans-serif"">I have tried to develop dog as a model for L. donovani ( Dd8 strain) while I was working on monkey model in late nineties. I have infected stray dogs but none were found positive on day 30
p.i. <u></u><u></u></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-family:"Calibri","sans-serif""><u></u> <u></u></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-family:"Calibri","sans-serif"">Anuradha<u></u><u></u></span></p>
</div>
<p class="MsoNormal"><span style="font-family:"Calibri","sans-serif""><u></u> <u></u></span></p>
<div>
<p class="MsoNormal"><span style="font-family:"Comic Sans MS"">ANURADHA DUBE, Ph.D <br>
Chief Scientist (Scientist G) ,<br>
Leishmania laboratory, Division of Parasitology <br>
Central Drug Research Institute <br>
Chattar Manzil Palace <br>
LUCKNOW-226001 Uttar Pradesh, INDIA <br>
Ph nos: OFF 522 2612411-18 EXT: 4398 Fax no: 522 2623405 <br>
RES: 2733454/4070667;mobile # 9450931332/9415104918; </span><span style="font-family:"Calibri","sans-serif""><u></u><u></u></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-family:"Comic Sans MS"">e-mail: </span><span style="font-family:"Calibri","sans-serif""><a href="mailto:anuradha_dube@hotmail.com;%20a_dube@cdri.res.in;" target="_blank"><span style="font-family:"Comic Sans MS"">anuradha_dube@hotmail.com;
anuradhadube@gmail.com; a_dube@cdri.res.in; </span></a> <br>
------------------------------------------------------------<u></u><u></u></span></p>
</div>
<p class="MsoNormal" style="margin-bottom:12.0pt"><span style="font-family:"Calibri","sans-serif""><u></u> <u></u></span></p>
<div>
<div class="MsoNormal" align="center" style="text-align:center"><span style="font-family:"Calibri","sans-serif"">
<hr size="2" width="100%" align="center">
</span></div>
<p class="MsoNormal" style="margin-bottom:12.0pt"><span style="font-family:"Calibri","sans-serif"">Date: Thu, 20 Jun 2013 08:35:37 +0530<br>
From: <a href="mailto:sroy@iicb.res.in" target="_blank">sroy@iicb.res.in</a><br>
To: <a href="mailto:jayusp@hotmail.com" target="_blank">jayusp@hotmail.com</a><br>
CC: <a href="mailto:leish-l@lineu.icb.usp.br" target="_blank">leish-l@lineu.icb.usp.br</a><br>
Subject: Re: [Leish-l] L.donovani in dogs?<u></u><u></u></span></p>
<div>
<div>
<p class="MsoNormal"><span style="font-family:"Calibri","sans-serif"">Dear Steve<u></u><u></u></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-family:"Calibri","sans-serif"">Dear Jeff:<u></u><u></u></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-family:"Calibri","sans-serif"">The information seems interesting. I do not know any such information about VL in dogs in India. India and Bangladesh border is porous and I would assume that may be there are cases of VL
infected dogs in India as well. How authentic this information is? Do they have any data? May I have it?<u></u><u></u></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-family:"Calibri","sans-serif"">Best<u></u><u></u></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-family:"Calibri","sans-serif"">Syamal<u></u><u></u></span></p>
</div>
</div>
<div>
<p class="MsoNormal" style="margin-bottom:12.0pt"><span style="font-family:"Calibri","sans-serif""><u></u> <u></u></span></p>
<div>
<p class="MsoNormal"><span style="font-family:"Calibri","sans-serif"">On Thu, Jun 20, 2013 at 6:46 AM, jeffrey shaw <<a href="mailto:jayusp@hotmail.com" target="_blank">jayusp@hotmail.com</a>> wrote:<u></u><u></u></span></p>
<div>
<div>
<p class="MsoNormal" style="margin-bottom:12.0pt"><span style="font-family:"Calibri","sans-serif"">Steve Reed says:<br>
"</span><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d">I am in Bangladesh…they think they have L donovani in dogs here</span><span style="font-family:"Calibri","sans-serif"">
</span><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d">Is that the case in China? Or elsewhere?"</span><span style="font-family:"Calibri","sans-serif""><br>
<br>
<br>
</span><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d">Does anybody know of a bone fide ID of donovani in dogs?</span><span style="font-family:"Calibri","sans-serif""><br>
<br>
<br>
</span><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d">Jeff</span><span style="font-family:"Calibri","sans-serif""><u></u><u></u></span></p>
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