<html><body><div style="color:#000; background-color:#fff; font-family:times new roman, new york, times, serif;font-size:12pt"><div id="yiv6911309742"><div><div style="color: rgb(0, 0, 0); background-color: rgb(255, 255, 255); font-family: 'times new roman', 'new york', times, serif; font-size: 12pt;"><div id="yiv6911309742yui_3_7_2_28_1371789117341_54"><span id="yiv6911309742yui_3_7_2_28_1371789117341_105">Dear All, </span></div><div id="yiv6911309742yui_3_7_2_28_1371789117341_54" style="color: rgb(0, 0, 0); font-size: 16px; font-family: 'times new roman', 'new york', times, serif; background-color: transparent; font-style: normal;"><span id="yiv6911309742yui_3_7_2_28_1371789117341_109">I agree with Katakura that if new technologies are applied new reservoirs can be found. In India it is taught that no animal reservoir exists. But we when we applied rK39 and PCR technologies we found a significant number of goats positive for anti-rK39
antibodies and by PCR. However, none of the 3 dogs tested was seropositive. Hence no finding can be ignored. </span></div><div id="yiv6911309742yui_3_7_2_28_1371789117341_54" style="color: rgb(0, 0, 0); font-size: 16px; font-family: 'times new roman', 'new york', times, serif; background-color: transparent; font-style: normal;" class="yui_3_7_2_28_1371789561939_100"><span>Please see attached publication on goat as reservoir of Leishmania dononovani in India. </span></div><div id="yiv6911309742yui_3_7_2_28_1371789117341_54" style="color: rgb(0, 0, 0); font-size: 16px; font-family: 'times new roman', 'new york', times, serif; background-color: transparent; font-style: normal;" class="yui_3_7_2_28_1371789561939_100"><span><br></span></div><div id="yiv6911309742yui_3_7_2_28_1371789117341_56"></div><div id="yiv6911309742yui_3_7_2_28_1371789117341_58"> </div><div id="yiv6911309742yui_3_7_2_28_1371789117341_60"><font color="#00407f"><b><i>Prof.
Sarman Singh, MD, FAMS, FIMSA, FRSTMH<br></i>Head, </b></font></div><div id="yiv6911309742yui_3_7_2_28_1371789117341_62"><font color="#00407f"><b>Division of Clinical Microbiology & Molecular Medicine,<br>All India Institute of Medical Sciences,
</b></font></div><div id="yiv6911309742yui_3_7_2_28_1371789117341_64"><font color="#00407f"><b>New Delhi (India)<br>Editor: Journal of Laboratory Physicians</b></font></div><div id="yiv6911309742yui_3_7_2_28_1371789117341_66"><font color="#00407f"><b>Phone: +91-11-26594977, 26588484<br>Fax: +91-11-26588663, 26588641<br></b></font></div><div><br id="yiv6911309742yui_3_7_2_28_1371789117341_68"><blockquote style="border-left:2px solid rgb(16, 16, 255);margin-left:5px;margin-top:5px;padding-left:5px;" id="yiv6911309742yui_3_7_2_28_1371789117341_71"> <div style="font-family: 'times new roman', 'new york', times, serif; font-size: 12pt;" class="yiv6911309742yui_3_7_2_28_1371789117341_74"> <div style="font-family: 'times new roman', 'new york', times, serif; font-size: 12pt;" class="yiv6911309742yui_3_7_2_28_1371789117341_75"> <div dir="ltr"> <hr size="1"> <font size="2" face="Arial"> <b><span style="font-weight:bold;">From:</span></b> Anuradha Dube
<anuradha_dube@hotmail.com><br> <b><span style="
font-weight:bold;">To:</span></b> Syamal Roy <sroy@iicb.res.in>; jeffrey shaw <jayusp@hotmail.com> <br><b><span style="font-weight:bold;">Cc:</span></b> "leish-l@lineu.icb.usp.br" <leish-l@lineu.icb.usp.br> <br> <b><span style="font-weight:bold;">Sent:</span></b> Thursday, June 20, 2013 10:24 PM<br> <b><span style="font-weight:bold;">Subject:</span></b> Re: [Leish-l] L.donovani in dogs?<br> </font> </div> <div class="yiv6911309742y_msg_container"><br><div id="yiv6911309742">
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<div><div dir="ltr">Dear Syamal<div><br></div><div>I have tried to develop dog as a model for L. donovani ( Dd8 strain) while I was working on monkey model in late nineties. I have infected stray dogs but none were found positive on day 30 p.i. </div><div><br></div><div>Anuradha</div><br><div><font face="Comic Sans MS">ANURADHA DUBE, Ph.D <br>Chief Scientist (Scientist G) ,<br>Leishmania laboratory, Division of Parasitology <br>Central Drug Research Institute <br>Chattar Manzil Palace <br>LUCKNOW-226001 Uttar Pradesh, INDIA <br>Ph nos: OFF 522 2612411-18 EXT: 4398 Fax no: 522 2623405 <br>RES: 2733454/4070667;mobile # 9450931332/9415104918; </font></div>
<div><font face="Comic Sans MS">e-mail: </font><a rel="nofollow" ymailto="mailto:anuradha_dube@hotmail.com; a_dube@cdri.res.in;" target="_blank" href="mailto:anuradha_dube@hotmail.com; a_dube@cdri.res.in;"><font face="Comic Sans MS">anuradha_dube@hotmail.com; anuradhadube@gmail.com; a_dube@cdri.res.in; </font></a> <br>------------------------------------------------------------<br></div><br><br><div><hr id="yiv6911309742stopSpelling">Date: Thu, 20 Jun 2013 08:35:37 +0530<br>From: sroy@iicb.res.in<br>To: jayusp@hotmail.com<br>CC: leish-l@lineu.icb.usp.br<br>Subject: Re: [Leish-l] L.donovani in dogs?<br><br><div dir="ltr"><div>Dear Steve</div><div>Dear Jeff:</div><div>The information seems interesting. I do not know any such information about VL in dogs in India. India and Bangladesh border is porous and I would assume that may be there are cases of VL infected dogs in India as well. How authentic this information is? Do they have
any data? May I have it?</div>
<div>Best</div><div>Syamal</div></div><div class="yiv6911309742ecxgmail_extra"><br><br><div class="yiv6911309742ecxgmail_quote">On Thu, Jun 20, 2013 at 6:46 AM, jeffrey shaw <span dir="ltr"><<a rel="nofollow" ymailto="mailto:jayusp@hotmail.com" target="_blank" href="mailto:jayusp@hotmail.com">jayusp@hotmail.com</a>></span> wrote:<br>
<blockquote class="yiv6911309742ecxgmail_quote" style="border-left:1px #ccc solid;padding-left:1ex;">
<div><div dir="ltr">Steve Reed says:<br>"<span style="color:rgb(31,73,125);font-size:11pt;">I am in Bangladesh…they think they have L donovani in dogs here</span>
<span style="color:rgb(31,73,125);font-size:11pt;">Is that the case in China? Or elsewhere?"</span><br><br><span style="color:rgb(31,73,125);font-size:11pt;"></span><br>
<span style="color:rgb(31,73,125);font-size:11pt;">Does anybody know of a bone fide ID of donovani in dogs?</span><br><br><span style="color:rgb(31,73,125);font-size:11pt;"></span><br>
<span style="color:rgb(31,73,125);font-size:11pt;">Jeff<br></span><br> </div><br><br>--<br>
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