<html><body><div style="color:#000; background-color:#fff; font-family:verdana, helvetica, sans-serif;font-size:10pt">even I didnot receive it<br><div><span></span></div><div> </div><div><i><font color="#0000bf">---------------------------------------------------------------</font></i></div><i>ska</i><div><br></div> <div style="font-family: verdana, helvetica, sans-serif; font-size: 10pt;"> <div style="font-family: times new roman, new york, times, serif; font-size: 12pt;"> <div dir="ltr"> <hr size="1"> <font face="Arial" size="2"> <b><span style="font-weight:bold;">From:</span></b> "McMahon-Pratt, Diane" <diane.mcmahon-pratt@yale.edu><br> <b><span style="font-weight: bold;">To:</span></b> Abed Nasereddin <abedn@ekmd.huji.ac.il>; neena goyal <neenacdri@yahoo.com>; AlbertDescoteaux <Albert.Descoteaux@iaf.inrs.ca>; Sunil Arora <skarora_in@yahoo.com> <br><b><span style="font-weight: bold;">Cc:</span></b> Leish-L email
post <Leish-l@lineu.icb.usp.br> <br> <b><span style="font-weight: bold;">Sent:</span></b> Monday, 22 April 2013 9:04 PM<br> <b><span style="font-weight: bold;">Subject:</span></b> RE: [Leish-l] Fwd: hot spots<br> </font> </div> <div class="y_msg_container"><br><div id="yiv8379995018">
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<div style="direction:ltr;font-family:Arial;color:#000000;font-size:12pt;">Hi-<br>
<br>
I did not receive the attachment. Could this be sent again?<br>
<br>
Thanks-<br>
<br>
Diane<br>
<div style="font-family:Times New Roman;color:#000000;font-size:16px;">
<hr tabindex="-1">
<div style="direction:ltr;" id="yiv8379995018divRpF246365"><font color="#000000" face="Tahoma" size="2"><b>From:</b> Abed Nasereddin [abedn@ekmd.huji.ac.il]<br>
<b>Sent:</b> Monday, April 22, 2013 2:15 AM<br>
<b>To:</b> neena goyal; AlbertDescoteaux; Sunil Arora; McMahon-Pratt, Diane<br>
<b>Cc:</b> Leish-L email post<br>
<b>Subject:</b> RE: [Leish-l] Fwd: hot spots<br>
</font><br>
</div>
<div></div>
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<div class="yiv8379995018WordSection1">
<div class="yiv8379995018MsoNormal"><span style="font-size:11.0pt;color:#1F497D;">Dear All,</span></div>
<div class="yiv8379995018MsoNormal"><span style="font-size:11.0pt;color:#1F497D;">See our attached published researh on axenic amastigote/ amastigote like characteriztion, Nasereddin et al. “Characterization of Leishmania (Leishmania)
tropica axenic amastigotes”.</span></div>
<div class="yiv8379995018MsoNormal"><span style="font-size:11.0pt;color:#1F497D;">Good luck</span></div>
<div class="yiv8379995018MsoNormal"><span style="font-size:11.0pt;color:#1F497D;">Abed</span></div>
<div class="yiv8379995018MsoNormal"><span style="font-size:11.0pt;color:#1F497D;"> </span></div>
<div class="yiv8379995018MsoNormal"><span style="font-size:11.0pt;color:#1F497D;"> </span></div>
<div style="border:none;border-top:solid #B5C4DF 1.0pt;padding:3.0pt 0in 0in 0in;">
<div class="yiv8379995018MsoNormal"><b><span style="font-size:10.0pt;">From:</span></b><span style="font-size:10.0pt;"> leish-l-bounces@lineu.icb.usp.br [mailto:leish-l-bounces@lineu.icb.usp.br]
<b>On Behalf Of </b>neena goyal<br>
<b>Sent:</b> Tuesday, April 09, 2013 9:03 AM<br>
<b>To:</b> AlbertDescoteaux; Sunil Arora; DianeMcMahon-Pratt<br>
<b>Cc:</b> Leish-L email post<br>
<b>Subject:</b> Re: [Leish-l] Fwd: hot spots</span></div>
</div>
<div class="yiv8379995018MsoNormal"> </div>
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<div class="yiv8379995018MsoNormal">Dear All, </div>
<div>
<div class="yiv8379995018MsoNormal">I feel this is the right time to define parameters to be used to characterize axenic amastigote/ amastigote like/stressed out promastigotes before start using them, as every one of us is worrying about
<u>the characterization</u> and many of us have an experience of working with these axenic amastigotes at various levels for almost more than one or two decades<br>
Neena<br>
--- On <b>Fri, 5/4/13, McMahon-Pratt, Diane <i><<a rel="nofollow" ymailto="mailto:diane.mcmahon-pratt@yale.edu" target="_blank" href="mailto:diane.mcmahon-pratt@yale.edu">diane.mcmahon-pratt@yale.edu</a>></i></b> wrote:</div>
<div class="yiv8379995018MsoNormal" style="margin-bottom:12.0pt;"><br>
From: McMahon-Pratt, Diane <<a rel="nofollow" ymailto="mailto:diane.mcmahon-pratt@yale.edu" target="_blank" href="mailto:diane.mcmahon-pratt@yale.edu">diane.mcmahon-pratt@yale.edu</a>><br>
Subject: Re: [Leish-l] Fwd: hot spots<br>
To: "Descoteaux, Albert" <<a rel="nofollow" ymailto="mailto:Albert.Descoteaux@iaf.inrs.ca" target="_blank" href="mailto:Albert.Descoteaux@iaf.inrs.ca">Albert.Descoteaux@iaf.inrs.ca</a>>, "Sunil Arora" <<a rel="nofollow" ymailto="mailto:skarora_in@yahoo.com" target="_blank" href="mailto:skarora_in@yahoo.com">skarora_in@yahoo.com</a>><br>
Cc: "Leish-L email post" <<a rel="nofollow" ymailto="mailto:Leish-l@lineu.icb.usp.br" target="_blank" href="mailto:Leish-l@lineu.icb.usp.br">Leish-l@lineu.icb.usp.br</a>><br>
Date: Friday, 5 April, 2013, 7:38 AM</div>
<div id="yiv8379995018">
<div>
<div>
<div class="yiv8379995018MsoNormal"><span style="color:black;">The real problem here is that people fail to characterize their organisms (axenic amastigotes). Some indeed are shocked promastigotes - others grow in culture and are amastigote-like
(close approximations and in many characteristics identical). Deflagellation does not mean amastigote. I have long felt people are sloppy in their characterization and hence we have a field where the "axenic amastigotes" connotation has variable meanings.
I think this should be changed. I would not eschew the use of axenic amastigotes for the failure of some. We all need to carefully characterize there and use them accordingly.
</span></div>
<div>
<div class="yiv8379995018MsoNormal" style="text-align:center;" align="center"><span style="color:black;">
<hr align="center" size="2" width="100%">
</span></div>
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<div class="yiv8379995018MsoNormal" style="margin-bottom:12.0pt;"><b><span style="font-size:10.0pt;color:black;">From:</span></b><span style="font-size:10.0pt;color:black;">
<a rel="nofollow" ymailto="mailto:leish-l-bounces@lineu.icb.usp.br" target="_blank" href="mailto:leish-l-bounces@lineu.icb.usp.br">leish-l-bounces@lineu.icb.usp.br</a> [leish-l-bounces@lineu.icb.usp.br] on behalf of Descoteaux, Albert [Albert.Descoteaux@iaf.inrs.ca]<br>
<b>Sent:</b> Friday, March 29, 2013 8:34 PM<br>
<b>To:</b> Sunil Arora<br>
<b>Cc:</b> Leish-L email post<br>
<b>Subject:</b> Re: [Leish-l] Fwd: hot spots</span><span style="color:black;"></span></div>
</div>
<div>
<div>
<div class="yiv8379995018MsoNormal"><span style="color:black;">Related to this issue, what are really the mammalian signals that induce promastigote-to-amastigote differentiation? </span></div>
</div>
<div>
<div class="yiv8379995018MsoNormal"><span style="color:black;"> </span></div>
</div>
<div>
<div class="yiv8379995018MsoNormal"><span style="color:black;">Albert<br>
<br>
Envoyé de mon iPad</span></div>
</div>
<div>
<div class="yiv8379995018MsoNormal" style="margin-bottom:12.0pt;"><span style="color:black;"><br>
Le 2013-03-29 à 15:58, "Sunil Arora" <<a href="" rel="nofollow">skarora_in@yahoo.com</a>> a écrit :</span></div>
</div>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt;">
<div>
<div>
<div>
<div class="yiv8379995018MsoNormal" style="background:white;"><span style="font-size:10.0pt;color:black;">I agree...axenic amastigotes are more like stressed out promastigotes... I always prefer working with something inside the macrophages
as a model for testing drugs or immunomodulators which I believe are closer to actual intracellular amastigotes in the infected host</span></div>
</div>
<div>
<div class="yiv8379995018MsoNormal"><span style="font-size:10.0pt;color:black;"> </span></div>
</div>
<div>
<div class="yiv8379995018MsoNormal"><span style="font-size:10.0pt;color:black;">best</span></div>
</div>
<div>
<div class="yiv8379995018MsoNormal"><span style="font-size:10.0pt;color:black;">sunil</span></div>
</div>
<div>
<div class="yiv8379995018MsoNormal" style="background:white;"><span style="font-size:10.0pt;color:black;"> </span></div>
</div>
<div>
<div class="yiv8379995018MsoNormal" style="background:white;"><i><span style="font-size:10.0pt;color:#0000BF;">---------------------------------------------------------------</span></i><span style="font-size:10.0pt;color:black;"></span></div>
</div>
<div>
<div class="yiv8379995018MsoNormal" style="background:white;"><i><span style="font-size:10.0pt;color:#0000BF;">Dr Sunil K.Arora<br>
Professor <br>
In Charge- HIV Diagnostic and Disease Monitoring Laboratory &<br>
NACO State Reference Laboratory,</span></i><span style="font-size:10.0pt;color:black;"></span></div>
</div>
<div>
<div class="yiv8379995018MsoNormal" style="background:white;"><span style="font-size:10.0pt;color:black;"> </span></div>
</div>
<div>
<div class="yiv8379995018MsoNormal" style="background:white;"><i><span style="font-size:10.0pt;color:#0000BF;">Councillor- Federation of Immunological Societies of Asia-oceania (FIMSA)</span></i><span style="font-size:10.0pt;color:black;"></span></div>
</div>
<div>
<div class="yiv8379995018MsoNormal"><i><span style="font-size:10.0pt;color:#0000BF;">Vice President- Indian Immunology Society (IIS)</span></i><span style="font-size:10.0pt;color:#0000BF;"></span></div>
</div>
<div>
<div class="yiv8379995018MsoNormal"><i><span style="font-size:10.0pt;color:#0000BF;">Secretary (Research)- The Cytometry Society of India (TCS)</span></i><span style="font-size:10.0pt;color:#0000BF;"></span></div>
</div>
<div>
<div class="yiv8379995018MsoNormal"><span style="font-size:10.0pt;color:#0000BF;"> </span></div>
</div>
<div>
<div class="yiv8379995018MsoNormal"><i><span style="font-size:10.0pt;color:#0000BF;">Department of Immunopathology<br>
PGIMER, Chandigarh-160 012<br>
Ph.: 0091-172-2755192 (Off)<br>
FAX: 0091-172-2744401, 2745078<br>
Ph:+91-172-4666087 (Res); 9872866087 (cell)<br>
email: <a href="" rel="nofollow">skarora_in@yahoo.com</a> ; <a href="" rel="nofollow">
skarorain@gmail.com</a> </span></i><span style="font-size:10.0pt;color:#0000BF;"></span></div>
</div>
<div>
<div class="yiv8379995018MsoNormal" style="background:white;"><span style="font-size:10.0pt;color:black;"> </span></div>
</div>
<div>
<div>
<div>
<div class="yiv8379995018MsoNormal" style="text-align:center;background:white;" align="center">
<span style="font-size:10.0pt;color:black;">
<hr align="center" size="1" width="100%">
</span></div>
<div class="yiv8379995018MsoNormal" style="background:white;"><b><span style="font-size:10.0pt;color:black;">From:</span></b><span style="font-size:10.0pt;color:black;"> Clos Joachim <<a href="" rel="nofollow">clos@bni-hamburg.de</a>><br>
<b>To:</b> Leish-L email post <<a href="" rel="nofollow">Leish-l@lineu.icb.usp.br</a>>
<br>
<b>Sent:</b> Monday, 11 March 2013 8:16 PM<br>
<b>Subject:</b> [Leish-l] Fwd: hot spots</span><span style="color:black;"></span></div>
</div>
<div class="yiv8379995018MsoNormal" style="background:white;"><span style="color:black;"> </span></div>
<div id="yiv8379995018">
<div>
<div>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt;">
<div>
<div class="yiv8379995018MsoNormal" style="background:white;"><span style="font-size:10.0pt;color:#010101;">Are axenic amastigotes a valid model (and for what?), the first developmental step towards real amastigotes, or just stressed-out promastigotes? Many
of us use axenics and swear by them, others are skeptical, others will accept what's inside a macrophage, still others only want amastigotes isolated from something with a fur. I believe we should come to some sort of common language and interpretation. I
am somewhere in the middle myself, preferring to do things in macrophages whenever possible.</span><span style="color:black;">
</span></div>
<div>
<div class="yiv8379995018MsoNormal" style="background:white;"><span style="font-size:10.0pt;color:#010101;">Cheers!</span><span style="color:black;"></span></div>
</div>
</div>
</blockquote>
</div>
<div>
<div class="yiv8379995018MsoNormal" style="background:white;"><span style="color:black;"><br>
<br>
</span></div>
<div>
<div>
<div class="yiv8379995018MsoNormal" style="background:white;"><span style="color:black;"> </span></div>
</div>
<div>
<div class="yiv8379995018MsoNormal" style="background:white;"><span style="color:black;"> </span></div>
<div>
<div>
<div class="yiv8379995018MsoNormal" style="background:white;"><span style="color:black;">Am 07.02.2013 um 20:38 schrieb Carlos Costa:</span></div>
</div>
<div class="yiv8379995018MsoNormal" style="background:white;"><span style="color:black;"><br>
<br>
</span></div>
<div>
<div class="yiv8379995018MsoNormal" style="background:white;"><span style="color:black;">What are the most polemic issues in leishmaniasis?<br clear="all">
</span><span style="color:black;"></span></div>
<div>
<div class="yiv8379995018MsoNormal" style="background:white;"><span style="color:black;"> </span></div>
</div>
<div class="yiv8379995018MsoNormal" style="background:white;"><span style="color:black;">-- </span>
</div>
<div class="yiv8379995018MsoNormal" style="background:white;"><span style="color:black;">Carlos H. N. Costa, MD, DSc.</span><span style="color:black;"></span></div>
<div class="yiv8379995018MsoNormal" style="background:white;"><span style="color:black;">Sociedade Brasileira de Medicina Tropical </span><span style="color:black;"></span></div>
<div class="yiv8379995018MsoNormal" style="background:white;"><span style="color:black;">(Brazilian Society of Tropical Medicine)</span><span style="color:black;"></span></div>
<div class="yiv8379995018MsoNormal" style="background:white;"><span style="color:black;">President</span><span style="color:black;"></span></div>
<div class="yiv8379995018MsoNormal" style="background:white;"><span style="color:black;"> </span></div>
<div class="yiv8379995018MsoNormal" style="background:white;"><span style="color:black;">Universidade Federal do Piauí</span><span style="color:black;"></span></div>
<div>
<div class="yiv8379995018MsoNormal" style="background:white;"><span style="color:black;">Instituto de Doenças Tropicais Natan Portella</span><span style="color:black;"></span></div>
<div class="yiv8379995018MsoNormal" style="background:white;"><span style="color:black;">Rua Artur de Vasconcelos 151-Sul</span><span style="color:black;"></span></div>
<div>
<div class="yiv8379995018MsoNormal" style="background:white;"><span style="color:black;">64001-450 Teresina-PI</span><span style="color:black;"></span></div>
<div class="yiv8379995018MsoNormal" style="background:white;"><span style="color:black;">Brazil</span></div>
<div class="yiv8379995018MsoNormal" style="background:white;"><span style="color:black;">Telephones: +55 86 3222-4377 (W),
</span></div>
<div class="yiv8379995018MsoNormal" style="text-align:center;background:white;" align="center"><span style="color:black;">+55 86 3221-3062 (W), </span></div>
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