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<div style="direction: ltr;font-family: Arial;color: #000000;font-size: 12pt;">The real problem here is that people fail to characterize their organisms (axenic amastigotes). Some indeed are shocked promastigotes - others grow in culture and are amastigote-like
(close approximations and in many characteristics identical). Deflagellation does not mean amastigote. I have long felt people are sloppy in their characterization and hence we have a field where the "axenic amastigotes" connotation has variable meanings.
I think this should be changed. I would not eschew the use of axenic amastigotes for the failure of some. We all need to carefully characterize there and use them accordingly.
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<div style="direction: ltr;" id="divRpF189581"><font color="#000000" face="Tahoma" size="2"><b>From:</b> leish-l-bounces@lineu.icb.usp.br [leish-l-bounces@lineu.icb.usp.br] on behalf of Descoteaux, Albert [Albert.Descoteaux@iaf.inrs.ca]<br>
<b>Sent:</b> Friday, March 29, 2013 8:34 PM<br>
<b>To:</b> Sunil Arora<br>
<b>Cc:</b> Leish-L email post<br>
<b>Subject:</b> Re: [Leish-l] Fwd: hot spots<br>
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<div>Related to this issue, what are really the mammalian signals that induce promastigote-to-amastigote differentiation? </div>
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<div>Albert<br>
<br>
Envoyé de mon iPad</div>
<div><br>
Le 2013-03-29 à 15:58, "Sunil Arora" <<a href="mailto:skarora_in@yahoo.com" target="_blank">skarora_in@yahoo.com</a>> a écrit :<br>
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<div><span>I agree...axenic amastigotes are more like stressed out promastigotes... I always prefer working with something inside the macrophages as a model for testing drugs or immunomodulators which I believe are closer to actual intracellular amastigotes
in the infected host</span></div>
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<div style="color:rgb(0,0,0); font-size:13px; font-family:verdana,helvetica,sans-serif; background-color:transparent; font-style:normal">
<span>best</span></div>
<div style="color:rgb(0,0,0); font-size:13px; font-family:verdana,helvetica,sans-serif; background-color:transparent; font-style:normal">
<span>sunil</span></div>
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<div> </div>
<div><i><font color="#0000bf">---------------------------------------------------------------</font></i></div>
<div><i><font color="#0000bf">Dr Sunil K.Arora<br>
Professor <br>
In Charge- HIV Diagnostic and Disease Monitoring Laboratory &<br>
NACO State Reference Laboratory,</font></i></div>
<div><i><font color="#0000bf"></font></i> </div>
<div><i><font color="#0000bf">Councillor- Federation of Immunological Societies of Asia-oceania (FIMSA)</font></i></div>
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<i><font color="#0000bf"><var id="yui-ie-cursor"></var>Department of Immunopathology<br>
PGIMER, Chandigarh-160 012<br>
Ph.: 0091-172-2755192 (Off)<br>
FAX: 0091-172-2744401, 2745078<br>
Ph:+91-172-4666087 (Res); 9872866087 (cell)<br>
</font><font color="#0000bf">email: <a rel="nofollow" href="mailto:skarora_in@yahoo.com" target="_blank">
skarora_in@yahoo.com</a> ; <a rel="nofollow" href="mailto:skarorain@gmail.com" target="_blank">
skarorain@gmail.com</a> </font></i></div>
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<b><span style="font-weight:bold">From:</span></b> Clos Joachim <<a href="mailto:clos@bni-hamburg.de" target="_blank">clos@bni-hamburg.de</a>><br>
<b><span style="font-weight:bold">To:</span></b> Leish-L email post <<a href="mailto:Leish-l@lineu.icb.usp.br" target="_blank">Leish-l@lineu.icb.usp.br</a>>
<br>
<b><span style="font-weight:bold">Sent:</span></b> Monday, 11 March 2013 8:16 PM<br>
<b><span style="font-weight:bold">Subject:</span></b> [Leish-l] Fwd: hot spots<br>
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<div style="word-wrap:break-word"><font class="yiv1658093374Apple-style-span" style="font-size:13px" color="#010101" face="Baghdad">Are axenic amastigotes a valid model (and for what?), the first developmental step towards real amastigotes, or just stressed-out
promastigotes? Many of us use axenics and swear by them, others are skeptical, others will accept what's inside a macrophage, still others only want amastigotes isolated from something with a fur. I believe we should come to some sort of common language and
interpretation. I am somewhere in the middle myself, preferring to do things in macrophages whenever possible.</font>
<div><font class="yiv1658093374Apple-style-span" style="font-size:13px" color="#010101" face="Baghdad">Cheers!</font></div>
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<div>Am 07.02.2013 um 20:38 schrieb Carlos Costa:</div>
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<div dir="ltr"><font color="#000000"><font><font face="verdana,sans-serif">What are the most polemic issues in leishmaniasis?<br clear="all">
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<div style="text-align:left"><span style="font-family:verdana,sans-serif">Sociedade Brasileira de Medicina Tropical </span></div>
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