<html><body><div style="color:#000; background-color:#fff; font-family:verdana, helvetica, sans-serif;font-size:10pt"><div><span>I agree...axenic amastigotes are more like stressed out promastigotes... I always prefer working with something inside the macrophages as a model for testing drugs or immunomodulators which I believe are closer to actual intracellular amastigotes in the infected host</span></div><div style="color: rgb(0, 0, 0); font-size: 13px; font-family: verdana, helvetica, sans-serif; background-color: transparent; font-style: normal; "><span><br></span></div><div style="color: rgb(0, 0, 0); font-size: 13px; font-family: verdana, helvetica, sans-serif; background-color: transparent; font-style: normal; "><span>best</span></div><div style="color: rgb(0, 0, 0); font-size: 13px; font-family: verdana, helvetica, sans-serif; background-color: transparent; font-style: normal; "><span>sunil</span></div><div></div><div> </div><div><i><font
color="#0000bf">---------------------------------------------------------------</font></i></div>
<div><i><font color="#0000bf">Dr Sunil K.Arora<br>Professor <br>In Charge- HIV Diagnostic and Disease Monitoring Laboratory &<br>NACO State Reference Laboratory,</font></i></div>
<div><i><font color="#0000bf"></font></i> </div>
<div><i><font color="#0000bf">Councillor- Federation of Immunological Societies of Asia-oceania (FIMSA)</font></i></div>
<div style="background-color: transparent; font-style: normal; font-family: arial, helvetica, clean, sans-serif; color: rgb(0, 0, 191); font-size: 13px; "><i>Vice President- Indian Immunology Society (IIS)</i></div>
<div style="background-color: transparent; font-style: normal; font-family: arial, helvetica, clean, sans-serif; color: rgb(0, 0, 191); font-size: 13px; "><i>Secretary (Research)- The Cytometry Society of India (TCS)</i></div>
<div style="background-color: transparent; font-style: normal; font-family: arial, helvetica, clean, sans-serif; color: rgb(0, 0, 191); font-size: 13px; "><i><font color="#0000bf"></font></i> </div>
<div style="background-color: transparent; font-style: normal; font-family: arial, helvetica, clean, sans-serif; color: rgb(0, 0, 191); font-size: 13px; "><i><font color="#0000bf"><var id="yui-ie-cursor"></var>Department of Immunopathology<br>PGIMER, Chandigarh-160 012<br>Ph.: 0091-172-2755192 (Off)<br>FAX: 0091-172-2744401, 2745078<br>Ph:+91-172-4666087 (Res); 9872866087 (cell)<br></font><font color="#0000bf">email: <a rel="nofollow" target="_blank" href="mailto:skarora_in@yahoo.com">skarora_in@yahoo.com</a> ; <a rel="nofollow" target="_blank" href="mailto:skarorain@gmail.com">skarorain@gmail.com</a> </font></i></div><div><br></div> <div style="font-family: verdana, helvetica, sans-serif; font-size: 10pt; "> <div style="font-family: 'times new roman', 'new york', times, serif; font-size: 12pt; "> <div dir="ltr"> <font size="2" face="Arial"> <hr size="1"> <b><span style="font-weight:bold;">From:</span></b> Clos Joachim <clos@bni-hamburg.de><br>
<b><span style="font-weight: bold;">To:</span></b> Leish-L email post <Leish-l@lineu.icb.usp.br> <br> <b><span style="font-weight: bold;">Sent:</span></b> Monday, 11 March 2013 8:16 PM<br> <b><span style="font-weight: bold;">Subject:</span></b> [Leish-l] Fwd: hot spots<br> </font> </div> <br>
<div id="yiv1658093374"><div><div><blockquote type="cite"><div style="word-wrap:break-word;"><font class="yiv1658093374Apple-style-span" color="#010101" face="Baghdad" style="font-size:13px;">Are axenic amastigotes a valid model (and for what?), the first developmental step towards real amastigotes, or just stressed-out promastigotes? Many of us use axenics and swear by them, others are skeptical, others will accept what's inside a macrophage, still others only want amastigotes isolated from something with a fur. I believe we should come to some sort of common language and interpretation. I am somewhere in the middle myself, preferring to do things in macrophages whenever possible.</font><div><font class="yiv1658093374Apple-style-span" color="#010101" face="Baghdad" style="font-size:13px;">Cheers!</font></div></div></blockquote></div><div><br><blockquote type="cite"><div style="word-wrap:break-word;"><div><br></div><div><br><div><div>Am 07.02.2013
um 20:38 schrieb Carlos Costa:</div><br class="yiv1658093374Apple-interchange-newline"><blockquote type="cite"><div dir="ltr"><font color="#000000"><font><font face="verdana,sans-serif">What are the most polemic issues in leishmaniasis?<br clear="all"></font></font></font><div><br></div>-- <br><div style="text-align:left;"><span style="font-family: verdana, sans-serif; ">Carlos H. N. Costa, MD, DSc.</span></div>
<div style="text-align:left;"><span style="font-family: verdana, sans-serif; ">Sociedade Brasileira de Medicina Tropical </span></div><div style="text-align:left;"><span style="font-family: verdana, sans-serif; "></span><span style="font-family: verdana, sans-serif; ">(Brazilian Society of Tropical Medicine)</span></div>
<div style="text-align:left;"><span style="font-family: verdana, sans-serif; ">President</span></div><div style="text-align:left;"><span style="font-family: verdana, sans-serif; "><br></span></div><div style="text-align:left;"><span style="font-family: verdana, sans-serif; ">Universidade Federal do Piauí</span></div>
<div><div style="text-align:left;"><span style="font-family: verdana, sans-serif; ">Instituto de Doenças Tropicais Natan Portella</span></div><div style="text-align:left;"><font face="verdana, sans-serif">Rua Artur de Vasconcelos 151-Sul</font></div>
<div><div style="text-align:left;"><span style="font-family: verdana, sans-serif; ">64001-450 Teresina-PI</span></div><font face="verdana, sans-serif"><div style="text-align:left;">Brazil</div><div style="text-align:left;">Telephones: +55 86 3222-4377 (W), <div style="text-align:center;display:inline!important;">
<div style="text-align:center;display:inline!important;">+55 86 3221-3062 (W), </div></div><div style="text-align:center;display:inline!important;">+55 86 3237-1075 (R). </div></div><div style="text-align:left;"><br></div><div style="text-align:left;">
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class="yiv1658093374Apple-style-span" style="font-family: Helvetica; ">Bernhard Nocht St. 74, D-20359 Hamburg, FRG</span></font></div><div style="margin-top:0px;margin-right:0px;margin-bottom:0px;margin-left:0px;"><font size="3" style="font-style: normal; font-variant: normal; font-weight: normal; font-size: 12px; line-height: normal; font-family: Helvetica; "><span class="yiv1658093374Apple-style-span" style="font-family: Helvetica; ">Phone +49 40 42818-481; Fax -512</span></font></div><div style="margin-top:0px;margin-right:0px;margin-bottom:0px;margin-left:0px;"><font class="yiv1658093374Apple-style-span" face="Helvetica"><span class="yiv1658093374Apple-style-span" style="font-family: Helvetica; ">Skype: joachim.clos</span></font></div><br class="yiv1658093374Apple-interchange-newline"></span>
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