<html><head></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><div><blockquote type="cite"><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><font class="Apple-style-span" color="#010101" face="Baghdad" style="font-size: 13px;">Are axenic amastigotes a valid model (and for what?), the first developmental step towards real amastigotes, or just stressed-out promastigotes? Many of us use axenics and swear by them, others are skeptical, others will accept what's inside a macrophage, still others only want amastigotes isolated from something with a fur. I believe we should come to some sort of common language and interpretation. I am somewhere in the middle myself, preferring to do things in macrophages whenever possible.</font><div><font class="Apple-style-span" color="#010101" face="Baghdad" style="font-size: 13px;">Cheers!</font></div></div></blockquote></div><div><br><blockquote type="cite"><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><div><br></div><div><br><div><div>Am 07.02.2013 um 20:38 schrieb Carlos Costa:</div><br class="Apple-interchange-newline"><blockquote type="cite"><div dir="ltr"><font color="#000000"><font><font face="verdana,sans-serif">What are the most polemic issues in leishmaniasis?<br clear="all"></font></font></font><div><br></div>-- <br><div style="text-align:left"><span style="font-family:verdana,sans-serif">Carlos H. N. Costa, MD, DSc.</span></div>
<div style="text-align:left"><span style="font-family:verdana,sans-serif">Sociedade Brasileira de Medicina Tropical </span></div><div style="text-align:left"><span style="font-family:verdana,sans-serif"></span><span style="font-family:verdana,sans-serif">(Brazilian Society of Tropical Medicine)</span></div>
<div style="text-align:left"><span style="font-family:verdana,sans-serif">President</span></div><div style="text-align:left"><span style="font-family:verdana,sans-serif"><br></span></div><div style="text-align:left"><span style="font-family:verdana,sans-serif">Universidade Federal do Piauí</span></div>
<div><div style="text-align:left"><span style="font-family:verdana,sans-serif">Instituto de Doenças Tropicais Natan Portella</span></div><div style="text-align:left"><font face="verdana, sans-serif">Rua Artur de Vasconcelos 151-Sul</font></div>
<div><div style="text-align:left"><span style="font-family:verdana,sans-serif">64001-450 Teresina-PI</span></div><font face="verdana, sans-serif"><div style="text-align:left">Brazil</div><div style="text-align:left">Telephones: +55 86 3222-4377 (W), <div style="text-align:center;display:inline!important">
<div style="text-align:center;display:inline!important">+55 86 3221-3062 (W), </div></div><div style="text-align:center;display:inline!important">+55 86 3237-1075 (R). </div></div><div style="text-align:left"><br></div><div style="text-align:left">
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<span class="Apple-style-span" style="border-collapse: separate; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; font-family: Arial; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; -webkit-text-decorations-in-effect: none; text-indent: 0px; -webkit-text-size-adjust: auto; text-transform: none; orphans: 2; white-space: normal; widows: 2; word-spacing: 0px; "><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><font face="Helvetica" size="3" style="font: 12.0px Helvetica; font-family: Helvetica; "><span class="Apple-style-span" style="font-family: Helvetica; ">Joachim Clos, Dr. rer. nat.</span></font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><font face="Helvetica" size="3" style="font: 12.0px Helvetica; font-family: Helvetica; "><span class="Apple-style-span" style="font-family: Helvetica; ">Senior Staff Scientist</span></font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><font class="Apple-style-span" face="Helvetica"><span class="Apple-style-span" style="font-family: Helvetica; ">Group Leader</span></font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><font face="Helvetica" size="3" style="font: 12.0px Helvetica; font-family: Helvetica; "><span class="Apple-style-span" style="font-family: Helvetica; ">Bernhard Nocht Institute for Tropical Medicine</span></font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><font face="Helvetica" size="3" style="font: 12.0px Helvetica; font-family: Helvetica; "><span class="Apple-style-span" style="font-family: Helvetica; ">Bernhard Nocht St. 74, D-20359 Hamburg, FRG</span></font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><font face="Helvetica" size="3" style="font: 12.0px Helvetica; font-family: Helvetica; "><span class="Apple-style-span" style="font-family: Helvetica; ">Phone +49 40 42818-481; Fax -512</span></font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><font class="Apple-style-span" face="Helvetica"><span class="Apple-style-span" style="font-family: Helvetica; ">Skype: joachim.clos</span></font></div><br class="Apple-interchange-newline"></span>
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