<font color="#000000"><font><font face="verdana,sans-serif">Dear Jorge,</font></font></font><div><font color="#000000"><font><font face="verdana,sans-serif"><br></font></font></font></div><div><font color="#000000"><font><font face="verdana,sans-serif">Thanks for your interesting observation. I just called Ivete Mendonça asking about your point of adherence to gut wall. She says promastigotes were not adhered to the hindgut. So, possibly you hit the point. After I mentioned your hypothesis she thinks it is plausible, since she clearly see them adhered to the midgut but never to the hindgut and rectal ampulla.</font></font></font></div>
<div><font color="#000000"><font><font face="verdana,sans-serif"><br></font></font></font></div><div><font color="#000000"><font><font face="verdana,sans-serif">This is in accordance to the observations that kDNA of Leishmania from dogs in Teresina and from two sand flies fed on dogs all were L. infantum (see Alonso et al, 2012, attached).</font></font></font></div>
<div><font color="#000000"><font><font face="verdana,sans-serif"> </font></font></font></div><div><font color="#000000"><font><font face="verdana,sans-serif">Any way, it may be wiser to go through more samples from sandflies to confirm this hypothesis.</font></font></font></div>
<div><font color="#000000"><font><font face="verdana,sans-serif"><br></font></font></font></div><div><font color="#000000"><font><font face="verdana,sans-serif">Cheers,</font></font></font></div><div><font color="#000000"><font><font face="verdana,sans-serif"><br>
</font></font></font></div><div><font color="#000000"><font><font face="verdana,sans-serif">Carlos. <br></font></font></font><br><div class="gmail_quote">2012/6/13 Arias, Jorge Dr. <span dir="ltr"><<a href="mailto:Jorge.Arias@fairfaxcounty.gov" target="_blank">Jorge.Arias@fairfaxcounty.gov</a>></span><br>
<blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">Are they attached to the gut wall? If not, it may be due to presure while dissecting the gut out.<br>
<br>
Best wishes,<br>
<br>
<br>
<br>
Jorge Arias<br>
<br>
<br>
<br>
________________________________<br>
From: <a href="mailto:leish-l-bounces@lineu.icb.usp.br">leish-l-bounces@lineu.icb.usp.br</a> [<a href="mailto:leish-l-bounces@lineu.icb.usp.br">leish-l-bounces@lineu.icb.usp.br</a>] on behalf of Carlos Brisola Marcondes [<a href="mailto:cbrisolamarcondes@gmail.com">cbrisolamarcondes@gmail.com</a>]<br>
Sent: Monday, June 11, 2012 7:52 AM<br>
To: Carlos Costa<br>
Cc: Leish-L; Regiane Araujo<br>
Subject: Re: [Leish-l] L. infantum in the hindgut?<br>
<div class="im"><br>
Dear all,<br>
maybe there is an influence of carbohydrates in the localization of promastigotes in the gut, as observed by Añez et al. some years ago.<br>
<br>
Sincerely<br>
Carlos Brisola Marcondes<br>
<br>
</div>2012/6/2 Carlos Costa <<a href="mailto:chncosta@gmail.com">chncosta@gmail.com</a><mailto:<a href="mailto:chncosta@gmail.com">chncosta@gmail.com</a>>><br>
<div class="im">Dear all,<br>
<br>
Dr. Ivete Mendonça, in Teresina, Brazil, has consistently and systematically been observing promastigotes xenodiagnosis performed in dogs in the hindgut and rectal ampulla of Lutzomyia longipalpis. We are not sure if they are really L. infantum or Viannia. Does anybody has observed the same thing?<br>
<br>
By the way, any suggestions of primers to discriminate Leishmania from Viannia?<br>
<br>
Many thanks,<br>
<br>
Carlos.<br>
<br>
<br>
<br>
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</blockquote></div><br><br clear="all"><div><br></div>-- <br><div style="text-align:left"><span style="font-family:verdana,sans-serif">Carlos H. N. Costa, MD, DSc.</span></div><div style="text-align:left"><span style="font-family:verdana,sans-serif">Sociedade Brasileira de Medicina Tropical </span></div>
<div style="text-align:left"><span style="font-family:verdana,sans-serif"></span><span style="font-family:verdana,sans-serif">(Brazilian Society of Tropical Medicine)</span></div><div style="text-align:left"><span style="font-family:verdana,sans-serif">President</span></div>
<div style="text-align:left"><span style="font-family:verdana,sans-serif"><br></span></div><div style="text-align:left"><span style="font-family:verdana,sans-serif">Universidade Federal do Piauí</span></div><div><div style="text-align:left">
<span style="font-family:verdana,sans-serif">Instituto de Doenças Tropicais Natan Portella</span></div><div style="text-align:left"><font face="verdana, sans-serif">Rua Artur de Vasconcelos 151-Sul</font></div><div><div style="text-align:left">
<span style="font-family:verdana,sans-serif">64001-450 Teresina-PI</span></div><font face="verdana, sans-serif"><div style="text-align:left">Brazil</div><div style="text-align:left">Telephones: +55 86 3222-4377 (W), <div style="text-align:center;display:inline!important">
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