<html><head></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">SL RNA gene primers will also discriminate the subgenus Viannia from both cutaneous and visceral groups of subgenus Leishmania. <div><br><div>See: <span class="Apple-tab-span" style="white-space:pre"> </span>Fernandes et al., (1994) Mol. Biochem. Parasitol. 66:261-271.</div><div><span class="Apple-tab-span" style="white-space:pre"> </span>Harris et al., (1998) J. Clin. Micro. 36:1989-1995</div><div><br><div>Happy choosing between primer sets,</div><div><br></div><div>David</div><div><br><div><div>On Jun 10, 2012, at 4:01 PM, Carlos Costa wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite"><font color="#000000"><font><font face="verdana,sans-serif">Dear friends,</font></font></font><div><font color="#000000"><font><font face="verdana,sans-serif"><br></font></font></font></div><div><font color="#000000"><font><font face="verdana,sans-serif">Thanks for all the hints for the primers to discriminate Leishmania from Viannia. We will try some of them and check if the promastigotes Dr. Mendonça and our group have found in the hindgut of dog fed Lu. longipalpis correspond to L. infantum.</font></font></font></div>
<div><font color="#000000"><font><font face="verdana,sans-serif"><br></font></font></font></div><div><font color="#000000"><font><font face="verdana,sans-serif">Best wishes,</font></font></font></div><div><font color="#000000"><font><font face="verdana,sans-serif"><br>
</font></font></font></div><div><font color="#000000"><font><font face="verdana,sans-serif">Carlos.<br></font></font></font><br><div class="gmail_quote">2012/6/9 Chang, Kwang-Poo <span dir="ltr"><<a href="mailto:KwangPoo.Chang@rosalindfranklin.edu" target="_blank">KwangPoo.Chang@rosalindfranklin.edu</a>></span><br>
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<div><p class="MsoNormal"><font color="navy" face="Arial"><span style="font-size:10.0pt;font-family:Arial;color:navy">Nagt sequence analysis can easily differentiate
Viannia from Leishmania. You will find this in our publication in 2007 that
has been verified by additional data generated by <u></u>Carlos
Muskus<u></u>, <u></u><u></u>Medellin<u></u><u></u>.<u></u><u></u></span></font></p><p class="MsoNormal"><font color="navy" face="Arial"><span style="font-size:10.0pt;font-family:Arial;color:navy"><u></u> <u></u></span></font></p><p class="MsoNormal"><font color="navy" face="Arial"><span style="font-size:10.0pt;font-family:Arial;color:navy">Genes encoding protein elements for the
RNAi machinery are expected to exit only in the Viannia group, but I have not
looked into this by designing primers for the ease of their PCR amplification versus
nagt. These genes are expected to be absent in the Leishmania subgenus,
producing no PCR products. Such negative findings however may not be as good
as something positive in both groups, but different between them. <u></u><u></u></span></font></p><p class="MsoNormal"><font color="navy" face="Arial"><span style="font-size:10.0pt;font-family:Arial;color:navy"><u></u> <u></u></span></font></p><p class="MsoNormal"><font color="navy" face="Arial"><span style="font-size:10.0pt;font-family:Arial;color:navy">KP<u></u><u></u></span></font></p><p class="MsoNormal"><font color="navy" face="Arial"><span style="font-size:10.0pt;font-family:Arial;color:navy"><u></u> <u></u></span></font></p>
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</span></font></div><p class="MsoNormal"><b><font face="Tahoma"><span style="font-size:10.0pt;font-family:Tahoma;font-weight:bold">From:</span></font></b><font face="Tahoma"><span style="font-size:10.0pt;font-family:Tahoma">
<a href="mailto:leish-l-bounces@lineu.icb.usp.br" target="_blank">leish-l-bounces@lineu.icb.usp.br</a> [mailto:<a href="mailto:leish-l-bounces@lineu.icb.usp.br" target="_blank">leish-l-bounces@lineu.icb.usp.br</a>] <b><span style="font-weight:bold">On Behalf Of </span></b>Carlos Costa<br>
<b><span style="font-weight:bold">Sent:</span></b> Saturday, June 02, 2012 5:28
PM<br>
<b><span style="font-weight:bold">To:</span></b> Leish-L; Regiane Araujo; IVETE
LOPES DE MENDONCA</span></font></p><div class="im"><font face="Tahoma"><br>
<b><span style="font-weight:bold">Subject:</span></b> [Leish-l] L. infantum in
the hindgut?</font></div><u></u><u></u><div><br class="webkit-block-placeholder"></div>
</div><p class="MsoNormal"><font size="3" face="Times New Roman"><span style="font-size:12.0pt"><u></u> <u></u></span></font></p><p class="MsoNormal"><font size="3" color="black" face="Verdana"><span style="font-size:12.0pt;font-family:Verdana">Dear all,</span></font><u></u><u></u></p><div><div class="h5">
<div><p class="MsoNormal"><font size="3" face="Times New Roman"><span style="font-size:12.0pt"><u></u> <u></u></span></font></p>
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<div><p class="MsoNormal"><font size="3" color="black" face="Verdana"><span style="font-size:12.0pt;font-family:Verdana">Dr. Ivete Mendonça, in
<u></u><u></u>Teresina<u></u>, <u></u>Brazil<u></u><u></u>, has consistently and
systematically been observing promastigotes xenodiagnosis performed in dogs in
the hindgut and rectal ampulla of Lutzomyia longipalpis. We are not sure if
they are really L. infantum or Viannia. Does anybody has observed the same
thing?</span></font><u></u><u></u></p>
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<div><p class="MsoNormal"><font size="3" face="Times New Roman"><span style="font-size:12.0pt"><u></u> <u></u></span></font></p>
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<div><p class="MsoNormal"><font size="3" color="black" face="Verdana"><span style="font-size:12.0pt;font-family:Verdana">By the way, any
suggestions of primers to discriminate Leishmania from Viannia?</span></font><u></u><u></u></p>
</div>
<div><p class="MsoNormal"><font size="3" face="Times New Roman"><span style="font-size:12.0pt"><u></u> <u></u></span></font></p>
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<div><p class="MsoNormal"><font size="3" color="black" face="Verdana"><span style="font-size:12.0pt;font-family:Verdana">Many thanks,</span></font><u></u><u></u></p>
</div>
<div><p class="MsoNormal"><font size="3" face="Times New Roman"><span style="font-size:12.0pt"><u></u> <u></u></span></font></p>
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<div><p class="MsoNormal"><font size="3" color="black" face="Verdana"><span style="font-size:12.0pt;font-family:Verdana">Carlos.<br clear="all">
</span></font><u></u><u></u></p>
<div><p class="MsoNormal"><font size="3" face="Times New Roman"><span style="font-size:12.0pt"><u></u> <u></u></span></font></p>
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<div><p class="MsoNormal"><font face="Verdana"><span style="font-size:10.0pt;font-family:Verdana"><u></u> <u></u></span></font></p>
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</blockquote></div><br><br clear="all"><div><br></div>-- <br><div style="text-align:left"><span style="font-family:verdana,sans-serif">Carlos H. N. Costa, MD, DSc.</span></div><div style="text-align:left"><span style="font-family:verdana,sans-serif">Sociedade Brasileira de Medicina Tropical </span></div>
<div style="text-align:left"><span style="font-family:verdana,sans-serif"></span><span style="font-family:verdana,sans-serif">(Brazilian Society of Tropical Medicine)</span></div><div style="text-align:left"><span style="font-family:verdana,sans-serif">President</span></div>
<div style="text-align:left"><span style="font-family:verdana,sans-serif"><br></span></div><div style="text-align:left"><span style="font-family:verdana,sans-serif">Universidade Federal do Piauí</span></div><div><div style="text-align:left">
<span style="font-family:verdana,sans-serif">Instituto de Doenças Tropicais Natan Portella</span></div><div style="text-align:left"><font face="verdana, sans-serif">Rua Artur de Vasconcelos 151-Sul</font></div><div><div style="text-align:left">
<span style="font-family:verdana,sans-serif">64001-450 Teresina-PI</span></div><font face="verdana, sans-serif"><div style="text-align:left">Brazil</div><div style="text-align:left">Telephones: +55 86 3222-4377 (W), <div style="text-align:center;display:inline!important">
<div style="text-align:center;display:inline!important">+55 86 3221-3062 (W), </div></div><div style="text-align:center;display:inline!important">+55 86 3237-1075 (R). </div></div><div style="text-align:left"><br></div><div style="text-align:left">
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