Dear Carlos,<br /> I really think L. infantum do invade hindgut, I really do
not know how often it happens or it happens in any special situation. I think this
is not a 100% way to confirm subgenus Leishmania.<br /> We have no
problem in descriminate both subgenera by molecular approach.Here some
publications:<br />
<p style="LINE-HEIGHT: normal; MARGIN: 0cm 0cm 0pt; TEXT-AUTOSPACE: ;
mso-layout-grid-align: none" class="MsoNormal"><span style="FONT-FAMILY: GulliverRM;
FONT-SIZE: 12pt; mso-bidi-font-family: GulliverRM; mso-ansi-language: PT-BR"
lang="PT-BR">Pita Pereira, D., Alves, G.R., Souza, M.B., Brazil, R.P., Bertho, A.L.,
Figueiredo Barbosa, A., 2005. </span><span style="FONT-FAMILY: GulliverRM;
FONT-SIZE: 12pt; mso-bidi-font-family: GulliverRM">Identification of naturally
infected </span><span style="FONT-FAMILY: GulliverIT; FONT-SIZE: 12pt;
mso-bidi-font-family: GulliverIT">Lutzomyia intermdia </span><span
style="FONT-FAMILY: GulliverRM; FONT-SIZE: 12pt; mso-bidi-font-family:
GulliverRM">and </span><span style="FONT-FAMILY: GulliverIT; FONT-SIZE: 12pt;
mso-bidi-font-family: GulliverIT">Lutzomyia migonei </span><span style="FONT-FAMILY:
GulliverRM; FONT-SIZE: 12pt; mso-bidi-font-family: GulliverRM">with </span><span
style="FONT-FAMILY: GulliverIT; FONT-SIZE: 12pt; mso-bidi-font-family:
GulliverIT">Leishmania </span><span style="FONT-FAMILY: GulliverRM; FONT-SIZE: 12pt;
mso-bidi-font-family: GulliverRM">(</span><span style="FONT-FAMILY: GulliverIT;
FONT-SIZE: 12pt; mso-bidi-font-family: GulliverIT">Viannia</span><span
style="FONT-FAMILY: GulliverRM; FONT-SIZE: 12pt; mso-bidi-font-family: GulliverRM">)
</span><span style="FONT-FAMILY: GulliverIT; FONT-SIZE: 12pt; mso-bidi-font-family:
GulliverIT">braziliensis </span><span style="FONT-FAMILY: GulliverRM; FONT-SIZE:
12pt; mso-bidi-font-family: GulliverRM">in Rio de Janeiro (Brazil) revealed by a PCR
multiplex non-isotopic hybridisation assay. <strong style="mso-bidi-font-weight:
normal">Transactions of the Royal Society of Tropical Medicine and Hygiene 99,
905–913.
<p> </p>
</strong></span></p>
<p style="MARGIN: 0cm 0cm 10pt" class="MsoNormal"><span style="LINE-HEIGHT: 115%;
FONT-FAMILY: GulliverRM; FONT-SIZE: 12pt; mso-bidi-font-family: GulliverRM">
<p> </p>
</span></p>
<p style="LINE-HEIGHT: normal; MARGIN: 0cm 0cm 0pt; TEXT-AUTOSPACE: ;
mso-layout-grid-align: none" class="MsoNormal"><span style="FONT-FAMILY: GulliverRM;
FONT-SIZE: 12pt; mso-bidi-font-family: GulliverRM; mso-ansi-language: PT-BR"
lang="PT-BR">Saraiva, L., Andrade Filho, J.D., Silva, S.O., Andrade, A.S.R., Melo,
M.N., 2010. </span><span style="FONT-FAMILY: GulliverRM; FONT-SIZE: 12pt;
mso-bidi-font-family: GulliverRM">The molecular detection of different </span><span
style="FONT-FAMILY: GulliverIT; FONT-SIZE: 12pt; mso-bidi-font-family:
GulliverIT">Leishmania </span><span style="FONT-FAMILY: GulliverRM; FONT-SIZE: 12pt;
mso-bidi-font-family: GulliverRM">species within sand flies from a
<p> </p>
</span></p>
<p style="LINE-HEIGHT: normal; MARGIN: 0cm 0cm 0pt; TEXT-AUTOSPACE: ;
mso-layout-grid-align: none" class="MsoNormal"><span style="FONT-FAMILY: GulliverRM;
FONT-SIZE: 12pt; mso-bidi-font-family: GulliverRM">cutaneous and visceral
leishmaniasis sympatric area in Southeastern Brazil.
<p> </p>
</span></p>
<p style="MARGIN: 0cm 0cm 10pt" class="MsoNormal"><strong
style="mso-bidi-font-weight: normal"><span style="LINE-HEIGHT: 115%; FONT-FAMILY:
GulliverRM; FONT-SIZE: 12pt; mso-bidi-font-family: GulliverRM; mso-ansi-language:
PT-BR" lang="PT-BR">Memorias do Instituto Oswaldo Cruz 105,
1033–1039</span></strong><span style="LINE-HEIGHT: 115%; FONT-FAMILY:
GulliverRM; FONT-SIZE: 12pt; mso-bidi-font-family: GulliverRM; mso-ansi-language:
PT-BR" lang="PT-BR">.</span><span style="LINE-HEIGHT: 115%; FONT-SIZE: 12pt;
mso-ansi-language: PT-BR" lang="PT-BR">
<p> </p>
</span></p>
All the best,<br /> Reginaldo P. Brazil<br /><br /> <br
/>-- <br />Pesquisador Titular <br />Instituto Oswaldo Cruz <br />Lab.
Bioquímica & Fisiologia de Inseto <br />Av.Brasil 4365 <br />Pav.Leonidas
Deane-S/213 <br />Rio de Janeiro -21045-900 <br />Tel: 021-3865 8134 <br />Fax:
021-3865-8200 <br /><br /><br />> <br />Dear all, <br />> <br />> Dr. Ivete
Mendonça, in Teresina, Brazil, has consistently and <br />> systematically
been observing promastigotes xenodiagnosis performed in dogs <br />> in the
hindgut and rectal ampulla of Lutzomyia longipalpis. We are not sure <br />> if
they are really L. infantum or Viannia. Does anybody has observed the <br />>
same thing? <br />> <br />> By the way, any suggestions of primers to
discriminate Leishmania from <br />> Viannia? <br />> <br />> Many thanks,
<br />> <br />> Carlos. <br />>
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