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<DIV dir=ltr><FONT face=Arial color=#000000 size=2>Marvelous ! It is good to have more participation from as many groups as possible.</FONT></DIV>
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<DIV dir=ltr><FONT face=Arial size=2>KP</FONT></DIV></DIV>
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<FONT face=Tahoma size=2><B>From:</B> Dr. Dinesh Mondal [mailto:din63d@icddrb.org]<BR><B>Sent:</B> Sun 6/12/2011 3:39 AM<BR><B>To:</B> 'J.-C. Dujardin'; 'Raj'; nandlals@hotmail.com; Chang, Kwang-Poo; leish-l@lineu.icb.usp.br<BR><B>Cc:</B> 'Matthew Berriman'<BR><B>Subject:</B> RE: [Leish-l] cutaneous form of leish in Indian region<BR></FONT><BR></DIV>
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<P class=MsoNormal><SPAN style="FONT-SIZE: 11pt; COLOR: #1f497d; FONT-FAMILY: 'Calibri','sans-serif'">Dear JC, currently we are looking for the genetic diversity of L. donovani in Bangladesh. We will be happy to be a part of eventual muti-centre study. Thank you.</SPAN></P>
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<P class=MsoNormal><SPAN style="FONT-SIZE: 11pt; COLOR: #1f497d; FONT-FAMILY: 'Calibri','sans-serif'">Dinesh</SPAN></P>
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<P class=MsoNormal><B><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: 'Tahoma','sans-serif'">From:</SPAN></B><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: 'Tahoma','sans-serif'"> leish-l-bounces@lineu.icb.usp.br [mailto:leish-l-bounces@lineu.icb.usp.br] <B>On Behalf Of </B>J.-C. Dujardin<BR><B>Sent:</B> Wednesday, June 08, 2011 12:30 PM<BR><B>To:</B> Raj; nandlals@hotmail.com; Chang, Kwang-Poo; leish-l@lineu.icb.usp.br<BR><B>Cc:</B> Matthew Berriman<BR><B>Subject:</B> Re: [Leish-l] cutaneous form of leish in Indian region</SPAN></P></DIV></DIV>
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<P class=MsoNormal><SPAN style="FONT-SIZE: 11pt; COLOR: #1f497d; FONT-FAMILY: 'Calibri','sans-serif'">Dear all, I react only now after seeing a lot of mails passing. We are currently undertaking a major study on genetic diversity of L.donovani in the Indian sub-continent (including full genome sequencing). While most of the strains analyzed so far support the post-DDT bottleneck hypothesis (clonal expansion with low genetic homogeneity), we start finding rare genotypes which are very different from the major population. If you are interested, we might include your strains in our analysis.</SPAN></P>
<P class=MsoNormal><SPAN style="FONT-SIZE: 11pt; COLOR: #1f497d; FONT-FAMILY: 'Calibri','sans-serif'">Best regards</SPAN></P>
<P class=MsoNormal><SPAN style="FONT-SIZE: 11pt; COLOR: #1f497d; FONT-FAMILY: 'Calibri','sans-serif'">JC</SPAN></P>
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<P class=MsoNormal><SPAN style="FONT-SIZE: 11pt; COLOR: #1f497d; FONT-FAMILY: 'Calibri','sans-serif'">Prof. Dr Jean-Claude Dujardin</SPAN></P>
<P class=MsoNormal><SPAN style="FONT-SIZE: 11pt; COLOR: #1f497d; FONT-FAMILY: 'Calibri','sans-serif'">Head of Molecular Parasitology</SPAN></P>
<P class=MsoNormal><SPAN lang=NL-BE style="FONT-SIZE: 11pt; COLOR: #1f497d; FONT-FAMILY: 'Calibri','sans-serif'">Instituut voor Tropische Geneeskunde</SPAN></P>
<P class=MsoNormal><SPAN lang=NL-BE style="FONT-SIZE: 11pt; COLOR: #1f497d; FONT-FAMILY: 'Calibri','sans-serif'">Nationalestraat, 155</SPAN></P>
<P class=MsoNormal><SPAN lang=NL-BE style="FONT-SIZE: 11pt; COLOR: #1f497d; FONT-FAMILY: 'Calibri','sans-serif'">B-2000 Antwerpen</SPAN></P>
<P class=MsoNormal><SPAN lang=NL-BE style="FONT-SIZE: 11pt; COLOR: #1f497d; FONT-FAMILY: 'Calibri','sans-serif'">Belgium</SPAN></P>
<P class=MsoNormal><SPAN lang=NL-BE style="FONT-SIZE: 11pt; COLOR: #1f497d; FONT-FAMILY: 'Calibri','sans-serif'">Tel. 32.3.2476358 or 355</SPAN></P>
<P class=MsoNormal><SPAN lang=NL-BE style="FONT-SIZE: 11pt; COLOR: #1f497d; FONT-FAMILY: 'Calibri','sans-serif'">Fax. 32.3.2476359</SPAN></P></DIV>
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<P class=MsoNormal><B><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: 'Tahoma','sans-serif'">From:</SPAN></B><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: 'Tahoma','sans-serif'"> leish-l-bounces@lineu.icb.usp.br [mailto:leish-l-bounces@lineu.icb.usp.br] <B>On Behalf Of </B>Raj<BR><B>Sent:</B> donderdag 2 juni 2011 8:16<BR><B>To:</B> nandlals@hotmail.com; Chang, Kwang-Poo; leish-l@lineu.icb.usp.br<BR><B>Subject:</B> Re: [Leish-l] cutaneous form of leish in Indian region</SPAN></P></DIV></DIV>
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<P class=MsoNormal><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: 'Arial','sans-serif'">Hi KP,</SPAN></P></DIV>
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<P class=MsoNormal><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: 'Arial','sans-serif'">Certainly I will do. Please suggest Dr NL Sharma to do so.</SPAN></P></DIV>
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<P class=MsoNormal><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: 'Arial','sans-serif'">Raj</SPAN></P></DIV>
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<P class=MsoNormal><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: 'Arial','sans-serif'">----- Original Message ----- </SPAN></P></DIV>
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<P class=MsoNormal style="BACKGROUND: #e4e4e4"><B><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: 'Arial','sans-serif'">From:</SPAN></B><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: 'Arial','sans-serif'"> <A title=KwangPoo.Chang@rosalindfranklin.edu href="mailto:KwangPoo.Chang@rosalindfranklin.edu">Chang, Kwang-Poo</A> </SPAN></P></DIV>
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<P class=MsoNormal><B><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: 'Arial','sans-serif'">To:</SPAN></B><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: 'Arial','sans-serif'"> <A title=raj@cellabs.com.au href="mailto:raj@cellabs.com.au">Raj</A> ; <A title=hanafi.hanafi.ctr.eg@med.navy.mil href="mailto:hanafi.hanafi.ctr.eg@med.navy.mil">Hanafi, Hanafi CTR EG NAMRU3 </A>; <A title=nandlals@hotmail.com href="mailto:nandlals@hotmail.com">nandlals@hotmail.com</A> ; <A title=leish-l@lineu.icb.usp.br href="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</A> </SPAN></P></DIV>
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<P class=MsoNormal><B><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: 'Arial','sans-serif'">Sent:</SPAN></B><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: 'Arial','sans-serif'"> Thursday, June 02, 2011 8:41 AM</SPAN></P></DIV>
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<P class=MsoNormal><B><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: 'Arial','sans-serif'">Subject:</SPAN></B><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: 'Arial','sans-serif'"> RE: cutaneous form of leish in Indian region</SPAN></P></DIV>
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<P class=MsoNormal> </P></DIV>
<P class=MsoNormal><SPAN style="FONT-SIZE: 10pt; COLOR: navy; FONT-FAMILY: 'Arial','sans-serif'">Great if Dr. Sharma would be able to send serum samples to you for evaluating your species-specific exo-antigens.</SPAN></P>
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<P class=MsoNormal><SPAN style="FONT-SIZE: 10pt; COLOR: navy; FONT-FAMILY: 'Arial','sans-serif'">KP</SPAN></P>
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<P class=MsoNormal><B><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: 'Tahoma','sans-serif'">From:</SPAN></B><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: 'Tahoma','sans-serif'"> Raj [mailto:raj@cellabs.com.au] <BR><B>Sent:</B> Tuesday, May 31, 2011 9:18 PM<BR><B>To:</B> Hanafi, Hanafi CTR EG NAMRU3 ; Chang, Kwang-Poo; <A href="mailto:nandlals@hotmail.com">nandlals@hotmail.com</A>; <A href="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</A><BR><B>Subject:</B> cutaneous form of leish in Indian region</SPAN></P></DIV>
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<P class=MsoNormal><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: 'Arial','sans-serif'">G'Day Dr Hanafi,</SPAN></P></DIV>
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<P class=MsoNormal><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: 'Arial','sans-serif'">My enquiry was to know whether <EM><SPAN style="FONT-FAMILY: 'Arial','sans-serif'">L. donovani</SPAN></EM> in India produces a cutaneous form of lesion or not (I am not referring to PKDL form). There are many reports from SriLanka about the cutaneous manifestations by <EM><SPAN style="FONT-FAMILY: 'Arial','sans-serif'">L. donovani</SPAN></EM>. The PKDL although is a cutaneous response appears very late in the infection process and this is ascribed to be a protective response against the parasite. </SPAN></P></DIV>
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<P class=MsoNormal><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: 'Arial','sans-serif'"></SPAN> </P></DIV>
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<P class=MsoNormal><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: 'Arial','sans-serif'">Prof Chang mentioned </SPAN></P></DIV>
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<P class=MsoNormal><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: 'Arial','sans-serif'">"Analyses of several batches of basically clinical CL samples from Dr. Sharma showed evidence of L infantum, but also L tropica as well as a mixture of the two in one sample. This is the same picture we have noted for samples from Hatay, Turkey." This is from Sutlej region." It means this cutaneous complex requires some investigation. </SPAN></P></DIV>
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<P class=MsoNormal><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: 'Arial','sans-serif'"></SPAN> </P></DIV>
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<P class=MsoNormal><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: 'Arial','sans-serif'">I raise species-specific exo-antigens from <EM><SPAN style="FONT-FAMILY: 'Arial','sans-serif'">L. tropica</SPAN></EM> and <EM><SPAN style="FONT-FAMILY: 'Arial','sans-serif'">L infantum</SPAN></EM> and I am prepared to test the serum /plasma samples for presence of antibodies. If Dr NL Sharma is prepared to send me specimens as well as clinical plasma or serum samples I would be interested to investigate the antibody response in these situations. </SPAN></P></DIV>
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<P class=MsoNormal><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: 'Arial','sans-serif'"></SPAN> </P></DIV>
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<P class=MsoNormal><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: 'Arial','sans-serif'">Dr Raj</SPAN></P></DIV>
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<P class=MsoNormal><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: 'Arial','sans-serif'">Cellabs</SPAN></P></DIV>
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<P class=MsoNormal><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: 'Arial','sans-serif'">----- Original Message ----- </SPAN></P>
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<P class=MsoNormal><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: 'Arial','sans-serif'">From: "Hanafi, Hanafi CTR EG NAMRU3 " <<A href="mailto:hanafi.hanafi.ctr.eg@med.navy.mil">hanafi.hanafi.ctr.eg@med.navy.mil</A>></SPAN></P></DIV>
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<P class=MsoNormal><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: 'Arial','sans-serif'">To: "Raj" <<A href="mailto:raj@cellabs.com.au">raj@cellabs.com.au</A>>; <<A href="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</A>></SPAN></P></DIV>
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<P class=MsoNormal><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: 'Arial','sans-serif'">Sent: Tuesday, May 31, 2011 6:19 PM</SPAN></P></DIV>
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<P class=MsoNormal><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: 'Arial','sans-serif'">Subject: RE: [Leish-l] Leish-l Digest, Vol 41, Issue 9</SPAN></P></DIV></DIV>
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<P class=MsoNormal><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: 'Arial','sans-serif'"></SPAN> </P></DIV>
<P class=MsoNormal><SPAN style="FONT-SIZE: 10pt; FONT-FAMILY: 'Arial','sans-serif'">> Dear Dr Raj:<BR>> <BR>> I think you mean PKDL (post kala azar dermal leishmanisis) caused by L.<BR>> donovani and it usually seen as a late complication of the disease. PKDL has<BR>> been reported in India, Kenya and Sudan.<BR>> <BR>> Thank you <BR>> R/<BR>> Hanafi<BR>> <BR>> Hanafi A. Hanafi, Ph. D.<BR>> Medical Research Scientist<BR>> Vector Biology Research Program<BR>> U.S. Naval Medical Research Unit No.3<BR>> Cairo, Egypt<BR>> Tel & Fax: ++2-02-2342-3090<BR>> E-mail: <A href="mailto:hanafi.hanafi.ctr.eg@med.navy.mil">hanafi.hanafi.ctr.eg@med.navy.mil</A><BR>> <A href="mailto:yahanafi@yahoo.com">yahanafi@yahoo.com</A><BR>> </SPAN></P></DIV>
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