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<DIV><FONT face=Arial size=2>Dear Bob, </FONT></DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>hybridization takes place in the vector but I am
not sure if our finding in Cukurova brings a supporting evidence for
this. I can imagine also other scenarios, for example there are seasonal
workers in this area, man comming back from army servis etc. </FONT></DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>Experimentally we confirmed that at least two local
vectors, P. perniciosus and P. tobbi, are highly susceptible to both,
"typical" L. donovani (LV9) as well MON1 L. infantum. They are, of course,
highly susceptible to the CUK strains and transmit them into mice (Carla Maia
presented this in ISOPS this April). For me this is not surprising as P.
perniciosus and P. tobbi are Larroussius species and permissive
vectors.</FONT></DIV>
<DIV><FONT face=Arial size=2></FONT> </DIV>
<DIV><FONT face=Arial size=2>Best wishes</FONT></DIV>
<DIV><FONT face=Arial size=2>Petr</FONT></DIV>
<DIV><FONT face=Arial size=2>PS: Just to answer briefly older question about
hybrids (I sent this reply before only to Patrick as I feel that discussion is
becomming spread too much): based on techniques described in the manuscript we
originally thought that these strains are L. infantum (there is nothing about
hybrids in the paper). Recently we obtained new data including the whole genome
sequence and this allow me to mention that the CUK strain is probably a
hybrid.<BR><BR></FONT></DIV>
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<DIV style="FONT: 10pt arial">----- Original Message ----- </DIV>
<DIV
style="BACKGROUND: #e4e4e4; FONT: 10pt arial; font-color: black"><B>From:</B>
<A title=killickendrick@wanadoo.fr
href="mailto:killickendrick@wanadoo.fr">BobKillick-Kendrick</A> </DIV>
<DIV style="FONT: 10pt arial"><B>To:</B> <A
title=patrick.bastien@univ-montp1.fr
href="mailto:patrick.bastien@univ-montp1.fr">Patrick Bastien</A> ; <A
title=volf@cesnet.cz href="mailto:volf@cesnet.cz">Petr Volf</A> </DIV>
<DIV style="FONT: 10pt arial"><B>Cc:</B> <A
title=f-pratlong@chu-montpellier.fr
href="mailto:f-pratlong@chu-montpellier.fr">Pratlong F</A> ; <A
title=laurence.lachaud@chu-nimes.fr
href="mailto:laurence.lachaud@chu-nimes.fr">Lachaud</A> ; <A
title=christophe.ravel@univ-montp1.fr
href="mailto:christophe.ravel@univ-montp1.fr">Ravel</A> ; <A
title=leish-l@lineu.icb.usp.br
href="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</A> </DIV>
<DIV style="FONT: 10pt arial"><B>Sent:</B> Wednesday, June 08, 2011 5:59
PM</DIV>
<DIV style="FONT: 10pt arial"><B>Subject:</B> Re: [Leish-l] inquiry</DIV>
<DIV><BR></DIV>
<DIV>If there are hybrids between <EM>infantum</EM> and <EM>donovani</EM> in
Turkey, the cross must have taken place in a vector. Petr, is there a sand fly
species there that transmits both parasites? If not, how can there be a
hybrid?</DIV>
<DIV> Bob</DIV>
<BLOCKQUOTE
style="PADDING-RIGHT: 0px; PADDING-LEFT: 5px; MARGIN-LEFT: 5px; BORDER-LEFT: #000000 2px solid; MARGIN-RIGHT: 0px">
<DIV style="FONT: 10pt arial">----- Original Message ----- </DIV>
<DIV
style="BACKGROUND: #e4e4e4; FONT: 10pt arial; font-color: black"><B>From:</B>
<A title=patrick.bastien@univ-montp1.fr
href="mailto:patrick.bastien@univ-montp1.fr">Patrick Bastien</A> </DIV>
<DIV style="FONT: 10pt arial"><B>To:</B> <A title=volf@cesnet.cz
href="mailto:volf@cesnet.cz">Petr Volf</A> </DIV>
<DIV style="FONT: 10pt arial"><B>Cc:</B> <A
title=f-pratlong@chu-montpellier.fr
href="mailto:f-pratlong@chu-montpellier.fr">Pratlong F</A> ; <A
title=laurence.lachaud@chu-nimes.fr
href="mailto:laurence.lachaud@chu-nimes.fr">Lachaud</A> ; <A
title=christophe.ravel@univ-montp1.fr
href="mailto:christophe.ravel@univ-montp1.fr">Ravel</A> ; <A
title=leish-l@lineu.icb.usp.br
href="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</A> </DIV>
<DIV style="FONT: 10pt arial"><B>Sent:</B> Friday, June 03, 2011 3:37
PM</DIV>
<DIV style="FONT: 10pt arial"><B>Subject:</B> Re: [Leish-l] inquiry</DIV>
<DIV><BR></DIV>Dear Petr,<BR><BR>Two comments :<BR>1) I am curious to know
how you define a hybrid between L. infantum <BR>and L. donovani,
knowing that these species are quite difficult to <BR>differentiate by
simple sequence analysis. Is it using the MLST method <BR>described in
your paper attached ? Are the genes you are using single <BR>copy or
duplicated ? Have you used isoenzymes for confirmation ?<BR><BR>2) Talking
of such, I am always puzzled when I see efforts for <BR>correlating
molecular and isoenzyme-based phenogramms (Fig. 4, your <BR>article
attached). Worse, your text clearly states that the strains
<BR>analyzed are "unambiguously distinct from the MON-1 zymodeme (...)
of <BR>L. infantum". This is particularly risky, as it is to use
zymodeme <BR>names (MON-...) in a phylogenetic reconstruction using
other intrinsic <BR>(here molecular) criteria. Indeed, a zymodeme may
include different <BR>genotypes and a genotype may theoretically cover
several zymodemes. A <BR>zymodeme is nothing else than an "operational
taxonomic unit" used for <BR>classification (phenetics) (see the
famous paper by Rioux et al. <BR>attached); as such, it has no
biological nor evolutionary signification.<BR>Similarly, when you write
"Among accessible isolates typed using the <BR>MLST method, our strain
is close to MON-188, the L. infantum strain <BR>isolated by Gramiccia
(Pratlong et al., 2003)(...)", the MON-... is <BR>abusively
assimilated to a given strain (here ISS800), whereas one <BR>zymodeme
normally clusters several strains, that might be found <BR>clustered
differently using your method or another method.<BR>Another important point
is that the MON-... is defined by the <BR>examination of a series of
15 given iso-enzymes, but this is also <BR>dependent upon the
technique used (and, to a lesser degree, to the <BR>person/research
group who defines it). That is why Rioux et al. <BR>recommended that
each identifying center use its own code (such as <BR>MON- for
Montpellier or LON- for London). Indeed, MON-188 in <BR>Montpellier
migh tbe different from MON-188 in another center if the <BR>system
used is not exactly the same.<BR>All this may seem trivial, but there is a
real danger, as it slowly <BR>introduces in the minds of our
colleagues scientists that different <BR>classifications are
equivalent.<BR>I know that I am touching on a question which can yield hours
of <BR>debate and thousands of e-mails; so, although I am sure you
will want <BR>to reply to this, please note that I do not want to
lauch such a <BR>debate online !<BR><BR>Best
wishes<BR>Patrick<BR><BR>Professeur Patrick Bastien<BR>Laboratoire de
Parasitologie-Mycologie, Faculté de Médecine<BR>UMR MIVEGEC (CNRS 5290 - IRD
224 - Université Montpellier 1)<BR>Centre National de Référence des
Leishmania<BR>CHU de Montpellier<BR>39 Avenue Charles Flahault<BR>34295
Montpellier Cedex 5, France<BR><BR>Petr Volf <<A
href="mailto:volf@cesnet.cz">volf@cesnet.cz</A>> a écrit :<BR><BR>>
Re: [Leish-l] inquiryHi K.P.,<BR>> in Cukurova region, Turkey, cutaneous
L. infantum (now it seems that <BR>> we are dealing with
L.donovani/L. infantum hybrid) grew very <BR>> poorely if we
isolated them from patients. Only 1 of 25 isolations <BR>>
was succesfull. However, the same strain (confirmed by
molecular <BR>> methods) grew repeatedly and very well if we
isolated them from <BR>> sandflies. It might be useful for
Dr. Sharma try to get isolates <BR>> from sand flies. It is
very laborious but very useful, in Cukurova <BR>> we got
about dozen of isolates by this method (all identical).<BR>> Patients are
rK39 negative, see attached paper.<BR>> Best wishes<BR>>
Petr<BR>> ----- Original Message -----<BR>>
From: Chang, Kwang-Poo<BR>> To: Sharman ; Hiro Goto ; elfadil
abass<BR>> Cc: <A
href="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</A><BR>>
Sent: Sunday, May 22, 2011 1:39 AM<BR>> Subject: Re:
[Leish-l] inquiry<BR>><BR>><BR>> Dr. NL Sharma has been
working on an important CL endemic area <BR>> along the
Satluj River valley to the south of Himalaya in India <BR>>
(Please correct me should I be wrong for anything I said here
and <BR>> below). I had the good fortune of visiting the site
several years <BR>> back courtesy of Dr.
Sharma.<BR>><BR>> I believe Dr. Sharma's finding is
important, since the parasites <BR>> there are different from
the familiar Indian L donovani in Bihar. <BR>> The parasites
are refratory to in vitro cultivation. They do <BR>>
differentiate into promastigotes and may grow a little, but
can't <BR>> really be subcultured to establish passageable
lines. This is very <BR>> much reminescent of L infantum in
the Mediterranean area. I recall <BR>> Dr. Sharma has also
found rK39+ dogs, if I remember correctly. If <BR>> so,
Satluj river valley is endemic to the infantile
CL.<BR>><BR>> Analyses of several batches of basically
clinical CL samples from <BR>> Dr. Sharma showed evidence of
L infantum, but also L tropica as well <BR>> as a mixture of
the two in one sample. This is the same picture we <BR>> have
noted for samples from Hatay, Turkey.<BR>><BR>> These
observations make me wonder a lot about our current <BR>>
knowledge on the clinico-epidemiology based on data collected
<BR>> previously by analyses of cultured promastigotes from one or
few <BR>> 'representative samples'. Nowaday, technology makes
it very doable <BR>> to work with biological samples for
Leish DNAs directly from sand <BR>> flies, patients and
reservoir animals.<BR>><BR>>
KP<BR>><BR>><BR>><BR>>
------------------------------------------------------------------------------<BR>>
From: <A
href="mailto:leish-l-bounces@lineu.icb.usp.br">leish-l-bounces@lineu.icb.usp.br</A>
on behalf of Sharman<BR>> Sent: Fri 5/20/2011 11:31
PM<BR>> To: Hiro Goto; elfadil abass<BR>> Cc:
<A
href="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</A><BR>>
Subject: Re: [Leish-l] inquiry<BR>><BR>><BR>> Dear
all<BR>> I agree with Hiro gito, We are working on a focus
where the CL is<BR>> predominantly caused by L. donovani, and
the rK 39 STRIPS GIVE POSITIVE<BR>> RESULTS WITH SERA as well
as serous exudate from the lesion. The <BR>> results
are<BR>> dependent upon species.<BR>> NL
Sharma<BR>><BR>>
--------------------------------------------------<BR>> From:
"Hiro Goto" <<A
href="mailto:hgoto@usp.br">hgoto@usp.br</A>><BR>> Sent:
Wednesday, May 18, 2011 12:09 AM<BR>> To: "elfadil abass"
<<A
href="mailto:elfadil_abass@yahoo.com">elfadil_abass@yahoo.com</A>><BR>>
Cc: <<A
href="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</A>><BR>>
Subject: Re: [Leish-l] inquiry<BR>><BR>> > Dear
all,<BR>> > In our oppinion, DAT and rK39 for those
samples are not indicated since<BR>> > these tests
are produced for the diagnosis of visceral
leishmaniasis.<BR>> > In case of tegumentary
leishmaniasis, it is very appropriate the<BR>> >
observation of J.J. Shaw appointing species specificity of
antibody<BR>> > response in these cases. Titers of
antibodies are in general low <BR>> in
these<BR>> > cases therefore depending on the species, it
may result <BR>> negative. We have<BR>>
> published a review recently in Expert Rev. Anti Infect. Ther.
8(4),<BR>> > 419?433 (2010), Current diagnosis and
treatment of cutaneous and<BR>> > mucocutaneous
leishmaniasis, where we raise this point.<BR>> > Hiro
Goto<BR>> ><BR>> > Citando elfadil abass
<<A
href="mailto:elfadil_abass@yahoo.com">elfadil_abass@yahoo.com</A>>:<BR>>
><BR>> >> Dear all I would recommend using DAT and
rK39 strip test to measure<BR>> >>
antibody<BR>> >> responses and to evaluate the
diagnostic efficiency for both tests in<BR>> >>
such group<BR>> >> of patients.<BR>>
>><BR>> >> Elfadil
Abass<BR>> >> <BR>>
________________________________________________________________________________<BR>>
>> Institute of Medical Microbiology and Hospital
Hygiene<BR>> >> Philipps University
Marburg<BR>> >> BMFZ / Hans-Meerwein Straße
2<BR>> >> D-35033 Marburg, Germany<BR>>
>> <BR>>
________________________________________________________________________________<BR>>
>><BR>> >><BR>>
>><BR>> >><BR>> >>
________________________________<BR>> >> From: Nuha
Nuwayri-Salti <<A
href="mailto:racha@aub.edu.lb">racha@aub.edu.lb</A>><BR>>
>> To: saad saad <<A
href="mailto:saad1426@gmail.com">saad1426@gmail.com</A>>; "<A
href="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</A>"<BR>>
>> <<A
href="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</A>><BR>>
>> Sent: Fri, May 13, 2011 9:39:33 AM<BR>> >>
Subject: Re: [Leish-l] inquiry<BR>>
>><BR>> >> Dear Saad first precaution to take is
to separate your samples into<BR>> >>
several<BR>> >> portions each (at least 5 each being no
more than a few hundred(200-400)<BR>> >> microliters).
This is a necessary precaution to avoid freezing and<BR>>
>> thawing<BR>> >> several times the same sample
which will be the case should you do<BR>> >>
different<BR>> >> studies at different times which is
unavoidable.<BR>> >><BR>> >> What
you can do is correlate the type(ulcerated, abscess,
furuncle<BR>> >> etc.. ) the<BR>>
>> locale, the number and age of lesions with the levels
of <BR>> antibody in the<BR>> >>
sera of<BR>> >> these patgients and also monitor cell
mediated immunity with leishmanin<BR>> >>
skin<BR>> >> test!<BR>> >> I have
just published (in print) an article about having
circulating<BR>> >> parasites<BR>>
>> in some of these patients with apparently pure cutaneous disease.
It is<BR>> >> the<BR>> >> first
paper that revealed this fact. It would be interesting to
confirm<BR>> >> or<BR>> >>
de-confirm this fact repeating what we did.<BR>> >>
Best wishes<BR>> >><BR>> >> Nuha
Nuwayri-Salti MD<BR>> >><BR>>
>><BR>> >><BR>> >>
AOA<BR>> >> Medical Honor Society<BR>>
>><BR>> >><BR>>
>><BR>> >><BR>> >>
-----Original Message-----<BR>> >> From: <A
href="mailto:leish-l-bounces@lineu.icb.usp.br">leish-l-bounces@lineu.icb.usp.br</A><BR>>
>> [mailto:leish-l-bounces@lineu.icb.usp.br]<BR>>
>> On Behalf Of saad saad<BR>> >> Sent: Friday,
April 29, 2011 11:23 PM<BR>> >> To: <A
href="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</A><BR>>
>> Subject: [Leish-l] inquiry<BR>>
>><BR>> >> Dear all<BR>> >>
Hi. i am Saad from Saudi Arabia. i have 60 sera from positive case
of<BR>> >> CL from south west of the country. I would
like to have your<BR>> >> recommendations to start a
good research line in CL using these sera.<BR>> >>
Thanks in advance for your help<BR>> >>
_______________________________________________<BR>> >>
Leish-l mailing list<BR>> >> <A
href="mailto:Leish-l@lineu.icb.usp.br">Leish-l@lineu.icb.usp.br</A><BR>>
>> <A
href="http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l">http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l</A><BR>>
>> _______________________________________________<BR>>
>> Leish-l mailing list<BR>> >> <A
href="mailto:Leish-l@lineu.icb.usp.br">Leish-l@lineu.icb.usp.br</A><BR>>
>> <A
href="http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l">http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l</A><BR>>
>><BR>> ><BR>>
><BR>> ><BR>> > Profa. Dra. Hiro
Goto<BR>> > Laboratório de Soroepidemiologia e
Imunobiologia<BR>> > Instituto de Medicina Tropical de São
Paulo, USP<BR>> > Av. Dr. Enéas de Carvalho Aguiar, 470,
prédio II, quarto andar<BR>> > 05403-000 - São Paulo,
SP<BR>> > Tel. +55-11-3061 7023, 3061 7056 ou 3061
7027<BR>> > Fax. +55-11-3061-8270<BR>>
><BR>> >
_______________________________________________<BR>> >
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