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<div class=Section1>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>It is important to keep up the
communication. The genetic diversity project is an enormous one that will
undoubtedly need many groups to work together.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>KP <o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'><o:p> </o:p></span></font></p>
<div>
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face="Times New Roman"><span style='font-size:12.0pt'>
<hr size=2 width="100%" align=center tabindex=-1>
</span></font></div>
<p class=MsoNormal><b><font size=2 face=Tahoma><span style='font-size:10.0pt;
font-family:Tahoma;font-weight:bold'>From:</span></font></b><font size=2
face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma'> neeloo singh
[mailto:neeloo888@yahoo.com] <br>
<b><span style='font-weight:bold'>Sent:</span></b> Wednesday, June 08, 2011
3:16 AM<br>
<b><span style='font-weight:bold'>To:</span></b> Madhumita Manna; leish-l@lineu.icb.usp.br;
Chang, Kwang-Poo<br>
<b><span style='font-weight:bold'>Subject:</span></b> Re: [Leish-l] inquiry</span></font><o:p></o:p></p>
</div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><o:p> </o:p></span></font></p>
<table class=MsoNormalTable border=0 cellspacing=0 cellpadding=0
style='border-collapse:collapse'>
<tr>
<td valign=top style='padding:0in 0in 0in 0in'>
<p class=MsoNormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>Dear Madhumita<br>
I have recently completed my DBT project carrying out whole genome sequencing
of two clinical isolates from <st1:place w:st="on">Bihar</st1:place>, the
assembly of the genomes brought forth some disturbing results, presently i am
writing the report to submit to DBT as well as send for publication<br>
<br>
in July I have booked sequencer for running another very recent solate, <br>
although this work involves a lot of finances, following the ongoing
discussion, <br>
if Dr Sharma would like then I am thinking of including one of Dr Sharma's
isolates too, so hopefully by August we can have some results<br>
since I have already conducted this whole exercise of whole genome
sequencing,<br>
(at this stage i would like to acknowledge the invaluable assistance of
Peter)<br>
irrespective of the results obtained from previous sequencing, the stage is
already set to smoothly repeat again albeit the enormous finances
requires,<br>
<br>
Madhumita, for the depository, Leishmaniasis Research Society (<st1:country-region
w:st="on"><st1:place w:st="on">India</st1:place></st1:country-region>) would
be very glad to help financially and scientifically to set
up, <br>
neeloo<br>
<br>
<br>
--- On <b><span style='font-weight:bold'>Tue, 6/7/11, Chang, Kwang-Poo <i><span
style='font-style:italic'><KwangPoo.Chang@rosalindfranklin.edu></span></i></span></b>
wrote:<o:p></o:p></span></font></p>
<p class=MsoNormal style='margin-bottom:12.0pt'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'><br>
From: Chang, Kwang-Poo <KwangPoo.Chang@rosalindfranklin.edu><br>
Subject: Re: [Leish-l] inquiry<br>
To: "Madhumita Manna" <madhumita.manna09@gmail.com>,
leish-l@lineu.icb.usp.br<br>
Date: Tuesday, June 7, 2011, 8:02 AM<o:p></o:p></span></font></p>
<div id=yiv573729320>
<div id=yiv573729320idOWAReplyText50965>
<div>
<p class=MsoNormal><font size=2 color=black face=Arial><span
style='font-size:10.0pt;font-family:Arial;color:black'>I'll be happy to make
my collection of Leishmania availalbe to anyone who is interested in setting
up a 'permanent' depository of such samples.</span></font><o:p></o:p></p>
</div>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>KP</span></font><o:p></o:p></p>
</div>
</div>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'><o:p> </o:p></span></font></p>
<div class=MsoNormal align=center style='text-align:center'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'>
<hr size=2 width="100%" align=center tabindex=0>
</span></font></div>
<p class=MsoNormal style='margin-bottom:12.0pt'><b><font size=2 face=Tahoma><span
style='font-size:10.0pt;font-family:Tahoma;font-weight:bold'>From:</span></font></b><font
size=2 face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma'>
Madhumita Manna [mailto:madhumita.manna09@gmail.com]<br>
<b><span style='font-weight:bold'>Sent:</span></b> Tue 6/7/2011 1:29 AM<br>
<b><span style='font-weight:bold'>To:</span></b> Chang, Kwang-Poo;
leish-l@lineu.icb.usp.br<br>
<b><span style='font-weight:bold'>Subject:</span></b> Re: [Leish-l] inquiry</span></font><o:p></o:p></p>
</div>
<div>
<div>
<p class=MsoNormal><span class=yiv573729320apple-style-span><font size=1
face=Arial><span style='font-size:6.5pt;font-family:Arial'>Dear KP, <o:p></o:p></span></font></span></p>
<div>
<p class=MsoNormal><font size=1 face=Arial><span style='font-size:6.5pt;
font-family:Arial'>Thank you very much for your valuable suggestions and
information. I should talk to Neeloo. Regarding the parasites, I can
give you the Iist. It is in my thesis. Unfortunately, IICB has lost most
of them. Many senior Scientists have retired who were in charge of the job.
</span></font><o:p></o:p></p>
</div>
<div>
<p class=MsoNormal><font size=1 face=Arial><span style='font-size:6.5pt;
font-family:Arial'><o:p> </o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=1 face=Arial><span style='font-size:6.5pt;
font-family:Arial'>Kind regards, <o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=1 face=Arial><span style='font-size:6.5pt;
font-family:Arial'> Madhumita<o:p></o:p></span></font></p>
</div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'><o:p> </o:p></span></font></p>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>On Mon, Jun 6, 2011 at 11:49 PM, Chang, Kwang-Poo <<a
href="/mc/compose?to=KwangPoo.Chang@rosalindfranklin.edu" target="_blank"
ymailto="mailto:KwangPoo.Chang@rosalindfranklin.edu">KwangPoo.Chang@rosalindfranklin.edu</a>>
wrote:<o:p></o:p></span></font></p>
<div>
<div>
<ol start=1 type=1>
<li class=MsoNormal style='color:navy;mso-margin-top-alt:auto;mso-margin-bottom-alt:
auto;mso-list:l8 level1 lfo1'><font size=2 color=navy face=Arial><span
style='font-size:10.0pt;font-family:Arial'>Neeloo singh has been doing
genomic sequencing of some Indian isolates. You may want to coordinate
with her.</span></font> <o:p></o:p></li>
<li class=MsoNormal style='color:navy;mso-margin-top-alt:auto;mso-margin-bottom-alt:
auto;mso-list:l8 level1 lfo1'><font size=2 color=navy face=Arial><span
style='font-size:10.0pt;font-family:Arial'>Do you have a list of Dr.
Bhaduri’s collection in IICB ? If so, is it available for checking
against what I have ?</span></font> <o:p></o:p></li>
<li class=MsoNormal style='color:navy;mso-margin-top-alt:auto;mso-margin-bottom-alt:
auto;mso-list:l8 level1 lfo1'><font size=2 color=navy face=Arial><span
style='font-size:10.0pt;font-family:Arial'>Deep sequencing of Leishmania
genomes is very doable and not terribly expensive. The bottle-neck is
the bioinformatic analyses. There is no user friendly program for Leish
genomics in so far as I know. Neeloo may have some useful information
there.</span></font> <o:p></o:p></li>
</ol>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
<p class=yiv573729320msonormal><font size=2 color=navy face=Arial><span
style='font-size:10.0pt;font-family:Arial;color:navy'>KP</span></font><o:p></o:p></p>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
<div>
<div class=MsoNormal align=center style='text-align:center'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'>
<hr size=2 width="100%" align=center>
</span></font></div>
<p class=yiv573729320msonormal><b><font size=2 face=Tahoma><span
style='font-size:10.0pt;font-family:Tahoma;font-weight:bold'>From:</span></font></b><font
size=2 face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma'>
Madhumita Manna [mailto:<a href="/mc/compose?to=madhumita.manna09@gmail.com"
target="_blank" ymailto="mailto:madhumita.manna09@gmail.com">madhumita.manna09@gmail.com</a>]
<br>
<b><span style='font-weight:bold'>Sent:</span></b> Monday, June 06, 2011 4:45
AM</span></font><o:p></o:p></p>
<div>
<div>
<p class=MsoNormal><font size=2 face=Tahoma><span style='font-size:10.0pt;
font-family:Tahoma'><br>
<b><span style='font-weight:bold'>To:</span></b> Chang, Kwang-Poo<br>
<b><span style='font-weight:bold'>Subject:</span></b> Re: [Leish-l] inquiry</span></font><o:p></o:p></p>
</div>
</div>
</div>
<div>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>Dear KP,<o:p></o:p></span></font></p>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>Thank you very much for your valuable suggestions. I
am trying to hit as many genetic sites are possible and we have a plan to
establish a depository of isolates of KA, PKDL for future works. Prof.
Bhaduri tried hard to set up this kind of bank and at that time we have
collected and deposited a good number of isolates in that Parasite Bank at
IICB. <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>For entire genome sequencing, a big group needs to
work together and for that collaboration with other group is solicited.<o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>Kind regards,<o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal style='margin-bottom:12.0pt'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'>Madhumita<o:p></o:p></span></font></p>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>On Sun, Jun 5, 2011 at 11:05 PM, Chang, Kwang-Poo <<a
href="/mc/compose?to=KwangPoo.Chang@rosalindfranklin.edu" target="_blank"
ymailto="mailto:KwangPoo.Chang@rosalindfranklin.edu">KwangPoo.Chang@rosalindfranklin.edu</a>>
wrote:<o:p></o:p></span></font></p>
<div>
<div>
<div>
<p class=yiv573729320msonormal><font size=2 color=black face=Arial><span
style='font-size:10.0pt;font-family:Arial;color:black'>Dear Madhumitsa,</span></font><o:p></o:p></p>
</div>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>You are doing something very
important. I re-iterate my suggestions as follows:</span></font><o:p></o:p></p>
</div>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>1. Examine Leishmania grown in
cultures as well as in the original samples</span></font><o:p></o:p></p>
</div>
<div>
<p class=yiv573729320msonormal><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>2. Evaluate as many genetic sites
as possible, preferably the entire genomes</span></font><o:p></o:p></p>
</div>
<div>
<p class=yiv573729320msonormal><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>3. Set up a depository center for
Leishmana samples</span></font><o:p></o:p></p>
</div>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>KP</span></font><o:p></o:p></p>
</div>
</div>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
<div class=MsoNormal align=center style='text-align:center'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'>
<hr size=2 width="100%" align=center>
</span></font></div>
<div>
<p class=yiv573729320msonormal><b><font size=2 face=Tahoma><span
style='font-size:10.0pt;font-family:Tahoma;font-weight:bold'>From:</span></font></b><font
size=2 face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma'>
Madhumita Manna [mailto:<a href="/mc/compose?to=madhumita.manna09@gmail.com"
target="_blank" ymailto="mailto:madhumita.manna09@gmail.com">madhumita.manna09@gmail.com</a>]</span></font><o:p></o:p></p>
</div>
<p class=yiv573729320msonormal><b><font size=2 face=Tahoma><span
style='font-size:10.0pt;font-family:Tahoma;font-weight:bold'>Sent:</span></font></b><font
size=2 face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma'> Sun
6/5/2011 7:14 AM<br>
<b><span style='font-weight:bold'>To:</span></b> Chang, Kwang-Poo</span></font><o:p></o:p></p>
<div>
<div>
<p class=yiv573729320msonormal><font size=2 face=Tahoma><span
style='font-size:10.0pt;font-family:Tahoma'><br>
<b><span style='font-weight:bold'>Subject:</span></b> Re: [Leish-l] inquiry</span></font><o:p></o:p></p>
</div>
</div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<div>
<div>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>Dear KP <o:p></o:p></span></font></p>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>I have collected the isolate in the year 2010 from a
patient admitted in <st1:place w:st="on"><st1:PlaceName w:st="on">Calcutta</st1:PlaceName>
<st1:PlaceName w:st="on">National</st1:PlaceName> <st1:PlaceName w:st="on">Medical</st1:PlaceName>
<st1:PlaceType w:st="on">College</st1:PlaceType></st1:place> and done the
RAPD, RFLP and sequencing in recent past. The publication is underway. The
work is in collaboration with Dr. Syamal Roy, IICB and we are interested in
Satluj river valley isolates as well as we are trying to isolate more
clinical isolates from other parts of India. In this particular case, the
transformed promastigotes were cultured at <st1:place w:st="on"><st1:PlaceName
w:st="on">Bethune</st1:PlaceName> <st1:PlaceType w:st="on">College</st1:PlaceType></st1:place>,
Kolkata where we did not maintain any L.tropica strain or UR6 that time. In
2010, we isolated five isolates collected from Kolkata of which four
patients from Bihar,one from <st1:place w:st="on">West Bengal</st1:place>.
So, there was no chance of mixing in this particular case which might
result occasionally due to human errors as you told and also Prof Schnur once
cautioned about the identity of some Indian isolates long back in 1981 when
he had screened 84 isolates and he apparently got one L.tropica, if I am not
wrong .<o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>I have dealt with UR6 during my Ph.D works in 1993
onwards and have seen it close to L.tropica [about 72% homology in Jaccard's
similarity index calculated by isozyme and RAPD works]. Recently, by ITS1
RFLP, UR6 is again found out to be matching with L.tropica. <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>kind regards,<o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>Madhumita<o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal style='margin-bottom:12.0pt'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>On Sat, Jun 4, 2011 at 11:59 PM, Chang, Kwang-Poo <<a
href="/mc/compose?to=KwangPoo.Chang@rosalindfranklin.edu" target="_blank"
ymailto="mailto:KwangPoo.Chang@rosalindfranklin.edu">KwangPoo.Chang@rosalindfranklin.edu</a>>
wrote:<o:p></o:p></span></font></p>
<div>
<div>
<p class=yiv573729320msonormal><font size=2 color=navy face=Arial><span
style='font-size:10.0pt;font-family:Arial;color:navy'>It is undoubtedly very
important to evaluate the genetics of all currently existing cultured
isolates in <st1:country-region w:st="on"><st1:place w:st="on">India</st1:place></st1:country-region>.
To be prudent , clones of these isolates need to be included for examination
as well. Over the years, we have gotten some >300 samples through the
kindness of our colleagues in different forms, ranging from purified DNAs to
live/killed parasites to clinical samples. Sample mix-up does occur in my own
lab, regardless of how meticulous we have tried in handling them. In reality,
with no disrespect to my colleagues, samples received occasionally were not
what we expected apparently due to unintentional errors (most likely of our
own in mislabeling). We subsequently check every sample received by nagt PCR
for RFLP or sequencing.</span></font><o:p></o:p></p>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
<p class=yiv573729320msonormal><font size=2 color=navy face=Arial><span
style='font-size:10.0pt;font-family:Arial;color:navy'>To examine all cultured
isolates circulating in India is an undertaking of great importance that can
be best accomplished by a centralized facility, like ATCC which can guarantee
the identity and quality of the cultures (also for distribution). It will be
great to set up a center of such collection in <st1:country-region w:st="on"><st1:place
w:st="on">India</st1:place></st1:country-region> ? I am working with Dr.
Robert Modestina of ATCC to deposit some of my collection and will be happy
to provide them to any center of Leishmania collection.</span></font><o:p></o:p></p>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
<p class=yiv573729320msonormal><font size=2 color=navy face=Arial><span
style='font-size:10.0pt;font-family:Arial;color:navy'>Many years ago, Sarman
Singh of AIIMS analyzed a small collection of India Leishmania cultures he
gathered from different Indian colleagues and brought to my lab, e. g. Ag83,
RMRI, DD8, UR6 etc, showing their heterogeneity by kDNA/nDNA RFLP analyses.
That particular Ag83 is identical to 3 primary cultures of L donovani Bihar
isolates, whereas the UR6 is I/II allelic hetergeneous L tropica in <i><span
style='font-style:italic'>nagt</span></i> sequence (see Table [1] of L.
odnovani/infantum complex No. 90-93 & Table [2] L tropica No. 49 in the
website: <a
href="http://rosalindfranklin.edu/dnn/portals/24/documents/microbiology/sampleList.pdf"
target="_blank">http://rosalindfranklin.edu/dnn/portals/24/documents/microbiology/sampleList.pdf</a>).
DNAs were all isolated from uncloned, cultured promastigotes. It is difficult
to trace the origin of the old cultures, which must have been passed around
in different labs over the years. Consequently, I can’t say for sure
that the UR6 we examined is identical to yours, for example. This goes with
the information for each isolate provided as well.</span></font><o:p></o:p></p>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
<p class=yiv573729320msonormal><font size=2 color=navy face=Arial><span
style='font-size:10.0pt;font-family:Arial;color:navy'>Leishmania DNAs in the
original sources of clinical, fly and reservoir samples are more difficult to
study, but doable. The potential problems of using cultured promastigotes can
be eliminated by that approach.</span></font><o:p></o:p></p>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
<p class=yiv573729320msonormal><font size=2 color=navy face=Arial><span
style='font-size:10.0pt;font-family:Arial;color:navy'>KP </span></font><o:p></o:p></p>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
<div>
<div class=MsoNormal align=center style='text-align:center'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'>
<hr size=2 width="100%" align=center>
</span></font></div>
<p class=yiv573729320msonormal><b><font size=2 face=Tahoma><span
style='font-size:10.0pt;font-family:Tahoma;font-weight:bold'>From:</span></font></b><font
size=2 face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma'>
Madhumita Manna [mailto:<a href="/mc/compose?to=madhumita.manna09@gmail.com"
target="_blank" ymailto="mailto:madhumita.manna09@gmail.com">madhumita.manna09@gmail.com</a>]
<br>
<b><span style='font-weight:bold'>Sent:</span></b> Friday, June 03, 2011
11:47 PM<br>
<b><span style='font-weight:bold'>To:</span></b> Chang, Kwang-Poo</span></font><o:p></o:p></p>
<div>
<div>
<p class=yiv573729320msonormal><font size=2 face=Tahoma><span
style='font-size:10.0pt;font-family:Tahoma'><br>
<b><span style='font-weight:bold'>Cc:</span></b> <a
href="/mc/compose?to=leish-l@lineu.icb.usp.br" target="_blank"
ymailto="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</a><br>
<b><span style='font-weight:bold'>Subject:</span></b> Re: [Leish-l] inquiry</span></font><o:p></o:p></p>
</div>
</div>
</div>
<div>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>Dear Prof. Chang and all,<o:p></o:p></span></font></p>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>Since my Ph.D time at IICB under Prof. Bhaduri, I am
trying to characterize clinical isolates of KA, PKDL collected mainly from
Bihar and very few from West Bengal, by isozyme, RAPD methods and recently I
have come across one isolate having ITS1 sequence matching with K27. By hsp70
also, the isolate was identified as L.tropica. The isolate was collected from
a patient from <st1:place w:st="on">Bihar</st1:place> and he was suffering
from KA and sensitive to SAG. This is matching with the observations made by
Sacks in 1995 taking isolates from <st1:place w:st="on">Bihar</st1:place>. I
have contacted Dr. Sharma for requesting clinical isolates from his lab to
characterize them by isozymes. In order to divulge the population structure
of the causal agents for Indian KA, isolates from different regions of <st1:country-region
w:st="on"><st1:place w:st="on">India</st1:place></st1:country-region> should
be collected and rigorous typing involving many parameters together, should
be carried forward. <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>Kind regards,<o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>Madhumita <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>on Sat, Jun 4, 2011 at 8:09 AM, Madhumita Manna <<a
href="/mc/compose?to=madhumita.manna09@gmail.com" target="_blank"
ymailto="mailto:madhumita.manna09@gmail.com">madhumita.manna09@gmail.com</a>>
wrote:<o:p></o:p></span></font></p>
<div>
<blockquote style='border:none;border-left:solid #CCCCCC 1.0pt;padding:0in 0in 0in 6.0pt;
margin-left:4.8pt;margin-top:5.0pt;margin-right:0in;margin-bottom:5.0pt'>
<div>
<p class=yiv573729320msonormal style='margin-bottom:12.0pt'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
<div>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>---------- Forwarded message ----------<br>
From: <b><span style='font-weight:bold'>Chang, Kwang-Poo</span></b> <<a
href="/mc/compose?to=KwangPoo.Chang@rosalindfranklin.edu" target="_blank"
ymailto="mailto:KwangPoo.Chang@rosalindfranklin.edu">KwangPoo.Chang@rosalindfranklin.edu</a>><o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>Date: Fri, Jun 3, 2011 at 11:27 PM<br>
Subject: Re: [Leish-l] inquiry<o:p></o:p></span></font></p>
</div>
<div>
<div>
<p class=yiv573729320msonormal style='margin-bottom:12.0pt'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'>To: "Dr.Sunil
Arora" <<a href="/mc/compose?to=skarora_in@yahoo.com" target="_blank"
ymailto="mailto:skarora_in@yahoo.com">skarora_in@yahoo.com</a>>, <a
href="/mc/compose?to=mmukhtar@tropmedicine.org" target="_blank"
ymailto="mailto:mmukhtar@tropmedicine.org">mmukhtar@tropmedicine.org</a>,
Sharman <<a href="/mc/compose?to=nandlals@hotmail.com" target="_blank"
ymailto="mailto:nandlals@hotmail.com">nandlals@hotmail.com</a>>, Petr Volf
<<a href="/mc/compose?to=volf@cesnet.cz" target="_blank"
ymailto="mailto:volf@cesnet.cz">volf@cesnet.cz</a>>, Hiro Goto <<a
href="/mc/compose?to=hgoto@usp.br" target="_blank"
ymailto="mailto:hgoto@usp.br">hgoto@usp.br</a>>, elfadil abass <<a
href="/mc/compose?to=elfadil_abass@yahoo.com" target="_blank"
ymailto="mailto:elfadil_abass@yahoo.com">elfadil_abass@yahoo.com</a>>, <a
href="/mc/compose?to=vishwamohan_katoch@yahoo.co.in" target="_blank"
ymailto="mailto:vishwamohan_katoch@yahoo.co.in">vishwamohan_katoch@yahoo.co.in</a>,
lalit Kant ICMR <<a href="/mc/compose?to=lalitkant@icmr.org.in"
target="_blank" ymailto="mailto:lalitkant@icmr.org.in">lalitkant@icmr.org.in</a>><br>
Cc: <a href="/mc/compose?to=leish-l@lineu.icb.usp.br" target="_blank"
ymailto="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</a><o:p></o:p></span></font></p>
<div>
<div>
<p class=yiv573729320msonormal><font size=2 color=navy face=Arial><span
style='font-size:10.0pt;font-family:Arial;color:navy'>Dear Sunil,</span></font><o:p></o:p></p>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
<p class=yiv573729320msonormal><font size=2 color=navy face=Arial><span
style='font-size:10.0pt;font-family:Arial;color:navy'>Glad to hear from you !
It is good for you (and anyone else) to chip in with different views/valuable
information on the subject under discussion. I wish to say the following:</span></font><o:p></o:p></p>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
<ol start=1 type=1>
<li class=MsoNormal style='color:navy;mso-margin-top-alt:auto;mso-margin-bottom-alt:
auto;mso-list:l6 level1 lfo2'><font size=2 color=navy face=Arial><span
style='font-size:10.0pt;font-family:Arial'>It is important for you and
Dr. Sharma to work together, which is very much in line with the spirit
of WorldLeish.</span></font> <o:p></o:p></li>
</ol>
<p class=yiv573729320msonormal style='margin-left:.25in'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
<ol start=2 type=1>
<li class=MsoNormal style='color:navy;mso-margin-top-alt:auto;mso-margin-bottom-alt:
auto;mso-list:l0 level1 lfo3'><font size=2 color=navy face=Arial><span
style='font-size:10.0pt;font-family:Arial'>Leishmania from some CL
patients along the Sutluj river valley belong to the L donovani/infantum
species complex. As you pointed out, they are refractory to in vitro
cultivation and thus very different from those in <st1:place w:st="on">Bihar</st1:place>.
Splenic aspirates from <st1:PersonName w:st="on">Shyam Sundar</st1:PersonName>’s
VL patients produced exuberant growth as promasigotes in 3N, as I
recalled from my visit to his clinic in the 1980’s (although none
of these promastigotes produces kala-azar in hamsters). This is
well-known (correct me if I should be wrong ?). The genetic sites of
these and other Bihar isolates we examined are different from L infantum
strains studied, including those from <st1:country-region w:st="on">China</st1:country-region>
(Xinjiang, <st1:City w:st="on">Beijing</st1:City>, <st1:State w:st="on">Shandong</st1:State>,
<st1:State w:st="on">Sichuan</st1:State>, <st1:State w:st="on">Gansu</st1:State>),
<st1:country-region w:st="on">Pakistan</st1:country-region>, <st1:country-region
w:st="on">Iran</st1:country-region>, <st1:country-region w:st="on"><st1:place
w:st="on">Turkey</st1:place></st1:country-region> and elsewhere (see
Waki et al. 2007 Eukaryot. Cell 6, 198). Interestingly, the Bihar
isolates is identical to one Sri Lanka CL isolate (DNA kindly provided
by Y Matsumoto, U Tokyo) in nagt sequence, consistent with published
report. Most interestingly, <st1:PersonName w:st="on">Shyam Sundar</st1:PersonName>,
JC Dujardin and their colleagues recently reported the isolation of
Leptomonas from ~7% (according to Shyam) of his <st1:place w:st="on">Bihar</st1:place>
kala-alar samples. This, coupled with the difficulty of in vitro
cultures from clinical samples at least in some cases, underscores the
need to directly examine Leishmania in the original sample sources.</span></font>
<o:p></o:p></li>
</ol>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
<ol start=3 type=1>
<li class=MsoNormal style='color:navy;mso-margin-top-alt:auto;mso-margin-bottom-alt:
auto;mso-list:l7 level1 lfo4'><font size=2 color=navy face=Arial><span
style='font-size:10.0pt;font-family:Arial'>I have followed the
convention of naming the causative agents of Indian and Sudanese
kala-azar/PKDL as L donovani. The gene sequences differ between the two
(we examined) as well as from all infantum isolates (including some
previously typed by others as such). However, this L donovani/infantum
species complex is the most sequence-conserved group, consisting of only
5 nagt genetic variants with limited base substitutions (see Fig. 6[D-4]
in Waki et al.). The sequence divergence can be larger among the
infantum variants than between infantum vs donovani. Analyses of their
whole genomic sequencing may shed light on their true evolutionary
relationships in genetic terms. </span></font><o:p></o:p></li>
</ol>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
<ol start=4 type=1>
<li class=MsoNormal style='color:navy;mso-margin-top-alt:auto;mso-margin-bottom-alt:
auto;mso-list:l5 level1 lfo5'><font size=2 color=navy face=Arial><span
style='font-size:10.0pt;font-family:Arial'>What is the best genetic site
for typing Leishmania isolates ? This is the question I have been asked
repeatedly. My answer to that is try to analyze sequence divergence in
as many sites as possible, preferably the entire genomes. Analyses of
most sites can be informative down to the species complex level.
Interpretation beyond that (to the subspecies complex level, for
example) is often difficult (and thus must remain hypothetical) without
a complete database of all existing Leishmania variants. The database is
incomplete because we are unable to grow parasites from many places and
there are too many endemic sites which have not been explored. </span></font><o:p></o:p></li>
</ol>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
<ol start=5 type=1>
<li class=MsoNormal style='color:navy;mso-margin-top-alt:auto;mso-margin-bottom-alt:
auto;mso-list:l2 level1 lfo6'><font size=2 color=navy face=Arial><span
style='font-size:10.0pt;font-family:Arial'>With regard to the ease of
isolating L infantum for continuous cultivation from clinical, sand fly
and reservoir samples, different investigators differ in their
experience. Dr. Bastien said he has no problem. <st1:PersonName w:st="on">Carlos
Costa</st1:PersonName> also told me that he has no difficulty of
growing L chagasi from his patients in <st1:City w:st="on"><st1:place
w:st="on">Teresina</st1:place></st1:City>. He does have a sizable
collection of hundreds (or thousands ?) of isolates, although I was not
given the actual rate of successful isolation.</span></font> <o:p></o:p></li>
</ol>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
<p class=yiv573729320msonormal><font size=2 color=navy face=Arial><span
style='font-size:10.0pt;font-family:Arial;color:navy'>KP</span></font><o:p></o:p></p>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
<div>
<div class=MsoNormal align=center style='text-align:center'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'>
<hr size=2 width="100%" align=center>
</span></font></div>
<p class=yiv573729320msonormal><b><font size=2 face=Tahoma><span
style='font-size:10.0pt;font-family:Tahoma;font-weight:bold'>From:</span></font></b><font
size=2 face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma'>
Dr.Sunil Arora [mailto:<a href="/mc/compose?to=skarora_in@yahoo.com"
target="_blank" ymailto="mailto:skarora_in@yahoo.com">skarora_in@yahoo.com</a>]
<br>
<b><span style='font-weight:bold'>Sent:</span></b> Thursday, June 02, 2011
11:55 PM<br>
<b><span style='font-weight:bold'>To:</span></b> Chang, Kwang-Poo; <a
href="/mc/compose?to=mmukhtar@tropmedicine.org" target="_blank"
ymailto="mailto:mmukhtar@tropmedicine.org">mmukhtar@tropmedicine.org</a>;
Sharman; Petr Volf; Hiro Goto; elfadil abass; <a
href="/mc/compose?to=vishwamohan_katoch@yahoo.co.in" target="_blank"
ymailto="mailto:vishwamohan_katoch@yahoo.co.in">vishwamohan_katoch@yahoo.co.in</a>;
lalit Kant ICMR </span></font><o:p></o:p></p>
<div>
<p class=yiv573729320msonormal><font size=2 face=Tahoma><span
style='font-size:10.0pt;font-family:Tahoma'><br>
<b><span style='font-weight:bold'>Cc:</span></b> <a
href="/mc/compose?to=leish-l@lineu.icb.usp.br" target="_blank"
ymailto="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</a><br>
<b><span style='font-weight:bold'>Subject:</span></b> Re: [Leish-l] inquiry</span></font><o:p></o:p></p>
</div>
</div>
<p class=yiv573729320msonormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
<div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=2
color=black face=Verdana><span style='font-size:10.0pt;font-family:Verdana;
color:black'>Dear KP and all</span></font><o:p></o:p></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=2
color=black face=Verdana><span style='font-size:10.0pt;font-family:Verdana;
color:black'>I have been reading the exchange of emails regarding
characterisation of leishmania isolates from Himachal in <st1:country-region
w:st="on"><st1:place w:st="on">India</st1:place></st1:country-region> by Dr
Sharma and KP. Actually I have also been associated with that though very
briefly and had a chance to try-n-culture some of isolates sent
by Dr Sharma. The cultures became positive but we failed to establish
good passagable cultures from any of aspirates/biopsy specimen sent by
Dr Sharma, although my lab has been able to establish some good number of
primaray cultures from spleen/BM aspirates of Ld strains in
Chandigarh, although almost all the source patients belonged to
Bihar originally for these. So there is definetely some thing very different
about the leishmania strains that are circulating in that geographical area
of <st1:country-region w:st="on"><st1:place w:st="on">India</st1:place></st1:country-region>.
we also had a chance to use a specific PCR (that was developed in my lab and
can distinguish between an Ld and any cutaneous strain). we found about
40-50% of isolates got Ld specific amplification while others didnot show Ld
genome. So I do believe, there is a mix of Ld and some cutaneous strain
in that area which KP is also refering too. It needs to be investigated
further... I know Dr Sharma is on to such effort soon...</span></font><o:p></o:p></p>
</div>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=2
color=black face=Verdana><span style='font-size:10.0pt;font-family:Verdana;
color:black'>regards</span></font><o:p></o:p></p>
</div>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=2
color=black face=Verdana><span style='font-size:10.0pt;font-family:Verdana;
color:black'>sunil</span></font><o:p></o:p></p>
</div>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><em><i><font
size=2 color="#00007f" face=Verdana><span style='font-size:10.0pt;font-family:
Verdana;color:#00007F'>Dr Sunil K.Arora</span></font></i></em><i><font
size=2 color="#00007f" face=Verdana><span style='font-size:10.0pt;font-family:
Verdana;color:#00007F;font-style:italic'><br>
<em><i><font face=Verdana><span style='font-family:Verdana'>Professor </span></font></i></em><br>
<em><i><font face=Verdana><span style='font-family:Verdana'>In Charge HIV
diagnostic and Disease Monitoring Laboratory</span></font></i></em><br>
<em><i><font face=Verdana><span style='font-family:Verdana'>Department of
Immunopathology</span></font></i></em><br>
<em><i><font face=Verdana><span style='font-family:Verdana'>PGIMER,
Chandigarh-160 012</span></font></i></em><br>
<em><i><font face=Verdana><span style='font-family:Verdana'>Ph.:
0091-172-2755192(Off)</span></font></i></em><br>
<em><i><font face=Verdana><span style='font-family:Verdana'>FAX:
0091-172-2744401, 2745078</span></font></i></em><br>
<em><i><font face=Verdana><span style='font-family:Verdana'>Ph:+91-172-2732863
(Res); 9872866087 (cell)</span></font></i></em><br>
<em><i><font face=Verdana><span style='font-family:Verdana'>email: <a
href="/mc/compose?to=skarora_in@yahoo.com" target="_blank"
ymailto="mailto:skarora_in@yahoo.com">skarora_in@yahoo.com</a>; <a
href="/mc/compose?to=skarorain@gmail.com" target="_blank"
ymailto="mailto:skarorain@gmail.com">skarorain@gmail.com</a></span></font></i></em></span></font></i><o:p></o:p></p>
</div>
<div>
<div>
<p class=yiv573729320msonormal style='margin-bottom:12.0pt;background:white;
background-attachment:scroll;background-position-x:0%;background-position-y:
0%'><b><font size=2 color=black face=Arial><span style='font-size:10.0pt;
font-family:Arial;color:black;font-weight:bold'>From:</span></font></b><font
size=2 color=black face=Arial><span style='font-size:10.0pt;font-family:Arial;
color:black'> "Chang, Kwang-Poo" <<a
href="/mc/compose?to=KwangPoo.Chang@rosalindfranklin.edu" target="_blank"
ymailto="mailto:KwangPoo.Chang@rosalindfranklin.edu">KwangPoo.Chang@rosalindfranklin.edu</a>><br>
<b><span style='font-weight:bold'>To:</span></b> <a
href="/mc/compose?to=mmukhtar@tropmedicine.org" target="_blank"
ymailto="mailto:mmukhtar@tropmedicine.org">mmukhtar@tropmedicine.org</a>;
Sharman <<a href="/mc/compose?to=nandlals@hotmail.com" target="_blank"
ymailto="mailto:nandlals@hotmail.com">nandlals@hotmail.com</a>>; Petr Volf
<<a href="/mc/compose?to=volf@cesnet.cz" target="_blank"
ymailto="mailto:volf@cesnet.cz">volf@cesnet.cz</a>>; Hiro Goto <<a
href="/mc/compose?to=hgoto@usp.br" target="_blank"
ymailto="mailto:hgoto@usp.br">hgoto@usp.br</a>>; elfadil abass <<a
href="/mc/compose?to=elfadil_abass@yahoo.com" target="_blank"
ymailto="mailto:elfadil_abass@yahoo.com">elfadil_abass@yahoo.com</a>>; <a
href="/mc/compose?to=vishwamohan_katoch@yahoo.co.in" target="_blank"
ymailto="mailto:vishwamohan_katoch@yahoo.co.in">vishwamohan_katoch@yahoo.co.in</a>;
lalit Kant ICMR <<a href="/mc/compose?to=lalitkant@icmr.org.in"
target="_blank" ymailto="mailto:lalitkant@icmr.org.in">lalitkant@icmr.org.in</a>>
</span></font><o:p></o:p></p>
<div>
<p class=yiv573729320msonormal><font size=2 color=black face=Arial><span
style='font-size:10.0pt;font-family:Arial;color:black'><br>
<b><span style='font-weight:bold'>Cc:</span></b> <a
href="/mc/compose?to=leish-l@lineu.icb.usp.br" target="_blank"
ymailto="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</a></span></font><o:p></o:p></p>
</div>
<p class=yiv573729320msonormal><b><font size=2 color=black face=Arial><span
style='font-size:10.0pt;font-family:Arial;color:black;font-weight:bold'>Sent:</span></font></b><font
size=2 color=black face=Arial><span style='font-size:10.0pt;font-family:Arial;
color:black'> Friday, June 3, 2011 12:03 AM </span></font><o:p></o:p></p>
<div>
<p class=yiv573729320msonormal><font size=2 color=black face=Arial><span
style='font-size:10.0pt;font-family:Arial;color:black'><br>
<b><span style='font-weight:bold'>Subject:</span></b> Re: [Leish-l] inquiry</span></font><o:p></o:p></p>
</div>
<div>
<div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=2
color=navy face=Arial><span style='font-size:10.0pt;font-family:Arial;
color:navy'>Dear Maowia,</span></font><o:p></o:p></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=2
color=navy face=Arial><span style='font-size:10.0pt;font-family:Arial;
color:navy'>Thanks again for the info.</span></font><o:p></o:p></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=2
color=navy face=Arial><span style='font-size:10.0pt;font-family:Arial;
color:navy'>Are there any record available for the number of successful
isolations versus the total number attempted for a given endemic site ?</span></font><o:p></o:p></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=2
color=navy face=Arial><span style='font-size:10.0pt;font-family:Arial;
color:navy'>KP</span></font><o:p></o:p></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><b><font size=2
color=black face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma;
color:black;font-weight:bold'>From:</span></font></b><font size=2
color=black face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma;
color:black'> Maowia Mukhtar [mailto: <a
href="/mc/compose?to=mmukhtar@tropmedicine.org" target="_blank"
ymailto="mailto:mmukhtar@tropmedicine.org">mmukhtar@tropmedicine.org</a> ] <br>
<b><span style='font-weight:bold'>Sent:</span></b> Wednesday, June 01, 2011
10:41 PM<br>
<b><span style='font-weight:bold'>To:</span></b> Chang, Kwang-Poo; 'Sharman';
'Petr Volf'; 'Hiro Goto'; 'elfadil abass'; <a
href="/mc/compose?to=vishwamohan_katoch@yahoo.co.in" target="_blank"
ymailto="mailto:vishwamohan_katoch@yahoo.co.in">vishwamohan_katoch@yahoo.co.in</a>;
'lalit Kant ICMR' </span></font><o:p></o:p></p>
<div>
<p class=yiv573729320msonormal><font size=2 color=black face=Tahoma><span
style='font-size:10.0pt;font-family:Tahoma;color:black'><br>
<b><span style='font-weight:bold'>Cc:</span></b> <a
href="/mc/compose?to=leish-l@lineu.icb.usp.br" target="_blank"
ymailto="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</a></span></font><o:p></o:p></p>
</div>
<div>
<p class=yiv573729320msonormal><b><font size=2 color=black face=Tahoma><span
style='font-size:10.0pt;font-family:Tahoma;color:black;font-weight:bold'>Subject:</span></font></b><font
size=2 color=black face=Tahoma><span style='font-size:10.0pt;font-family:
Tahoma;color:black'> RE: [Leish-l] inquiry</span></font><o:p></o:p></p>
</div>
</div>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=2
color="#1f497d" face=Calibri><span style='font-size:10.0pt;font-family:Calibri;
color:#1F497D'>Dear KP,</span></font><o:p></o:p></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=2
color="#1f497d" face=Calibri><span style='font-size:10.0pt;font-family:Calibri;
color:#1F497D'>Thanks for your comments and valuable notes. We have a high
success rate inc culturing Leishmania from clinical aspirates. We have even
succeeded in culturing Leishmania directly from PKDL lesions without using
hamsters although the parasite is known to be scarce in these lesions.
We have noticed that the rate of success of cultures varies
between endemic regions in <st1:country-region w:st="on"><st1:place w:st="on">Sudan</st1:place></st1:country-region>
as well as between the clinical forms. Sudanese Leishmania donovani is
known to be different from the Indian donovani even in their response to
antileishmania drugs and in their reaction using different diagnostic
serology tests. </span></font><o:p></o:p></p>
</div>
<div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=2
color="#1f497d" face=Calibri><span style='font-size:10.0pt;font-family:Calibri;
color:#1F497D'>Best regards</span></font><o:p></o:p></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=2
color="#1f497d" face=Calibri><span style='font-size:10.0pt;font-family:Calibri;
color:#1F497D'>Maowia</span></font><o:p></o:p></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><b><font size=2
color="#1f497d" face=Calibri><span style='font-size:10.0pt;font-family:Calibri;
color:#1F497D;font-weight:bold'>Professor Maowia M. Mukhtar</span></font></b><o:p></o:p></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><st1:place w:st="on"><st1:PlaceType
w:st="on"><b><font size=2 color="#1f497d" face=Calibri><span
style='font-size:10.0pt;font-family:Calibri;color:#1F497D;font-weight:bold'>Institute</span></font></b></st1:PlaceType><b><font
size=2 color="#1f497d" face=Calibri><span style='font-size:10.0pt;
font-family:Calibri;color:#1F497D;font-weight:bold'> of <st1:PlaceName
w:st="on">Endemic</st1:PlaceName></span></font></b></st1:place><b><font
size=2 color="#1f497d" face=Calibri><span style='font-size:10.0pt;font-family:
Calibri;color:#1F497D;font-weight:bold'> Diseases</span></font></b><o:p></o:p></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><st1:place w:st="on"><st1:PlaceType
w:st="on"><b><font size=2 color="#1f497d" face=Calibri><span
style='font-size:10.0pt;font-family:Calibri;color:#1F497D;font-weight:bold'>University</span></font></b></st1:PlaceType><b><font
size=2 color="#1f497d" face=Calibri><span style='font-size:10.0pt;
font-family:Calibri;color:#1F497D;font-weight:bold'> of <st1:PlaceName
w:st="on">Khartoum</st1:PlaceName></span></font></b></st1:place><o:p></o:p></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><b><font size=2
color="#1f497d" face=Calibri><span style='font-size:10.0pt;font-family:Calibri;
color:#1F497D;font-weight:bold'>P.O. Box 11463, khartoum, Sudan</span></font></b><o:p></o:p></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><st1:City w:st="on"><st1:place
w:st="on"><b><font size=2 color="#1f497d" face=Calibri><span
style='font-size:10.0pt;font-family:Calibri;color:#1F497D;font-weight:bold'>Mobile</span></font></b></st1:place></st1:City><b><font
size=2 color="#1f497d" face=Calibri><span style='font-size:10.0pt;font-family:
Calibri;color:#1F497D;font-weight:bold'>; +249912234268</span></font></b><o:p></o:p></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><b><font size=2
color="#1f497d" face=Calibri><span style='font-size:10.0pt;font-family:Calibri;
color:#1F497D;font-weight:bold'>Fax: +249183779712</span></font></b><o:p></o:p></p>
</div>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
</div>
<div>
<div style='border:none;border-top:solid #B5C4DF 1.0pt;padding:3.0pt 0in 0in 0in'>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><b><font size=2
color=black face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma;
color:black;font-weight:bold'>From:</span></font></b><font size=2
color=black face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma;
color:black'> Chang, Kwang-Poo [mailto:<a
href="/mc/compose?to=KwangPoo.Chang@rosalindfranklin.edu" target="_blank"
ymailto="mailto:KwangPoo.Chang@rosalindfranklin.edu">KwangPoo.Chang@rosalindfranklin.edu</a>]
<br>
<b><span style='font-weight:bold'>Sent:</span></b> 02 June 2011 01:42<br>
<b><span style='font-weight:bold'>To:</span></b> mmukhtar; Sharman; Petr
Volf; Hiro Goto; elfadil abass; <a
href="/mc/compose?to=vishwamohan_katoch@yahoo.co.in" target="_blank"
ymailto="mailto:vishwamohan_katoch@yahoo.co.in">vishwamohan_katoch@yahoo.co.in</a>;
lalit Kant ICMR<br>
<b><span style='font-weight:bold'>Cc:</span></b> <a
href="/mc/compose?to=leish-l@lineu.icb.usp.br" target="_blank"
ymailto="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</a>;
Chang, Kwang-Poo </span></font><o:p></o:p></p>
<div>
<p class=yiv573729320msonormal><font size=2 color=black face=Tahoma><span
style='font-size:10.0pt;font-family:Tahoma;color:black'><br>
<b><span style='font-weight:bold'>Subject:</span></b> RE: [Leish-l] inquiry</span></font><o:p></o:p></p>
</div>
</div>
</div>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=2
color=navy face=Arial><span style='font-size:10.0pt;font-family:Arial;
color:navy'>Dear Maowia,</span></font><o:p></o:p></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=2
color=navy face=Arial><span style='font-size:10.0pt;font-family:Arial;
color:navy'>Gald to hear from you with your work-in-progress. May I ask you
some questions and share with you some info from my very limited experience
with Sudanese L donovani ? Please correct my statements, if inccorrect.</span></font><o:p></o:p></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<ol start=1 type=1>
<li class=MsoNormal style='color:navy;mso-margin-top-alt:auto;mso-margin-bottom-alt:
auto;mso-list:l1 level1 lfo7;background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=2
color=navy face=Arial><span style='font-size:10.0pt;font-family:Arial'>“difficult
to maintain” means that the promastigotes emerge after incubation
of lesion aspirates/punctures in appropriate media and replicate during
successive passages, but are low in cell density and easily lost during
repetitive passages for continuous cultivation ?</span></font> <o:p></o:p></li>
</ol>
<div style='margin-left:.25in'>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<ol start=2 type=1>
<li class=MsoNormal style='color:navy;mso-margin-top-alt:auto;mso-margin-bottom-alt:
auto;mso-list:l9 level1 lfo8;background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=2
color=navy face=Arial><span style='font-size:10.0pt;font-family:Arial'>“difficult
to grow” means that the promastigotes never emerge or emerge after
incubation of lesion aspirates/punctures in appropriate media, but
subsequently fail to replicate or replicate to a limited extent on
subculture ?</span></font> <o:p></o:p></li>
</ol>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<ol start=3 type=1>
<li class=MsoNormal style='color:navy;mso-margin-top-alt:auto;mso-margin-bottom-alt:
auto;mso-list:l3 level1 lfo9;background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=2
color=navy face=Arial><span style='font-size:10.0pt;font-family:Arial'>What
is the rate of success in your attempts to isolate parasites in culture
from clinical samples in each endemic site over the years ? </span></font><o:p></o:p></li>
</ol>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<ol start=4 type=1>
<li class=MsoNormal style='color:navy;mso-margin-top-alt:auto;mso-margin-bottom-alt:
auto;mso-list:l4 level1 lfo10;background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=2
color=navy face=Arial><span style='font-size:10.0pt;font-family:Arial'>The
Sudanese strain used by many in the <st1:country-region w:st="on"><st1:place
w:st="on">USA</st1:place></st1:country-region> was probably brought
over decades ago by Donald Hyneman and established in Syrian Golden
hamsters by Leslie Stauber and his students. In the 70’s, I had
the good fortune of working with Dennis Dwyer on his S1 or S2 strains of
this origin. As widely reported in the literature, this is an excellent
model of visceral leishmaniasis, producing typical clinical outcome
consistently, including invariable death and splenic LD loads of up to
10<sup>10</sup> per heavily infected spleen. I recall I had difficult
time to differentiate these splenic amastigotes into promastigotes. I
did see some motile promastigotes. While a small number remains viable,
many died and appeared as ghosts during the subsequent incubation. This
has led me to suspect selection of cultivable clones. In some, but not
all of these cultures, promastigotes eventually became ‘adapted’
to the culture conditions and grew, producing consistently up to ~40 X
10<sup>6</sup> cells/ml in 4-5 days in successive passages (starting
from ~10<sup>6</sup>/ml = ~ 4-5 generation or replication cycles). The
stationary phase promastigotes of these cultures no longer produce good infection
of hamsters, irrespective of the passage numbers (Isolation of
‘metacyclics” to produce good infection has since been
reported in the literature, if I remember correctly). These
promastigotes are more similar to the Mediterranean L infantum than to
the Indian L donovani of the <st1:place w:st="on">Bihar</st1:place>
region in the sequence of their single-copy protein-coding genes, as
probably already shown by many based on sequencing other genetic sites. </span></font><o:p></o:p></li>
</ol>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=2
color=navy face=Arial><span style='font-size:10.0pt;font-family:Arial;
color:navy'>KP</span></font><o:p></o:p></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><b><font size=2
color=black face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma;
color:black;font-weight:bold'>From:</span></font></b><font size=2
color=black face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma;
color:black'> Maowia Mukhtar [mailto: <a
href="/mc/compose?to=mmukhtar@tropmedicine.org" target="_blank"
ymailto="mailto:mmukhtar@tropmedicine.org">mmukhtar@tropmedicine.org</a> ] <br>
<b><span style='font-weight:bold'>Sent:</span></b> Tuesday, May 31, 2011 2:30
PM<br>
<b><span style='font-weight:bold'>To:</span></b> 'Sharman'; 'Petr Volf';
Chang, Kwang-Poo; 'Hiro Goto'; 'elfadil abass'; <a
href="/mc/compose?to=vishwamohan_katoch@yahoo.co.in" target="_blank"
ymailto="mailto:vishwamohan_katoch@yahoo.co.in">vishwamohan_katoch@yahoo.co.in</a>;
'lalit Kant ICMR' </span></font><o:p></o:p></p>
<div>
<p class=yiv573729320msonormal><font size=2 color=black face=Tahoma><span
style='font-size:10.0pt;font-family:Tahoma;color:black'><br>
<b><span style='font-weight:bold'>Cc:</span></b> <a
href="/mc/compose?to=leish-l@lineu.icb.usp.br" target="_blank"
ymailto="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</a></span></font><o:p></o:p></p>
</div>
<p class=yiv573729320msonormal><b><font size=2 color=black face=Tahoma><span
style='font-size:10.0pt;font-family:Tahoma;color:black;font-weight:bold'>Subject:</span></font></b><font
size=2 color=black face=Tahoma><span style='font-size:10.0pt;font-family:
Tahoma;color:black'> RE: [Leish-l] inquiry</span></font> <o:p></o:p></p>
</div>
</div>
<div>
<div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=2
color="#1f497d" face=Calibri><span style='font-size:10.0pt;font-family:Calibri;
color:#1F497D'>Dear Colleagues,</span></font><o:p></o:p></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=2
color="#1f497d" face=Calibri><span style='font-size:10.0pt;font-family:Calibri;
color:#1F497D'>We have very interesting experience with Sudanese L.donovani.
This group of parasite although are genetically homogenous they still cause
diverse clinical forms in <st1:country-region w:st="on"><st1:place w:st="on">Sudan</st1:place></st1:country-region>
ranging from VL, PKDL, ML to CL. We have isolated L. donovani MON82
from classic cutaneous ulcers and typed them using both isoenzyme and
molecular typing techniques. Please check our manuscript (Elamin et al. 2008.
</span></font><font size=1 color=black face=Arial><span style='font-size:
9.0pt;font-family:Arial;color:black'>Trans R Soc </span></font><font size=1
color="#1f497d" face=Arial><span style='font-size:9.0pt;font-family:Arial;
color:#1F497D'>Trop Med Hyg. 2008 Jan;102(1):54-7.). We have analyzed several
virulence genes comparing the isolates from the diverse clinical forms and
they turned to be are highly homologous. Immunologically they induce
different immune responses (TH1/TH2). We know the vector of L.donovani VL
isolates which is P. orientalis, but we still don’t know the vector of
cutaneous L. donovani parasite. We believe the vector plays an important role
in the pathogenesis and the outcome of L. donovani infection together with
the host immune response and may be some of the parasite virulence factors.
We will soon published our data on parasite typing and host immune responses
of the diverse clinical forms. </span></font><o:p></o:p></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=1
color="#1f497d" face=Arial><span style='font-size:9.0pt;font-family:Arial;
color:#1F497D'>Best regards</span></font><o:p></o:p></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=1
color="#1f497d" face=Arial><span style='font-size:9.0pt;font-family:Arial;
color:#1F497D'>Maowia</span></font><o:p></o:p></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><b><font size=2
color="#1f497d" face=Calibri><span style='font-size:10.0pt;font-family:Calibri;
color:#1F497D;font-weight:bold'>Professor Maowia M. Mukhtar</span></font></b><o:p></o:p></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><st1:place w:st="on"><st1:PlaceType
w:st="on"><b><font size=2 color="#1f497d" face=Calibri><span
style='font-size:10.0pt;font-family:Calibri;color:#1F497D;font-weight:bold'>Institute</span></font></b></st1:PlaceType><b><font
size=2 color="#1f497d" face=Calibri><span style='font-size:10.0pt;
font-family:Calibri;color:#1F497D;font-weight:bold'> of <st1:PlaceName
w:st="on">Endemic</st1:PlaceName></span></font></b></st1:place><b><font
size=2 color="#1f497d" face=Calibri><span style='font-size:10.0pt;font-family:
Calibri;color:#1F497D;font-weight:bold'> Diseases</span></font></b><o:p></o:p></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><st1:place w:st="on"><st1:PlaceType
w:st="on"><b><font size=2 color="#1f497d" face=Calibri><span
style='font-size:10.0pt;font-family:Calibri;color:#1F497D;font-weight:bold'>University</span></font></b></st1:PlaceType><b><font
size=2 color="#1f497d" face=Calibri><span style='font-size:10.0pt;
font-family:Calibri;color:#1F497D;font-weight:bold'> of <st1:PlaceName
w:st="on">Khartoum</st1:PlaceName></span></font></b></st1:place><o:p></o:p></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><b><font size=2
color="#1f497d" face=Calibri><span style='font-size:10.0pt;font-family:Calibri;
color:#1F497D;font-weight:bold'>P.O. Box 11463, khartoum, Sudan</span></font></b><o:p></o:p></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><st1:City w:st="on"><st1:place
w:st="on"><b><font size=2 color="#1f497d" face=Calibri><span
style='font-size:10.0pt;font-family:Calibri;color:#1F497D;font-weight:bold'>Mobile</span></font></b></st1:place></st1:City><b><font
size=2 color="#1f497d" face=Calibri><span style='font-size:10.0pt;font-family:
Calibri;color:#1F497D;font-weight:bold'>; +249912234268</span></font></b><o:p></o:p></p>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><b><font size=2
color="#1f497d" face=Calibri><span style='font-size:10.0pt;font-family:Calibri;
color:#1F497D;font-weight:bold'>Fax: +249183779712</span></font></b><o:p></o:p></p>
</div>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<div style='border:none;border-top:solid #B5C4DF 1.0pt;padding:3.0pt 0in 0in 0in'>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><b><font size=2
color=black face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma;
color:black;font-weight:bold'>From:</span></font></b><font size=2
color=black face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma;
color:black'> <a href="/mc/compose?to=leish-l-bounces@lineu.icb.usp.br"
target="_blank" ymailto="mailto:leish-l-bounces@lineu.icb.usp.br">leish-l-bounces@lineu.icb.usp.br</a>
[mailto:<a href="/mc/compose?to=leish-l-bounces@lineu.icb.usp.br"
target="_blank" ymailto="mailto:leish-l-bounces@lineu.icb.usp.br">leish-l-bounces@lineu.icb.usp.br</a>]
<b><span style='font-weight:bold'>On Behalf Of </span></b>Sharman<br>
<b><span style='font-weight:bold'>Sent:</span></b> 31 May 2011 18:13<br>
<b><span style='font-weight:bold'>To:</span></b> Petr Volf; Chang, Kwang-Poo;
Hiro Goto; elfadil abass; <a
href="/mc/compose?to=vishwamohan_katoch@yahoo.co.in" target="_blank"
ymailto="mailto:vishwamohan_katoch@yahoo.co.in">vishwamohan_katoch@yahoo.co.in</a>;
lalit Kant ICMR<br>
<b><span style='font-weight:bold'>Cc:</span></b> <a
href="/mc/compose?to=leish-l@lineu.icb.usp.br" target="_blank"
ymailto="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</a><br>
<b><span style='font-weight:bold'>Subject:</span></b> Re: [Leish-l] inquiry</span></font><o:p></o:p></p>
</div>
</div>
</div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
color=black face=Calibri><span style='font-size:12.0pt;font-family:Calibri;
color:black'>Hi Petr,</span></font><o:p></o:p></p>
</div>
</div>
<div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
color=black face=Calibri><span style='font-size:12.0pt;font-family:Calibri;
color:black'>Thanks for enclosing the pdf. In fact the strain which we are
dealing with is not very difficult to grow but difficult to maintain, It is
rK 39 positive and as I mentioned in the conversation below that even the
serous exudate from the ulcer is also positive for rK 39 dipstick. In between
because of my relocation to a different institute I was little away from this
work but I intend to resume this work in couple of months. Although in past
we tried to isolate the parasite from sand flies but did not succeed. We
will surely attempt again with the techniques described by you and others.
Contamination is the problem.</span></font><o:p></o:p></p>
</div>
</div>
<div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
color=black face=Calibri><span style='font-size:12.0pt;font-family:Calibri;
color:black'>This particular focus seems to be a complex one.</span></font><o:p></o:p></p>
</div>
</div>
<div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
color=black face=Calibri><span style='font-size:12.0pt;font-family:Calibri;
color:black'>NL Sharma</span></font><font color=black><span style='color:
black'> </span></font><o:p></o:p></p>
</div>
</div>
<div>
<div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
</div>
<div>
<div>
<div>
<p class=yiv573729320msonormal style='background:whitesmoke;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><b><font size=2
color=black face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma;
color:black;font-weight:bold'>From:</span></font></b><font size=2
color=black face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma;
color:black'> <a href="/mc/compose?to=volf@cesnet.cz" target="_blank"
title="volf@cesnet.cz" ymailto="mailto:volf@cesnet.cz">Petr Volf</a> </span></font><o:p></o:p></p>
</div>
</div>
<div>
<div>
<p class=yiv573729320msonormal style='background:whitesmoke;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><b><font size=2
color=black face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma;
color:black;font-weight:bold'>Sent:</span></font></b><font size=2
color=black face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma;
color:black'> Tuesday, May 31, 2011 12:36 PM</span></font><o:p></o:p></p>
</div>
</div>
<div>
<div>
<p class=yiv573729320msonormal style='background:whitesmoke;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><b><font size=2
color=black face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma;
color:black;font-weight:bold'>To:</span></font></b><font size=2 color=black
face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma;color:black'> <a
href="/mc/compose?to=KwangPoo.Chang@rosalindfranklin.edu" target="_blank"
title="KwangPoo.Chang@rosalindfranklin.edu"
ymailto="mailto:KwangPoo.Chang@rosalindfranklin.edu">Chang, Kwang-Poo</a> ; <a
href="/mc/compose?to=nandlals@hotmail.com" target="_blank"
title="mailto:nandlals@hotmail.com CTRL + Click to follow link"
ymailto="mailto:nandlals@hotmail.com">Sharman</a> ; <a
href="/mc/compose?to=hgoto@usp.br" target="_blank"
title="mailto:hgoto@usp.br CTRL + Click to follow link"
ymailto="mailto:hgoto@usp.br">Hiro Goto</a> ; <a
href="/mc/compose?to=elfadil_abass@yahoo.com" target="_blank"
title="mailto:elfadil_abass@yahoo.com CTRL + Click to follow link"
ymailto="mailto:elfadil_abass@yahoo.com">elfadil abass</a> </span></font><o:p></o:p></p>
</div>
</div>
<div>
<div>
<p class=yiv573729320msonormal style='background:whitesmoke;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><b><font size=2
color=black face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma;
color:black;font-weight:bold'>Cc:</span></font></b><font size=2 color=black
face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma;color:black'> <a
href="/mc/compose?to=leish-l@lineu.icb.usp.br" target="_blank"
title="leish-l@lineu.icb.usp.br" ymailto="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</a>
</span></font><o:p></o:p></p>
</div>
</div>
<div>
<div>
<p class=yiv573729320msonormal style='background:whitesmoke;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><b><font size=2
color=black face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma;
color:black;font-weight:bold'>Subject:</span></font></b><font size=2
color=black face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma;
color:black'> Re: [Leish-l] inquiry</span></font><o:p></o:p></p>
</div>
</div>
</div>
</div>
<div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
</div>
<div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=2
color=black face=Arial><span style='font-size:10.0pt;font-family:Arial;
color:black'>Hi K.P.,</span></font><o:p></o:p></p>
</div>
</div>
<div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=2
color=black face=Arial><span style='font-size:10.0pt;font-family:Arial;
color:black'>in Cukurova region, <st1:country-region w:st="on"><st1:place
w:st="on">Turkey</st1:place></st1:country-region> , cutaneous L. infantum
(now it seems that we are dealing with L.donovani/L. infantum hybrid)
grew very poorely if we isolated them from patients. Only 1 of 25 isolations
was succesfull. However, the same strain (confirmed by molecular methods)
grew repeatedly and very well if we isolated them from sandflies. It might be
useful for Dr. Sharma try to get isolates from sand flies. It is very
laborious but very useful, in Cukurova we got about dozen of isolates by this
method (all identical).</span></font><o:p></o:p></p>
</div>
</div>
<div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=2
color=black face=Arial><span style='font-size:10.0pt;font-family:Arial;
color:black'>Patients are rK39 negative, see attached paper.</span></font><o:p></o:p></p>
</div>
</div>
<div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=2
color=black face=Arial><span style='font-size:10.0pt;font-family:Arial;
color:black'>Best wishes</span></font><o:p></o:p></p>
</div>
</div>
<div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=2
color=black face=Arial><span style='font-size:10.0pt;font-family:Arial;
color:black'>Petr</span></font><o:p></o:p></p>
</div>
</div>
<blockquote style='border:none;border-left:solid black 1.5pt;padding:0in 0in 0in 4.0pt;
margin-left:3.75pt;margin-top:5.0pt;margin-right:0in;margin-bottom:5.0pt'>
<div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=2
color=black face=Arial><span style='font-size:10.0pt;font-family:Arial;
color:black'>----- Original Message ----- </span></font><o:p></o:p></p>
</div>
</div>
<div>
<div>
<p class=yiv573729320msonormal style='background:#E4E4E4;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><b><font size=2
color=black face=Arial><span style='font-size:10.0pt;font-family:Arial;
color:black;font-weight:bold'>From:</span></font></b><font size=2
color=black face=Arial><span style='font-size:10.0pt;font-family:Arial;
color:black'> <a href="/mc/compose?to=KwangPoo.Chang@rosalindfranklin.edu"
target="_blank" title="KwangPoo.Chang@rosalindfranklin.edu"
ymailto="mailto:KwangPoo.Chang@rosalindfranklin.edu">Chang, Kwang-Poo</a> </span></font><o:p></o:p></p>
</div>
</div>
<div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><b><font size=2
color=black face=Arial><span style='font-size:10.0pt;font-family:Arial;
color:black;font-weight:bold'>To:</span></font></b><font size=2 color=black
face=Arial><span style='font-size:10.0pt;font-family:Arial;color:black'> <a
href="/mc/compose?to=nandlals@hotmail.com" target="_blank"
title="nandlals@hotmail.com" ymailto="mailto:nandlals@hotmail.com">Sharman</a>
; <a href="/mc/compose?to=hgoto@usp.br" target="_blank" title="hgoto@usp.br"
ymailto="mailto:hgoto@usp.br">Hiro Goto</a> ; <a
href="/mc/compose?to=elfadil_abass@yahoo.com" target="_blank"
title="elfadil_abass@yahoo.com" ymailto="mailto:elfadil_abass@yahoo.com">elfadil
abass</a> </span></font><o:p></o:p></p>
</div>
</div>
<div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><b><font size=2
color=black face=Arial><span style='font-size:10.0pt;font-family:Arial;
color:black;font-weight:bold'>Cc:</span></font></b><font size=2 color=black
face=Arial><span style='font-size:10.0pt;font-family:Arial;color:black'> <a
href="/mc/compose?to=leish-l@lineu.icb.usp.br" target="_blank"
title="leish-l@lineu.icb.usp.br" ymailto="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</a>
</span></font><o:p></o:p></p>
</div>
</div>
<div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><b><font size=2
color=black face=Arial><span style='font-size:10.0pt;font-family:Arial;
color:black;font-weight:bold'>Sent:</span></font></b><font size=2
color=black face=Arial><span style='font-size:10.0pt;font-family:Arial;
color:black'> Sunday, May 22, 2011 1:39 AM</span></font><o:p></o:p></p>
</div>
</div>
<div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><b><font size=2
color=black face=Arial><span style='font-size:10.0pt;font-family:Arial;
color:black;font-weight:bold'>Subject:</span></font></b><font size=2
color=black face=Arial><span style='font-size:10.0pt;font-family:Arial;
color:black'> Re: [Leish-l] inquiry</span></font><o:p></o:p></p>
</div>
</div>
<div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
</div>
<div>
<div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=2
color=black face=Arial><span style='font-size:10.0pt;font-family:Arial;
color:black'>Dr. NL Sharma has been working on an important CL endemic area
along the <st1:PlaceName w:st="on">Satluj</st1:PlaceName> <st1:PlaceType
w:st="on">River</st1:PlaceType> valley to the south of Himalaya in <st1:country-region
w:st="on"><st1:place w:st="on">India</st1:place></st1:country-region> (Please
correct me should I be wrong for anything I said here and below). I had the
good fortune of visiting the site several years back courtesy of Dr. Sharma. </span></font><o:p></o:p></p>
</div>
</div>
<div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
</div>
<div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=2
color=black face=Arial><span style='font-size:10.0pt;font-family:Arial;
color:black'>I believe Dr. Sharma's finding is important, since the parasites
there are different from the familiar Indian L donovani in <st1:place w:st="on">Bihar</st1:place>
. The parasites are refratory to in vitro cultivation. They do differentiate
into promastigotes and may grow a little, but can't really be
subcultured to establish passageable lines. This is very much reminescent of
L infantum in the Mediterranean area. I recall Dr. Sharma has also found
rK39+ dogs, if I remember correctly. If so, Satluj river valley is
endemic to the infantile CL.</span></font><o:p></o:p></p>
</div>
</div>
<div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
</div>
<div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=2
color=black face=Arial><span style='font-size:10.0pt;font-family:Arial;
color:black'>Analyses of several batches of basically clinical CL samples
from Dr. Sharma showed evidence of L infantum, but also L tropica as well as
a mixture of the two in one sample. This is the same picture we have
noted for samples from <st1:place w:st="on"><st1:City w:st="on">Hatay</st1:City>
, <st1:country-region w:st="on">Turkey</st1:country-region></st1:place> .</span></font><o:p></o:p></p>
</div>
</div>
<div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
</div>
<div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
color=black face=Arial><span style='font-size:12.0pt;font-family:Arial;
color:black'>These observations make me wonder a lot about our current
knowledge on the clinico-epidemiology based on data collected previously by
analyses of cultured promastigotes from one or
few 'representative samples'. Nowaday, technology makes it very
doable to work with biological samples for Leish DNAs directly from sand
flies, patients and reservoir animals.</span></font><o:p></o:p></p>
</div>
</div>
<div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
</div>
<div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=2
color=black face=Arial><span style='font-size:10.0pt;font-family:Arial;
color:black'>KP</span></font><o:p></o:p></p>
</div>
</div>
<div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
</div>
</div>
<div>
<div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
</div>
<div>
<div style='margin-bottom:12.0pt'>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><b><font size=2
color=black face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma;
color:black;font-weight:bold'>From:</span></font></b><font size=2
color=black face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma;
color:black'> <a href="/mc/compose?to=leish-l-bounces@lineu.icb.usp.br"
target="_blank" ymailto="mailto:leish-l-bounces@lineu.icb.usp.br">leish-l-bounces@lineu.icb.usp.br</a>
on behalf of Sharman<br>
<b><span style='font-weight:bold'>Sent:</span></b> Fri 5/20/2011 11:31 PM<br>
<b><span style='font-weight:bold'>To:</span></b> Hiro Goto; elfadil abass<br>
<b><span style='font-weight:bold'>Cc:</span></b> <a
href="/mc/compose?to=leish-l@lineu.icb.usp.br" target="_blank"
ymailto="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</a><br>
<b><span style='font-weight:bold'>Subject:</span></b> Re: [Leish-l] inquiry</span></font><o:p></o:p></p>
</div>
</div>
</div>
<div>
<div>
<p class=yiv573729320msonormal style='background:white;background-attachment:
scroll;background-position-x:0%;background-position-y:0%'><font size=2
color=black face="Times New Roman"><span style='font-size:10.0pt;color:black'>Dear
all<br>
I agree with Hiro gito, We are working on a focus where the CL is<br>
predominantly caused by L. donovani, and the rK 39 STRIPS GIVE POSITIVE<br>
RESULTS WITH SERA as well as serous exudate from the lesion. The results are<br>
dependent upon species.<br>
NL Sharma<br>
<br>
--------------------------------------------------<br>
From: "Hiro Goto" <<a href="/mc/compose?to=hgoto@usp.br"
target="_blank" ymailto="mailto:hgoto@usp.br">hgoto@usp.br</a>><br>
Sent: Wednesday, May 18, 2011 12:09 AM<br>
To: "elfadil abass" <<a
href="/mc/compose?to=elfadil_abass@yahoo.com" target="_blank"
ymailto="mailto:elfadil_abass@yahoo.com">elfadil_abass@yahoo.com</a>><br>
Cc: <<a href="/mc/compose?to=leish-l@lineu.icb.usp.br" target="_blank"
ymailto="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</a>><br>
Subject: Re: [Leish-l] inquiry<br>
<br>
> Dear all,<br>
> In our oppinion, DAT and rK39 for those samples are not indicated
since<br>
> these tests are produced for the diagnosis of visceral
leishmaniasis.<br>
> In case of tegumentary leishmaniasis, it is very appropriate the<br>
> observation of J.J. Shaw appointing species specificity of antibody<br>
> response in these cases. Titers of antibodies are in general low
in these<br>
> cases therefore depending on the species, it may result negative.
We have<br>
> published a review recently in Expert Rev. Anti Infect. Ther.
8(4),<br>
> 419?433 (2010), Current diagnosis and treatment of cutaneous and<br>
> mucocutaneous leishmaniasis, where we raise this point.<br>
> Hiro Goto<br>
><br>
> Citando elfadil abass <<a
href="/mc/compose?to=elfadil_abass@yahoo.com" target="_blank"
ymailto="mailto:elfadil_abass@yahoo.com">elfadil_abass@yahoo.com</a>>:<br>
><br>
>> Dear all I would recommend using DAT and rK39 strip test to measure<br>
>> antibody<br>
>> responses and to evaluate the diagnostic efficiency for both
tests in<br>
>> such group<br>
>> of patients.<br>
>><br>
>> Elfadil Abass<br>
>>
________________________________________________________________________________<br>
>> <st1:place w:st="on"><st1:PlaceType w:st="on">Institute</st1:PlaceType>
of <st1:PlaceName w:st="on">Medical</st1:PlaceName></st1:place> Microbiology
and Hospital Hygiene<br>
>> <st1:PlaceName w:st="on">Philipps</st1:PlaceName> <st1:PlaceName
w:st="on">University</st1:PlaceName> <st1:City w:st="on"><st1:place w:st="on">Marburg</st1:place></st1:City><br>
>> BMFZ / Hans-Meerwein Straße 2<br>
>> D-35033 <st1:place w:st="on"><st1:City w:st="on">Marburg</st1:City>
, <st1:country-region w:st="on">Germany</st1:country-region></st1:place><br>
>>
________________________________________________________________________________<br>
>><br>
>><br>
>><br>
>><br>
>> ________________________________<br>
>> From: Nuha Nuwayri-Salti <<a
href="/mc/compose?to=racha@aub.edu.lb" target="_blank"
ymailto="mailto:racha@aub.edu.lb">racha@aub.edu.lb</a>><br>
>> To: saad saad <<a href="/mc/compose?to=saad1426@gmail.com"
target="_blank" ymailto="mailto:saad1426@gmail.com">saad1426@gmail.com</a>>;
"<a href="/mc/compose?to=leish-l@lineu.icb.usp.br" target="_blank"
ymailto="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</a>"<br>
>> <<a href="/mc/compose?to=leish-l@lineu.icb.usp.br" target="_blank"
ymailto="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</a>><br>
>> Sent: Fri, May 13, 2011 9:39:33 AM<br>
>> Subject: Re: [Leish-l] inquiry<br>
>><br>
>> Dear Saad first precaution to take is to separate your samples into<br>
>> several<br>
>> portions each (at least 5 each being no more than a few
hundred(200-400)<br>
>> microliters). This is a necessary precaution to avoid freezing and<br>
>> thawing<br>
>> several times the same sample which will be the case should you do<br>
>> different<br>
>> studies at different times which is unavoidable.<br>
>><br>
>> What you can do is correlate the type(ulcerated, abscess, furuncle<br>
>> etc.. ) the<br>
>> locale, the number and age of lesions with the levels of antibody
in the<br>
>> sera of<br>
>> these patgients and also monitor cell mediated immunity with
leishmanin<br>
>> skin<br>
>> test!<br>
>> I have just published (in print) an article about having circulating<br>
>> parasites<br>
>> in some of these patients with apparently pure cutaneous disease. It
is<br>
>> the<br>
>> first paper that revealed this fact. It would be interesting to
confirm<br>
>> or<br>
>> de-confirm this fact repeating what we did.<br>
>> Best wishes<br>
>><br>
>> Nuha Nuwayri-Salti MD<br>
>><br>
>><br>
>><br>
>> AOA<br>
>> Medical Honor Society<br>
>><br>
>><br>
>><br>
>><br>
>> -----Original Message-----<br>
>> From: <a href="/mc/compose?to=leish-l-bounces@lineu.icb.usp.br"
target="_blank" ymailto="mailto:leish-l-bounces@lineu.icb.usp.br">leish-l-bounces@lineu.icb.usp.br</a><br>
>> [<a href="/mc/compose?to=leish-l-bounces@lineu.icb.usp.br"
target="_blank" ymailto="mailto:leish-l-bounces@lineu.icb.usp.br">mailto:leish-l-bounces@lineu.icb.usp.br</a>]<br>
>> On Behalf Of saad saad<br>
>> Sent: Friday, April 29, 2011 11:23 PM<br>
>> To: <a href="/mc/compose?to=leish-l@lineu.icb.usp.br" target="_blank"
ymailto="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</a><br>
>> Subject: [Leish-l] inquiry<br>
>><br>
>> Dear all<br>
>> Hi. i am Saad from <st1:country-region w:st="on"><st1:place w:st="on">Saudi
Arabia</st1:place></st1:country-region> . i have 60 sera from positive case
of<br>
>> CL from south west of the country. I would like to have your<br>
>> recommendations to start a good research line in CL using these
sera.<br>
>> Thanks in advance for your help<br>
>> _______________________________________________<br>
>> Leish-l mailing list<br>
>> <a href="/mc/compose?to=Leish-l@lineu.icb.usp.br" target="_blank"
ymailto="mailto:Leish-l@lineu.icb.usp.br">Leish-l@lineu.icb.usp.br</a><br>
>> <a href="http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l"
target="_blank">http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l</a><br>
>> _______________________________________________<br>
>> Leish-l mailing list<br>
>> <a href="/mc/compose?to=Leish-l@lineu.icb.usp.br" target="_blank"
ymailto="mailto:Leish-l@lineu.icb.usp.br">Leish-l@lineu.icb.usp.br</a><br>
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