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</o:shapelayout></xml><![endif]--></head><body bgcolor=white lang=NL-BE link=blue vlink=blue><div class=WordSection1><p class=MsoNormal><span lang=EN-US style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'>Dear all, I react only now after seeing a lot of mails passing. We are currently undertaking a major study on genetic diversity of L.donovani in the Indian sub-continent (including full genome sequencing). While most of the strains analyzed so far support the post-DDT bottleneck hypothesis (clonal expansion with low genetic homogeneity), we start finding rare genotypes which are very different from the major population. If you are interested, we might include your strains in our analysis.<o:p></o:p></span></p><p class=MsoNormal><span lang=EN-US style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'>Best regards<o:p></o:p></span></p><p class=MsoNormal><span lang=EN-US style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'>JC<o:p></o:p></span></p><p class=MsoNormal><span lang=EN-US style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'><o:p> </o:p></span></p><div><p class=MsoNormal><span lang=EN-US style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'>Prof. Dr Jean-Claude Dujardin<o:p></o:p></span></p><p class=MsoNormal><span lang=EN-US style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'>Head of Molecular Parasitology<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'>Instituut voor Tropische Geneeskunde<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'>Nationalestraat, 155<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'>B-2000 Antwerpen<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'>Belgium<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'>Tel. 32.3.2476358 or 355<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'>Fax. 32.3.2476359<o:p></o:p></span></p></div><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'><o:p> </o:p></span></p><div><div style='border:none;border-top:solid #B5C4DF 1.0pt;padding:3.0pt 0cm 0cm 0cm'><p class=MsoNormal><b><span lang=EN-US style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'>From:</span></b><span lang=EN-US style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'> leish-l-bounces@lineu.icb.usp.br [mailto:leish-l-bounces@lineu.icb.usp.br] <b>On Behalf Of </b>Raj<br><b>Sent:</b> donderdag 2 juni 2011 8:16<br><b>To:</b> nandlals@hotmail.com; Chang, Kwang-Poo; leish-l@lineu.icb.usp.br<br><b>Subject:</b> Re: [Leish-l] cutaneous form of leish in Indian region<o:p></o:p></span></p></div></div><p class=MsoNormal><o:p> </o:p></p><div><p class=MsoNormal><span lang=EN-US style='font-size:10.0pt;font-family:"Arial","sans-serif"'>Hi KP,</span><span lang=EN-US><o:p></o:p></span></p></div><div><p class=MsoNormal><span lang=EN-US style='font-size:10.0pt;font-family:"Arial","sans-serif"'>Certainly I will do. Please suggest Dr NL Sharma to do so.</span><span lang=EN-US><o:p></o:p></span></p></div><div><p class=MsoNormal><span lang=EN-US style='font-size:10.0pt;font-family:"Arial","sans-serif"'>Raj</span><span lang=EN-US><o:p></o:p></span></p></div><blockquote style='border:none;border-left:solid black 1.5pt;padding:0cm 0cm 0cm 4.0pt;margin-left:3.75pt;margin-top:5.0pt;margin-right:0cm;margin-bottom:5.0pt'><div><p class=MsoNormal><span lang=EN-US style='font-size:10.0pt;font-family:"Arial","sans-serif"'>----- Original Message ----- <o:p></o:p></span></p></div><div><p class=MsoNormal style='background:#E4E4E4'><b><span lang=EN-US style='font-size:10.0pt;font-family:"Arial","sans-serif"'>From:</span></b><span lang=EN-US style='font-size:10.0pt;font-family:"Arial","sans-serif"'> <a href="mailto:KwangPoo.Chang@rosalindfranklin.edu" title="KwangPoo.Chang@rosalindfranklin.edu">Chang, Kwang-Poo</a> <o:p></o:p></span></p></div><div><p class=MsoNormal><b><span lang=EN-US style='font-size:10.0pt;font-family:"Arial","sans-serif"'>To:</span></b><span lang=EN-US style='font-size:10.0pt;font-family:"Arial","sans-serif"'> <a href="mailto:raj@cellabs.com.au" title="raj@cellabs.com.au">Raj</a> ; <a href="mailto:hanafi.hanafi.ctr.eg@med.navy.mil" title="hanafi.hanafi.ctr.eg@med.navy.mil">Hanafi, Hanafi CTR EG NAMRU3 </a>; <a href="mailto:nandlals@hotmail.com" title="nandlals@hotmail.com">nandlals@hotmail.com</a> ; <a href="mailto:leish-l@lineu.icb.usp.br" title="leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</a> <o:p></o:p></span></p></div><div><p class=MsoNormal><b><span lang=EN-US style='font-size:10.0pt;font-family:"Arial","sans-serif"'>Sent:</span></b><span lang=EN-US style='font-size:10.0pt;font-family:"Arial","sans-serif"'> Thursday, June 02, 2011 8:41 AM<o:p></o:p></span></p></div><div><p class=MsoNormal><b><span lang=EN-US style='font-size:10.0pt;font-family:"Arial","sans-serif"'>Subject:</span></b><span lang=EN-US style='font-size:10.0pt;font-family:"Arial","sans-serif"'> RE: cutaneous form of leish in Indian region<o:p></o:p></span></p></div><div><p class=MsoNormal><span lang=EN-US><o:p> </o:p></span></p></div><p class=MsoNormal><span lang=EN-US style='font-size:10.0pt;font-family:"Arial","sans-serif";color:navy'>Great if Dr. Sharma would be able to send serum samples to you for evaluating your species-specific exo-antigens.<o:p></o:p></span></p><p class=MsoNormal><span lang=EN-US style='font-size:10.0pt;font-family:"Arial","sans-serif";color:navy'><o:p> </o:p></span></p><p class=MsoNormal><span lang=EN-US style='font-size:10.0pt;font-family:"Arial","sans-serif";color:navy'>KP<o:p></o:p></span></p><p class=MsoNormal><span lang=EN-US style='font-size:10.0pt;font-family:"Arial","sans-serif";color:navy'><o:p> </o:p></span></p><div><div class=MsoNormal align=center style='text-align:center'><span lang=EN-US><hr size=2 width="100%" align=center></span></div><p class=MsoNormal><b><span lang=EN-US style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'>From:</span></b><span lang=EN-US style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'> Raj [mailto:raj@cellabs.com.au] <br><b>Sent:</b> Tuesday, May 31, 2011 9:18 PM<br><b>To:</b> Hanafi, Hanafi CTR EG NAMRU3 ; Chang, Kwang-Poo; <a href="mailto:nandlals@hotmail.com">nandlals@hotmail.com</a>; <a href="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</a><br><b>Subject:</b> cutaneous form of leish in Indian region</span><span lang=EN-US><o:p></o:p></span></p></div><p class=MsoNormal><span lang=EN-US><o:p> </o:p></span></p><div><div><p class=MsoNormal><span lang=EN-US style='font-size:10.0pt;font-family:"Arial","sans-serif"'>G'Day Dr Hanafi,<o:p></o:p></span></p></div><div><p class=MsoNormal><span lang=EN-US style='font-size:10.0pt;font-family:"Arial","sans-serif"'>My enquiry was to know whether <em><span style='font-family:"Arial","sans-serif"'>L. donovani</span></em> in India produces a cutaneous form of lesion or not (I am not referring to PKDL form). There are many reports from SriLanka about the cutaneous manifestations by <em><span style='font-family:"Arial","sans-serif"'>L. donovani</span></em>. The PKDL although is a cutaneous response appears very late in the infection process and this is ascribed to be a protective response against the parasite. <o:p></o:p></span></p></div><div><p class=MsoNormal><span lang=EN-US style='font-size:10.0pt;font-family:"Arial","sans-serif"'> <o:p></o:p></span></p></div><div><p class=MsoNormal><span lang=EN-US style='font-size:10.0pt;font-family:"Arial","sans-serif"'>Prof Chang mentioned <o:p></o:p></span></p></div><div><p class=MsoNormal><span lang=EN-US style='font-size:10.0pt;font-family:"Arial","sans-serif"'>"Analyses of several batches of basically clinical CL samples from Dr. Sharma showed evidence of L infantum, but also L tropica as well as a mixture of the two in one sample. This is the same picture we have noted for samples from Hatay, Turkey." This is from Sutlej region." It means this cutaneous complex requires some investigation. <o:p></o:p></span></p></div><div><p class=MsoNormal><span lang=EN-US style='font-size:10.0pt;font-family:"Arial","sans-serif"'> <o:p></o:p></span></p></div><div><p class=MsoNormal><span lang=EN-US style='font-size:10.0pt;font-family:"Arial","sans-serif"'>I raise species-specific exo-antigens from <em><span style='font-family:"Arial","sans-serif"'>L. tropica</span></em> and <em><span style='font-family:"Arial","sans-serif"'>L infantum</span></em> and I am prepared to test the serum /plasma samples for presence of antibodies. If Dr NL Sharma is prepared to send me specimens as well as clinical plasma or serum samples I would be interested to investigate the antibody response in these situations. <o:p></o:p></span></p></div><div><p class=MsoNormal><span lang=EN-US style='font-size:10.0pt;font-family:"Arial","sans-serif"'> <o:p></o:p></span></p></div><div><p class=MsoNormal><span lang=EN-US style='font-size:10.0pt;font-family:"Arial","sans-serif"'>Dr Raj<o:p></o:p></span></p></div><div><p class=MsoNormal><span lang=EN-US style='font-size:10.0pt;font-family:"Arial","sans-serif"'>Cellabs<o:p></o:p></span></p></div><div><p class=MsoNormal><span lang=EN-US style='font-size:10.0pt;font-family:"Arial","sans-serif"'>----- Original Message ----- <o:p></o:p></span></p><div><p class=MsoNormal><span lang=EN-US style='font-size:10.0pt;font-family:"Arial","sans-serif"'>From: "Hanafi, Hanafi CTR EG NAMRU3 " <<a href="mailto:hanafi.hanafi.ctr.eg@med.navy.mil">hanafi.hanafi.ctr.eg@med.navy.mil</a>><o:p></o:p></span></p></div><div><p class=MsoNormal><span lang=EN-US style='font-size:10.0pt;font-family:"Arial","sans-serif"'>To: "Raj" <<a href="mailto:raj@cellabs.com.au">raj@cellabs.com.au</a>>; <<a href="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</a>><o:p></o:p></span></p></div><div><p class=MsoNormal><span lang=EN-US style='font-size:10.0pt;font-family:"Arial","sans-serif"'>Sent: Tuesday, May 31, 2011 6:19 PM<o:p></o:p></span></p></div><div><p class=MsoNormal><span lang=EN-US style='font-size:10.0pt;font-family:"Arial","sans-serif"'>Subject: RE: [Leish-l] Leish-l Digest, Vol 41, Issue 9<o:p></o:p></span></p></div></div><div><p class=MsoNormal><span lang=EN-US style='font-size:10.0pt;font-family:"Arial","sans-serif"'><o:p> </o:p></span></p></div><p class=MsoNormal><span lang=EN-US style='font-size:10.0pt;font-family:"Arial","sans-serif"'>> Dear Dr Raj:<br>> <br>> I think you mean PKDL (post kala azar dermal leishmanisis) caused by L.<br>> donovani and it usually seen as a late complication of the disease. PKDL has<br>> been reported in India, Kenya and Sudan.<br>> <br>> Thank you <br>> R/<br>> Hanafi<br>> <br>> Hanafi A. Hanafi, Ph. D.<br>> Medical Research Scientist<br>> Vector Biology Research Program<br>> U.S. Naval Medical Research Unit No.3<br>> Cairo, Egypt<br>> Tel & Fax: ++2-02-2342-3090<br>> E-mail: <a href="mailto:hanafi.hanafi.ctr.eg@med.navy.mil">hanafi.hanafi.ctr.eg@med.navy.mil</a><br>> <a href="mailto:yahanafi@yahoo.com">yahanafi@yahoo.com</a><br>> </span><span lang=EN-US><o:p></o:p></span></p></div><p class=MsoNormal><span lang=EN-US><br><br><br><br></span><span lang=EN-US style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'><br>=======<br>Email scanned by PC Tools - No viruses or spyware found.<br>(Email Guard: 7.0.0.21, Virus/Spyware Database: 6.17630)<br><a href="http://www.pctools.com/?cclick=EmailFooterClean_51">http://www.pctools.com</a><br>=======</span><span lang=EN-US> <o:p></o:p></span></p><p class=MsoNormal><span lang=EN-US><br><br><br><br></span><span lang=EN-US style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'><br>=======<br>Email scanned by PC Tools - No viruses or spyware found.<br>(Email Guard: 7.0.0.21, Virus/Spyware Database: 6.17640)<br><a href="http://www.pctools.com/?cclick=EmailFooterClean_51">http://www.pctools.com</a><br>=======</span><span lang=EN-US> <o:p></o:p></span></p></blockquote><p class=MsoNormal><span lang=EN-US><br><br><br><br></span><span lang=EN-US style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'><br>=======<br>Email scanned by PC Tools - No viruses or spyware found.<br>(Email Guard: 7.0.0.21, Virus/Spyware Database: 6.17640)<br><a href="http://www.pctools.com/?cclick=EmailFooterClean_51">http://www.pctools.com</a><br>=======</span><span lang=EN-US> <o:p></o:p></span></p></div>
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