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<div class=Section1>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>Explanations for finding rare genotypes
are difficult without examining all variants exhaustively from a very large
sample size. It will require a significant effort to foster a significant collaboration
to achieve this. <o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>I have a dozen or so of lab cultured
Indian isolates. Should you be interested in having them, I would be happy to
send over ?<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>KP <o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'><o:p> </o:p></span></font></p>
<div>
<div class=MsoNormal align=center style='text-align:center'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'>
<hr size=2 width="100%" align=center tabindex=-1>
</span></font></div>
<p class=MsoNormal><b><font size=2 face=Tahoma><span style='font-size:10.0pt;
font-family:Tahoma;font-weight:bold'>From:</span></font></b><font size=2
face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma'> J.-C. Dujardin
[mailto:JCDujardin@itg.be] <br>
<b><span style='font-weight:bold'>Sent:</span></b> Wednesday, June 08, 2011
1:30 AM<br>
<b><span style='font-weight:bold'>To:</span></b> Raj; nandlals@hotmail.com;
Chang, Kwang-Poo; leish-l@lineu.icb.usp.br<br>
<b><span style='font-weight:bold'>Cc:</span></b> Matthew Berriman<br>
<b><span style='font-weight:bold'>Subject:</span></b> RE: [Leish-l] cutaneous
form of leish in Indian region</span></font><o:p></o:p></p>
</div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 color="#1f497d" face=Calibri><span
style='font-size:11.0pt;font-family:Calibri;color:#1F497D'>Dear all, I react
only now after seeing a lot of mails passing. We are currently undertaking a
major study on genetic diversity of L.donovani in the Indian sub-continent
(including full genome sequencing). While most of the strains analyzed so far
support the post-DDT bottleneck hypothesis (clonal expansion with low genetic
homogeneity), we start finding rare genotypes which are very different from the
major population. If you are interested, we might include your strains in our
analysis.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 color="#1f497d" face=Calibri><span
style='font-size:11.0pt;font-family:Calibri;color:#1F497D'>Best regards<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 color="#1f497d" face=Calibri><span
style='font-size:11.0pt;font-family:Calibri;color:#1F497D'>JC<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 color="#1f497d" face=Calibri><span
style='font-size:11.0pt;font-family:Calibri;color:#1F497D'><o:p> </o:p></span></font></p>
<div>
<p class=MsoNormal><font size=2 color="#1f497d" face=Calibri><span
style='font-size:11.0pt;font-family:Calibri;color:#1F497D'>Prof. Dr Jean-Claude
Dujardin<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 color="#1f497d" face=Calibri><span
style='font-size:11.0pt;font-family:Calibri;color:#1F497D'>Head of Molecular
Parasitology<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 color="#1f497d" face=Calibri><span lang=NL-BE
style='font-size:11.0pt;font-family:Calibri;color:#1F497D'>Instituut voor
Tropische Geneeskunde<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 color="#1f497d" face=Calibri><span lang=NL-BE
style='font-size:11.0pt;font-family:Calibri;color:#1F497D'>Nationalestraat, 155<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 color="#1f497d" face=Calibri><span lang=NL-BE
style='font-size:11.0pt;font-family:Calibri;color:#1F497D'>B-2000 Antwerpen<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 color="#1f497d" face=Calibri><span lang=NL-BE
style='font-size:11.0pt;font-family:Calibri;color:#1F497D'>Belgium<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 color="#1f497d" face=Calibri><span lang=NL-BE
style='font-size:11.0pt;font-family:Calibri;color:#1F497D'>Tel. 32.3.2476358 or
355<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 color="#1f497d" face=Calibri><span lang=NL-BE
style='font-size:11.0pt;font-family:Calibri;color:#1F497D'>Fax. 32.3.2476359<o:p></o:p></span></font></p>
</div>
<p class=MsoNormal><font size=2 color="#1f497d" face=Calibri><span lang=NL-BE
style='font-size:11.0pt;font-family:Calibri;color:#1F497D'><o:p> </o:p></span></font></p>
<div>
<div style='border:none;border-top:solid #B5C4DF 1.0pt;padding:3.0pt 0in 0in 0in'>
<p class=MsoNormal><b><font size=2 face=Tahoma><span style='font-size:10.0pt;
font-family:Tahoma;font-weight:bold'>From:</span></font></b><font size=2
face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma'>
leish-l-bounces@lineu.icb.usp.br [mailto:leish-l-bounces@lineu.icb.usp.br] <b><span
style='font-weight:bold'>On Behalf Of </span></b>Raj<br>
<b><span style='font-weight:bold'>Sent:</span></b> donderdag 2 juni 2011 8:16<br>
<b><span style='font-weight:bold'>To:</span></b> nandlals@hotmail.com; Chang,
Kwang-Poo; leish-l@lineu.icb.usp.br<br>
<b><span style='font-weight:bold'>Subject:</span></b> Re: [Leish-l] cutaneous
form of leish in Indian region<o:p></o:p></span></font></p>
</div>
</div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span lang=NL-BE
style='font-size:12.0pt'><o:p> </o:p></span></font></p>
<div>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Hi KP,</span></font><o:p></o:p></p>
</div>
<div>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Certainly I will do. Please suggest Dr NL Sharma to do so.</span></font><o:p></o:p></p>
</div>
<div>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Raj</span></font><o:p></o:p></p>
</div>
<blockquote style='border:none;border-left:solid black 1.5pt;padding:0in 0in 0in 4.0pt;
margin-left:3.75pt;margin-top:5.0pt;margin-right:0in;margin-bottom:5.0pt'>
<div>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>----- Original Message ----- <o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal style='background:#E4E4E4'><b><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial;font-weight:bold'>From:</span></font></b><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'> <a
href="mailto:KwangPoo.Chang@rosalindfranklin.edu"
title="KwangPoo.Chang@rosalindfranklin.edu">Chang, Kwang-Poo</a> <o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><b><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial;font-weight:bold'>To:</span></font></b><font size=2
face=Arial><span style='font-size:10.0pt;font-family:Arial'> <a
href="mailto:raj@cellabs.com.au" title="raj@cellabs.com.au">Raj</a> ; <a
href="mailto:hanafi.hanafi.ctr.eg@med.navy.mil"
title="hanafi.hanafi.ctr.eg@med.navy.mil">Hanafi, Hanafi CTR EG NAMRU3 </a>; <a
href="mailto:nandlals@hotmail.com" title="nandlals@hotmail.com">nandlals@hotmail.com</a>
; <a href="mailto:leish-l@lineu.icb.usp.br" title="leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</a>
<o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><b><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial;font-weight:bold'>Sent:</span></font></b><font size=2
face=Arial><span style='font-size:10.0pt;font-family:Arial'> Thursday, June 02,
2011 8:41 AM<o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><b><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial;font-weight:bold'>Subject:</span></font></b><font size=2
face=Arial><span style='font-size:10.0pt;font-family:Arial'> RE: cutaneous form
of leish in Indian region<o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><o:p> </o:p></span></font></p>
</div>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>Great if Dr. Sharma would be able to send
serum samples to you for evaluating your species-specific exo-antigens.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>KP<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'><o:p> </o:p></span></font></p>
<div>
<div class=MsoNormal align=center style='text-align:center'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'>
<hr size=2 width="100%" align=center>
</span></font></div>
<p class=MsoNormal><b><font size=2 face=Tahoma><span style='font-size:10.0pt;
font-family:Tahoma;font-weight:bold'>From:</span></font></b><font size=2
face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma'> Raj
[mailto:raj@cellabs.com.au] <br>
<b><span style='font-weight:bold'>Sent:</span></b> Tuesday, May 31, 2011 9:18
PM<br>
<b><span style='font-weight:bold'>To:</span></b> Hanafi, Hanafi CTR EG NAMRU3 ;
Chang, Kwang-Poo; <a href="mailto:nandlals@hotmail.com">nandlals@hotmail.com</a>;
<a href="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</a><br>
<b><span style='font-weight:bold'>Subject:</span></b> cutaneous form of leish
in Indian region</span></font><o:p></o:p></p>
</div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><o:p> </o:p></span></font></p>
<div>
<div>
<p class=MsoNormal><st1:Street w:st="on"><st1:address w:st="on"><font size=2
face=Arial><span style='font-size:10.0pt;font-family:Arial'>G'Day Dr</span></font></st1:address></st1:Street><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'> Hanafi,<o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>My enquiry was to know whether <em><i><font face=Arial><span
style='font-family:Arial'>L. donovani</span></font></i></em> in <st1:country-region
w:st="on"><st1:place w:st="on">India</st1:place></st1:country-region>
produces a cutaneous form of lesion or not (I am not referring
to PKDL form). There are many reports from SriLanka about the cutaneous
manifestations by <em><i><font face=Arial><span style='font-family:Arial'>L.
donovani</span></font></i></em>. The PKDL although is a
cutaneous response appears very late in the infection process
and this is ascribed to be a protective response against the
parasite. <o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Prof Chang mentioned <o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>"Analyses of several batches of basically clinical CL
samples from Dr. Sharma showed evidence of L infantum, but also L tropica as
well as a mixture of the two in one sample. This is the same picture we have
noted for samples from <st1:place w:st="on"><st1:City w:st="on">Hatay</st1:City>,
<st1:country-region w:st="on">Turkey</st1:country-region></st1:place>."
This is from <st1:place w:st="on">Sutlej</st1:place> region." It means
this cutaneous complex requires some investigation. <o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>I raise species-specific exo-antigens from <em><i><font
face=Arial><span style='font-family:Arial'>L. tropica</span></font></i></em>
and <em><i><font face=Arial><span style='font-family:Arial'>L infantum</span></font></i></em>
and I am prepared to test the serum /plasma samples for presence of
antibodies. If Dr NL Sharma is prepared to send me specimens as well as
clinical plasma or serum samples I would be interested to investigate the
antibody response in these situations. <o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Dr Raj<o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Cellabs<o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>----- Original Message ----- <o:p></o:p></span></font></p>
<div>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>From: "Hanafi, Hanafi CTR EG NAMRU3 " <<a
href="mailto:hanafi.hanafi.ctr.eg@med.navy.mil">hanafi.hanafi.ctr.eg@med.navy.mil</a>><o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>To: "Raj" <<a href="mailto:raj@cellabs.com.au">raj@cellabs.com.au</a>>;
<<a href="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</a>><o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Sent: Tuesday, May 31, 2011 6:19 PM<o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Subject: RE: [Leish-l] Leish-l Digest, Vol 41, Issue 9<o:p></o:p></span></font></p>
</div>
</div>
<div>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'><o:p> </o:p></span></font></p>
</div>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>> Dear Dr Raj:<br>
> <br>
> I think you mean PKDL (post kala azar dermal leishmanisis) caused by L.<br>
> donovani and it usually seen as a late complication of the disease. PKDL
has<br>
> been reported in <st1:country-region w:st="on">India</st1:country-region>,
<st1:country-region w:st="on">Kenya</st1:country-region> and <st1:country-region
w:st="on"><st1:place w:st="on">Sudan</st1:place></st1:country-region>.<br>
> <br>
> Thank you <br>
> R/<br>
> Hanafi<br>
> <br>
> Hanafi A. Hanafi, Ph. D.<br>
> Medical Research Scientist<br>
> Vector Biology Research Program<br>
> <st1:country-region w:st="on"><st1:place w:st="on">U.S.</st1:place></st1:country-region>
Naval Medical Research Unit No.3<br>
> <st1:place w:st="on"><st1:City w:st="on">Cairo</st1:City>, <st1:country-region
w:st="on">Egypt</st1:country-region></st1:place><br>
> Tel & Fax: ++2-02-2342-3090<br>
> E-mail: <a href="mailto:hanafi.hanafi.ctr.eg@med.navy.mil">hanafi.hanafi.ctr.eg@med.navy.mil</a><br>
> <a
href="mailto:yahanafi@yahoo.com">yahanafi@yahoo.com</a><br>
> </span></font><o:p></o:p></p>
</div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><br>
<br>
<br>
<br>
</span></font><font size=2 face=Tahoma><span style='font-size:10.0pt;
font-family:Tahoma'><br>
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=======</span></font> <o:p></o:p></p>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><br>
<br>
<br>
<br>
</span></font><font size=2 face=Tahoma><span style='font-size:10.0pt;
font-family:Tahoma'><br>
=======<br>
Email scanned by PC Tools - No viruses or spyware found.<br>
(Email Guard: 7.0.0.21, Virus/Spyware Database: 6.17640)<br>
<a href="http://www.pctools.com/?cclick=EmailFooterClean_51">http://www.pctools.com</a><br>
=======</span></font> <o:p></o:p></p>
</blockquote>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><br>
<br>
<br>
<br>
</span></font><font size=2 face=Tahoma><span style='font-size:10.0pt;
font-family:Tahoma'><br>
=======<br>
Email scanned by PC Tools - No viruses or spyware found.<br>
(Email Guard: 7.0.0.21, Virus/Spyware Database: 6.17640)<br>
<a href="http://www.pctools.com/?cclick=EmailFooterClean_51">http://www.pctools.com</a><br>
=======</span></font> <o:p></o:p></p>
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