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<DIV><FONT face=Calibri>Dear KP,</FONT></DIV>
<DIV><FONT face=Calibri>Yes you remember correctly that these strains are
different from common Indian Strain of L. donovani of Bihar. The three strains
examined at London School of tropical Medicine with each having different
homologies but they were also not L infantum. However, some other strains
examined at Rosalind franklin University had results which you
are mentioning. We had two dogs positive for rK 39 and one which could
follow, died after about 5 months.</FONT></DIV>
<DIV><FONT face=Calibri>Dr. Sunil Arora had also experience of handling these
strains but also could not maintain for long.</FONT></DIV>
<DIV><FONT face=Calibri>NL Sharma</FONT> </DIV>
<DIV style="FONT: 10pt Tahoma">
<DIV><BR></DIV>
<DIV style="BACKGROUND: #f5f5f5">
<DIV style="font-color: black"><B>From:</B> <A
title="mailto:KwangPoo.Chang@rosalindfranklin.edu CTRL + Click to follow link"
href="mailto:KwangPoo.Chang@rosalindfranklin.edu">Chang, Kwang-Poo</A> </DIV>
<DIV><B>Sent:</B> Thursday, June 02, 2011 11:40 PM</DIV>
<DIV><B>To:</B> <A title=nandlals@hotmail.com
href="mailto:nandlals@hotmail.com">Sharman</A> ; <A title=raj@cellabs.com.au
href="mailto:raj@cellabs.com.au">Raj</A> ; <A title=volf@cesnet.cz
href="mailto:volf@cesnet.cz">Petr Volf</A> ; <A
title="mailto:leish-l@lineu.icb.usp.br CTRL + Click to follow link"
href="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</A> ; <A
title="mailto:hgoto@usp.br CTRL + Click to follow link"
href="mailto:hgoto@usp.br">hgoto@usp.br</A> ; <A title=elfadil_abass@yahoo.com
href="mailto:elfadil_abass@yahoo.com">elfadil_abass@yahoo.com</A> ; <A
title="mailto:vishwamohan_katoch@yahoo.co.in CTRL + Click to follow link"
href="mailto:vishwamohan_katoch@yahoo.co.in">vishwamohan_katoch@yahoo.co.in</A>
</DIV>
<DIV><B>Subject:</B> RE: [Leish-l] inquiry</DIV></DIV></DIV>
<DIV><BR></DIV>
<DIV class=Section1>
<P class=MsoNormal><FONT color=navy size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; COLOR: navy; FONT-SIZE: 10pt">Dear Dr.
Sharma,<o:p></o:p></SPAN></FONT></P>
<P class=MsoNormal><FONT color=navy size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; COLOR: navy; FONT-SIZE: 10pt"><o:p> </o:p></SPAN></FONT></P>
<P class=MsoNormal><FONT color=navy size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; COLOR: navy; FONT-SIZE: 10pt">Is the L donovani in
the <st1:PlaceName w:st="on">Satluj</st1:PlaceName> <st1:PlaceType
w:st="on">River</st1:PlaceType> <st1:PlaceType w:st="on">Valley</st1:PlaceType>
different from the L donovani in <st1:place w:st="on">Bihar</st1:place> ? This
is an important point.<o:p></o:p></SPAN></FONT></P>
<P class=MsoNormal><FONT color=navy size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; COLOR: navy; FONT-SIZE: 10pt"><o:p> </o:p></SPAN></FONT></P>
<P class=MsoNormal><FONT color=navy size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; COLOR: navy; FONT-SIZE: 10pt">The genetic site we
examined did reveal identity of your samples with L infantum, but differs from L
donovani from Bihar and <st1:country-region w:st="on"><st1:place
w:st="on">Sudan</st1:place></st1:country-region>. Have you not found rK39+ dogs
? In one of your samples, we saw evidence of both L infantum and L
tropica. Whether this is a hybrid or a mixture of the two cannot be
certain.<o:p></o:p></SPAN></FONT></P>
<P class=MsoNormal><FONT color=navy size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; COLOR: navy; FONT-SIZE: 10pt"><o:p> </o:p></SPAN></FONT></P>
<P class=MsoNormal><FONT color=navy size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; COLOR: navy; FONT-SIZE: 10pt">It would be good if
Petr Volf could have an opportunity of looking into the sand flies there ?
<o:p></o:p></SPAN></FONT></P>
<P class=MsoNormal><FONT color=navy size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; COLOR: navy; FONT-SIZE: 10pt"><o:p> </o:p></SPAN></FONT></P>
<P class=MsoNormal><FONT color=navy size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; COLOR: navy; FONT-SIZE: 10pt">KP<o:p></o:p></SPAN></FONT></P>
<P class=MsoNormal><FONT color=navy size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; COLOR: navy; FONT-SIZE: 10pt"><o:p> </o:p></SPAN></FONT></P>
<DIV>
<DIV style="TEXT-ALIGN: center" class=MsoNormal align=center><FONT size=3
face="Times New Roman"><SPAN style="FONT-SIZE: 12pt">
<HR tabIndex=-1 align=center SIZE=2 width="100%">
</SPAN></FONT></DIV>
<P class=MsoNormal><B><FONT size=2 face=Tahoma><SPAN
style="FONT-FAMILY: Tahoma; FONT-SIZE: 10pt; FONT-WEIGHT: bold">From:</SPAN></FONT></B><FONT
size=2 face=Tahoma><SPAN style="FONT-FAMILY: Tahoma; FONT-SIZE: 10pt"> Sharman
[mailto:nandlals@hotmail.com] <BR><B><SPAN
style="FONT-WEIGHT: bold">Sent:</SPAN></B> Wednesday, June 01, 2011 11:40
AM<BR><B><SPAN style="FONT-WEIGHT: bold">To:</SPAN></B> Raj; Petr Volf; Chang,
Kwang-Poo; <A
href="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</A>; <A
href="mailto:hgoto@usp.br">hgoto@usp.br</A>; <A
href="mailto:elfadil_abass@yahoo.com">elfadil_abass@yahoo.com</A>; <A
href="mailto:vishwamohan_katoch@yahoo.co.in">vishwamohan_katoch@yahoo.co.in</A><BR><B><SPAN
style="FONT-WEIGHT: bold">Subject:</SPAN></B> Re: [Leish-l]
inquiry</SPAN></FONT><o:p></o:p></P></DIV>
<P class=MsoNormal><FONT size=3 face="Times New Roman"><SPAN
style="FONT-SIZE: 12pt"><o:p> </o:p></SPAN></FONT></P>
<DIV>
<P class=MsoNormal><FONT size=3 face=Calibri><SPAN
style="FONT-FAMILY: Calibri; FONT-SIZE: 12pt">Dear
all,</SPAN></FONT><o:p></o:p></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=3 face=Calibri><SPAN
style="FONT-FAMILY: Calibri; FONT-SIZE: 12pt">The discussion is getting very
interesting. To let you know our focus of CL in <st1:country-region
w:st="on"><st1:place w:st="on">India</st1:place></st1:country-region>, I.e.
Satluj Valley focus has got L donovani predominantly and L . tropica as well.
Two Labs conformed L donovani with approximately 4: 1 ratio to L. tropica.
Some more samples tested by Dr. Chang revealed L infantum and L
tropica. Results of First study were reported in AJTMH in 2005. L.infantum
results still unpublished. Another peculiar observation we observed in a child
patient was that after injecting SSG intralesionally white spots developed at
the injection sites. Whether this was toxicity of SSG or it is a counterpart of
PKDL as L. donovani is the cause is a question still to be answered. As I
mentioned earlier some of the patients' lesional exudate was also rK 39
positive. Well number of questions unanswered. Will try to find
answers.</SPAN></FONT><o:p></o:p></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=3 face=Calibri><SPAN
style="FONT-FAMILY: Calibri; FONT-SIZE: 12pt">NLS</SPAN></FONT> <o:p></o:p></P></DIV>
<DIV>
<DIV>
<P class=MsoNormal><FONT size=2 face=Tahoma><SPAN
style="FONT-FAMILY: Tahoma; FONT-SIZE: 10pt"><o:p> </o:p></SPAN></FONT></P></DIV>
<DIV>
<DIV style="font-color: black">
<P style="BACKGROUND: whitesmoke" class=MsoNormal><B><FONT size=2
face=Tahoma><SPAN
style="FONT-FAMILY: Tahoma; FONT-SIZE: 10pt; FONT-WEIGHT: bold">From:</SPAN></FONT></B><FONT
size=2 face=Tahoma><SPAN style="FONT-FAMILY: Tahoma; FONT-SIZE: 10pt"> <A
title=raj@cellabs.com.au href="mailto:raj@cellabs.com.au">Raj</A>
<o:p></o:p></SPAN></FONT></P></DIV>
<DIV>
<P style="BACKGROUND: whitesmoke" class=MsoNormal><B><FONT size=2
face=Tahoma><SPAN
style="FONT-FAMILY: Tahoma; FONT-SIZE: 10pt; FONT-WEIGHT: bold">Sent:</SPAN></FONT></B><FONT
size=2 face=Tahoma><SPAN style="FONT-FAMILY: Tahoma; FONT-SIZE: 10pt">
Wednesday, June 01, 2011 8:07 AM<o:p></o:p></SPAN></FONT></P></DIV>
<DIV>
<P style="BACKGROUND: whitesmoke" class=MsoNormal><B><FONT size=2
face=Tahoma><SPAN
style="FONT-FAMILY: Tahoma; FONT-SIZE: 10pt; FONT-WEIGHT: bold">To:</SPAN></FONT></B><FONT
size=2 face=Tahoma><SPAN style="FONT-FAMILY: Tahoma; FONT-SIZE: 10pt"> <A
title=volf@cesnet.cz href="mailto:volf@cesnet.cz">Petr Volf</A> ; <A
title=Kwang-Poo.Chang@rosalindfranklin.edu
href="mailto:Kwang-Poo.Chang@rosalindfranklin.edu">Chang, Kwang-Poo</A> ; <A
title=leish-l@lineu.icb.usp.br
href="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</A> ; <A
title=nandlals@hotmail.com
href="mailto:nandlals@hotmail.com">nandlals@hotmail.com</A> ; <A
title="mailto:hgoto@usp.br CTRL + Click to follow link"
href="mailto:hgoto@usp.br">hgoto@usp.br</A> ; <A
title="mailto:elfadil_abass@yahoo.com CTRL + Click to follow link"
href="mailto:elfadil_abass@yahoo.com">elfadil_abass@yahoo.com</A>
<o:p></o:p></SPAN></FONT></P></DIV>
<DIV>
<P style="BACKGROUND: whitesmoke" class=MsoNormal><B><FONT size=2
face=Tahoma><SPAN
style="FONT-FAMILY: Tahoma; FONT-SIZE: 10pt; FONT-WEIGHT: bold">Subject:</SPAN></FONT></B><FONT
size=2 face=Tahoma><SPAN style="FONT-FAMILY: Tahoma; FONT-SIZE: 10pt"> Re:
[Leish-l] inquiry<o:p></o:p></SPAN></FONT></P></DIV></DIV></DIV>
<DIV>
<P class=MsoNormal><FONT size=3 face="Times New Roman"><SPAN
style="FONT-SIZE: 12pt"><o:p> </o:p></SPAN></FONT></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt"> <st1:Street
w:st="on"><st1:address w:st="on">G'Day Dr</st1:address></st1:Street> Petr
Volf,</SPAN></FONT><o:p></o:p></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt">Thank you very much for your paper.
Excellent molecular typing of parasites. </SPAN></FONT><o:p></o:p></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt">As you see my correspondence below,
we have a antibody ELISA where we use exoantigens from individual <EM><I><FONT
face=Arial><SPAN style="FONT-FAMILY: Arial">L. infantum</SPAN></FONT></I></EM>
and <EM><I><FONT face=Arial><SPAN style="FONT-FAMILY: Arial">L.
tropica</SPAN></FONT></I></EM>. </SPAN></FONT><o:p></o:p></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt"> If you are interested I can
test plasma/serum samples for presence of antibody response. If so
please let me know.</SPAN></FONT><o:p></o:p></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=3 face="Times New Roman"><SPAN
style="FONT-SIZE: 12pt"> <o:p></o:p></SPAN></FONT></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt">Raj</SPAN></FONT><o:p></o:p></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt">Cellabs</SPAN></FONT><o:p></o:p></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=3 face="Times New Roman"><SPAN
style="FONT-SIZE: 12pt"> <o:p></o:p></SPAN></FONT></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=3 face="Times New Roman"><SPAN
style="FONT-SIZE: 12pt"> <o:p></o:p></SPAN></FONT></P></DIV>
<DIV>
<P class=MsoNormal><st1:Street w:st="on"><st1:address w:st="on"><FONT size=2
face=Arial><SPAN style="FONT-FAMILY: Arial; FONT-SIZE: 10pt">G'Day
Dr</SPAN></FONT></st1:address></st1:Street><FONT size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt">
Hanafi,</SPAN></FONT><o:p></o:p></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt">My enquiry was to know whether
<EM><I><FONT face=Arial><SPAN style="FONT-FAMILY: Arial">L.
donovani</SPAN></FONT></I></EM> in <st1:country-region w:st="on"><st1:place
w:st="on">India</st1:place></st1:country-region> produces a cutaneous form
of lesion or not (I am not referring to PKDL form). There are
many reports from SriLanka about the cutaneous manifestations
by <EM><I><FONT face=Arial><SPAN style="FONT-FAMILY: Arial">L.
donovani</SPAN></FONT></I></EM>. The PKDL although is a
cutaneous response appears very late in the infection process
and this is ascribed to be a protective response against the parasite.
</SPAN></FONT><o:p></o:p></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=3 face="Times New Roman"><SPAN
style="FONT-SIZE: 12pt"> <o:p></o:p></SPAN></FONT></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt">Prof Chang mentioned
</SPAN></FONT><o:p></o:p></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=3 face="Times New Roman"><SPAN
style="FONT-SIZE: 12pt">"Analyses of several batches of basically clinical CL
samples from Dr. Sharma showed evidence of L infantum, but also L tropica as
well as a mixture of the two in one sample. This is the same picture we have
noted for samples from <st1:place w:st="on"><st1:City
w:st="on">Hatay</st1:City>, <st1:country-region
w:st="on">Turkey</st1:country-region></st1:place>." This is from <st1:place
w:st="on">Sutlej</st1:place> region." It means this cutaneous complex
requires some investigation. <o:p></o:p></SPAN></FONT></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=3 face="Times New Roman"><SPAN
style="FONT-SIZE: 12pt"> <o:p></o:p></SPAN></FONT></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=3 face="Times New Roman"><SPAN
style="FONT-SIZE: 12pt">I raise species-specific exo-antigens from <EM><I><FONT
face="Times New Roman">L. tropica</FONT></I></EM> and <EM><I><FONT
face="Times New Roman">L infantum</FONT></I></EM> and I am prepared to test the
serum /plasma samples for presence of antibodies. If Dr NL Sharma is
prepared to send me specimens as well as clinical plasma or serum samples I
would be interested to investigate the antibody response in these situations.
<o:p></o:p></SPAN></FONT></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=3 face="Times New Roman"><SPAN
style="FONT-SIZE: 12pt"> <o:p></o:p></SPAN></FONT></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt">Dr
Raj</SPAN></FONT><o:p></o:p></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt">Cellabs</SPAN></FONT><o:p></o:p></P></DIV>
<BLOCKQUOTE
style="BORDER-BOTTOM: medium none; BORDER-LEFT: black 1pt solid; PADDING-BOTTOM: 0in; MARGIN: 5pt 0in 5pt 2.5pt; PADDING-LEFT: 3pt; PADDING-RIGHT: 0in; BORDER-TOP: medium none; BORDER-RIGHT: medium none; PADDING-TOP: 0in">
<DIV>
<P class=MsoNormal><FONT size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt">----- Original Message -----
<o:p></o:p></SPAN></FONT></P></DIV>
<DIV style="font-color: black">
<P style="BACKGROUND: #e4e4e4" class=MsoNormal><B><FONT size=2
face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt; FONT-WEIGHT: bold">From:</SPAN></FONT></B><FONT
size=2 face=Arial><SPAN style="FONT-FAMILY: Arial; FONT-SIZE: 10pt"> <A
title=volf@cesnet.cz href="mailto:volf@cesnet.cz">Petr Volf</A>
<o:p></o:p></SPAN></FONT></P></DIV>
<DIV>
<P class=MsoNormal><B><FONT size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt; FONT-WEIGHT: bold">To:</SPAN></FONT></B><FONT
size=2 face=Arial><SPAN style="FONT-FAMILY: Arial; FONT-SIZE: 10pt"> <A
title=raj@cellabs.com.au href="mailto:raj@cellabs.com.au">Raj</A>
<o:p></o:p></SPAN></FONT></P></DIV>
<DIV>
<P class=MsoNormal><B><FONT size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt; FONT-WEIGHT: bold">Sent:</SPAN></FONT></B><FONT
size=2 face=Arial><SPAN style="FONT-FAMILY: Arial; FONT-SIZE: 10pt"> Tuesday,
May 31, 2011 5:08 PM<o:p></o:p></SPAN></FONT></P></DIV>
<DIV>
<P class=MsoNormal><B><FONT size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt; FONT-WEIGHT: bold">Subject:</SPAN></FONT></B><FONT
size=2 face=Arial><SPAN style="FONT-FAMILY: Arial; FONT-SIZE: 10pt"> Fw:
[Leish-l] inquiry<o:p></o:p></SPAN></FONT></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=3 face="Times New Roman"><SPAN
style="FONT-SIZE: 12pt"><o:p> </o:p></SPAN></FONT></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt">Dear
Dr Raj,</SPAN></FONT><o:p></o:p></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt">I saw your question to Dr. Sharma.
I am forwarding my response to him as well the paper which might be
interesting also for you.</SPAN></FONT><o:p></o:p></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt">Best
wishes</SPAN></FONT><o:p></o:p></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt">Petr
Volf</SPAN></FONT><o:p></o:p></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=3 face="Times New Roman"><SPAN
style="FONT-SIZE: 12pt"> <o:p></o:p></SPAN></FONT></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt">----- Original Message -----
<o:p></o:p></SPAN></FONT></P>
<DIV style="font-color: black">
<P style="BACKGROUND: #e4e4e4" class=MsoNormal><B><FONT size=2
face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt; FONT-WEIGHT: bold">From:</SPAN></FONT></B><FONT
size=2 face=Arial><SPAN style="FONT-FAMILY: Arial; FONT-SIZE: 10pt"> <A
title=volf@cesnet.cz href="mailto:volf@cesnet.cz">Petr Volf</A>
<o:p></o:p></SPAN></FONT></P></DIV>
<DIV>
<P class=MsoNormal><B><FONT size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt; FONT-WEIGHT: bold">To:</SPAN></FONT></B><FONT
size=2 face=Arial><SPAN style="FONT-FAMILY: Arial; FONT-SIZE: 10pt"> <A
title=KwangPoo.Chang@rosalindfranklin.edu
href="mailto:KwangPoo.Chang@rosalindfranklin.edu">Chang, Kwang-Poo</A> ; <A
title=nandlals@hotmail.com href="mailto:nandlals@hotmail.com">Sharman</A> ; <A
title=hgoto@usp.br href="mailto:hgoto@usp.br">Hiro Goto</A> ; <A
title=elfadil_abass@yahoo.com href="mailto:elfadil_abass@yahoo.com">elfadil
abass</A> <o:p></o:p></SPAN></FONT></P></DIV>
<DIV>
<P class=MsoNormal><B><FONT size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt; FONT-WEIGHT: bold">Cc:</SPAN></FONT></B><FONT
size=2 face=Arial><SPAN style="FONT-FAMILY: Arial; FONT-SIZE: 10pt"> <A
title=leish-l@lineu.icb.usp.br
href="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</A>
<o:p></o:p></SPAN></FONT></P></DIV>
<DIV>
<P class=MsoNormal><B><FONT size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt; FONT-WEIGHT: bold">Sent:</SPAN></FONT></B><FONT
size=2 face=Arial><SPAN style="FONT-FAMILY: Arial; FONT-SIZE: 10pt"> Tuesday,
May 31, 2011 9:06 AM<o:p></o:p></SPAN></FONT></P></DIV>
<DIV>
<P class=MsoNormal><B><FONT size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt; FONT-WEIGHT: bold">Subject:</SPAN></FONT></B><FONT
size=2 face=Arial><SPAN style="FONT-FAMILY: Arial; FONT-SIZE: 10pt"> Re:
[Leish-l] inquiry<o:p></o:p></SPAN></FONT></P></DIV></DIV>
<DIV>
<P class=MsoNormal><FONT size=3 face="Times New Roman"><SPAN
style="FONT-SIZE: 12pt"><o:p> </o:p></SPAN></FONT></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt">Hi
K.P.,</SPAN></FONT><o:p></o:p></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt">in Cukurova region,
<st1:country-region w:st="on"><st1:place
w:st="on">Turkey</st1:place></st1:country-region>, cutaneous L. infantum (now
it seems that we are dealing with L.donovani/L. infantum hybrid) grew
very poorely if we isolated them from patients. Only 1 of 25 isolations was
succesfull. However, the same strain (confirmed by molecular methods) grew
repeatedly and very well if we isolated them from sandflies. It might be
useful for Dr. Sharma try to get isolates from sand flies. It is very
laborious but very useful, in Cukurova we got about dozen of isolates by this
method (all identical).</SPAN></FONT><o:p></o:p></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt">Patients are rK39 negative, see
attached paper.</SPAN></FONT><o:p></o:p></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt">Best
wishes</SPAN></FONT><o:p></o:p></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt">Petr</SPAN></FONT><o:p></o:p></P></DIV>
<BLOCKQUOTE
style="BORDER-BOTTOM: medium none; BORDER-LEFT: black 1pt solid; PADDING-BOTTOM: 0in; MARGIN: 5pt 0in 5pt 2.5pt; PADDING-LEFT: 3pt; PADDING-RIGHT: 0in; BORDER-TOP: medium none; BORDER-RIGHT: medium none; PADDING-TOP: 0in">
<DIV>
<P class=MsoNormal><FONT size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt">----- Original Message -----
<o:p></o:p></SPAN></FONT></P></DIV>
<DIV style="font-color: black">
<P style="BACKGROUND: #e4e4e4" class=MsoNormal><B><FONT size=2
face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt; FONT-WEIGHT: bold">From:</SPAN></FONT></B><FONT
size=2 face=Arial><SPAN style="FONT-FAMILY: Arial; FONT-SIZE: 10pt"> <A
title=KwangPoo.Chang@rosalindfranklin.edu
href="mailto:KwangPoo.Chang@rosalindfranklin.edu">Chang, Kwang-Poo</A>
<o:p></o:p></SPAN></FONT></P></DIV>
<DIV>
<P class=MsoNormal><B><FONT size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt; FONT-WEIGHT: bold">To:</SPAN></FONT></B><FONT
size=2 face=Arial><SPAN style="FONT-FAMILY: Arial; FONT-SIZE: 10pt"> <A
title=nandlals@hotmail.com href="mailto:nandlals@hotmail.com">Sharman</A> ;
<A title=hgoto@usp.br href="mailto:hgoto@usp.br">Hiro Goto</A> ; <A
title=elfadil_abass@yahoo.com href="mailto:elfadil_abass@yahoo.com">elfadil
abass</A> <o:p></o:p></SPAN></FONT></P></DIV>
<DIV>
<P class=MsoNormal><B><FONT size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt; FONT-WEIGHT: bold">Cc:</SPAN></FONT></B><FONT
size=2 face=Arial><SPAN style="FONT-FAMILY: Arial; FONT-SIZE: 10pt"> <A
title=leish-l@lineu.icb.usp.br
href="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</A>
<o:p></o:p></SPAN></FONT></P></DIV>
<DIV>
<P class=MsoNormal><B><FONT size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt; FONT-WEIGHT: bold">Sent:</SPAN></FONT></B><FONT
size=2 face=Arial><SPAN style="FONT-FAMILY: Arial; FONT-SIZE: 10pt"> Sunday,
May 22, 2011 1:39 AM<o:p></o:p></SPAN></FONT></P></DIV>
<DIV>
<P class=MsoNormal><B><FONT size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt; FONT-WEIGHT: bold">Subject:</SPAN></FONT></B><FONT
size=2 face=Arial><SPAN style="FONT-FAMILY: Arial; FONT-SIZE: 10pt"> Re:
[Leish-l] inquiry<o:p></o:p></SPAN></FONT></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=3 face="Times New Roman"><SPAN
style="FONT-SIZE: 12pt"><o:p> </o:p></SPAN></FONT></P></DIV>
<DIV id=idOWAReplyText41530>
<DIV>
<P class=MsoNormal><FONT color=black size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; COLOR: black; FONT-SIZE: 10pt">Dr. NL Sharma has
been working on an important CL endemic area along the <st1:PlaceName
w:st="on">Satluj</st1:PlaceName> <st1:PlaceType
w:st="on">River</st1:PlaceType> valley to the south of Himalaya in
<st1:country-region w:st="on"><st1:place
w:st="on">India</st1:place></st1:country-region> (Please correct me should I
be wrong for anything I said here and below). I had the good fortune of
visiting the site several years back courtesy of Dr. Sharma.
</SPAN></FONT><o:p></o:p></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=3 face="Times New Roman"><SPAN
style="FONT-SIZE: 12pt"> <o:p></o:p></SPAN></FONT></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt">I believe Dr. Sharma's finding
is important, since the parasites there are different from the familiar
Indian L donovani in <st1:place w:st="on">Bihar</st1:place>. The parasites
are refratory to in vitro cultivation. They do differentiate into
promastigotes and may grow a little, but can't really be
subcultured to establish passageable lines. This is very much reminescent of
L infantum in the Mediterranean area. I recall Dr. Sharma has also found
rK39+ dogs, if I remember correctly. If so, Satluj river valley
is endemic to the infantile CL.</SPAN></FONT><o:p></o:p></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=3 face="Times New Roman"><SPAN
style="FONT-SIZE: 12pt"> <o:p></o:p></SPAN></FONT></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt">Analyses of several batches of
basically clinical CL samples from Dr. Sharma showed evidence of L infantum,
but also L tropica as well as a mixture of the two in one sample. This
is the same picture we have noted for samples from <st1:place
w:st="on"><st1:City w:st="on">Hatay</st1:City>, <st1:country-region
w:st="on">Turkey</st1:country-region></st1:place>.</SPAN></FONT><o:p></o:p></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=3 face="Times New Roman"><SPAN
style="FONT-SIZE: 12pt"> <o:p></o:p></SPAN></FONT></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=3 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 12pt">These observations make me
wonder a lot about our current knowledge on the clinico-epidemiology based
on data collected previously by analyses of cultured promastigotes
from one or few 'representative samples'.
Nowaday, technology makes it very doable to work with biological
samples for Leish DNAs directly from sand flies, patients and reservoir
animals.</SPAN></FONT><o:p></o:p></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=3 face="Times New Roman"><SPAN
style="FONT-SIZE: 12pt"> <o:p></o:p></SPAN></FONT></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=2 face=Arial><SPAN
style="FONT-FAMILY: Arial; FONT-SIZE: 10pt">KP</SPAN></FONT><o:p></o:p></P></DIV>
<DIV>
<P class=MsoNormal><FONT size=3 face="Times New Roman"><SPAN
style="FONT-SIZE: 12pt"> <o:p></o:p></SPAN></FONT></P></DIV></DIV>
<DIV id=idSignature79278>
<DIV>
<P class=MsoNormal><FONT size=3 face="Times New Roman"><SPAN
style="FONT-SIZE: 12pt"> <o:p></o:p></SPAN></FONT></P></DIV>
<DIV>
<DIV style="TEXT-ALIGN: center" class=MsoNormal align=center><FONT size=3
face="Times New Roman"><SPAN style="FONT-SIZE: 12pt">
<HR tabIndex=-1 align=center SIZE=2 width="100%">
</SPAN></FONT></DIV>
<P style="MARGIN-BOTTOM: 12pt" class=MsoNormal><B><FONT size=2
face=Tahoma><SPAN
style="FONT-FAMILY: Tahoma; FONT-SIZE: 10pt; FONT-WEIGHT: bold">From:</SPAN></FONT></B><FONT
size=2 face=Tahoma><SPAN style="FONT-FAMILY: Tahoma; FONT-SIZE: 10pt">
leish-l-bounces@lineu.icb.usp.br on behalf of Sharman<BR><B><SPAN
style="FONT-WEIGHT: bold">Sent:</SPAN></B> Fri 5/20/2011 11:31
PM<BR><B><SPAN style="FONT-WEIGHT: bold">To:</SPAN></B> Hiro Goto; elfadil
abass<BR><B><SPAN style="FONT-WEIGHT: bold">Cc:</SPAN></B>
leish-l@lineu.icb.usp.br<BR><B><SPAN
style="FONT-WEIGHT: bold">Subject:</SPAN></B> Re: [Leish-l]
inquiry</SPAN></FONT><o:p></o:p></P></DIV></DIV>
<DIV>
<P><FONT size=2 face="Times New Roman"><SPAN style="FONT-SIZE: 10pt">Dear
all<BR>I agree with Hiro gito, We are working on a focus where the CL
is<BR>predominantly caused by L. donovani, and the rK 39 STRIPS GIVE
POSITIVE<BR>RESULTS WITH SERA as well as serous exudate from the lesion. The
results are<BR>dependent upon species.<BR>NL
Sharma<BR><BR>--------------------------------------------------<BR>From:
"Hiro Goto" <hgoto@usp.br><BR>Sent: Wednesday, May 18, 2011 12:09
AM<BR>To: "elfadil abass" <elfadil_abass@yahoo.com><BR>Cc:
<leish-l@lineu.icb.usp.br><BR>Subject: Re: [Leish-l]
inquiry<BR><BR>> Dear all,<BR>> In our oppinion, DAT and rK39 for
those samples are not indicated since<BR>> these tests are produced
for the diagnosis of visceral leishmaniasis.<BR>> In case of
tegumentary leishmaniasis, it is very appropriate the<BR>> observation of
J.J. Shaw appointing species specificity of antibody<BR>> response in
these cases. Titers of antibodies are in general low in these<BR>>
cases therefore depending on the species, it may result negative. We
have<BR>> published a review recently in Expert Rev. Anti Infect.
Ther. 8(4),<BR>> 419?433 (2010), Current diagnosis and treatment of
cutaneous and<BR>> mucocutaneous leishmaniasis, where we raise this
point.<BR>> Hiro Goto<BR>><BR>> Citando elfadil abass
<elfadil_abass@yahoo.com>:<BR>><BR>>> Dear all I would
recommend using DAT and rK39 strip test to measure<BR>>>
antibody<BR>>> responses and to evaluate the diagnostic efficiency for
both tests in<BR>>> such group<BR>>> of
patients.<BR>>><BR>>> Elfadil Abass<BR>>>
________________________________________________________________________________<BR>>>
<st1:place w:st="on"><st1:PlaceType w:st="on">Institute</st1:PlaceType> of
<st1:PlaceName w:st="on">Medical</st1:PlaceName></st1:place> Microbiology
and Hospital Hygiene<BR>>> <st1:PlaceName
w:st="on">Philipps</st1:PlaceName> <st1:PlaceName
w:st="on">University</st1:PlaceName> <st1:City w:st="on"><st1:place
w:st="on">Marburg</st1:place></st1:City><BR>>> BMFZ / Hans-Meerwein
Straße 2<BR>>> D-35033 <st1:place w:st="on"><st1:City
w:st="on">Marburg</st1:City>, <st1:country-region
w:st="on">Germany</st1:country-region></st1:place><BR>>>
________________________________________________________________________________<BR>>><BR>>><BR>>><BR>>><BR>>>
________________________________<BR>>> From: Nuha Nuwayri-Salti
<racha@aub.edu.lb><BR>>> To: saad saad
<saad1426@gmail.com>; "leish-l@lineu.icb.usp.br"<BR>>>
<leish-l@lineu.icb.usp.br><BR>>> Sent: Fri, May 13, 2011 9:39:33
AM<BR>>> Subject: Re: [Leish-l] inquiry<BR>>><BR>>> Dear
Saad first precaution to take is to separate your samples into<BR>>>
several<BR>>> portions each (at least 5 each being no more than a few
hundred(200-400)<BR>>> microliters). This is a necessary precaution to
avoid freezing and<BR>>> thawing<BR>>> several times the same
sample which will be the case should you do<BR>>>
different<BR>>> studies at different times which is
unavoidable.<BR>>><BR>>> What you can do is correlate the
type(ulcerated, abscess, furuncle<BR>>> etc.. ) the<BR>>>
locale, the number and age of lesions with the levels of antibody in
the<BR>>> sera of<BR>>> these patgients and also monitor cell
mediated immunity with leishmanin<BR>>> skin<BR>>>
test!<BR>>> I have just published (in print) an article about having
circulating<BR>>> parasites<BR>>> in some of these patients with
apparently pure cutaneous disease. It is<BR>>> the<BR>>> first
paper that revealed this fact. It would be interesting to
confirm<BR>>> or<BR>>> de-confirm this fact repeating what we
did.<BR>>> Best wishes<BR>>><BR>>> Nuha Nuwayri-Salti
MD<BR>>><BR>>><BR>>><BR>>> AOA<BR>>> Medical
Honor Society<BR>>><BR>>><BR>>><BR>>><BR>>>
-----Original Message-----<BR>>> From:
leish-l-bounces@lineu.icb.usp.br<BR>>> [<A
href="mailto:leish-l-bounces@lineu.icb.usp.br">mailto:leish-l-bounces@lineu.icb.usp.br</A>]<BR>>>
On Behalf Of saad saad<BR>>> Sent: Friday, April 29, 2011 11:23
PM<BR>>> To: leish-l@lineu.icb.usp.br<BR>>> Subject: [Leish-l]
inquiry<BR>>><BR>>> Dear all<BR>>> Hi. i am Saad from
<st1:country-region w:st="on"><st1:place w:st="on">Saudi
Arabia</st1:place></st1:country-region>. i have 60 sera from positive case
of<BR>>> CL from south west of the country. I would like to have
your<BR>>> recommendations to start a good research line in CL using
these sera.<BR>>> Thanks in advance for your help<BR>>>
_______________________________________________<BR>>> Leish-l mailing
list<BR>>> Leish-l@lineu.icb.usp.br<BR>>> <A
href="http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l">http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l</A><BR>>>
_______________________________________________<BR>>> Leish-l mailing
list<BR>>> Leish-l@lineu.icb.usp.br<BR>>> <A
href="http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l">http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l</A><BR>>><BR>><BR>><BR>><BR>>
Profa. Dra. Hiro Goto<BR>> Laboratório de Soroepidemiologia e
Imunobiologia<BR>> Instituto de Medicina Tropical de <st1:City
w:st="on"><st1:place w:st="on">São Paulo</st1:place></st1:City>, USP<BR>>
Av. Dr. Enéas de Carvalho Aguiar, 470, prédio II, quarto andar<BR>>
05403-000 - <st1:City w:st="on"><st1:place w:st="on">São
Paulo</st1:place></st1:City>, SP<BR>> Tel. +55-11-3061 7023, 3061 7056 ou
3061 7027<BR>> Fax. +55-11-3061-8270<BR>><BR>>
_______________________________________________<BR>> Leish-l mailing
list<BR>> Leish-l@lineu.icb.usp.br<BR>> <A
href="http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l">http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l</A><BR>><BR>_______________________________________________<BR>Leish-l
mailing list<BR>Leish-l@lineu.icb.usp.br<BR><A
href="http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l">http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l</A></SPAN></FONT><o:p></o:p></P></DIV>
<DIV style="TEXT-ALIGN: center" class=MsoNormal align=center><FONT size=3
face="Times New Roman"><SPAN style="FONT-SIZE: 12pt">
<HR align=center SIZE=2 width="100%">
</SPAN></FONT></DIV>
<P class=MsoNormal><FONT size=3 face="Times New Roman"><SPAN
style="FONT-SIZE: 12pt">_______________________________________________<BR>Leish-l
mailing
list<BR>Leish-l@lineu.icb.usp.br<BR>http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l<o:p></o:p></SPAN></FONT></P></BLOCKQUOTE>
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