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<DIV><FONT face=Calibri>Dear all,</FONT></DIV>
<DIV><FONT face=Calibri>The discussion is getting very interesting. To let you
know our focus of CL in India, I.e. Satluj Valley focus has got L donovani
predominantly and L . tropica as well. Two Labs conformed L donovani with
approximately 4: 1 ratio to L. tropica. Some more
samples tested by Dr. Chang revealed L infantum and L tropica.
Results of First study were reported in AJTMH in 2005. L.infantum results still
unpublished. Another peculiar observation we observed in a child patient was
that after injecting SSG intralesionally white spots developed at the injection
sites. Whether this was toxicity of SSG or it is a counterpart of PKDL as L.
donovani is the cause is a question still to be answered. As I mentioned earlier
some of the patients' lesional exudate was also rK 39 positive. Well number of
questions unanswered. Will try to find answers.</FONT></DIV>
<DIV><FONT face=Calibri>NLS</FONT> </DIV>
<DIV style="FONT: 10pt Tahoma">
<DIV><FONT size=3 face=Calibri></FONT><BR></DIV>
<DIV style="BACKGROUND: #f5f5f5">
<DIV style="font-color: black"><B>From:</B> <A title=raj@cellabs.com.au
href="mailto:raj@cellabs.com.au">Raj</A> </DIV>
<DIV><B>Sent:</B> Wednesday, June 01, 2011 8:07 AM</DIV>
<DIV><B>To:</B> <A title=volf@cesnet.cz href="mailto:volf@cesnet.cz">Petr
Volf</A> ; <A title=Kwang-Poo.Chang@rosalindfranklin.edu
href="mailto:Kwang-Poo.Chang@rosalindfranklin.edu">Chang, Kwang-Poo</A> ; <A
title=leish-l@lineu.icb.usp.br
href="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</A> ; <A
title=nandlals@hotmail.com
href="mailto:nandlals@hotmail.com">nandlals@hotmail.com</A> ; <A
title="mailto:hgoto@usp.br CTRL + Click to follow link"
href="mailto:hgoto@usp.br">hgoto@usp.br</A> ; <A
title="mailto:elfadil_abass@yahoo.com CTRL + Click to follow link"
href="mailto:elfadil_abass@yahoo.com">elfadil_abass@yahoo.com</A> </DIV>
<DIV><B>Subject:</B> Re: [Leish-l] inquiry</DIV></DIV></DIV>
<DIV><BR></DIV>
<DIV><FONT size=2 face=Arial> G'Day Dr Petr Volf,</FONT></DIV>
<DIV><FONT size=2 face=Arial>Thank you very much for your paper. Excellent
molecular typing of parasites. </FONT></DIV>
<DIV><FONT size=2 face=Arial>As you see my correspondence below, we have a
antibody ELISA where we use exoantigens from individual <EM>L. infantum</EM> and
<EM>L. tropica</EM>. </FONT></DIV>
<DIV><FONT size=2 face=Arial> If you are interested I can test plasma/serum
samples for presence of antibody response. If so please let me
know.</FONT></DIV>
<DIV><FONT size=2 face=Arial></FONT> </DIV>
<DIV><FONT size=2 face=Arial>Raj</FONT></DIV>
<DIV><FONT size=2 face=Arial>Cellabs</FONT></DIV>
<DIV><FONT size=2 face=Arial></FONT> </DIV>
<DIV><FONT size=2 face=Arial></FONT> </DIV>
<DIV><FONT size=2 face=Arial>G'Day Dr Hanafi,</FONT></DIV>
<DIV><FONT size=2 face=Arial>My enquiry was to know whether <EM>L. donovani</EM>
in India produces a cutaneous form of lesion or not (I am
not referring to PKDL form). There are many reports from SriLanka
about the cutaneous manifestations by <EM>L. donovani</EM>. The
PKDL although is a cutaneous response appears very late in
the infection process and this is ascribed to be a protective
response against the parasite. </FONT></DIV>
<DIV><FONT size=2 face=Arial></FONT> </DIV>
<DIV><FONT size=2 face=Arial>Prof Chang mentioned </FONT></DIV>
<DIV>"Analyses of several batches of basically clinical CL samples from Dr.
Sharma showed evidence of L infantum, but also L tropica as well as a mixture of
the two in one sample. This is the same picture we have noted for samples from
Hatay, Turkey." This is from Sutlej region." It means this cutaneous
complex requires some investigation. </DIV>
<DIV> </DIV>
<DIV>I raise species-specific exo-antigens from <EM>L. tropica</EM> and <EM>L
infantum</EM> and I am prepared to test the serum /plasma samples for presence
of antibodies. If Dr NL Sharma is prepared to send me specimens as well as
clinical plasma or serum samples I would be interested to investigate the
antibody response in these situations. </DIV>
<DIV> </DIV>
<DIV><FONT size=2 face=Arial>Dr Raj</FONT></DIV>
<DIV><FONT size=2 face=Arial>Cellabs</FONT></DIV>
<BLOCKQUOTE
style="BORDER-LEFT: #000000 2px solid; PADDING-LEFT: 5px; PADDING-RIGHT: 0px; MARGIN-LEFT: 5px; MARGIN-RIGHT: 0px">
<DIV style="FONT: 10pt arial">----- Original Message ----- </DIV>
<DIV
style="FONT: 10pt arial; BACKGROUND: #e4e4e4; font-color: black"><B>From:</B>
<A title=volf@cesnet.cz href="mailto:volf@cesnet.cz">Petr Volf</A> </DIV>
<DIV style="FONT: 10pt arial"><B>To:</B> <A title=raj@cellabs.com.au
href="mailto:raj@cellabs.com.au">Raj</A> </DIV>
<DIV style="FONT: 10pt arial"><B>Sent:</B> Tuesday, May 31, 2011 5:08 PM</DIV>
<DIV style="FONT: 10pt arial"><B>Subject:</B> Fw: [Leish-l] inquiry</DIV>
<DIV><BR></DIV>
<DIV><FONT size=2 face=Arial>Dear Dr Raj,</FONT></DIV>
<DIV><FONT size=2 face=Arial>I saw your question to Dr. Sharma. I am
forwarding my response to him as well the paper which might be
interesting also for you.</FONT></DIV>
<DIV><FONT size=2 face=Arial>Best wishes</FONT></DIV>
<DIV><FONT size=2 face=Arial>Petr Volf</FONT></DIV>
<DIV> </DIV>
<DIV style="FONT: 10pt arial">----- Original Message -----
<DIV style="BACKGROUND: #e4e4e4; font-color: black"><B>From:</B> <A
title=volf@cesnet.cz href="mailto:volf@cesnet.cz">Petr Volf</A> </DIV>
<DIV><B>To:</B> <A title=KwangPoo.Chang@rosalindfranklin.edu
href="mailto:KwangPoo.Chang@rosalindfranklin.edu">Chang, Kwang-Poo</A> ; <A
title=nandlals@hotmail.com href="mailto:nandlals@hotmail.com">Sharman</A> ; <A
title=hgoto@usp.br href="mailto:hgoto@usp.br">Hiro Goto</A> ; <A
title=elfadil_abass@yahoo.com href="mailto:elfadil_abass@yahoo.com">elfadil
abass</A> </DIV>
<DIV><B>Cc:</B> <A title=leish-l@lineu.icb.usp.br
href="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</A> </DIV>
<DIV><B>Sent:</B> Tuesday, May 31, 2011 9:06 AM</DIV>
<DIV><B>Subject:</B> Re: [Leish-l] inquiry</DIV></DIV>
<DIV><BR></DIV>
<DIV><FONT size=2 face=Arial>Hi K.P.,</FONT></DIV>
<DIV><FONT size=2 face=Arial>in Cukurova region, Turkey, cutaneous L. infantum
(now it seems that we are dealing with L.donovani/L. infantum hybrid)
grew very poorely if we isolated them from patients. Only 1 of 25 isolations
was succesfull. However, the same strain (confirmed by molecular methods) grew
repeatedly and very well if we isolated them from sandflies. It might be
useful for Dr. Sharma try to get isolates from sand flies. It is very
laborious but very useful, in Cukurova we got about dozen of isolates by this
method (all identical).</FONT></DIV>
<DIV><FONT size=2 face=Arial>Patients are rK39 negative, see attached
paper.</FONT></DIV>
<DIV><FONT size=2 face=Arial>Best wishes</FONT></DIV>
<DIV><FONT size=2 face=Arial>Petr</FONT></DIV>
<BLOCKQUOTE
style="BORDER-LEFT: #000000 2px solid; PADDING-LEFT: 5px; PADDING-RIGHT: 0px; MARGIN-LEFT: 5px; MARGIN-RIGHT: 0px">
<DIV style="FONT: 10pt arial">----- Original Message ----- </DIV>
<DIV
style="FONT: 10pt arial; BACKGROUND: #e4e4e4; font-color: black"><B>From:</B>
<A title=KwangPoo.Chang@rosalindfranklin.edu
href="mailto:KwangPoo.Chang@rosalindfranklin.edu">Chang, Kwang-Poo</A>
</DIV>
<DIV style="FONT: 10pt arial"><B>To:</B> <A title=nandlals@hotmail.com
href="mailto:nandlals@hotmail.com">Sharman</A> ; <A title=hgoto@usp.br
href="mailto:hgoto@usp.br">Hiro Goto</A> ; <A title=elfadil_abass@yahoo.com
href="mailto:elfadil_abass@yahoo.com">elfadil abass</A> </DIV>
<DIV style="FONT: 10pt arial"><B>Cc:</B> <A title=leish-l@lineu.icb.usp.br
href="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</A> </DIV>
<DIV style="FONT: 10pt arial"><B>Sent:</B> Sunday, May 22, 2011 1:39
AM</DIV>
<DIV style="FONT: 10pt arial"><B>Subject:</B> Re: [Leish-l] inquiry</DIV>
<DIV><BR></DIV>
<DIV dir=ltr id=idOWAReplyText41530>
<DIV dir=ltr><FONT color=#000000 size=2 face=Arial>Dr. NL Sharma has been
working on an important CL endemic area along the Satluj River
valley to the south of Himalaya in India (Please correct me should I be
wrong for anything I said here and below). I had the good fortune of
visiting the site several years back courtesy of Dr. Sharma. </FONT></DIV>
<DIV dir=ltr><FONT size=2 face=Arial></FONT> </DIV>
<DIV dir=ltr><FONT size=2 face=Arial>I believe Dr. Sharma's finding is
important, since the parasites there are different from the familiar Indian
L donovani in Bihar. The parasites are refratory to in vitro cultivation.
They do differentiate into promastigotes and may grow a little, but
can't really be subcultured to establish passageable lines. This is
very much reminescent of L infantum in the Mediterranean area. I recall Dr.
Sharma has also found rK39+ dogs, if I remember correctly. If
so, Satluj river valley is endemic to the infantile
CL.</FONT></DIV>
<DIV dir=ltr><FONT size=2 face=Arial></FONT> </DIV>
<DIV dir=ltr><FONT size=2 face=Arial>Analyses of several batches of
basically clinical CL samples from Dr. Sharma showed evidence of L infantum,
but also L tropica as well as a mixture of the two in one sample. This
is the same picture we have noted for samples from Hatay,
Turkey.</FONT></DIV>
<DIV dir=ltr><FONT face=Arial><FONT size=2><FONT
size=+0></FONT></FONT></FONT> </DIV>
<DIV dir=ltr><FONT face=Arial><FONT size=2><FONT size=+0>These observations
make me wonder a lot about our current knowledge on the clinico-epidemiology
based on data collected previously by analyses of cultured promastigotes
from one or few 'representative samples'.
Nowaday, technology makes it very doable to work with biological
samples for Leish DNAs directly from sand flies, patients and reservoir
animals.</FONT></FONT></FONT></DIV>
<DIV dir=ltr><FONT size=2 face=Arial></FONT> </DIV>
<DIV dir=ltr><FONT size=2 face=Arial>KP</FONT></DIV>
<DIV dir=ltr><FONT color=#000000 size=2 face=Arial></FONT> </DIV></DIV>
<DIV dir=ltr id=idSignature79278>
<DIV> </DIV>
<DIV>
<HR tabIndex=-1>
<FONT size=2 face=Tahoma><B>From:</B> leish-l-bounces@lineu.icb.usp.br on
behalf of Sharman<BR><B>Sent:</B> Fri 5/20/2011 11:31 PM<BR><B>To:</B> Hiro
Goto; elfadil abass<BR><B>Cc:</B>
leish-l@lineu.icb.usp.br<BR><B>Subject:</B> Re: [Leish-l]
inquiry<BR></FONT><BR></DIV></DIV>
<DIV>
<P><FONT size=2>Dear all<BR>I agree with Hiro gito, We are working on a
focus where the CL is<BR>predominantly caused by L. donovani, and the rK 39
STRIPS GIVE POSITIVE<BR>RESULTS WITH SERA as well as serous exudate from the
lesion. The results are<BR>dependent upon species.<BR>NL
Sharma<BR><BR>--------------------------------------------------<BR>From:
"Hiro Goto" <hgoto@usp.br><BR>Sent: Wednesday, May 18, 2011 12:09
AM<BR>To: "elfadil abass" <elfadil_abass@yahoo.com><BR>Cc:
<leish-l@lineu.icb.usp.br><BR>Subject: Re: [Leish-l]
inquiry<BR><BR>> Dear all,<BR>> In our oppinion, DAT and rK39 for
those samples are not indicated since<BR>> these tests are produced
for the diagnosis of visceral leishmaniasis.<BR>> In case of
tegumentary leishmaniasis, it is very appropriate the<BR>> observation of
J.J. Shaw appointing species specificity of antibody<BR>> response in
these cases. Titers of antibodies are in general low in these<BR>>
cases therefore depending on the species, it may result negative. We
have<BR>> published a review recently in Expert Rev. Anti Infect.
Ther. 8(4),<BR>> 419?433 (2010), Current diagnosis and treatment of
cutaneous and<BR>> mucocutaneous leishmaniasis, where we raise this
point.<BR>> Hiro Goto<BR>><BR>> Citando elfadil abass
<elfadil_abass@yahoo.com>:<BR>><BR>>> Dear all I would
recommend using DAT and rK39 strip test to measure<BR>>>
antibody<BR>>> responses and to evaluate the diagnostic efficiency for
both tests in<BR>>> such group<BR>>> of
patients.<BR>>><BR>>> Elfadil Abass<BR>>>
________________________________________________________________________________<BR>>>
Institute of Medical Microbiology and Hospital Hygiene<BR>>> Philipps
University Marburg<BR>>> BMFZ / Hans-Meerwein Straße 2<BR>>>
D-35033 Marburg, Germany<BR>>>
________________________________________________________________________________<BR>>><BR>>><BR>>><BR>>><BR>>>
________________________________<BR>>> From: Nuha Nuwayri-Salti
<racha@aub.edu.lb><BR>>> To: saad saad
<saad1426@gmail.com>; "leish-l@lineu.icb.usp.br"<BR>>>
<leish-l@lineu.icb.usp.br><BR>>> Sent: Fri, May 13, 2011 9:39:33
AM<BR>>> Subject: Re: [Leish-l] inquiry<BR>>><BR>>> Dear
Saad first precaution to take is to separate your samples into<BR>>>
several<BR>>> portions each (at least 5 each being no more than a few
hundred(200-400)<BR>>> microliters). This is a necessary precaution to
avoid freezing and<BR>>> thawing<BR>>> several times the same
sample which will be the case should you do<BR>>>
different<BR>>> studies at different times which is
unavoidable.<BR>>><BR>>> What you can do is correlate the
type(ulcerated, abscess, furuncle<BR>>> etc.. ) the<BR>>>
locale, the number and age of lesions with the levels of antibody in
the<BR>>> sera of<BR>>> these patgients and also monitor cell
mediated immunity with leishmanin<BR>>> skin<BR>>>
test!<BR>>> I have just published (in print) an article about having
circulating<BR>>> parasites<BR>>> in some of these patients with
apparently pure cutaneous disease. It is<BR>>> the<BR>>> first
paper that revealed this fact. It would be interesting to
confirm<BR>>> or<BR>>> de-confirm this fact repeating what we
did.<BR>>> Best wishes<BR>>><BR>>> Nuha Nuwayri-Salti
MD<BR>>><BR>>><BR>>><BR>>> AOA<BR>>> Medical
Honor Society<BR>>><BR>>><BR>>><BR>>><BR>>>
-----Original Message-----<BR>>> From:
leish-l-bounces@lineu.icb.usp.br<BR>>> [<A
href="mailto:leish-l-bounces@lineu.icb.usp.br">mailto:leish-l-bounces@lineu.icb.usp.br</A>]<BR>>>
On Behalf Of saad saad<BR>>> Sent: Friday, April 29, 2011 11:23
PM<BR>>> To: leish-l@lineu.icb.usp.br<BR>>> Subject: [Leish-l]
inquiry<BR>>><BR>>> Dear all<BR>>> Hi. i am Saad from
Saudi Arabia. i have 60 sera from positive case of<BR>>> CL from south
west of the country. I would like to have your<BR>>> recommendations
to start a good research line in CL using these sera.<BR>>> Thanks in
advance for your help<BR>>>
_______________________________________________<BR>>> Leish-l mailing
list<BR>>> Leish-l@lineu.icb.usp.br<BR>>> <A
href="http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l">http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l</A><BR>>>
_______________________________________________<BR>>> Leish-l mailing
list<BR>>> Leish-l@lineu.icb.usp.br<BR>>> <A
href="http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l">http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l</A><BR>>><BR>><BR>><BR>><BR>>
Profa. Dra. Hiro Goto<BR>> Laboratório de Soroepidemiologia e
Imunobiologia<BR>> Instituto de Medicina Tropical de São Paulo,
USP<BR>> Av. Dr. Enéas de Carvalho Aguiar, 470, prédio II, quarto
andar<BR>> 05403-000 - São Paulo, SP<BR>> Tel. +55-11-3061 7023, 3061
7056 ou 3061 7027<BR>> Fax. +55-11-3061-8270<BR>><BR>>
_______________________________________________<BR>> Leish-l mailing
list<BR>> Leish-l@lineu.icb.usp.br<BR>> <A
href="http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l">http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l</A><BR>><BR>_______________________________________________<BR>Leish-l
mailing list<BR>Leish-l@lineu.icb.usp.br<BR><A
href="http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l">http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l</A><BR></FONT></P></DIV>
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