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<DIV dir=ltr><FONT color=#000000 size=2 face=Arial>Dr. Sharma's cultures produce promastigotes, which cannot be passaged to build up the population, if I remember correctly. </FONT></DIV>
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<DIV dir=ltr><FONT size=2 face=Arial>Petr will be great to help Dr. Sharma out with the sandfly studies.</FONT></DIV>
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<DIV dir=ltr><FONT size=2 face=Arial>KP</FONT></DIV></DIV>
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<FONT size=2 face=Tahoma><B>From:</B> Sharman [mailto:nandlals@hotmail.com]<BR><B>Sent:</B> Tue 5/31/2011 10:13 AM<BR><B>To:</B> Petr Volf; Chang, Kwang-Poo; Hiro Goto; elfadil abass; vishwamohan_katoch@yahoo.co.in; lalit Kant ICMR<BR><B>Cc:</B> leish-l@lineu.icb.usp.br<BR><B>Subject:</B> Re: [Leish-l] inquiry<BR></FONT><BR></DIV>
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<DIV><FONT face=Calibri>Hi Petr,</FONT></DIV>
<DIV><FONT face=Calibri>Thanks for enclosing the pdf. In fact the strain which we are dealing with is not very difficult to grow but difficult to maintain, It is rK 39 positive and as I mentioned in the conversation below that even the serous exudate from the ulcer is also positive for rK 39 dipstick. In between because of my relocation to a different institute I was little away from this work but I intend to resume this work in couple of months. Although in past we tried to isolate the parasite from sand flies but did not succeed. We will surely attempt again with the techniques described by you and others. Contamination is the problem.</FONT></DIV>
<DIV><FONT face=Calibri>This particular focus seems to be a complex one.</FONT></DIV>
<DIV><FONT face=Calibri>NL Sharma</FONT> </DIV>
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<DIV><B>From:</B> <A title=volf@cesnet.cz href="mailto:volf@cesnet.cz">Petr Volf</A> </DIV>
<DIV><B>Sent:</B> Tuesday, May 31, 2011 12:36 PM</DIV>
<DIV><B>To:</B> <A title=KwangPoo.Chang@rosalindfranklin.edu href="mailto:KwangPoo.Chang@rosalindfranklin.edu">Chang, Kwang-Poo</A> ; <A title="mailto:nandlals@hotmail.com
CTRL + Click to follow link" href="mailto:nandlals@hotmail.com">Sharman</A> ; <A title="mailto:hgoto@usp.br
CTRL + Click to follow link" href="mailto:hgoto@usp.br">Hiro Goto</A> ; <A title="mailto:elfadil_abass@yahoo.com
CTRL + Click to follow link" href="mailto:elfadil_abass@yahoo.com">elfadil abass</A> </DIV>
<DIV><B>Cc:</B> <A title=leish-l@lineu.icb.usp.br href="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</A> </DIV>
<DIV><B>Subject:</B> Re: [Leish-l] inquiry</DIV></DIV></DIV>
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<DIV><FONT size=2 face=Arial>Hi K.P.,</FONT></DIV>
<DIV><FONT size=2 face=Arial>in Cukurova region, Turkey, cutaneous L. infantum (now it seems that we are dealing with L.donovani/L. infantum hybrid) grew very poorely if we isolated them from patients. Only 1 of 25 isolations was succesfull. However, the same strain (confirmed by molecular methods) grew repeatedly and very well if we isolated them from sandflies. It might be useful for Dr. Sharma try to get isolates from sand flies. It is very laborious but very useful, in Cukurova we got about dozen of isolates by this method (all identical).</FONT></DIV>
<DIV><FONT size=2 face=Arial>Patients are rK39 negative, see attached paper.</FONT></DIV>
<DIV><FONT size=2 face=Arial>Best wishes</FONT></DIV>
<DIV><FONT size=2 face=Arial>Petr</FONT></DIV>
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<DIV style="FONT: 10pt arial">----- Original Message ----- </DIV>
<DIV style="FONT: 10pt arial; BACKGROUND: #e4e4e4"><B>From:</B> <A title=KwangPoo.Chang@rosalindfranklin.edu href="mailto:KwangPoo.Chang@rosalindfranklin.edu">Chang, Kwang-Poo</A> </DIV>
<DIV style="FONT: 10pt arial"><B>To:</B> <A title=nandlals@hotmail.com href="mailto:nandlals@hotmail.com">Sharman</A> ; <A title=hgoto@usp.br href="mailto:hgoto@usp.br">Hiro Goto</A> ; <A title=elfadil_abass@yahoo.com href="mailto:elfadil_abass@yahoo.com">elfadil abass</A> </DIV>
<DIV style="FONT: 10pt arial"><B>Cc:</B> <A title=leish-l@lineu.icb.usp.br href="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</A> </DIV>
<DIV style="FONT: 10pt arial"><B>Sent:</B> Sunday, May 22, 2011 1:39 AM</DIV>
<DIV style="FONT: 10pt arial"><B>Subject:</B> Re: [Leish-l] inquiry</DIV>
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<DIV dir=ltr><FONT color=#000000 size=2 face=Arial>Dr. NL Sharma has been working on an important CL endemic area along the Satluj River valley to the south of Himalaya in India (Please correct me should I be wrong for anything I said here and below). I had the good fortune of visiting the site several years back courtesy of Dr. Sharma. </FONT></DIV>
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<DIV dir=ltr><FONT size=2 face=Arial>I believe Dr. Sharma's finding is important, since the parasites there are different from the familiar Indian L donovani in Bihar. The parasites are refratory to in vitro cultivation. They do differentiate into promastigotes and may grow a little, but can't really be subcultured to establish passageable lines. This is very much reminescent of L infantum in the Mediterranean area. I recall Dr. Sharma has also found rK39+ dogs, if I remember correctly. If so, Satluj river valley is endemic to the infantile CL.</FONT></DIV>
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<DIV dir=ltr><FONT size=2 face=Arial>Analyses of several batches of basically clinical CL samples from Dr. Sharma showed evidence of L infantum, but also L tropica as well as a mixture of the two in one sample. This is the same picture we have noted for samples from Hatay, Turkey.</FONT></DIV>
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<DIV dir=ltr><FONT face=Arial><FONT size=2><FONT size=+0>These observations make me wonder a lot about our current knowledge on the clinico-epidemiology based on data collected previously by analyses of cultured promastigotes from one or few 'representative samples'. Nowaday, technology makes it very doable to work with biological samples for Leish DNAs directly from sand flies, patients and reservoir animals.</FONT></FONT></FONT></DIV>
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<DIV dir=ltr><FONT size=2 face=Arial>KP</FONT></DIV>
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<FONT size=2 face=Tahoma><B>From:</B> leish-l-bounces@lineu.icb.usp.br on behalf of Sharman<BR><B>Sent:</B> Fri 5/20/2011 11:31 PM<BR><B>To:</B> Hiro Goto; elfadil abass<BR><B>Cc:</B> leish-l@lineu.icb.usp.br<BR><B>Subject:</B> Re: [Leish-l] inquiry<BR></FONT><BR></DIV></DIV>
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<P><FONT size=2>Dear all<BR>I agree with Hiro gito, We are working on a focus where the CL is<BR>predominantly caused by L. donovani, and the rK 39 STRIPS GIVE POSITIVE<BR>RESULTS WITH SERA as well as serous exudate from the lesion. The results are<BR>dependent upon species.<BR>NL Sharma<BR><BR>--------------------------------------------------<BR>From: "Hiro Goto" <hgoto@usp.br><BR>Sent: Wednesday, May 18, 2011 12:09 AM<BR>To: "elfadil abass" <elfadil_abass@yahoo.com><BR>Cc: <leish-l@lineu.icb.usp.br><BR>Subject: Re: [Leish-l] inquiry<BR><BR>> Dear all,<BR>> In our oppinion, DAT and rK39 for those samples are not indicated since<BR>> these tests are produced for the diagnosis of visceral leishmaniasis.<BR>> In case of tegumentary leishmaniasis, it is very appropriate the<BR>> observation of J.J. Shaw appointing species specificity of antibody<BR>> response in these cases. Titers of antibodies are in general low in these<BR>> cases therefore depending on the species, it may result negative. We have<BR>> published a review recently in Expert Rev. Anti Infect. Ther. 8(4),<BR>> 419?433 (2010), Current diagnosis and treatment of cutaneous and<BR>> mucocutaneous leishmaniasis, where we raise this point.<BR>> Hiro Goto<BR>><BR>> Citando elfadil abass <elfadil_abass@yahoo.com>:<BR>><BR>>> Dear all I would recommend using DAT and rK39 strip test to measure<BR>>> antibody<BR>>> responses and to evaluate the diagnostic efficiency for both tests in<BR>>> such group<BR>>> of patients.<BR>>><BR>>> Elfadil Abass<BR>>> ________________________________________________________________________________<BR>>> Institute of Medical Microbiology and Hospital Hygiene<BR>>> Philipps University Marburg<BR>>> BMFZ / Hans-Meerwein Straße 2<BR>>> D-35033 Marburg, Germany<BR>>> ________________________________________________________________________________<BR>>><BR>>><BR>>><BR>>><BR>>> ________________________________<BR>>> From: Nuha Nuwayri-Salti <racha@aub.edu.lb><BR>>> To: saad saad <saad1426@gmail.com>; "leish-l@lineu.icb.usp.br"<BR>>> <leish-l@lineu.icb.usp.br><BR>>> Sent: Fri, May 13, 2011 9:39:33 AM<BR>>> Subject: Re: [Leish-l] inquiry<BR>>><BR>>> Dear Saad first precaution to take is to separate your samples into<BR>>> several<BR>>> portions each (at least 5 each being no more than a few hundred(200-400)<BR>>> microliters). This is a necessary precaution to avoid freezing and<BR>>> thawing<BR>>> several times the same sample which will be the case should you do<BR>>> different<BR>>> studies at different times which is unavoidable.<BR>>><BR>>> What you can do is correlate the type(ulcerated, abscess, furuncle<BR>>> etc.. ) the<BR>>> locale, the number and age of lesions with the levels of antibody in the<BR>>> sera of<BR>>> these patgients and also monitor cell mediated immunity with leishmanin<BR>>> skin<BR>>> test!<BR>>> I have just published (in print) an article about having circulating<BR>>> parasites<BR>>> in some of these patients with apparently pure cutaneous disease. It is<BR>>> the<BR>>> first paper that revealed this fact. It would be interesting to confirm<BR>>> or<BR>>> de-confirm this fact repeating what we did.<BR>>> Best wishes<BR>>><BR>>> Nuha Nuwayri-Salti MD<BR>>><BR>>><BR>>><BR>>> AOA<BR>>> Medical Honor Society<BR>>><BR>>><BR>>><BR>>><BR>>> -----Original Message-----<BR>>> From: leish-l-bounces@lineu.icb.usp.br<BR>>> [<A href="mailto:leish-l-bounces@lineu.icb.usp.br">mailto:leish-l-bounces@lineu.icb.usp.br</A>]<BR>>> On Behalf Of saad saad<BR>>> Sent: Friday, April 29, 2011 11:23 PM<BR>>> To: leish-l@lineu.icb.usp.br<BR>>> Subject: [Leish-l] inquiry<BR>>><BR>>> Dear all<BR>>> Hi. i am Saad from Saudi Arabia. i have 60 sera from positive case of<BR>>> CL from south west of the country. I would like to have your<BR>>> recommendations to start a good research line in CL using these sera.<BR>>> Thanks in advance for your help<BR>>> _______________________________________________<BR>>> Leish-l mailing list<BR>>> Leish-l@lineu.icb.usp.br<BR>>> <A href="http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l">http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l</A><BR>>> _______________________________________________<BR>>> Leish-l mailing list<BR>>> Leish-l@lineu.icb.usp.br<BR>>> <A href="http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l">http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l</A><BR>>><BR>><BR>><BR>><BR>> Profa. Dra. Hiro Goto<BR>> Laboratório de Soroepidemiologia e Imunobiologia<BR>> Instituto de Medicina Tropical de São Paulo, USP<BR>> Av. Dr. Enéas de Carvalho Aguiar, 470, prédio II, quarto andar<BR>> 05403-000 - São Paulo, SP<BR>> Tel. +55-11-3061 7023, 3061 7056 ou 3061 7027<BR>> Fax. +55-11-3061-8270<BR>><BR>> _______________________________________________<BR>> Leish-l mailing list<BR>> Leish-l@lineu.icb.usp.br<BR>> <A href="http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l">http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l</A><BR>><BR>_______________________________________________<BR>Leish-l mailing list<BR>Leish-l@lineu.icb.usp.br<BR><A href="http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l">http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l</A><BR></FONT></P></DIV>
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