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<DIV><FONT face=Arial size=2>Hi K.P.,</FONT></DIV>
<DIV><FONT face=Arial size=2>in Cukurova region, Turkey, cutaneous L. infantum
(now it seems that we are dealing with L.donovani/L. infantum hybrid) grew
very poorely if we isolated them from patients. Only 1 of 25 isolations was
succesfull. However, the same strain (confirmed by molecular methods) grew
repeatedly and very well if we isolated them from sandflies. It might be useful
for Dr. Sharma try to get isolates from sand flies. It is very laborious but
very useful, in Cukurova we got about dozen of isolates by this method (all
identical).</FONT></DIV>
<DIV><FONT face=Arial size=2>Patients are rK39 negative, see attached
paper.</FONT></DIV>
<DIV><FONT face=Arial size=2>Best wishes</FONT></DIV>
<DIV><FONT face=Arial size=2>Petr</FONT></DIV>
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<DIV style="FONT: 10pt arial">----- Original Message ----- </DIV>
<DIV
style="BACKGROUND: #e4e4e4; FONT: 10pt arial; font-color: black"><B>From:</B>
<A title=KwangPoo.Chang@rosalindfranklin.edu
href="mailto:KwangPoo.Chang@rosalindfranklin.edu">Chang, Kwang-Poo</A> </DIV>
<DIV style="FONT: 10pt arial"><B>To:</B> <A title=nandlals@hotmail.com
href="mailto:nandlals@hotmail.com">Sharman</A> ; <A title=hgoto@usp.br
href="mailto:hgoto@usp.br">Hiro Goto</A> ; <A title=elfadil_abass@yahoo.com
href="mailto:elfadil_abass@yahoo.com">elfadil abass</A> </DIV>
<DIV style="FONT: 10pt arial"><B>Cc:</B> <A title=leish-l@lineu.icb.usp.br
href="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</A> </DIV>
<DIV style="FONT: 10pt arial"><B>Sent:</B> Sunday, May 22, 2011 1:39 AM</DIV>
<DIV style="FONT: 10pt arial"><B>Subject:</B> Re: [Leish-l] inquiry</DIV>
<DIV><BR></DIV>
<DIV id=idOWAReplyText41530 dir=ltr>
<DIV dir=ltr><FONT face=Arial color=#000000 size=2>Dr. NL Sharma has been
working on an important CL endemic area along the Satluj River valley to
the south of Himalaya in India (Please correct me should I be wrong for
anything I said here and below). I had the good fortune of visiting the site
several years back courtesy of Dr. Sharma. </FONT></DIV>
<DIV dir=ltr><FONT face=Arial size=2></FONT> </DIV>
<DIV dir=ltr><FONT face=Arial size=2>I believe Dr. Sharma's finding is
important, since the parasites there are different from the familiar Indian L
donovani in Bihar. The parasites are refratory to in vitro cultivation. They
do differentiate into promastigotes and may grow a little, but can't
really be subcultured to establish passageable lines. This is very much
reminescent of L infantum in the Mediterranean area. I recall Dr. Sharma has
also found rK39+ dogs, if I remember correctly. If so, Satluj river
valley is endemic to the infantile CL.</FONT></DIV>
<DIV dir=ltr><FONT face=Arial size=2></FONT> </DIV>
<DIV dir=ltr><FONT face=Arial size=2>Analyses of several batches of basically
clinical CL samples from Dr. Sharma showed evidence of L infantum, but also L
tropica as well as a mixture of the two in one sample. This is the same
picture we have noted for samples from Hatay, Turkey.</FONT></DIV>
<DIV dir=ltr><FONT face=Arial><FONT size=2><FONT
size=+0></FONT></FONT></FONT> </DIV>
<DIV dir=ltr><FONT face=Arial><FONT size=2><FONT size=+0>These observations
make me wonder a lot about our current knowledge on the clinico-epidemiology
based on data collected previously by analyses of cultured promastigotes
from one or few 'representative samples'.
Nowaday, technology makes it very doable to work with biological
samples for Leish DNAs directly from sand flies, patients and reservoir
animals.</FONT></FONT></FONT></DIV>
<DIV dir=ltr><FONT face=Arial size=2></FONT> </DIV>
<DIV dir=ltr><FONT face=Arial size=2>KP</FONT></DIV>
<DIV dir=ltr><FONT face=Arial color=#000000 size=2></FONT> </DIV></DIV>
<DIV id=idSignature79278 dir=ltr>
<DIV> </DIV>
<DIV>
<HR tabIndex=-1>
<FONT face=Tahoma size=2><B>From:</B> leish-l-bounces@lineu.icb.usp.br on
behalf of Sharman<BR><B>Sent:</B> Fri 5/20/2011 11:31 PM<BR><B>To:</B> Hiro
Goto; elfadil abass<BR><B>Cc:</B> leish-l@lineu.icb.usp.br<BR><B>Subject:</B>
Re: [Leish-l] inquiry<BR></FONT><BR></DIV></DIV>
<DIV>
<P><FONT size=2>Dear all<BR>I agree with Hiro gito, We are working on a focus
where the CL is<BR>predominantly caused by L. donovani, and the rK 39 STRIPS
GIVE POSITIVE<BR>RESULTS WITH SERA as well as serous exudate from the lesion.
The results are<BR>dependent upon species.<BR>NL
Sharma<BR><BR>--------------------------------------------------<BR>From:
"Hiro Goto" <hgoto@usp.br><BR>Sent: Wednesday, May 18, 2011 12:09
AM<BR>To: "elfadil abass" <elfadil_abass@yahoo.com><BR>Cc:
<leish-l@lineu.icb.usp.br><BR>Subject: Re: [Leish-l] inquiry<BR><BR>>
Dear all,<BR>> In our oppinion, DAT and rK39 for those samples are not
indicated since<BR>> these tests are produced for the diagnosis of
visceral leishmaniasis.<BR>> In case of tegumentary leishmaniasis, it
is very appropriate the<BR>> observation of J.J. Shaw appointing species
specificity of antibody<BR>> response in these cases. Titers of antibodies
are in general low in these<BR>> cases therefore depending on the
species, it may result negative. We have<BR>> published a review
recently in Expert Rev. Anti Infect. Ther. 8(4),<BR>> 419?433 (2010),
Current diagnosis and treatment of cutaneous and<BR>> mucocutaneous
leishmaniasis, where we raise this point.<BR>> Hiro Goto<BR>><BR>>
Citando elfadil abass <elfadil_abass@yahoo.com>:<BR>><BR>>>
Dear all I would recommend using DAT and rK39 strip test to
measure<BR>>> antibody<BR>>> responses and to evaluate the
diagnostic efficiency for both tests in<BR>>> such
group<BR>>> of patients.<BR>>><BR>>> Elfadil
Abass<BR>>>
________________________________________________________________________________<BR>>>
Institute of Medical Microbiology and Hospital Hygiene<BR>>> Philipps
University Marburg<BR>>> BMFZ / Hans-Meerwein Straße 2<BR>>>
D-35033 Marburg, Germany<BR>>>
________________________________________________________________________________<BR>>><BR>>><BR>>><BR>>><BR>>>
________________________________<BR>>> From: Nuha Nuwayri-Salti
<racha@aub.edu.lb><BR>>> To: saad saad <saad1426@gmail.com>;
"leish-l@lineu.icb.usp.br"<BR>>>
<leish-l@lineu.icb.usp.br><BR>>> Sent: Fri, May 13, 2011 9:39:33
AM<BR>>> Subject: Re: [Leish-l] inquiry<BR>>><BR>>> Dear
Saad first precaution to take is to separate your samples into<BR>>>
several<BR>>> portions each (at least 5 each being no more than a few
hundred(200-400)<BR>>> microliters). This is a necessary precaution to
avoid freezing and<BR>>> thawing<BR>>> several times the same
sample which will be the case should you do<BR>>> different<BR>>>
studies at different times which is unavoidable.<BR>>><BR>>> What
you can do is correlate the type(ulcerated, abscess,
furuncle<BR>>> etc.. ) the<BR>>> locale, the number and age
of lesions with the levels of antibody in the<BR>>> sera
of<BR>>> these patgients and also monitor cell mediated immunity with
leishmanin<BR>>> skin<BR>>> test!<BR>>> I have just
published (in print) an article about having circulating<BR>>>
parasites<BR>>> in some of these patients with apparently pure cutaneous
disease. It is<BR>>> the<BR>>> first paper that revealed this
fact. It would be interesting to confirm<BR>>> or<BR>>> de-confirm
this fact repeating what we did.<BR>>> Best
wishes<BR>>><BR>>> Nuha Nuwayri-Salti
MD<BR>>><BR>>><BR>>><BR>>> AOA<BR>>> Medical
Honor Society<BR>>><BR>>><BR>>><BR>>><BR>>>
-----Original Message-----<BR>>> From:
leish-l-bounces@lineu.icb.usp.br<BR>>> [<A
href="mailto:leish-l-bounces@lineu.icb.usp.br">mailto:leish-l-bounces@lineu.icb.usp.br</A>]<BR>>>
On Behalf Of saad saad<BR>>> Sent: Friday, April 29, 2011 11:23
PM<BR>>> To: leish-l@lineu.icb.usp.br<BR>>> Subject: [Leish-l]
inquiry<BR>>><BR>>> Dear all<BR>>> Hi. i am Saad from Saudi
Arabia. i have 60 sera from positive case of<BR>>> CL from south west of
the country. I would like to have your<BR>>> recommendations to start a
good research line in CL using these sera.<BR>>> Thanks in advance for
your help<BR>>>
_______________________________________________<BR>>> Leish-l mailing
list<BR>>> Leish-l@lineu.icb.usp.br<BR>>> <A
href="http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l">http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l</A><BR>>>
_______________________________________________<BR>>> Leish-l mailing
list<BR>>> Leish-l@lineu.icb.usp.br<BR>>> <A
href="http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l">http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l</A><BR>>><BR>><BR>><BR>><BR>>
Profa. Dra. Hiro Goto<BR>> Laboratório de Soroepidemiologia e
Imunobiologia<BR>> Instituto de Medicina Tropical de São Paulo, USP<BR>>
Av. Dr. Enéas de Carvalho Aguiar, 470, prédio II, quarto andar<BR>>
05403-000 - São Paulo, SP<BR>> Tel. +55-11-3061 7023, 3061 7056 ou 3061
7027<BR>> Fax. +55-11-3061-8270<BR>><BR>>
_______________________________________________<BR>> Leish-l mailing
list<BR>> Leish-l@lineu.icb.usp.br<BR>> <A
href="http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l">http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l</A><BR>><BR>_______________________________________________<BR>Leish-l
mailing list<BR>Leish-l@lineu.icb.usp.br<BR><A
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