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<p class="MsoNormal">Dear all, </p>
<p class="MsoNormal">I have cloned 117 bp Tandem Repeats (6.3 copies) of k39
kinesin related gene of L.donovani strain from Sudan in pcDNA. Know, I would like
to purify the protein. Nucleotide sequence analysis showed high identities (over
90%) with other published kinesin. Does it make a sense at protein expression
level? Regarding protein purification, is the so called IMPACT has advantage
over the classical His Tag vector? What do you think about a combination strategy
of both purification methods?</p>
<p class="MsoNormal">Best regards,,</p>
<div> </div>Elfadil Abass<br>________________________________________________________________________________<br>Institute of Medical Microbiology and Hospital Hygiene<br>Philipps University Marburg<br>BMFZ / Hans-Meerwein Straße 2<br>D-35033 Marburg, Germany<br>________________________________________________________________________________<div><br></div>
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