[Leish-l] --- Marqué SPAM --- Modifié par ATI --- Growth curve in promastigote culture

[Aoun Karim]

Dear Nayore, 
In our expérience We prefer growing the Leishmania promastigotes in culture flasks. 
Best. Karim. 
  
  
   
   

-----Message original-----

De: Nayore <nayoretk at gmail.com>
à: leish-l <leish-l at lineu.icb.usp.br>
Envoyé: lundi 14 août 2023 16:42 CET
Sujet : --- Marqué SPAM --- Modifié par ATI --- [Leish-l] Growth curve in promastigote culture

Good afternoon,

My name is Nayore, I am a doctoral student and researcher at UFSCar and I
work with Leishmania infantum promastigotes culture. I have doubts about
how to perform the growth curve, should I do it in 12 well plate or in a
culture flask?

I performed this type of count for other strains, however I was performing
the plate count during 10 days, starting from a concentration of 2.0x10e5
promastigotes/mL in first day. Recently I was asked why I was making it on
a well and not in a flask.

I would like to solve this question about which is the best way to make the
curve in a well containing 2.0x10e5 promastigotes/2 mL or in a culture
flask?

thank you for your attention

Best Regards
Nayore Tamie Takamiya

-- 
*Nayore Tamie Takamiya*
Bióloga/ Doutoranda no Programa de Pós-graduação em Genética Evolutiva e
Biologia Molecular
Universidade Federal de São Carlos (UFSCar)
Centro de Ciências Biológicas e da Saúde (CCBS)
Departamento de Genética e Evolução (DGE)
Rod. Washington Luís, Km 235 Caixa Postal 676
CEP. 13565-905 - São Carlos - SP - Brasil
http://lattes.cnpq.br/6422940683742319
_______________________________________________
Leish-l mailing list
Leish-l at lineu.icb.usp.br
http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l

-- 
This email was sent by icb.usp.br