[Leish-l] [Possivel Spam] Fw: WL7 RT Towards a consensus on Leishmania identification and characterization

[jeffrey shaw]

________________________________
From: Maxy De los Santos <dmaxy at hotmail.com>
Sent: 29 July 2022 23:11
To: jeffrey shaw <jayusp at hotmail.com>
Subject: RE: [Leish-l] WL7 RT Towards a consensus on Leishmania identification and characterization

Dear Dr. Shaw

I am a molecular biologist that works in leishmaniasis, principally cutaneous for NAMRU-6, Lima.

As I discussed months ago with Dr. Cupolillo, we need to reinforce the terms about the gold standard for leishmaniasis diagnosis. Microscopy is a good approach but false negatives can occur. Culture isolation for MLEE is hard to work and sustain due to lab capacities. Molecular markers like Hsp70 could work but is hard to discriminate species through PCR-RFLP. Other technics as HRM have problems in discriminating narrowed related species such as L. peruviana and L. braziliensis for example. TaqMan probes could work better if to get decrease the costs of infrastructure.

We developed a Nested RT PCR to determine species, but we found problems associated with parasite load that could be changing between the type of samples. Quality of DNA and time of disease could be involved too.

So we need to work on a tentative flowchart for overcoming multiple troubles in diagnostic; since increasing sanitary personnel capabilities to discriminate leishmaniasis from other pathologies by clinical first filters, generalized use of less invasive and no painful sampling, overcome the presence of human DNA contamination, selection of "easy to work, easy to use" markers to enhance molecular assays, enhance the DNA sequencing to have in the future a database that reflects the genetic diversity and permit rapid identification and distribution assesment of the parasites.

This first approaches can facilitate timely treatment, surveillance, studies in vectors and reservoirs, new studies and publications.

Our institutions need leaderships focus in more collaborative efforts, where the ideas do not stay in papers or good intentions. COVID-19 pandemic has demonstrated that efforts in vaccines and rapid test can be better than anybody dream! And lab research need more recognition and funds to get it.

I hope some of my ideas can be discussed during the event.

Very respectfully

Maxy De los Santos Delgado

________________________________
De: leish-l-bounces at lineu.icb.usp.br <leish-l-bounces at lineu.icb.usp.br> en nombre de jeffrey shaw <jayusp at hotmail.com>
Enviado: viernes, 22 de julio de 2022 7:02
Para: Leish-L <leish-l at lineu.icb.usp.br>
Asunto: [Leish-l] WL7 RT Towards a consensus on Leishmania identification and characterization

There is an important round table at WL7 on “Towards a consensus on Leishmania identification and characterization. Molecular markers for strain typing of Leishmania species which are truly useful and in which regions or conditions”.

If you have comments or ideas on this subject, please send them to Leish-L as soon as possible.



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