From chncosta at gmail.com Wed Nov 14 10:36:21 2018 From: chncosta at gmail.com (Carlos Costa) Date: Wed, 14 Nov 2018 09:36:21 -0300 Subject: [Leish-l] silent Message-ID: Why leishmaniacs are so silent? Nothing to talk about? Jorge???!!! Cheers, Carlos. -- Carlos H. N. Costa, MD, DSc. President Instituto de Doen?as do Sert?o *Instituto de Doen?as Tropicais Natan Portella* *Centro de Ci?ncias da Sa?de da Universidade Federal do Piau?* *Rua Artur de Vasconcelos 151-Sul* *64001-450 Teresina-PI* *Brazil* Telephones: +55 86 3222-4377 (W), +55 86 3221-3062 (W), +55 86 3237-1075 (H). Aviso: As informa??es contidas nesta mensagem s?o CONFIDENCIAIS, protegidas pelo sigilo legal, por direitos autorais e destinadas exclusivamente ? pessoa ou organiza??o para a qual a mensagem foi destinada. Warning: This message is meant only for the intended recipient of the transmission. It is forbidden any unauthorized use, alteration, reproduction, and distribution. If you are not the correct recipient, please notify us immediately by return e-mail and delete this message from your system. From chncosta at gmail.com Thu Nov 15 14:59:08 2018 From: chncosta at gmail.com (Carlos Costa) Date: Thu, 15 Nov 2018 16:59:08 -0000 Subject: [Leish-l] Fwd: silent In-Reply-To: References: Message-ID: Dear KP, Yes, you are right. Let me start it with a very provocative issue. Months ago, we talked about getting a report about Dr. Meta Roenstemberg's proposal of "controlled human infection model" applied to kala-azar, to be published in the Newsletter of the Brazilian Society of Tropical Medicine. I have not done so due to a high demand for tasks, but also because I still hesitate when proposing it to kala-azar due to my concerns with the toxicity of treatment. I would be more encouraged to explore the subject if we get comments from our fellows leishmaniacs. I include below the link for Dr. Rostemberg's previously published synthesis of the last year meeting taken in Leiden for malaria to see what is the community thoughts on the subject. https://reader.elsevier.com/reader/sd/pii/S0264410X17315220?token=1DF2514CC4E49122BAC6002691D4F231218657AEEF392C4D3888953809D3FD7E4C84603E7313C75E9B7B096307DAE0A8 Cheers, Carlos. Em qua, 14 de nov de 2018 ?s 18:13, Kwang-Poo Chang < kwangpoo.chang at rosalindfranklin.edu> escreveu: > Dear Carlos, > > Just wonder which Jorge are you addressing for you question of why > leishmaniacs are so "silent" in your message ? Why don't you break the > silence by speaking up ? > > KP > > On Wed, Nov 14, 2018 at 2:41 PM Carlos Costa wrote: > >> Why leishmaniacs are so silent? Nothing to talk about? Jorge???!!! >> Cheers, >> Carlos. >> >> -- >> Carlos H. N. Costa, MD, DSc. >> President >> Instituto de Doen?as do Sert?o >> *Instituto de Doen?as Tropicais Natan Portella* >> >> *Centro de Ci?ncias da Sa?de da Universidade Federal do Piau?* >> *Rua Artur de Vasconcelos 151-Sul* >> *64001-450 Teresina-PI* >> *Brazil* >> Telephones: +55 86 3222-4377 (W), >> +55 86 3221-3062 (W), >> +55 86 3237-1075 (H). >> >> >> Aviso: As informa??es contidas nesta mensagem s?o CONFIDENCIAIS, >> protegidas >> pelo sigilo legal, por direitos autorais e destinadas exclusivamente ? >> pessoa ou organiza??o para a qual a mensagem foi destinada. >> Warning: This message is meant only for the intended recipient of the >> transmission. It is forbidden any unauthorized use, alteration, >> reproduction, and distribution. If you are not the correct recipient, >> please notify us immediately by return e-mail and delete this message from >> your system. >> _______________________________________________ >> Leish-l mailing list >> Leish-l at lineu.icb.usp.br >> http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l >> >> -- >> This email was sent by icb.usp.br >> > -- Carlos H. N. Costa, MD, DSc. President Instituto de Doen?as do Sert?o *Instituto de Doen?as Tropicais Natan Portella* *Centro de Ci?ncias da Sa?de da Universidade Federal do Piau?* *Rua Artur de Vasconcelos 151-Sul* *64001-450 Teresina-PI* *Brazil* Telephones: +55 86 3222-4377 (W), +55 86 3221-3062 (W), +55 86 3237-1075 (H). Aviso: As informa??es contidas nesta mensagem s?o CONFIDENCIAIS, protegidas pelo sigilo legal, por direitos autorais e destinadas exclusivamente ? pessoa ou organiza??o para a qual a mensagem foi destinada. Warning: This message is meant only for the intended recipient of the transmission. It is forbidden any unauthorized use, alteration, reproduction, and distribution. If you are not the correct recipient, please notify us immediately by return e-mail and delete this message from your system. -- Carlos H. N. Costa, MD, DSc. President Instituto de Doen?as do Sert?o *Instituto de Doen?as Tropicais Natan Portella* *Centro de Ci?ncias da Sa?de da Universidade Federal do Piau?* *Rua Artur de Vasconcelos 151-Sul* *64001-450 Teresina-PI* *Brazil* Telephones: +55 86 3222-4377 (W), +55 86 3221-3062 (W), +55 86 3237-1075 (H). Aviso: As informa??es contidas nesta mensagem s?o CONFIDENCIAIS, protegidas pelo sigilo legal, por direitos autorais e destinadas exclusivamente ? pessoa ou organiza??o para a qual a mensagem foi destinada. Warning: This message is meant only for the intended recipient of the transmission. It is forbidden any unauthorized use, alteration, reproduction, and distribution. If you are not the correct recipient, please notify us immediately by return e-mail and delete this message from your system. From biomoljazz at gmail.com Thu Nov 15 17:05:59 2018 From: biomoljazz at gmail.com (Jorge Arevalo) Date: Thu, 15 Nov 2018 19:05:59 -0000 Subject: [Leish-l] silent In-Reply-To: References: Message-ID: Dear Carlos: Perhaps we are in our quiescent stage. The scarce environment ($) have turned off our translation machinery. Cheers. Jorge El mi?., 14 nov. 2018 a las 15:42, Carlos Costa () escribi?: > Why leishmaniacs are so silent? Nothing to talk about? Jorge???!!! > Cheers, > Carlos. > > -- > Carlos H. N. Costa, MD, DSc. > President > Instituto de Doen?as do Sert?o > *Instituto de Doen?as Tropicais Natan Portella* > > *Centro de Ci?ncias da Sa?de da Universidade Federal do Piau?* > *Rua Artur de Vasconcelos 151-Sul* > *64001-450 Teresina-PI* > *Brazil* > Telephones: +55 86 3222-4377 (W), > +55 86 3221-3062 (W), > +55 86 3237-1075 (H). > > > Aviso: As informa??es contidas nesta mensagem s?o CONFIDENCIAIS, protegidas > pelo sigilo legal, por direitos autorais e destinadas exclusivamente ? > pessoa ou organiza??o para a qual a mensagem foi destinada. > Warning: This message is meant only for the intended recipient of the > transmission. It is forbidden any unauthorized use, alteration, > reproduction, and distribution. If you are not the correct recipient, > please notify us immediately by return e-mail and delete this message from > your system. > _______________________________________________ > Leish-l mailing list > Leish-l at lineu.icb.usp.br > http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l > > -- > This email was sent by icb.usp.br > From mikko_aalto at yahoo.com Fri Nov 23 13:30:13 2018 From: mikko_aalto at yahoo.com (Mikko Aalto) Date: Fri, 23 Nov 2018 15:30:13 +0000 (UTC) Subject: [Leish-l] Simple method to promastigote culture from peripheral blood References: <66376722.5623273.1542987013734.ref@mail.yahoo.com> Message-ID: <66376722.5623273.1542987013734@mail.yahoo.com> Dear All O meus colegas Corresponder em ingles e em portugues. Can anyone advice a simple method how to culture promastigotes from human peripheral blood? I have used promastigote culture as diagnostic method for rK39 RDT negative Visceral Leishmaniasis patients. I have inoculated spleen and bone marrow aspirates to 10ml vials with autoclaved house made medium consisting of Tryptone, Yeast Extract, NaCl sealed with rubber stoppers and 5% human blood is added there after, kept at 80-100C for 15 minutes to inactivate inhibitory components. Just before inoculation I have added 1-2ml 10% filter sterilized human urine as an overlay, serving also to dilute complement and other inhibitory components in the maybe around 50?l aspirate. With strict sterility antibiotics have not been necessary. Incubated in a cooling incubator at 25C, the living motile promastigotes have been visible in wet preparation in 48 hours to 30days. But only around 70% of cultures have been positive. Now I would like to start doing promastigote cultures also from peripheral blood. The 2002 method by Shyam Sundar* and M. Rai (Laboratory Diagnosis of Visceral Leishmaniasis) seems feasible to my very basic conditions.? "Aseptically collected blood (1 to 2 ml) is diluted with 10 ml of citrated saline, and the cellular deposit obtained after centrifugation is inoculated in culture media." But the recently published methods using buffy coats and micotiter plates etc seem too complicated to simple conditions. So I would like to hear advices on simple but good techniques to do promastigote cultures from patients' peripheral blood. And also if Streptokinase might help to eliminate complement and other inhibitory factors, without inhibiting amastigotes and promastigotes. Yours Mikko Dr Mikko Aalto Bosaso General Hospital Bosaso Somalia From chncosta at gmail.com Sat Nov 24 10:06:13 2018 From: chncosta at gmail.com (Carlos Costa) Date: Sat, 24 Nov 2018 09:06:13 -0300 Subject: [Leish-l] burqa Message-ID: Recently, it came up in my mind that the use of burqa might be associated with the protection against cutaneous leishmaniasis in the Middle-East. I am pretty sure that I heard from someone or read somewhere but I cannot recall the source. Then I asked my Prof. Ali Khamesipour, from Tehran, and to Prof. Jeff Shaw but they do not know anything about the subject. Finally, I entered in PubMed and found only the attached article by Al Aboud et al with a quote that burqa might be protective against leishmaniasis. The issue is quite interesting. So, I ask the colleagues who know something or that want to express their thought about the issue to share with our community. Cheers, Carlos. -- Carlos H. N. Costa, MD, DSc. President Instituto de Doen?as do Sert?o *Instituto de Doen?as Tropicais Natan Portella* *Centro de Ci?ncias da Sa?de da Universidade Federal do Piau?* *Rua Artur de Vasconcelos 151-Sul* *64001-450 Teresina-PI* *Brazil* Telephones: +55 86 3222-4377 (W), +55 86 3221-3062 (W), +55 86 3237-1075 (H). Aviso: As informa??es contidas nesta mensagem s?o CONFIDENCIAIS, protegidas pelo sigilo legal, por direitos autorais e destinadas exclusivamente ? pessoa ou organiza??o para a qual a mensagem foi destinada. Warning: This message is meant only for the intended recipient of the transmission. It is forbidden any unauthorized use, alteration, reproduction, and distribution. If you are not the correct recipient, please notify us immediately by return e-mail and delete this message from your system. From mikko_aalto at yahoo.com Wed Nov 28 06:22:58 2018 From: mikko_aalto at yahoo.com (Mikko Aalto) Date: Wed, 28 Nov 2018 08:22:58 +0000 (UTC) Subject: [Leish-l] Fw: Simple method to promastigote culture from peripheral blood References: <1173024038.8088905.1543393378476.ref@mail.yahoo.com> Message-ID: <1173024038.8088905.1543393378476@mail.yahoo.com> Dear All Have You already checked this to be posted on leishnet? And I have not found the discussion forum on leishnet. Can You send a link to it? Yours Mikko --- On Fri, 11/23/18, Mikko Aalto wrote: Dear All Os meus colegas Corresponder em ingles e em portugues. Can anyone advice a simple method how to culture promastigotes from human peripheral blood? I have used promastigote culture as diagnostic method for rK39 RDT negative Visceral Leishmaniasis patients. I have inoculated spleen and bone marrow aspirates to 10ml vials with autoclaved house made medium consisting of Tryptone, Yeast Extract, NaCl sealed with rubber stoppers and 5% human blood is added there after, kept at 80-100C for 15 minutes to inactivate inhibitory components. Just before inoculation I have added 1-2ml 10% filter sterilized human urine as an overlay, serving also to dilute complement and other inhibitory components in the maybe around 50?l aspirate. With strict sterility antibiotics have not been necessary. Incubated in a cooling incubator at 25C, the living motile promastigotes have been visible in wet preparation in 48 hours to 30days. But only around 70% of cultures have been positive. Now I would like to start doing promastigote cultures also from peripheral blood. The 2002 method by Shyam Sundar* and M. Rai (Laboratory Diagnosis of Visceral Leishmaniasis) seems feasible to my very basic conditions. "Aseptically collected blood (1 to 2 ml) is diluted with 10 ml of citrated saline, and the cellular deposit obtained after centrifugation is inoculated in culture media." But the recently published methods using buffy coats and micotiter plates etc seem too complicated to simple conditions. So I would like to hear advices on simple but good techniques to do promastigote cultures from patients' peripheral blood. And also if Streptokinase might help to eliminate complement and other inhibitory factors, without inhibiting amastigotes and promastigotes. Yours Mikko Dr Mikko Aalto Bosaso General Hospital Bosaso Somalia