[Leish-l] Status of PCR for CL diagnosis

[Noris Rodriguez]

Dear Dr. Valdivia



In Venezuela, we are using PCR for diagnosis since 1990 and also
 microscopy, but we are doing PCR with species specific oligonucleotides ,
in order to do both: diagnosis and species identification just in one PCR
reaction.

I am agree to have a meeting in Toledo, in order to make a regional project
to evalauate all the PCR protocols  used in each counntry.



Best Regards



Dra. Noris Rodriguez

Directora

Instituto de Biomedicina "Dr. Jacinto Convit

Facultad de Medicina

Universidad Central de Venezuela

Telefax: 0212 8648624




2017-04-12 9:04 GMT-04:30 Kwang-Poo Chang <
kwangpoo.chang at rosalindfranklin.edu>:

> In sanliurfa, Turkey, dermatologists placed lesion aspirates on glass
> slides and examined the wet mount preparations for the presence of
> amastigotes as a quick way of diagnosis. Remaining samples were used for
> culture and/or PCR, if necessary.
>
> This is doable if amastigotes are abundant and large in size.
>
> Just pass on the information for your reference.
>
> KP
>
> On Wed, Apr 5, 2017 at 8:40 AM, Hugo Valdivia <hvalrod at hotmail.com> wrote:
>
> > Thank you all for your thoughts and insights, it is fantastic to see all
> > the interaction in the Leish community,
> >
> >
> > I am fully aware of the advantages of PCR and the clear need of
> > standardization in the region for CL diagnosis. I hope that this issue
> will
> > be discussed on WorldLeish and could result in better diagnostics for the
> > people who are most affected by this disease.
> >
> >
> > Thank you,
> >
> > Hugo Valdivia,
> >
> >
> >
> >
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