From skarora_in at yahoo.com Tue Jul 25 01:53:32 2017 From: skarora_in at yahoo.com (Sunil Arora) Date: Tue, 25 Jul 2017 04:53:32 +0000 (UTC) Subject: [Leish-l] Status of PCR for CL diagnosis In-Reply-To: References: Message-ID: <1190930016.173549.1500958412328@mail.yahoo.com> Dear allI agree with Dr Eslami, the issue of usability and authenticity of using molecular assays like PCR for the detection of Leishmanial infections need a thorough discussion (may be a meeting) to remove the ambiguities..regardssunil ---------------------------------------------------------------Dr Sunil K.Arora Professor Head-Dept. of Translational & Regenerative Medicine,?Councillor- Federation of Immunological Societies of Asia-oceania (FIMSA)General Secretary (Research)- The Cytometry Society of India (TCS)?Department of Immunopathology PGIMER, Chandigarh-160 012 Ph.: 0091-172-2755192 (Off) FAX: 0091-172-2744401, 2745078 Ph:+91-172-4666087 (Res); 9872866087 (cell) email: skarora_in at yahoo.com ; skarorain at gmail.com On Monday 24 July 2017, 6:35:32 PM IST, Yadon, Dra. Zaida (AFT) wrote: I would like to share with the link to the information publishes by PAHO on the? International? initiative for the standardization and validation of PCR for CL diagnosis and disease management across laboratories and Latin American and Caribbean countries Web Linlks Espanol http://www.paho.org/hq/index.php?option=com_content&view=article&id=13405&Itemid=42367?=es English http://www.paho.org/hq/index.php?option=com_content&view=article&id=13405&Itemid=42367?=en Links to PDF: English http://www2.paho.org/hq/index.php?option=com_docman&task=doc_view&gid=40483&Itemid=270 Espa?ol http://www2.paho.org/hq/index.php?option=com_docman&task=doc_view&gid=40486&Itemid=270 Portuguese http://www2.paho.org/hq/index.php?option=com_docman&task=doc_view&gid=40483&Itemid=270 Dr. Zaida Yadon Regional Advisor on Communicable Diseases Research Communicable Diseases and Health Analysis Department Pan American Health Organization Tel.: + 55 21 3661 9056 - VOIP 49056 yadonzai at paho.org -----Original Message----- From: leish-l-bounces at lineu.icb.usp.br [mailto:leish-l-bounces at lineu.icb.usp.br] On Behalf Of Noris Rodriguez Sent: Sunday, May 07, 2017 4:36 PM To: Kwang-Poo Chang Cc: Leish-L Subject: Re: [Leish-l] Status of PCR for CL diagnosis Dear Dr. Valdivia In Venezuela, we are using PCR for diagnosis since 1990 and also? microscopy, but we are doing PCR with species specific oligonucleotides , in order to do both: diagnosis and species identification just in one PCR reaction. I am agree to have a meeting in Toledo, in order to make a regional project to evalauate all the PCR protocols? used in each counntry. Best Regards Dra. Noris Rodriguez Directora Instituto de Biomedicina "Dr. Jacinto Convit Facultad de Medicina Universidad Central de Venezuela Telefax: 0212 8648624 2017-04-12 9:04 GMT-04:30 Kwang-Poo Chang < kwangpoo.chang at rosalindfranklin.edu>: > In sanliurfa, Turkey, dermatologists placed lesion aspirates on glass > slides and examined the wet mount preparations for the presence of > amastigotes as a quick way of diagnosis. Remaining samples were used > for culture and/or PCR, if necessary. > > This is doable if amastigotes are abundant and large in size. > > Just pass on the information for your reference. > > KP > > On Wed, Apr 5, 2017 at 8:40 AM, Hugo Valdivia wrote: > > > Thank you all for your thoughts and insights, it is fantastic to see > > all the interaction in the Leish community, > > > > > > I am fully aware of the advantages of PCR and the clear need of > > standardization in the region for CL diagnosis. I hope that this > > issue > will > > be discussed on WorldLeish and could result in better diagnostics > > for the people who are most affected by this disease. > > > > > > Thank you, > > > > Hugo Valdivia, > > > > > > > > > > _______________________________________________ > > Leish-l mailing list > > Leish-l at lineu.icb.usp.br > > http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l > > > > -- > > This email was sent by icb.usp.br > > > _______________________________________________ > Leish-l mailing list > Leish-l at lineu.icb.usp.br > http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l > > -- > This email was sent by icb.usp.br > _______________________________________________ Leish-l mailing list Leish-l at lineu.icb.usp.br http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l -- This email was sent by icb.usp.br IMPORTANT: This transmission is for use by the intended recipient and it may contain privileged, proprietary or confidential information. If you are not the intended recipient or a person responsible for delivering this transmission to the intended recipient, you may not disclose, copy or distribute this transmission or take any action in reliance on it. If you received this transmission in error, please dispose of and delete this transmission. Thank you. _______________________________________________ Leish-l mailing list Leish-l at lineu.icb.usp.br http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l -- This email was sent by icb.usp.br From chncosta at gmail.com Wed Jul 26 10:54:21 2017 From: chncosta at gmail.com (Carlos Costa) Date: Wed, 26 Jul 2017 10:54:21 -0300 Subject: [Leish-l] Fwd: infectivity or infectiousness? In-Reply-To: References: Message-ID: Fernando Narvaez's response sounds to be the best about the issue of infectivity versus infectiousness. ---------- Forwarded message ---------- From: Fernando Andrade Narvaez Date: 2017-07-25 19:37 GMT-03:00 Subject: Re: [Leish-l] infectivity or infectiousness? To: Carlos Costa Dear Carlos, Please review the Biology Online Dictionary. I agree that is very important to have a clear idea of their meaning and knowledge to employ them accurately. *Infectivity*: is related to the causative infectious agent, in case of leishmaniacs we are talking of *Leishmania* spp. It is usually used to talk about the capability of *Leishmania* spp. to enter the macrophages and colonize them, therefore entering, surviving and multiplying in a susceptible host. Thus is used to talk of the ability of the pathogen (your number 3 option). *Infectiousness* "the state or quality of being infectious" derive from *Infectious* term used to refer an *infectious* disease (able to be passed from one person,anmal to other in vector borne disease through the insect vector), an *infectious* patient (able to pass a diseases of one person or animal to other), *infectious *period (used mainly for viral diseases). Thus infectiousness is used to talk about the host or sand fly ability to pass or transfer (options 1 & 2). Infectiosity is a synonym of infectiousness. Best regards, Dr. Fernando J. Andrade Narv?ez Laboratorio de Inmunolog?a Centro de Investigaciones Regionales "Dr. Hideyo Noguchi" Universidad Aut?noma de Yucat?n Ave. Itz?es No. 490x59 CP 97000 M?rida, Yucat?n, M?xico Tel.: 999 924-6412 ext. 1156 y 1155 Fax: 999 923-6120 E-mails: anarvaez at correo.uady.mx, fandrade47 at gmail.com Fernando J. Andrade-Narvaez, MD, PhD Immunology Laboratory Centro de Investigaciones Regionales "Dr. Hideyo Noguchi" Universidad Aut?noma de Yucat?n, Ave. Itz?es No. 490x59 CP 97000 M?rida, Yucat?n, M?xico Tel.: 52 999 924-6412 ext. 1156 & 1155 Fax: 52 999 923-6120 E-mails: anarvaez at correo.uady.mx, fandrade47 at gmail.com 2017-06-24 8:57 GMT-05:00 Carlos Costa : > sorry, the last one should be infectiosity (wiki). > > 2017-06-24 10:21 GMT-03:00 Carlos Costa : > > > Dear fellows, > > > > Are there any hints on where or when to use infectivity, infectiousness > or > > infectiousness? > > 1) Talking about the host ability to transmit a pathogen. > > 2) Talking about the sand fly ability to transmit a pathogen > > 3) Talking about the pathogen ability to be transmitted. > > > > Are they synonyms? Can we use any form in any situation? > > > > I looked at the web but could not find anything sharp about this. > > > > Cheers, > > > > Carlos. > > > > -- > > Carlos H. N. Costa, MD, DSc. > > Chefe/Head > > Departamento de Medicina Comunit?ria > > Centro de Ci?ncias da Sa?de > > Coordenador/Coordinator > > Laborat?rio de Leishmanioses - LabLeish > > Instituto de Doen?as Tropicais Natan Portella > > Universidade Federal do Piau? > > Rua Artur de Vasconcelos 151-Sul > > 64001-450 Teresina-PI > > Brazil > > Telephones: +55 86 3222-4377 <+55%2086%203222-4377> > <+55%2086%203222-4377> (W), > > +55 86 3221-3062 <+55%2086%203221-3062> <+55%2086%203221-3062> (W), > > +55 86 3237-1075 <+55%2086%203237-1075> <+55%2086%203237-1075> (R). > > > > > > Aviso: As informa??es contidas nesta mensagem s?o CONFIDENCIAIS, > > protegidas pelo sigilo legal, por direitos autorais e destinadas > > exclusivamente ? pessoa ou organiza??o para a qual a mensagem foi > destinada. > > Warning: This message is meant only for the intended recipient of the > > transmission. It is forbidden any unauthorized use, alteration, > > reproduction, and distribution. If you are not the correct recipient, > > please notify us immediately by return e-mail and delete this message > from > > your system. > > > > > > -- > Carlos H. N. Costa, MD, DSc. > Chefe/Head > Departamento de Medicina Comunit?ria > Centro de Ci?ncias da Sa?de > Coordenador/Coordinator > Laborat?rio de Leishmanioses - LabLeish > Instituto de Doen?as Tropicais Natan Portella > Universidade Federal do Piau? > Rua Artur de Vasconcelos 151-Sul > 64001-450 Teresina-PI > Brazil > Telephones: +55 86 3222-4377 <+55%2086%203222-4377> (W), > +55 86 3221-3062 <+55%2086%203221-3062> (W), > +55 86 3237-1075 <+55%2086%203237-1075> (R). > > > Aviso: As informa??es contidas nesta mensagem s?o CONFIDENCIAIS, protegidas > pelo sigilo legal, por direitos autorais e destinadas exclusivamente ? > pessoa ou organiza??o para a qual a mensagem foi destinada. > Warning: This message is meant only for the intended recipient of the > transmission. It is forbidden any unauthorized use, alteration, > reproduction, and distribution. If you are not the correct recipient, > please notify us immediately by return e-mail and delete this message from > your system. > _______________________________________________ > Leish-l mailing list > Leish-l at lineu.icb.usp.br > http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l > > -- > This email was sent by icb.usp.br > -- Carlos H. N. Costa, MD, DSc. Chefe/Head Departamento de Medicina Comunit?ria Centro de Ci?ncias da Sa?de Coordenador/Coordinator Laborat?rio de Leishmanioses - LabLeish Instituto de Doen?as Tropicais Natan Portella Universidade Federal do Piau? Rua Artur de Vasconcelos 151-Sul 64001-450 Teresina-PI Brazil Telephones: +55 86 3222-4377 (W), +55 86 3221-3062 (W), +55 86 3237-1075 (R). Aviso: As informa??es contidas nesta mensagem s?o CONFIDENCIAIS, protegidas pelo sigilo legal, por direitos autorais e destinadas exclusivamente ? pessoa ou organiza??o para a qual a mensagem foi destinada. Warning: This message is meant only for the intended recipient of the transmission. It is forbidden any unauthorized use, alteration, reproduction, and distribution. If you are not the correct recipient, please notify us immediately by return e-mail and delete this message from your system. From fandrade47 at gmail.com Thu Jul 27 20:23:01 2017 From: fandrade47 at gmail.com (Fernando Andrade Narvaez) Date: Thu, 27 Jul 2017 18:23:01 -0500 Subject: [Leish-l] Fwd: infectivity or infectiousness? In-Reply-To: References: Message-ID: Dear Carlos, Please review the Biology Online Dictionary. I agree that is very important to have a clear idea of their meaning and knowledge to employ them accurately. *Infectivity*: is related to the causative infectious agent, in case of leishmaniacs we are talking of *Leishmania* spp. It is usually used to talk about the capability of *Leishmania* spp. to enter the macrophages and colonize them, therefore entering, surviving and multiplying in a susceptible host. Thus is used to talk of the ability of the pathogen (your number 3 option). *Infectiousness* "the state or quality of being infectious" derive from *Infectious* term used to refer an *infectious* disease (able to be passed from one person,anmal to other in vector borne disease through the insect vector), an *infectious* patient (able to pass a diseases of one person or animal to other), *infectious *period (used mainly for viral diseases). Thus infectiousness is used to talk about the host or sand fly ability to pass or transfer (options 1 & 2). Infectiosity is a synonym of infectiousness. Best regards, Dr. Fernando J. Andrade Narv?ez Laboratorio de Inmunolog?a Centro de Investigaciones Regionales "Dr. Hideyo Noguchi" Universidad Aut?noma de Yucat?n Ave. Itz?es No. 490x59 CP 97000 M?rida, Yucat?n, M?xico Tel.: 999 924-6412 ext. 1156 y 1155 Fax: 999 923-6120 E-mails: anarvaez at correo.uady.mx, fandrade47 at gmail.com Fernando J. Andrade-Narvaez, MD, PhD Immunology Laboratory Centro de Investigaciones Regionales "Dr. Hideyo Noguchi" Universidad Aut?noma de Yucat?n, Ave. Itz?es No. 490x59 CP 97000 M?rida, Yucat?n, M?xico Tel.: 52 999 924-6412 ext. 1156 & 1155 Fax: 52 999 923-6120 E-mails: anarvaez at correo.uady.mx, fandrade47 at gmail.com 2017-06-24 8:57 GMT-05:00 Carlos Costa : > sorry, the last one should be infectiosity (wiki). > > 2017-06-24 10:21 GMT-03:00 Carlos Costa : > > > Dear fellows, > > > > Are there any hints on where or when to use infectivity, infectiousness > or > > infectiousness? > > 1) Talking about the host ability to transmit a pathogen. > > 2) Talking about the sand fly ability to transmit a pathogen > > 3) Talking about the pathogen ability to be transmitted. > > > > Are they synonyms? Can we use any form in any situation? > > > > I looked at the web but could not find anything sharp about this. > > > > Cheers, > > > > Carlos. > > > > -- > > Carlos H. N. Costa, MD, DSc. > > Chefe/Head > > Departamento de Medicina Comunit?ria > > Centro de Ci?ncias da Sa?de > > Coordenador/Coordinator > > Laborat?rio de Leishmanioses - LabLeish > > Instituto de Doen?as Tropicais Natan Portella > > Universidade Federal do Piau? > > Rua Artur de Vasconcelos 151-Sul > > 64001-450 Teresina-PI > > Brazil > > Telephones: +55 86 3222-4377 <+55%2086%203222-4377> (W), > > +55 86 3221-3062 <+55%2086%203221-3062> (W), > > +55 86 3237-1075 <+55%2086%203237-1075> (R). > > > > > > Aviso: As informa??es contidas nesta mensagem s?o CONFIDENCIAIS, > > protegidas pelo sigilo legal, por direitos autorais e destinadas > > exclusivamente ? pessoa ou organiza??o para a qual a mensagem foi > destinada. > > Warning: This message is meant only for the intended recipient of the > > transmission. It is forbidden any unauthorized use, alteration, > > reproduction, and distribution. If you are not the correct recipient, > > please notify us immediately by return e-mail and delete this message > from > > your system. > > > > > > -- > Carlos H. N. Costa, MD, DSc. > Chefe/Head > Departamento de Medicina Comunit?ria > Centro de Ci?ncias da Sa?de > Coordenador/Coordinator > Laborat?rio de Leishmanioses - LabLeish > Instituto de Doen?as Tropicais Natan Portella > Universidade Federal do Piau? > Rua Artur de Vasconcelos 151-Sul > 64001-450 Teresina-PI > Brazil > Telephones: +55 86 3222-4377 (W), > +55 86 3221-3062 (W), > +55 86 3237-1075 (R). > > > Aviso: As informa??es contidas nesta mensagem s?o CONFIDENCIAIS, protegidas > pelo sigilo legal, por direitos autorais e destinadas exclusivamente ? > pessoa ou organiza??o para a qual a mensagem foi destinada. > Warning: This message is meant only for the intended recipient of the > transmission. It is forbidden any unauthorized use, alteration, > reproduction, and distribution. If you are not the correct recipient, > please notify us immediately by return e-mail and delete this message from > your system. > _______________________________________________ > Leish-l mailing list > Leish-l at lineu.icb.usp.br > http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l > > -- > This email was sent by icb.usp.br >