[Leish-l] Confirm hits to Leishmania spp. in whole blood of mammals in the US?

Carlos Costa chncosta at gmail.com
Fri Apr 3 11:07:50 BRT 2015


A very important and challenging study, indeed, Joaquina! Congratulations
to the Spaniards! Carlos.

2015-03-06 9:32 GMT-03:00 joaquina <joaquina at ugr.es>:

> Dear Katherine,
> Your approach is wrong. Leishmania DNA can be amplified by PCR and
> directly sequenced from host or vector biological samples. However, you
> must carefully choose a convenient PCR technique as not every of them give
> the same results. Also, you should take into account the presence of other
> trypanosomatid species apart from Leishmania, both in vector and in the
> reservoir and it is necessary to differentiate them properly. L. infantum
> is endemic in southwestern Europe but it is becoming clear that the classic
> epidemiological cycle dog-sandfly-human is turning into a network of animal
> species that collaborate with the dog in the maintenance of the parasite
> under natural conditions. Our research in southeastern Spain involved as
> reservoir, apart from the dog, the wild rabbit (Oryctolagus cuniculus)
> (please find attached the paper about it). Our studies also support the
> importance of rodents such as Rattus rattus, Apodemus sylvaticus and Mus
> musculus in the life cycle of L. infantum (article in revision in
> Parasitology Research)
> Joaquina Martin-Sanchez
> Dpto Parasitologia
> Fac Farmacia
> Granada University
> Granada, Spain
>
> El 2015-03-06 00:30, Sayler,Katherine A escribió:
>
>> Dear readers,
>>
>> Thank you sincerely for your time. I am a tick-borne disease biologist
>> by trade, so this is outside of my realm.
>>
>> I am trying to confirm some preliminary data that suggests the
>> _Leishmania_ spp. are circulating in limited geographic locales in
>> southern Florida. Is it at all reasonable to expect to detect the DNA
>> of the trypanosomatids in the blood of infected wildlife? My suspicion
>> is that this is completely improbable, as it appears that in order to
>> detect DNA in enough quantity or quality for sequencing, you need
>> cultured organisms. From sandflies it appears that it is possible to
>> PCR amplify and sequence directly from the vector, but never from the
>> reservoir (or potential reservoir) host. Is this true? Any advice or
>> insight would be greatly appreciated and I am tremendously naïve and
>> this is clearly not my area of expertise.
>>
>> Thank you again for reading my message,
>>
>> Katherine
>>
>> Katherine Sayler, M.Ed., Ph.D.
>> Postdoctoral Associate | Department of Wildlife Ecology and
>> Conservation
>> Molecular Ecology Laboratory | 2322 Mowry Rd. Gainesville, FL 32611
>> Phone: 352-871-3259
>>
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-- 
Carlos H. N. Costa, MD, DSc.
Coordenador
Laboratório de Leishmanioses - LabLeish
Universidade Federal do Piauí
Instituto de Doenças Tropicais Natan Portella
Rua Artur de Vasconcelos 151-Sul
64001-450 Teresina-PI
Brazil
Telephones: +55 86 3222-4377 (W),
+55 86 3221-3062 (W),
+55 86 3237-1075 (R).


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