From Hira.Nakhasi at fda.hhs.gov Mon Mar 11 10:13:35 2013
From: Hira.Nakhasi at fda.hhs.gov (Nakhasi, Hira)
Date: Mon, 11 Mar 2013 13:13:35 +0000
Subject: [Leish-l] hot spots
In-Reply-To: <20130210193235.78pdjqsgd9j48kc8@webmailum1.univ-montp1.fr>
References: <CAJsAp4MReYD4uA8q72ULp=HBDGB9WW0mu6rzRJYRE0fga0eC9A@mail.gmail.com>
<20130210193235.78pdjqsgd9j48kc8@webmailum1.univ-montp1.fr>
Message-ID: <C1C710814C0DA84A9B22DCCBCA8E890E1B9138C9@FDSWP3315.fda.gov>
Carlos,
New Approaches to Vaccine development for Leishmaniasis.
Hira
-----Original Message-----
From: leish-l-bounces at lineu.icb.usp.br [mailto:leish-l-bounces at lineu.icb.usp.br] On Behalf Of Patrick Bastien
Sent: Sunday, February 10, 2013 1:33 PM
To: leish-l at lineu.icb.usp.br
Subject: Re: [Leish-l] hot spots
One example : The existence, modalities and importance of sexuality
(pr parasexuality).
Cordialement
Professeur Patrick Bastien
Director, Laboratoire de Parasitologie-Mycologie, Faculte de M?decine
Centre National de Reference des Leishmanioses
Deputy Director, UMR MIVEGEC (CNRS 5290 - IRD 224 - Universites Montpellier 1
et 2)
CHU de Montpellier
39 Avenue Charles Flahault
34295 Montpellier Cedex 5, France
----- Message de chncosta at gmail.com ---------
Date?: Thu, 7 Feb 2013 16:38:58 -0300
De?: Carlos Costa <chncosta at gmail.com>
R?pondre ??: Carlos Costa <chncosta at gmail.com>
Objet?: [Leish-l] hot spots
??: Leish-L <Leish-l at lineu.icb.usp.br>
> What are the most polemic issues in leishmaniasis?
>
> --
> Carlos H. N. Costa, MD, DSc.
> Sociedade Brasileira de Medicina Tropical
> (Brazilian Society of Tropical Medicine)
> President
>
> Universidade Federal do Piau?
> Instituto de Doen?as Tropicais Natan Portella
> Rua Artur de Vasconcelos 151-Sul
> 64001-450 Teresina-PI
> Brazil
> Telephones: +55 86 3222-4377 (W),
> +55 86 3221-3062 (W),
> +55 86 3237-1075 (R).
>
>
> Aviso: As informa??es contidas nesta mensagem s?o CONFIDENCIAIS, protegidas
> pelo sigilo legal, por direitos autorais e destinadas exclusivamente ?
> pessoa ou organiza??o para a qual a mensagem foi destinada.
> Warning: This message is meant only for the intended recipient of the
> transmission. It is forbidden any unauthorized use, alteration,
> reproduction and distribution. If you are not the correct recipient, please
> notify us immediately by return e-mail and delete this message from your
> system.
>
----- Fin du message de chncosta at gmail.com -----
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From H.Schallig at kit.nl Mon Mar 11 09:38:07 2013
From: H.Schallig at kit.nl (Schallig, Henk)
Date: Mon, 11 Mar 2013 13:38:07 +0100
Subject: [Leish-l] hot spots
In-Reply-To: <817688560.61581.1360757685025.JavaMail.root@hermes.ufpe.br>
References: <24C925DA-F1D1-463B-A393-B9083FAD5274@uniba.it>
<817688560.61581.1360757685025.JavaMail.root@hermes.ufpe.br>
Message-ID: <8BBA49AFE63E5741AEAFB2D2362EFE040152406DC0DA@MBXCCR1.kit.nl>
Dear All,
As our institute is producing the freeze-dried DAT antigen, I am most willing to participate in syuch a round table.
Kind regards,
Henk D.F.H. Schallig, PhD
Research coordinator Parasitology
...............................................................................
KIT Biomedical Research
T +31 (0)20 5665447
Royal Tropical Institute | We think. We share. We act.
Meibergdreef 39
1105 AZ Amsterdam
The Netherlands
F +31 (0)20 6971841
www.kit.nl<http://www.kit.nl/>
New! Visit KIT's Leptospira Library<http://www.kit.nl/Leptospira-Library>
From: leish-l-bounces at lineu.icb.usp.br [mailto:leish-l-bounces at lineu.icb.usp.br] On Behalf Of Paulo Paes de Andrade
Sent: woensdag 13 februari 2013 13:15
To: Filipe Dantas Torres
Cc: Leish-L
Subject: Re: [Leish-l] hot spots
Dear Colleagues.
In spite of all recent development on diagnosis, I think human serodiagnosis in hospitals is still a challenge. DAT was suggested by WHO as the test of choice because of its high sensitivity and specificity, and possibly also because of its low costs. But it has some drawbacks. It would be very interesting to have Abdallah El Harith (DAT) and other scientists dedicated to this question participating in a round table focused on the practical use of their tests for human diagnosis in hospitals.
Paulo Andrade
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From CCassetti at niaid.nih.gov Mon Mar 11 10:26:26 2013
From: CCassetti at niaid.nih.gov (Cassetti, Cristina (NIH/NIAID) [E])
Date: Mon, 11 Mar 2013 13:26:26 +0000
Subject: [Leish-l] What are the most polemic issues in leishmaniasis?
In-Reply-To: <5EA2E08A390CC64989B542B01E4F31F30709FCF5@ExchMB1.DNDI-ORG.LOCAL>
References: <5EA2E08A390CC64989B542B01E4F31F30709FCF5@ExchMB1.DNDI-ORG.LOCAL>
Message-ID: <39A8E68A7613ED4E8DCE3AC85F58BE5F4F34AE@MLBXV01.nih.gov>
Can you please take me off this mailing list ?
Thank you,
Cristina
From: Farrokh Modabber [mailto:fmodabber at dndi.org]
Sent: Monday, February 11, 2013 7:38 PM
To: 'Carlos Costa'; 'Leish-L'
Subject: [Leish-l] What are the most polemic issues in leishmaniasis?
Dear Carlos,
I think Leishmania classification should be updated. I have problem with identification of Leishmania isolates based on non-biological markers. The ones used, i.e. genetic markers (not associated with known biological activity), iso-enzymes electrophoresis (all the isoenzymes have the same biological activity and changes somewhere in the enzyme molecule cause different electrophoretic migration). Now that we know conjugation (transfer of genetic material from one to another Leishmania) occur, and with the whole genome sequencing available, isn't it time to look for biological markers? The tools used, have helped us establish the evolutionary trees but do not help us in knowing what kind of disease each isolate will produce, their vector specificity, etc. I realize that disease outcome also depends on host response, but still some better markers may be identified.
What do you think?
All the best,
Farrokh
From: leish-l-bounces at lineu.icb.usp.br<mailto:leish-l-bounces at lineu.icb.usp.br> [mailto:leish-l-bounces at lineu.icb.usp.br] On Behalf Of Israel Cruz Mata
Sent: lundi 11 f?vrier 2013 09:46
To: 'Carlos Costa'; 'Leish-L'
Subject: Re: [Leish-l] hot spots
Hi Carlos,
I?m curious about these:
- "Leishmania siamensis": causing dermal and visceral human leishmaniasis in Thailand, cutaneous equine and bovine leishmaniasis in central Europe, and equine cutaneous leishmanaisis in USA.
Is it really the same parasite in all cases, it is a new Leishmania specie emerging worldwide?
- Leishmania in Macropods and its transmission by midges (Ceratopogonidae), not sandflies.
- Control strategies for ZVL in South America: alternatives for dog culling.
All the best,
Isra
De: leish-l-bounces at lineu.icb.usp.br<mailto:leish-l-bounces at lineu.icb.usp.br> [mailto:leish-l-bounces at lineu.icb.usp.br] En nombre de Carlos Costa
Enviado el: jueves, 07 de febrero de 2013 20:39
Para: Leish-L
Asunto: [Leish-l] hot spots
What are the most polemic issues in leishmaniasis?
--
Carlos H. N. Costa, MD, DSc.
Sociedade Brasileira de Medicina Tropical
(Brazilian Society of Tropical Medicine)
President
Universidade Federal do Piau?
Instituto de Doen?as Tropicais Natan Portella
Rua Artur de Vasconcelos 151-Sul
64001-450 Teresina-PI
Brazil
Telephones: +55 86 3222-4377 (W),
+55 86 3221-3062 (W),
+55 86 3237-1075 (R).
Aviso: As informa??es contidas nesta mensagem s?o CONFIDENCIAIS, protegidas pelo sigilo legal, por direitos autorais e destinadas exclusivamente ? pessoa ou organiza??o para a qual a mensagem foi destinada.
Warning: This message is meant only for the intended recipient of the transmission. It is forbidden any unauthorized use, alteration, reproduction and distribution. If you are not the correct recipient, please notify us immediately by return e-mail and delete this message from your system.
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From mallorie.hide at ird.fr Mon Mar 11 09:33:59 2013
From: mallorie.hide at ird.fr (Mallorie Hide)
Date: Mon, 11 Mar 2013 13:33:59 +0100
Subject: [Leish-l] hot spots
In-Reply-To: <20130311122755.0C795575474@fx806.security-mail.net>
Message-ID: <20130311123421.57563AAC305@fx405.security-mail.net>
Dear all,
Concerning,
1) Asymptomatic Leishmaniasis
Please find in attach a manuscript in press that I hope some of you will find of interest ("Parasitic genotypes appear to differ in leishmaniasis patients compared with
asymptomatic related carriers ?).
Best regards,
Mallorie
*********************
Dr Mallorie HIDE, Ph.D
cid:image002.jpg at 01CC7C61.E9921A40
MIVEGEC (CNRS 5290-IRD 224-UM1-UM2) (ex-GEMI UR165/UMR2724)
Equipe Genesys (G?n?tique et Biodiversit? des Syst?mes Infectieux)
911 av Agropolis BP 64501, 34394 MONTPELLIER Cedex 5, FRANCE
Tel : (33)4.67.41.63.91
Fax : (33)4.67.41.62.99
http://www.mivegec.ird.fr/
*********************
De : leish-l-bounces at lineu.icb.usp.br [mailto:leish-l-bounces at lineu.icb.usp.br] De la part de Francisco Pasquau
Envoy? : jeudi 14 f?vrier 2013 20:40
? : 'Carlos Costa'; 'Leish-L'
Objet : Re: [Leish-l] hot spots
2) Asymptomatic Leishmaniasis
3) Secondary Prophylaxis in immunosuppressed patient with Visceral Leishmaniasis (HIV, Transplant, anti TNF therapy...)
4) "Active chronic visceral Leishmaniasis"
F.Pasquau Lia?o
Hospital Marina Baixa
Alicante. Spain
_____
De: leish-l-bounces at lineu.icb.usp.br [mailto:leish-l-bounces at lineu.icb.usp.br] En nombre de Carlos Costa
Enviado el: jueves, 07 de febrero de 2013 8:39
Para: Leish-L
Asunto: [Leish-l] hot spots
What are the most polemic issues in leishmaniasis?
--
Carlos H. N. Costa, MD, DSc.
Sociedade Brasileira de Medicina Tropical
(Brazilian Society of Tropical Medicine)
President
Universidade Federal do Piau?
Instituto de Doen?as Tropicais Natan Portella
Rua Artur de Vasconcelos 151-Sul
64001-450 Teresina-PI
Brazil
Telephones: +55 86 3222-4377 (W),
+55 86 3221-3062 (W),
+55 86 3237-1075 (R).
Aviso: As informa??es contidas nesta mensagem s?o CONFIDENCIAIS, protegidas pelo sigilo legal, por direitos autorais e destinadas exclusivamente ? pessoa ou organiza??o para a qual a mensagem foi destinada.
Warning: This message is meant only for the intended recipient of the transmission. It is forbidden any unauthorized use, alteration, reproduction and distribution. If you are not the correct recipient, please notify us immediately by return e-mail and delete this message from your system.
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From OdeteAfonso at ihmt.unl.pt Mon Mar 11 11:19:58 2013
From: OdeteAfonso at ihmt.unl.pt (Odete Afonso)
Date: Mon, 11 Mar 2013 14:19:58 +0000
Subject: [Leish-l] hot spots
In-Reply-To: <20130210193235.78pdjqsgd9j48kc8@webmailum1.univ-montp1.fr>
References: <CAJsAp4MReYD4uA8q72ULp=HBDGB9WW0mu6rzRJYRE0fga0eC9A@mail.gmail.com>
<20130210193235.78pdjqsgd9j48kc8@webmailum1.univ-montp1.fr>
Message-ID: <33C84C69C522A449B64A9E007F1D5832C5503A81@EMS01.ihmt.unl.pt>
Dear Colleagues
Tourists with pets: to visit countries with leishmaniases active foci - how to know the potential transmission risks?
Best regards,
Odete
M. Odete Afonso, MD, PhD
Professor of Medical Entomology
Instituto de Higiene e Medicina Tropical,
Universidade Nova de Lisboa
Lisbon, Portugal
-----Mensagem original-----
De: leish-l-bounces at lineu.icb.usp.br [mailto:leish-l-bounces at lineu.icb.usp.br] Em nome de Patrick Bastien
Enviada: domingo, 10 de Fevereiro de 2013 18:33
Para: leish-l at lineu.icb.usp.br
Assunto: Re: [Leish-l] hot spots
One example : The existence, modalities and importance of sexuality
(pr parasexuality).
Cordialement
Professeur Patrick Bastien
Director, Laboratoire de Parasitologie-Mycologie, Faculte de M?decine
Centre National de Reference des Leishmanioses
Deputy Director, UMR MIVEGEC (CNRS 5290 - IRD 224 - Universites Montpellier 1
et 2)
CHU de Montpellier
39 Avenue Charles Flahault
34295 Montpellier Cedex 5, France
----- Message de chncosta at gmail.com ---------
Date?: Thu, 7 Feb 2013 16:38:58 -0300
De?: Carlos Costa <chncosta at gmail.com>
R?pondre ??: Carlos Costa <chncosta at gmail.com>
Objet?: [Leish-l] hot spots
??: Leish-L <Leish-l at lineu.icb.usp.br>
> What are the most polemic issues in leishmaniasis?
>
> --
> Carlos H. N. Costa, MD, DSc.
> Sociedade Brasileira de Medicina Tropical
> (Brazilian Society of Tropical Medicine)
> President
>
> Universidade Federal do Piau?
> Instituto de Doen?as Tropicais Natan Portella
> Rua Artur de Vasconcelos 151-Sul
> 64001-450 Teresina-PI
> Brazil
> Telephones: +55 86 3222-4377 (W),
> +55 86 3221-3062 (W),
> +55 86 3237-1075 (R).
>
>
> Aviso: As informa??es contidas nesta mensagem s?o CONFIDENCIAIS, protegidas
> pelo sigilo legal, por direitos autorais e destinadas exclusivamente ?
> pessoa ou organiza??o para a qual a mensagem foi destinada.
> Warning: This message is meant only for the intended recipient of the
> transmission. It is forbidden any unauthorized use, alteration,
> reproduction and distribution. If you are not the correct recipient, please
> notify us immediately by return e-mail and delete this message from your
> system.
>
----- Fin du message de chncosta at gmail.com -----
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From chrisb at ClarkCountyNV.gov Mon Mar 11 10:02:57 2013
From: chrisb at ClarkCountyNV.gov (Chris Bramley)
Date: Mon, 11 Mar 2013 13:02:57 +0000
Subject: [Leish-l] Leish-l Digest, Vol 60, Issue 6
In-Reply-To: <CADnthVRv5-M_iDZDZA1oGj2OSw1VE=4mCvXg=u=u9FKy0L8ipg@mail.gmail.com>
Message-ID: <7D9DC5213A95B343B5B3E0E27D6491D4022B262D@CCEXMBX03M.co.clark.nv.us>
Please remove me from your e-mail list.
Christopher Bramley
Public Works Vector Control & News Rack Enforcement Supervisor
5809 E. Flamingo Rd.
LV, NV. 89122
Office - 702-455-7543
Fax -702-898-2874
Cell -702-281-1262
From: Gustavo Benaim [mailto:gbenaim at gmail.com]
Sent: Sunday, January 27, 2013 08:17 AM Pacific Standard Time
To: leish-l at lineu.icb.usp.br <leish-l at lineu.icb.usp.br>
Subject: Re: [Leish-l] Leish-l Digest, Vol 60, Issue 6
Dear Colleagues
I am attaching a recent published manuspcrit that I think some of you will find of interest
It concerns the description o f a new plama membrane calcium channel in Lesihmania mexicana, which is activated by the sphingolipid Sphingosine.
Best regards
Gustavo Benaim
Dr. Gustavo Benaim
Laboratorio de Se?alizaci?n Celular y Bioqu?mica de Par?sitos
Instituto de Estudios Avanzados (IDEA)
Carretera Nacional Hoyo de la Puerta
Sartenejas. Baruta.
Caracas, Venezuela.
Phone: 58-212-903-5120
FAX: 58-212-903-5118
e mail: gbenaim at idea.gob.ve<mailto:gbenaim at idea.gob.ve>
&
Instituto de Biolog?a Experimental
Facultad de Ciencias
Universidad Central de Venezuela
Caracas, Venezuela.
e mail: gbenaim at reacciun.ve<mailto:gbenaim at reacciun.ve>
2013/1/27 <leish-l-request at lineu.icb.usp.br<mailto:leish-l-request at lineu.icb.usp.br>>
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Today's Topics:
1. Rich Titus (Marcelo Ramalho-Ortigao)
----------------------------------------------------------------------
Message: 1
Date: Thu, 24 Jan 2013 14:14:53 -0500 (EST)
From: "Marcelo Ramalho-Ortigao" <mortigao at k-state.edu<mailto:mortigao at k-state.edu>>
Subject: [Leish-l] Rich Titus
To: "'jeffrey shaw'" <jayusp at hotmail.com<mailto:jayusp at hotmail.com>>, <leish-l at lineu.icb.usp.br<mailto:leish-l at lineu.icb.usp.br>>
Message-ID:
<5f027e62.00001484.00000012 at ENT-ORTIGEEBC.ksre-ad.oznet.ksu.edu<mailto:5f027e62.00001484.00000012 at ENT-ORTIGEEBC.ksre-ad.oznet.ksu.edu>>
Content-Type: text/plain; charset="us-ascii"
Dear Jeff - I have some said news to report to the Leish-sand fly
community
Yesterday I learned about Richard Titus passing in Fort Collins, Colorado,
this past Sunday January 20th. Richard was an estimated colleague whom I
had the pleasure of meeting many years ago and have maintained close
contact in the last few years. Richard had a significant impact in the
field of sand flies and Leishmania, and many folks will remember him from
his contributions regarding the role of sand fly saliva and salivary
molecules in exacerbation of disease and immunomodulation in vertebrates.
>From the seminal work Richard and others published, many of us have
embarked in understanding the roles of saliva and salivary proteins not
only with regard to transmission of Leishmania by sand flies, but also in
other vector-pathogen models. Richard also significantly contributed to
the training of many students from all over the globe who spent time in
his lab at Colorado State University in Fort Collins. I recall one of his
stories about his nearly 25 years of working with vector saliva and having
to explain to family members what was that he did as an MD. Richard would
simply state that "after all the hard work of going through med school and
hours and hours in the lab he worked with spit".
He was a good colleague and someone I will miss and remember dearly.
Richard is survived by his daughter Katherine and grandson Christopher. I
also was informed that funeral arrangements are being or will be made
locally by Bohlander Funeral Chapel (www.bohlenderfuneralchapel.com<http://www.bohlenderfuneralchapel.com>).
Regards,
Marcelo
Marcelo Ramalho-Ortigao
Associate Professor
Department of Entomology
Kansas State University
106 Waters Hall Annex
Manhattan, KS 66506-4004
Tel: (785) 532-0139<tel:%28785%29%20532-0139>
Fax: (785) 532-6232<tel:%28785%29%20532-6232>
email: mortigao at ksu.edu<mailto:mortigao at ksu.edu>
------------------------------
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Leish-l at lineu.icb.usp.br<mailto:Leish-l at lineu.icb.usp.br>
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End of Leish-l Digest, Vol 60, Issue 6
**************************************
--
Dr. Gustavo Benaim
Laboratorio de Se?alizaci?n Celular y Bioqu?mica de Par?sitos.
Instituto de Estudios Avanzados (IDEA)
Caracas, Venezuela
Telf: 58-212-903-5120
FAX: 58-212-903-5118
email: gbenaim at idea.gob.ve<mailto:gbenaim at idea.gob.ve>
&
Instituto de Biolog?a Experimetal
Facultad de Ciencias
Universidad Central de Venezuela
Caracas, Venezuela
Telf 58-212-751-0111
FAX: 58-212-753-5897
e mail: gbenaim at reacciun.ve
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From clos at bni-hamburg.de Mon Mar 11 11:46:07 2013
From: clos at bni-hamburg.de (Clos Joachim)
Date: Mon, 11 Mar 2013 15:46:07 +0100
Subject: [Leish-l] Fwd: hot spots
References: <FE318E54-2932-4C1A-9BAB-D93A0C17F806@bni-hamburg.de>
Message-ID: <89734F2D-D0BF-4B64-ABC4-BFD4BF842160@bni-hamburg.de>
> Are axenic amastigotes a valid model (and for what?), the first developmental step towards real amastigotes, or just stressed-out promastigotes? Many of us use axenics and swear by them, others are skeptical, others will accept what's inside a macrophage, still others only want amastigotes isolated from something with a fur. I believe we should come to some sort of common language and interpretation. I am somewhere in the middle myself, preferring to do things in macrophages whenever possible.
> Cheers!
>
>
> Am 07.02.2013 um 20:38 schrieb Carlos Costa:
>
>> What are the most polemic issues in leishmaniasis?
>>
>> --
>> Carlos H. N. Costa, MD, DSc.
>> Sociedade Brasileira de Medicina Tropical
>> (Brazilian Society of Tropical Medicine)
>> President
>>
>> Universidade Federal do Piau?
>> Instituto de Doen?as Tropicais Natan Portella
>> Rua Artur de Vasconcelos 151-Sul
>> 64001-450 Teresina-PI
>> Brazil
>> Telephones: +55 86 3222-4377 (W), +55 86 3221-3062 (W), +55 86 3237-1075 (R).
>>
>>
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>> _______________________________________________
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>
>
>
> Joachim Clos, Dr. rer. nat.
> Senior Staff Scientist
> Group Leader
> Bernhard Nocht Institute for Tropical Medicine
> Bernhard Nocht St. 74, D-20359 Hamburg, FRG
> Phone +49 40 42818-481; Fax -512
> Skype: joachim.clos
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>
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From isabelle.thiery at pasteur.fr Mon Mar 11 11:58:16 2013
From: isabelle.thiery at pasteur.fr (Isabelle Thiery)
Date: Mon, 11 Mar 2013 15:58:16 +0100
Subject: [Leish-l] What are the most polemic issues in leishmaniasis?
In-Reply-To: <39A8E68A7613ED4E8DCE3AC85F58BE5F4F34AE@MLBXV01.nih.gov>
References: <5EA2E08A390CC64989B542B01E4F31F30709FCF5@ExchMB1.DNDI-ORG.LOCAL>
<39A8E68A7613ED4E8DCE3AC85F58BE5F4F34AE@MLBXV01.nih.gov>
Message-ID: <513DF108.2070305@pasteur.fr>
Can you please take me off this mailing list ? thanks
Isabelle
Le 11/03/13 14:26, Cassetti, Cristina (NIH/NIAID) [E] a ?crit :
>
> Can you please take me off this mailing list ?
>
> Thank you,
>
> Cristina
>
> *From:*Farrokh Modabber [mailto:fmodabber at dndi.org]
> *Sent:* Monday, February 11, 2013 7:38 PM
> *To:* 'Carlos Costa'; 'Leish-L'
> *Subject:* [Leish-l] What are the most polemic issues in leishmaniasis?
>
> Dear Carlos,
>
> I think /Leishmania/ classification should be updated. I have problem
> with identification of /Leishmania/ isolates based on non-biological
> markers. The ones used, i.e. genetic markers (not associated with
> known biological activity), iso-enzymes electrophoresis (all the
> isoenzymes have the same biological activity and changes somewhere in
> the enzyme molecule cause different electrophoretic migration). Now
> that we know conjugation (transfer of genetic material from one to
> another /Leishmania/) occur, and with the whole genome sequencing
> available, isn't it time to look for biological markers? The tools
> used, have helped us establish the evolutionary trees but do not help
> us in knowing what kind of disease each isolate will produce, their
> vector specificity, etc. I realize that disease outcome also depends
> on host response, but still some better markers may be identified.
>
> What do you think?
>
> All the best,
>
> Farrokh
>
> *From:*leish-l-bounces at lineu.icb.usp.br
> <mailto:leish-l-bounces at lineu.icb.usp.br>
> [mailto:leish-l-bounces at lineu.icb.usp.br] *On Behalf Of *Israel Cruz Mata
> *Sent:* lundi 11 f?vrier 2013 09:46
> *To:* 'Carlos Costa'; 'Leish-L'
> *Subject:* Re: [Leish-l] hot spots
>
> Hi Carlos,
>
> I?m curious about these:
>
> -"Leishmania siamensis": causing dermal and visceral human
> leishmaniasis in Thailand, cutaneous equine and bovine leishmaniasis
> in central Europe, and equine cutaneous leishmanaisis in USA.
>
> Is it really the same parasite in all cases, it is a new Leishmania
> specie emerging worldwide?
>
> -Leishmania in Macropods and its transmission by midges
> (Ceratopogonidae), not sandflies.
>
> -Control strategies for ZVL in South America: alternatives for dog
> culling.
>
> All the best,
>
> Isra
>
> *De:*leish-l-bounces at lineu.icb.usp.br
> <mailto:leish-l-bounces at lineu.icb.usp.br>
> [mailto:leish-l-bounces at lineu.icb.usp.br] *En nombre de *Carlos Costa
> *Enviado el:* jueves, 07 de febrero de 2013 20:39
> *Para:* Leish-L
> *Asunto:* [Leish-l] hot spots
>
> What are the most polemic issues in leishmaniasis?
>
> --
>
> Carlos H. N. Costa, MD, DSc.
>
> Sociedade Brasileira de Medicina Tropical
>
> (Brazilian Society of Tropical Medicine)
>
> President
>
> Universidade Federal do Piau?
>
> Instituto de Doen?as Tropicais Natan Portella
>
> Rua Artur de Vasconcelos 151-Sul
>
> 64001-450 Teresina-PI
>
> Brazil
>
> Telephones: +55 86 3222-4377 (W),
>
> +55 86 3221-3062 (W),
>
> +55 86 3237-1075 (R).
>
> Aviso: As informa??es contidas nesta mensagem s?o CONFIDENCIAIS,
> protegidas pelo sigilo legal, por direitos autorais e destinadas
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> transmission. It is forbidden any unauthorized use, alteration,
> reproduction and distribution. If you are not the correct recipient,
> please notify us immediately by return e-mail and delete this message
> from your system.
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> ************************* AVISO LEGAL *************************
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> _______________________________________________
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> http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l
>
> --
> This email was sent by icb.usp.br
--
Isabelle Thiery, PhD
Charg?e de mission pour la logistique
Batiment 7 Le Pasteur, RDC droit, pi?ce 16
TEl : 01 45 68 83 43
Isabelle.thiery at pasteur.fr
Institut Pasteur, 25 rue du Docteur Roux
75724 Paris Cedex 15, France
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From jayusp at hotmail.com Mon Mar 11 18:47:19 2013
From: jayusp at hotmail.com (jeffrey shaw)
Date: Mon, 11 Mar 2013 21:47:19 +0000
Subject: [Leish-l] Please read this message - Leish-L Administration
Message-ID: <DUB124-W194FC6A5DCD9B2EF5A8843CBE10@phx.gbl>
Periodically
subscribers send emails to Leish-L of a mixed personal and
professional nature ranging from congratulations to condolences. As the
number of subscribers increases so do these messages. Given the quantity
of emails that all of us receive some of you have understandingly found this a
little irritating.
In an effort
to tackle this problem such messages will be gathered together and sent as one.
So if your
message is in the above category and does not appear immediately please be patient!
Also emails
that are replies to an individual are forwarded to that person and are not circulated.
Please be careful when using the email
reply option to a Leish-L message. Some request administrative help by simply
replying to a Leish-L message that has nothing to do with their request. Such
messages are difficult to detect and result in them being inadvertently
circulated and the problem is not resolved!
Finally if you send an email to Leish-L
and then decide that you do not want it circulated please send me an email (jayusp at hotmail.com) or another one to
Leish-L as quickly as possible CLEARLY asking me to delete it. The time between
receiving and authorization is normally between 24 - 72 hours
If you have any questions or comments
about the above please do not hesitate in contacting me.
With best wishes
Jeffrey Shaw
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From diemen at coma.es Mon Mar 11 13:29:12 2013
From: diemen at coma.es (=?iso-8859-1?Q?Diego_Torr=FAs_Tendero?=)
Date: Mon, 11 Mar 2013 16:29:12 -0000
Subject: [Leish-l] hot spots
In-Reply-To: <5FE4BF79A2094764AF75B5376AB4ADE4@PC01>
References: <CAJsAp4MReYD4uA8q72ULp=HBDGB9WW0mu6rzRJYRE0fga0eC9A@mail.gmail.com>
<5FE4BF79A2094764AF75B5376AB4ADE4@PC01>
Message-ID: <6EEAB2E4BA3C4116872FB719DF59CCED@usuarioc157d77>
I agree with my colleague and friend Dr. Pasquau
Diego Torr?s Tendero
Hospital General Universitario de Alicante
Alicante. Spain
_____
De: leish-l-bounces at lineu.icb.usp.br
[mailto:leish-l-bounces at lineu.icb.usp.br] En nombre de Francisco Pasquau
Enviado el: jueves, 14 de febrero de 2013 20:40
Para: 'Carlos Costa'; 'Leish-L'
Asunto: Re: [Leish-l] hot spots
1) Asymptomatic Leishmaniasis
2) Secondary Prophylaxis in immunosuppressed patient with Visceral
Leishmaniasis (HIV, Transplant, anti TNF therapy
)
3) ?Active chronic visceral Leishmaniasis?
F.Pasquau Lia?o
Hospital Marina Baixa
Alicante. Spain
_____
De: leish-l-bounces at lineu.icb.usp.br
[mailto:leish-l-bounces at lineu.icb.usp.br] En nombre de Carlos Costa
Enviado el: jueves, 07 de febrero de 2013 8:39
Para: Leish-L
Asunto: [Leish-l] hot spots
What are the most polemic issues in leishmaniasis?
--
Carlos H. N. Costa, MD, DSc.
Sociedade Brasileira de Medicina Tropical
(Brazilian Society of Tropical Medicine)
President
Universidade Federal do Piau?
Instituto de Doen?as Tropicais Natan Portella
Rua Artur de Vasconcelos 151-Sul
64001-450 Teresina-PI
Brazil
Telephones: +55 86 3222-4377 (W),
+55 86 3221-3062 (W),
+55 86 3237-1075 (R).
Aviso: As informa??es contidas nesta mensagem s?o CONFIDENCIAIS, protegidas
pelo sigilo legal, por direitos autorais e destinadas exclusivamente ?
pessoa ou organiza??o para a qual a mensagem foi destinada.
Warning: This message is meant only for the intended recipient of the
transmission. It is forbidden any unauthorized use, alteration,
reproduction and distribution. If you are not the correct recipient, please
notify us immediately by return e-mail and delete this message from your
system.
----------------------------------------------------------------------------
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Este mensaje NO ha sido clasificado como SPAM. Si se trata de un mensaje de
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From vlfcn at hotmail.com Mon Mar 11 12:47:19 2013
From: vlfcn at hotmail.com (Vera Camargo-Neves)
Date: Mon, 11 Mar 2013 15:47:19 -0000
Subject: [Leish-l] hot spots
In-Reply-To: <33C84C69C522A449B64A9E007F1D5832C5503A81@EMS01.ihmt.unl.pt>
References: <CAJsAp4MReYD4uA8q72ULp=HBDGB9WW0mu6rzRJYRE0fga0eC9A@mail.gmail.com>,
<20130210193235.78pdjqsgd9j48kc8@webmailum1.univ-montp1.fr>,
<33C84C69C522A449B64A9E007F1D5832C5503A81@EMS01.ihmt.unl.pt>
Message-ID: <BLU172-W20B7D9D62983F17FA5700FBEE10@phx.gbl>
Excellent topic for discussion!I agree with the proposal of Odete's hot spot.We need to start discussing the issue on the movement of animals and the risk of Visceral Leishmaniasis and the inverse of introducing the parasite infection into free regions or the introduction of new strains with changes in the profile of resistance to drugs now used to treat beings human regions, endemic countries.Best Regards,
Vera L Fonseca de Camargo-Neves, PhDPesquisador Cient?fico VI (Medical Research Senior)Grupo de Estudos em Leishmanioses, Departamento de Epidemiologia e Orienta??o T?cnica (Study Group on Leishmaniasis, Department of Epidemiology and Technical Guidance)Superintend?ncia de Controle de Endemias (Superintendence of Control of Endemic Diseases)
Adress:Rua Paula Sousa, 166 1? andar Bairro da Luz, S?o Paulo, SP, BrasilCEP: 01027-000Tel: (55 11) 3311-1142Skype: veracamargoneves
> From: OdeteAfonso at ihmt.unl.pt
> To: patrick.bastien at univ-montp1.fr; leish-l at lineu.icb.usp.br
> Date: Mon, 11 Mar 2013 14:19:58 +0000
> CC: OdeteAfonso at ihmt.unl.pt
> Subject: Re: [Leish-l] hot spots
>
> Dear Colleagues
>
>
> Tourists with pets: to visit countries with leishmaniases active foci - how to know the potential transmission risks?
>
>
> Best regards,
>
> Odete
>
> M. Odete Afonso, MD, PhD
> Professor of Medical Entomology
> Instituto de Higiene e Medicina Tropical,
> Universidade Nova de Lisboa
> Lisbon, Portugal
>
>
>
> -----Mensagem original-----
> De: leish-l-bounces at lineu.icb.usp.br [mailto:leish-l-bounces at lineu.icb.usp.br] Em nome de Patrick Bastien
> Enviada: domingo, 10 de Fevereiro de 2013 18:33
> Para: leish-l at lineu.icb.usp.br
> Assunto: Re: [Leish-l] hot spots
>
> One example : The existence, modalities and importance of sexuality
> (pr parasexuality).
> Cordialement
>
> Professeur Patrick Bastien
> Director, Laboratoire de Parasitologie-Mycologie, Faculte de M?decine
> Centre National de Reference des Leishmanioses
> Deputy Director, UMR MIVEGEC (CNRS 5290 - IRD 224 - Universites Montpellier 1
> et 2)
> CHU de Montpellier
> 39 Avenue Charles Flahault
> 34295 Montpellier Cedex 5, France
>
> ----- Message de chncosta at gmail.com ---------
> Date : Thu, 7 Feb 2013 16:38:58 -0300
> De : Carlos Costa <chncosta at gmail.com>
> R?pondre ? : Carlos Costa <chncosta at gmail.com>
> Objet : [Leish-l] hot spots
> ? : Leish-L <Leish-l at lineu.icb.usp.br>
>
>
> > What are the most polemic issues in leishmaniasis?
> >
> > --
> > Carlos H. N. Costa, MD, DSc.
> > Sociedade Brasileira de Medicina Tropical
> > (Brazilian Society of Tropical Medicine)
> > President
> >
> > Universidade Federal do Piau?
> > Instituto de Doen?as Tropicais Natan Portella
> > Rua Artur de Vasconcelos 151-Sul
> > 64001-450 Teresina-PI
> > Brazil
> > Telephones: +55 86 3222-4377 (W),
> > +55 86 3221-3062 (W),
> > +55 86 3237-1075 (R).
> >
> >
> > Aviso: As informa??es contidas nesta mensagem s?o CONFIDENCIAIS, protegidas
> > pelo sigilo legal, por direitos autorais e destinadas exclusivamente ?
> > pessoa ou organiza??o para a qual a mensagem foi destinada.
> > Warning: This message is meant only for the intended recipient of the
> > transmission. It is forbidden any unauthorized use, alteration,
> > reproduction and distribution. If you are not the correct recipient, please
> > notify us immediately by return e-mail and delete this message from your
> > system.
> >
>
>
> ----- Fin du message de chncosta at gmail.com -----
>
>
>
> _______________________________________________
> Leish-l mailing list
> Leish-l at lineu.icb.usp.br
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>
> This email was sent by icb.usp.br
> _______________________________________________
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From skarora_in at yahoo.com Mon Mar 11 12:42:53 2013
From: skarora_in at yahoo.com (Sunil Arora)
Date: Mon, 11 Mar 2013 15:42:53 -0000
Subject: [Leish-l] Fwd: hot spots
In-Reply-To: <89734F2D-D0BF-4B64-ABC4-BFD4BF842160@bni-hamburg.de>
References: <FE318E54-2932-4C1A-9BAB-D93A0C17F806@bni-hamburg.de>
<89734F2D-D0BF-4B64-ABC4-BFD4BF842160@bni-hamburg.de>
Message-ID: <1363016524.46325.YahooMailNeo@web126204.mail.ne1.yahoo.com>
I agree...axenic amastigotes are more like stressed out promastigotes... I always prefer working with something inside the macrophages as a model for testing drugs or immunomodulators which I believe are closer to actual intracellular amastigotes in the infected host
best
sunil
?
---------------------------------------------------------------
Dr Sunil K.Arora
Professor
In Charge- HIV Diagnostic and Disease Monitoring Laboratory &
NACO State Reference Laboratory,
?
Councillor- Federation of Immunological Societies of Asia-oceania (FIMSA)
Vice President- Indian Immunology Society (IIS)
Secretary (Research)- The Cytometry Society of India (TCS)
?
Department of Immunopathology
PGIMER, Chandigarh-160 012
Ph.: 0091-172-2755192 (Off)
FAX: 0091-172-2744401, 2745078
Ph:+91-172-4666087 (Res); 9872866087 (cell)
email: skarora_in at yahoo.com ; skarorain at gmail.com
________________________________
From: Clos Joachim <clos at bni-hamburg.de>
To: Leish-L email post <Leish-l at lineu.icb.usp.br>
Sent: Monday, 11 March 2013 8:16 PM
Subject: [Leish-l] Fwd: hot spots
Are axenic amastigotes a valid model (and for what?), the first developmental step towards real amastigotes, or just stressed-out promastigotes??Many of us use axenics and swear by them, others are skeptical, others will accept what's inside a macrophage, still others only want amastigotes isolated from something with a fur. I believe we should come to some sort of common language and interpretation. I am somewhere in the middle myself, preferring to do things in macrophages whenever possible.
>Cheers!
>
>
>
>Am 07.02.2013 um 20:38 schrieb Carlos Costa:
>
>What are the most polemic issues in leishmaniasis?
>>
>>
>>--
>>
>>Carlos H. N. Costa, MD, DSc.
>>Sociedade Brasileira de Medicina Tropical?
>>(Brazilian Society of Tropical Medicine)
>>President
>>
>>
>>Universidade Federal do Piau?
>>Instituto de Doen?as Tropicais Natan Portella
>>Rua Artur de Vasconcelos 151-Sul
>>64001-450 Teresina-PIBrazil
>>Telephones: +55 86 3222-4377 (W),?+55 86 3221-3062 (W),?+55 86 3237-1075 (R).?
>>
>>
>>
>>Aviso: As informa??es contidas nesta mensagem s?o CONFIDENCIAIS, protegidas pelo sigilo legal, por direitos autorais e destinadas exclusivamente ? pessoa ou organiza??o para a qual a mensagem foi destinada.
>>Warning: This message is meant only for the intended recipient of the transmission.? It is forbidden any unauthorized use, alteration, reproduction and distribution. If you are not the correct recipient, please notify us immediately by return e-mail and delete this message from your system.
_______________________________________________
>>Leish-l mailing list
>>Leish-l at lineu.icb.usp.br
>>http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l
>>
>
>
>
>Joachim Clos, Dr. rer. nat.
>Senior Staff Scientist
>Group Leader
>Bernhard Nocht Institute for Tropical Medicine
>Bernhard Nocht St. 74, D-20359 Hamburg, FRG
>Phone +49 40 42818-481; Fax -512
>Skype: joachim.clos
>
>
--
This email was sent by icb.usp.br ??????
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From jayusp at hotmail.com Mon Mar 11 18:50:55 2013
From: jayusp at hotmail.com (jeffrey shaw)
Date: Mon, 11 Mar 2013 21:50:55 -0000
Subject: [Leish-l] Remembering Alexandre Peixoto
Message-ID: <DUB124-W26D1B75734B22AB7C606B5CBE10@phx.gbl>
Condolences
for Alexandre Peixoto:
Rhayza
Maingon (d.c.maingon at keele.ac.uk)
I am so, so
sorry, so shocked too. Alex was a great, humane and compassionate researcher
and a good father, husband to his family; a friendly and understanding
colleague when working together in addition to being a brilliant molecular
ecologist and evolutionist. A loss too great to comprehend.
ELCI
VILLEGAS (elciv at ula.ve)
I FEEL SO
MUCH unfortunate loss
Dr. Diwakar
Singh Dinesh (drdsdinesh at yahoo.com)
I feel deep
sorrow to know such sad news. There is great loss in sand fly research. Myself
working in the same field so I am not able to explain my grief in words. May
God give peace to his soul!
Alicia (aiponte at gmail.com)
Very sad
indeed.
More messages can be found on Alexandre`s facebook
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From m.basanez at imperial.ac.uk Mon Mar 11 12:23:46 2013
From: m.basanez at imperial.ac.uk (Basanez, Maria-Gloria)
Date: Mon, 11 Mar 2013 15:23:46 -0000
Subject: [Leish-l] hot spots
In-Reply-To: <5FE4BF79A2094764AF75B5376AB4ADE4@PC01>
References: <CAJsAp4MReYD4uA8q72ULp=HBDGB9WW0mu6rzRJYRE0fga0eC9A@mail.gmail.com>,
<5FE4BF79A2094764AF75B5376AB4ADE4@PC01>
Message-ID: <CB32EBEAD9581E46B18CAF50515551E84791FE43@icexch-m1.ic.ac.uk>
Not sure it's most polemic, but if you are thinking of collating and discussing a research & development agenda for leishmaniasis, I would like to add the updating of mathematical transmission models, including multi-host systems, and the linking of such population dynamic models (rather than static ones) with analyses of economic evaluation for the cost-effectiveness analyses of interventions.
Best wishes to all,
Professor Mar?a-Gloria Bas??ez MSc PhD FRES
Chair in Neglected Tropical Diseases
School of Public Health
Department of Infectious Disease Epidemiology
Faculty of Medicine (St Mary's campus)
Imperial College London
Norfolk Place, London W2 1PG, UK
Tel: + 44 0(20) 75943295
Fax: +44 0(20) 74023927
E-mail: m.basanez at imperial.ac.uk<mailto:m.basanez at imperial.ac.uk>
________________________________
De: leish-l-bounces at lineu.icb.usp.br [mailto:leish-l-bounces at lineu.icb.usp.br] En nombre de Carlos Costa
Enviado el: jueves, 07 de febrero de 2013 8:39
Para: Leish-L
Asunto: [Leish-l] hot spots
What are the most polemic issues in leishmaniasis?
--
Carlos H. N. Costa, MD, DSc.
Sociedade Brasileira de Medicina Tropical
(Brazilian Society of Tropical Medicine)
President
Universidade Federal do Piau?
Instituto de Doen?as Tropicais Natan Portella
Rua Artur de Vasconcelos 151-Sul
64001-450 Teresina-PI
Brazil
Telephones: +55 86 3222-4377 (W),
+55 86 3221-3062 (W),
+55 86 3237-1075 (R).
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From drdsdinesh at yahoo.com Tue Mar 12 08:58:25 2013
From: drdsdinesh at yahoo.com (diwakar dinesh)
Date: Tue, 12 Mar 2013 11:58:25 -0000
Subject: [Leish-l] hot spots
In-Reply-To: <C1C710814C0DA84A9B22DCCBCA8E890E1B9138C9@FDSWP3315.fda.gov>
Message-ID: <1363089500.36975.YahooMailClassic@web142703.mail.bf1.yahoo.com>
Hi,
I do agree with the proposal of Hira and want to add 'Transmission Blocking Vaccine' (TBV)developed with parasite and sand flies to break further transmission of the disease. However, the changing habitat of the vector and their susceptablity status with the insecticide used should be checked simultaneously.
?
With regards
Dinesh
Dr. Diwakar Singh Dinesh
Scientist C (SRO)
Division of Vector Biology and Control
Rajendra Memorial Research Institute of Medical Sciences
(Indian Council of Medical Research)
Department of Health Reseach
Ministry of Health and Family Welfare
Govt. of India
Agamkuan
Patna-800007
--- On Mon, 3/11/13, Nakhasi, Hira <Hira.Nakhasi at fda.hhs.gov> wrote:
From: Nakhasi, Hira <Hira.Nakhasi at fda.hhs.gov>
Subject: Re: [Leish-l] hot spots
To: "leish-l at lineu.icb.usp.br" <leish-l at lineu.icb.usp.br>
Date: Monday, March 11, 2013, 6:13 AM
Carlos,
New Approaches to Vaccine development for Leishmaniasis.
Hira
-----Original Message-----
From: leish-l-bounces at lineu.icb.usp.br [mailto:leish-l-bounces at lineu.icb.usp.br] On Behalf Of Patrick Bastien
Sent: Sunday, February 10, 2013 1:33 PM
To: leish-l at lineu.icb.usp.br
Subject: Re: [Leish-l] hot spots
One example : The existence, modalities and importance of sexuality?
(pr parasexuality).
Cordialement
Professeur Patrick Bastien
Director, Laboratoire de Parasitologie-Mycologie, Faculte de M?decine
Centre National de Reference des Leishmanioses
Deputy Director, UMR MIVEGEC (CNRS 5290 - IRD 224 - Universites Montpellier 1
et 2)
CHU de Montpellier
39 Avenue Charles Flahault
34295 Montpellier Cedex 5, France
----- Message de chncosta at gmail.com ---------
? ???Date?: Thu, 7 Feb 2013 16:38:58 -0300
? ? ? De?: Carlos Costa <chncosta at gmail.com>
R?pondre ??: Carlos Costa <chncosta at gmail.com>
? Objet?: [Leish-l] hot spots
? ? ?????: Leish-L <Leish-l at lineu.icb.usp.br>
> What are the most polemic issues in leishmaniasis?
>
> --
> Carlos H. N. Costa, MD, DSc.
> Sociedade Brasileira de Medicina Tropical
> (Brazilian Society of Tropical Medicine)
> President
>
> Universidade Federal do Piau?
> Instituto de Doen?as Tropicais Natan Portella
> Rua Artur de Vasconcelos 151-Sul
> 64001-450 Teresina-PI
> Brazil
> Telephones: +55 86 3222-4377 (W),
> +55 86 3221-3062 (W),
> +55 86 3237-1075 (R).
>
>
> Aviso: As informa??es contidas nesta mensagem s?o CONFIDENCIAIS, protegidas
> pelo sigilo legal, por direitos autorais e destinadas exclusivamente ?
> pessoa ou organiza??o para a qual a mensagem foi destinada.
> Warning: This message is meant only for the intended recipient of the
> transmission.? It is forbidden any unauthorized use, alteration,
> reproduction and distribution. If you are not the correct recipient, please
> notify us immediately by return e-mail and delete this message from your
> system.
>
----- Fin du message de chncosta at gmail.com -----
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From Albert.Descoteaux at iaf.inrs.ca Fri Mar 29 21:34:18 2013
From: Albert.Descoteaux at iaf.inrs.ca (Descoteaux, Albert)
Date: Fri, 29 Mar 2013 20:34:18 -0400
Subject: [Leish-l] Fwd: hot spots
In-Reply-To: <1363016524.46325.YahooMailNeo@web126204.mail.ne1.yahoo.com>
References: <FE318E54-2932-4C1A-9BAB-D93A0C17F806@bni-hamburg.de>
<89734F2D-D0BF-4B64-ABC4-BFD4BF842160@bni-hamburg.de>
<1363016524.46325.YahooMailNeo@web126204.mail.ne1.yahoo.com>
Message-ID: <01EE24BC-B5A2-4235-BA57-72AE61262B84@iaf.inrs.ca>
Related to this issue, what are really the mammalian signals that induce promastigote-to-amastigote differentiation?
Albert
Envoy? de mon iPad
Le 2013-03-29 ? 15:58, "Sunil Arora" <skarora_in at yahoo.com> a ?crit :
> I agree...axenic amastigotes are more like stressed out promastigotes... I always prefer working with something inside the macrophages as a model for testing drugs or immunomodulators which I believe are closer to actual intracellular amastigotes in the infected host
>
> best
> sunil
>
> ---------------------------------------------------------------
> Dr Sunil K.Arora
> Professor
> In Charge- HIV Diagnostic and Disease Monitoring Laboratory &
> NACO State Reference Laboratory,
>
> Councillor- Federation of Immunological Societies of Asia-oceania (FIMSA)
> Vice President- Indian Immunology Society (IIS)
> Secretary (Research)- The Cytometry Society of India (TCS)
>
> Department of Immunopathology
> PGIMER, Chandigarh-160 012
> Ph.: 0091-172-2755192 (Off)
> FAX: 0091-172-2744401, 2745078
> Ph:+91-172-4666087 (Res); 9872866087 (cell)
> email: skarora_in at yahoo.com ; skarorain at gmail.com
>
> From: Clos Joachim <clos at bni-hamburg.de>
> To: Leish-L email post <Leish-l at lineu.icb.usp.br>
> Sent: Monday, 11 March 2013 8:16 PM
> Subject: [Leish-l] Fwd: hot spots
>
>> Are axenic amastigotes a valid model (and for what?), the first developmental step towards real amastigotes, or just stressed-out promastigotes? Many of us use axenics and swear by them, others are skeptical, others will accept what's inside a macrophage, still others only want amastigotes isolated from something with a fur. I believe we should come to some sort of common language and interpretation. I am somewhere in the middle myself, preferring to do things in macrophages whenever possible.
>> Cheers!
>
>
>>
>>
>> Am 07.02.2013 um 20:38 schrieb Carlos Costa:
>>
>>> What are the most polemic issues in leishmaniasis?
>>>
>>> --
>>> Carlos H. N. Costa, MD, DSc.
>>> Sociedade Brasileira de Medicina Tropical
>>> (Brazilian Society of Tropical Medicine)
>>> President
>>>
>>> Universidade Federal do Piau?
>>> Instituto de Doen?as Tropicais Natan Portella
>>> Rua Artur de Vasconcelos 151-Sul
>>> 64001-450 Teresina-PI
>>> Brazil
>>> Telephones: +55 86 3222-4377 (W), +55 86 3221-3062 (W), +55 86 3237-1075 (R).
>>>
>>>
>>> Aviso: As informa??es contidas nesta mensagem s?o CONFIDENCIAIS, protegidas pelo sigilo legal, por direitos autorais e destinadas exclusivamente ? pessoa ou organiza??o para a qual a mensagem foi destinada.
>>> Warning: This message is meant only for the intended recipient of the transmission. It is forbidden any unauthorized use, alteration, reproduction and distribution. If you are not the correct recipient, please notify us immediately by return e-mail and delete this message from your system.
>>> _______________________________________________
>>> Leish-l mailing list
>>> Leish-l at lineu.icb.usp.br
>>> http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l
>>
>>
>>
>> Joachim Clos, Dr. rer. nat.
>> Senior Staff Scientist
>> Group Leader
>> Bernhard Nocht Institute for Tropical Medicine
>> Bernhard Nocht St. 74, D-20359 Hamburg, FRG
>> Phone +49 40 42818-481; Fax -512
>> Skype: joachim.clos
>
>
>
> --
> This email was sent by icb.usp.br ??
>
> _______________________________________________
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>
>
>
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From olliarop at who.int Fri Mar 29 17:59:12 2013
From: olliarop at who.int (OLLIARO, Piero Luigi)
Date: Fri, 29 Mar 2013 20:59:12 +0000
Subject: [Leish-l] hot spots
In-Reply-To: <20130210193235.78pdjqsgd9j48kc8@webmailum1.univ-montp1.fr>
References: <CAJsAp4MReYD4uA8q72ULp=HBDGB9WW0mu6rzRJYRE0fga0eC9A@mail.gmail.com>
<20130210193235.78pdjqsgd9j48kc8@webmailum1.univ-montp1.fr>
Message-ID: <0E5A42FE07001B43BAAA399A87EDE3C94E1AB0C1@GVA11SSAKURA06.wims.who.int>
I'd definitely second Patrick's proposal
Among other things, (para)sexuality would determine how efficiently drug resistance spreads
Piero Olliaro
-----Original Message-----
From: leish-l-bounces at lineu.icb.usp.br [mailto:leish-l-bounces at lineu.icb.usp.br] On Behalf Of Patrick Bastien
Sent: 10 February 2013 19:33
To: leish-l at lineu.icb.usp.br
Subject: Re: [Leish-l] hot spots
One example : The existence, modalities and importance of sexuality
(pr parasexuality).
Cordialement
Professeur Patrick Bastien
Director, Laboratoire de Parasitologie-Mycologie, Faculte de M?decine
Centre National de Reference des Leishmanioses
Deputy Director, UMR MIVEGEC (CNRS 5290 - IRD 224 - Universites Montpellier 1
et 2)
CHU de Montpellier
39 Avenue Charles Flahault
34295 Montpellier Cedex 5, France
----- Message de chncosta at gmail.com ---------
Date?: Thu, 7 Feb 2013 16:38:58 -0300
De?: Carlos Costa <chncosta at gmail.com>
R?pondre ??: Carlos Costa <chncosta at gmail.com>
Objet?: [Leish-l] hot spots
??: Leish-L <Leish-l at lineu.icb.usp.br>
> What are the most polemic issues in leishmaniasis?
>
> --
> Carlos H. N. Costa, MD, DSc.
> Sociedade Brasileira de Medicina Tropical
> (Brazilian Society of Tropical Medicine)
> President
>
> Universidade Federal do Piau?
> Instituto de Doen?as Tropicais Natan Portella
> Rua Artur de Vasconcelos 151-Sul
> 64001-450 Teresina-PI
> Brazil
> Telephones: +55 86 3222-4377 (W),
> +55 86 3221-3062 (W),
> +55 86 3237-1075 (R).
>
>
> Aviso: As informa??es contidas nesta mensagem s?o CONFIDENCIAIS, protegidas
> pelo sigilo legal, por direitos autorais e destinadas exclusivamente ?
> pessoa ou organiza??o para a qual a mensagem foi destinada.
> Warning: This message is meant only for the intended recipient of the
> transmission. It is forbidden any unauthorized use, alteration,
> reproduction and distribution. If you are not the correct recipient, please
> notify us immediately by return e-mail and delete this message from your
> system.
>
----- Fin du message de chncosta at gmail.com -----
_______________________________________________
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Leish-l at lineu.icb.usp.br
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From skarora_in at yahoo.com Sat Mar 30 01:39:26 2013
From: skarora_in at yahoo.com (Sunil Arora)
Date: Fri, 29 Mar 2013 21:39:26 -0700 (PDT)
Subject: [Leish-l] Fwd: hot spots
In-Reply-To: <51B350881C582C44A8ED53E2FD82FA474C67E48A85@mail02.sbri.org>
References: <FE318E54-2932-4C1A-9BAB-D93A0C17F806@bni-hamburg.de>
<89734F2D-D0BF-4B64-ABC4-BFD4BF842160@bni-hamburg.de>
<1363016524.46325.YahooMailNeo@web126204.mail.ne1.yahoo.com>
<51B350881C582C44A8ED53E2FD82FA474C67E48A85@mail02.sbri.org>
Message-ID: <1364618366.95294.YahooMailNeo@web126202.mail.ne1.yahoo.com>
Ya Peter, I know what you are saying about studying the expression profile of the transcriptome, one has to be careful regarding contaminating RNA from macrophages, in that case yes, one has to depend on axenic amastigotes which are much better purity wise as well as number wise, Thanks
---------------------------------------------------------------
sunil
?
From: Peter Myler <peter.myler at seattlebiomed.org>
To: 'Sunil Arora' <skarora_in at yahoo.com>; Clos Joachim <clos at bni-hamburg.de>; Leish-L email post <Leish-l at lineu.icb.usp.br>
Cc: "danz at bi.technion.ac.il" <danz at bi.technion.ac.il>
Sent: Saturday, 30 March 2013 1:39 AM
Subject: RE: [Leish-l] Fwd: hot spots
Sunil:
?
Axenic amastigotes (Ax) are much more than ?stressed-out promastigotes?.? We (and others) have shown they are very similar (although not identical) to macrophage-derived amastigotes (Am) in terms of global mRNA and protein expression levels.? While it would obviously be better to work with Am, it is often technically difficult due to low numbers and contamination with macrophage RNA and protein.? In addition, it is my contention that the time taken to isolate Am from the macrophages makes them ?stressed-out amastigotes?, which are less physiological relevant than Ax.? We need to approach this discussion from a position of knowledge and data (i.e. understanding exactly which genes are expressed differently in Am and Ax) than belief.? Hopefully, we will be able to publish the results of our RNA-seq studies comparing promastigotes, axenic amastigotes and macrophage-derived amastigotes (without purification) in the not too distant future.
?
Peter ?
?
Peter J. Myler, Ph.D.
Professor
Seattle BioMed
307 Westlake Ave N., Suite 500
Seattle, WA? 98109-5219
USA
(206) 256-7332 (office)
(206) 256-7229 (FAX)
peter.myler at sbri.org
Affiliate?Professor
Departments of Global Health &
Biomedical Informatics and Medical Education
University of Washington
Seattle, WA? 98195
Box 357238
mylerpj at u.washington.edu
?
From:leish-l-bounces at lineu.icb.usp.br [mailto:leish-l-bounces at lineu.icb.usp.br] On Behalf Of Sunil Arora
Sent: Monday, March 11, 2013 8:42 AM
To: Clos Joachim; Leish-L email post
Subject: Re: [Leish-l] Fwd: hot spots
?
I agree...axenic amastigotes are more like stressed out promastigotes... I always prefer working with something inside the macrophages as a model for testing drugs or immunomodulators which I believe are closer to actual intracellular amastigotes in the infected host
?
best
sunil
?
---------------------------------------------------------------
Dr Sunil K.Arora
Professor
In Charge- HIV Diagnostic and Disease Monitoring Laboratory &
NACO State Reference Laboratory,
?
Councillor- Federation of Immunological Societies of Asia-oceania (FIMSA)
Vice President- Indian Immunology Society (IIS)
Secretary (Research)- The Cytometry Society of India (TCS)
?
Department of Immunopathology
PGIMER, Chandigarh-160 012
Ph.: 0091-172-2755192 (Off)
FAX: 0091-172-2744401, 2745078
Ph:+91-172-4666087 (Res); 9872866087 (cell)
email: skarora_in at yahoo.com ; skarorain at gmail.com
?
From:Clos Joachim <clos at bni-hamburg.de>
To: Leish-L email post <Leish-l at lineu.icb.usp.br>
Sent: Monday, 11 March 2013 8:16 PM
Subject: [Leish-l] Fwd: hot spots
?
Are axenic amastigotes a valid model (and for what?), the first developmental step towards real amastigotes, or just stressed-out promastigotes??Many of us use axenics and swear by them, others are skeptical, others will accept what's inside a macrophage, still others only want amastigotes isolated from something with a fur. I believe we should come to some sort of common language and interpretation. I am somewhere in the middle myself, preferring to do things in macrophages whenever possible.
>Cheers!
?
?
Am 07.02.2013 um 20:38 schrieb Carlos Costa:
What are the most polemic issues in leishmaniasis?
?
--
Carlos H. N. Costa, MD, DSc.
Sociedade Brasileira de Medicina Tropical?
(Brazilian Society of Tropical Medicine)
President
?
Universidade Federal do Piau?
Instituto de Doen?as Tropicais Natan Portella
Rua Artur de Vasconcelos 151-Sul
64001-450 Teresina-PI
Brazil
Telephones: +55 86 3222-4377 (W),?
+55 86 3221-3062 (W),?
+55 86 3237-1075 (R).?
?
?
Aviso: As informa??es contidas nesta mensagem s?o CONFIDENCIAIS, protegidas pelo sigilo legal, por direitos autorais e destinadas exclusivamente ? pessoa ou organiza??o para a qual a mensagem foi destinada.
Warning: This message is meant only for the intended recipient of the transmission.? It is forbidden any unauthorized use, alteration, reproduction and distribution. If you are not the correct recipient, please notify us immediately by return e-mail and delete this message from your system.
_______________________________________________
Leish-l mailing list
Leish-l at lineu.icb.usp.br
http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l
?
?
Joachim Clos, Dr. rer. nat.
Senior Staff Scientist
Group Leader
Bernhard Nocht Institute for Tropical Medicine
Bernhard Nocht St. 74, D-20359 Hamburg, FRG
Phone +49 40 42818-481; Fax -512
Skype: joachim.clos
?
--
This email was sent by icb.usp.br ??????
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From peter.myler at seattlebiomed.org Fri Mar 29 17:09:45 2013
From: peter.myler at seattlebiomed.org (Peter Myler)
Date: Fri, 29 Mar 2013 13:09:45 -0700
Subject: [Leish-l] Fwd: hot spots
In-Reply-To: <1363016524.46325.YahooMailNeo@web126204.mail.ne1.yahoo.com>
References: <FE318E54-2932-4C1A-9BAB-D93A0C17F806@bni-hamburg.de>
<89734F2D-D0BF-4B64-ABC4-BFD4BF842160@bni-hamburg.de>
<1363016524.46325.YahooMailNeo@web126204.mail.ne1.yahoo.com>
Message-ID: <51B350881C582C44A8ED53E2FD82FA474C67E48A85@mail02.sbri.org>
Sunil:
Axenic amastigotes (Ax) are much more than "stressed-out promastigotes". We (and others) have shown they are very similar (although not identical) to macrophage-derived amastigotes (Am) in terms of global mRNA and protein expression levels. While it would obviously be better to work with Am, it is often technically difficult due to low numbers and contamination with macrophage RNA and protein. In addition, it is my contention that the time taken to isolate Am from the macrophages makes them "stressed-out amastigotes", which are less physiological relevant than Ax. We need to approach this discussion from a position of knowledge and data (i.e. understanding exactly which genes are expressed differently in Am and Ax) than belief. Hopefully, we will be able to publish the results of our RNA-seq studies comparing promastigotes, axenic amastigotes and macrophage-derived amastigotes (without purification) in the not too distant future.
Peter
Peter J. Myler, Ph.D.
Professor
Seattle BioMed
307 Westlake Ave N., Suite 500
Seattle, WA 98109-5219
USA
(206) 256-7332 (office)
(206) 256-7229 (FAX)
peter.myler at sbri.org<mailto:peter.myler at sbri.org>
Affiliate Professor
Departments of Global Health &
Biomedical Informatics and Medical Education
University of Washington
Seattle, WA 98195
Box 357238
mylerpj at u.washington.edu<mailto:mylerpj at u.washington.edu>
From: leish-l-bounces at lineu.icb.usp.br [mailto:leish-l-bounces at lineu.icb.usp.br] On Behalf Of Sunil Arora
Sent: Monday, March 11, 2013 8:42 AM
To: Clos Joachim; Leish-L email post
Subject: Re: [Leish-l] Fwd: hot spots
I agree...axenic amastigotes are more like stressed out promastigotes... I always prefer working with something inside the macrophages as a model for testing drugs or immunomodulators which I believe are closer to actual intracellular amastigotes in the infected host
best
sunil
---------------------------------------------------------------
Dr Sunil K.Arora
Professor
In Charge- HIV Diagnostic and Disease Monitoring Laboratory &
NACO State Reference Laboratory,
Councillor- Federation of Immunological Societies of Asia-oceania (FIMSA)
Vice President- Indian Immunology Society (IIS)
Secretary (Research)- The Cytometry Society of India (TCS)
Department of Immunopathology
PGIMER, Chandigarh-160 012
Ph.: 0091-172-2755192 (Off)
FAX: 0091-172-2744401, 2745078
Ph:+91-172-4666087 (Res); 9872866087 (cell)
email: skarora_in at yahoo.com<mailto:skarora_in at yahoo.com> ; skarorain at gmail.com<mailto:skarorain at gmail.com>
________________________________
From: Clos Joachim <clos at bni-hamburg.de>
To: Leish-L email post <Leish-l at lineu.icb.usp.br>
Sent: Monday, 11 March 2013 8:16 PM
Subject: [Leish-l] Fwd: hot spots
Are axenic amastigotes a valid model (and for what?), the first developmental step towards real amastigotes, or just stressed-out promastigotes? Many of us use axenics and swear by them, others are skeptical, others will accept what's inside a macrophage, still others only want amastigotes isolated from something with a fur. I believe we should come to some sort of common language and interpretation. I am somewhere in the middle myself, preferring to do things in macrophages whenever possible.
Cheers!
Am 07.02.2013 um 20:38 schrieb Carlos Costa:
What are the most polemic issues in leishmaniasis?
--
Carlos H. N. Costa, MD, DSc.
Sociedade Brasileira de Medicina Tropical
(Brazilian Society of Tropical Medicine)
President
Universidade Federal do Piau?
Instituto de Doen?as Tropicais Natan Portella
Rua Artur de Vasconcelos 151-Sul
64001-450 Teresina-PI
Brazil
Telephones: +55 86 3222-4377 (W),
+55 86 3221-3062 (W),
+55 86 3237-1075 (R).
Aviso: As informa??es contidas nesta mensagem s?o CONFIDENCIAIS, protegidas pelo sigilo legal, por direitos autorais e destinadas exclusivamente ? pessoa ou organiza??o para a qual a mensagem foi destinada.
Warning: This message is meant only for the intended recipient of the transmission. It is forbidden any unauthorized use, alteration, reproduction and distribution. If you are not the correct recipient, please notify us immediately by return e-mail and delete this message from your system.
_______________________________________________
Leish-l mailing list
Leish-l at lineu.icb.usp.br<mailto:Leish-l at lineu.icb.usp.br>
http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l
Joachim Clos, Dr. rer. nat.
Senior Staff Scientist
Group Leader
Bernhard Nocht Institute for Tropical Medicine
Bernhard Nocht St. 74, D-20359 Hamburg, FRG
Phone +49 40 42818-481; Fax -512
Skype: joachim.clos
--
This email was sent by icb.usp.br ??
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From jayusp at hotmail.com Mon Mar 11 18:53:39 2013
From: jayusp at hotmail.com (jeffrey shaw)
Date: Mon, 11 Mar 2013 21:53:39 -0000
Subject: [Leish-l] Remembering Richard Titus
Message-ID: <DUB124-W3598E7B662D02B33094209CBE10@phx.gbl>
Condolences for Richard Titus:
Omar (ohamarsheh at gmail.com)
I am really sorry to hear about Richard,
the sand fly community missed a distinguished scientist. His contribution to
sand fly and Leishmania fields will be printed in minds of all who knew him.
Sergio Mendon?a (mendonca at ioc.fiocruz.br)
This is very sad news.
I had the chance of knowing Rich well
during the more than one year that we worked together in Jacques Louis lab in Lausanne. He was leaving
while I was arriving, and he taught me a lot. Besides being a brilliant
scientist and a hard worker, he was a simple and friendly guy. I feel very sorry for his family and for the
leishmaniac community.
Manoel (mbarralnetto at icloud.com)
Really sad
news. I also knew Richard for a long time and deeply lament his early death.
C1audia (brodskyn at bahia.fiocruz.br)
It is really sad news. I worked at Titus's
lab from 1997 to 2000 and I have wonderful memories from this time. Richard was
great, trying to help us all the time. I learned a lot at his lab and we shared
also good parties and barbecues.
I miss him a lot.
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From Robert.Duncan at fda.hhs.gov Fri Mar 29 17:42:55 2013
From: Robert.Duncan at fda.hhs.gov (Duncan, Robert)
Date: Fri, 29 Mar 2013 20:42:55 +0000
Subject: [Leish-l] Axenic amastigotes
Message-ID: <10CF57B198DB174DA39E61150BB3C57C1FE7522E@FDSWP3313.fda.gov>
I have worked in the lab with axenic L. donovani for 20 years. Evaluating gene expression, cell biology and differentiation. They are an invaluable tool especially for molecular biology which requires a sufficient quantity of material to analyze. However, I consider findings tentative until confirmed in cultured macrophage infections and rodent infections. We have recently begun studies of "full natural cycle transmissions": infected sand flies biting hamsters that develop visceral leishmaniasis, parasites from the infected hamsters fed to sand flies that are used to infect hamsters. Preliminary results suggest that cultured promastigotes are not a perfect model either. We certainly would not give up the years of discovery based on laboratory studies of cultured promastigotes.
-Robert Duncan
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From olliarop at who.int Fri Mar 29 17:49:44 2013
From: olliarop at who.int (OLLIARO, Piero Luigi)
Date: Fri, 29 Mar 2013 20:49:44 +0000
Subject: [Leish-l] hot spots
In-Reply-To: <CB32EBEAD9581E46B18CAF50515551E84791FE43@icexch-m1.ic.ac.uk>
References: <CAJsAp4MReYD4uA8q72ULp=HBDGB9WW0mu6rzRJYRE0fga0eC9A@mail.gmail.com>,
<5FE4BF79A2094764AF75B5376AB4ADE4@PC01>
<CB32EBEAD9581E46B18CAF50515551E84791FE43@icexch-m1.ic.ac.uk>
Message-ID: <0E5A42FE07001B43BAAA399A87EDE3C94E1AB093@GVA11SSAKURA06.wims.who.int>
Mar?a-Gloria :
?Quieres empezar una discusi?n sobre el desarrollo de las drogas?
Then I'd have my own list
But first and foremost: it is becoming VERY difficult today to convince decision makers to fund anti-leishmania drug R&D. So, one would have to start from there: are new drugs needed? and which drugs? and we won't have to preach to the converted (ourselves) but rather to the pagans. Shall we not start from there?
Meanwhile: !Felices pasquas!
Piero
From: leish-l-bounces at lineu.icb.usp.br [mailto:leish-l-bounces at lineu.icb.usp.br] On Behalf Of Basanez, Maria-Gloria
Sent: 11 March 2013 16:24
To: 'Carlos Costa'; 'Leish-L'
Subject: Re: [Leish-l] hot spots
Not sure it's most polemic, but if you are thinking of collating and discussing a research & development agenda for leishmaniasis, I would like to add the updating of mathematical transmission models, including multi-host systems, and the linking of such population dynamic models (rather than static ones) with analyses of economic evaluation for the cost-effectiveness analyses of interventions.
Best wishes to all,
Professor Mar?a-Gloria Bas??ez MSc PhD FRES
Chair in Neglected Tropical Diseases
School of Public Health
Department of Infectious Disease Epidemiology
Faculty of Medicine (St Mary's campus)
Imperial College London
Norfolk Place, London W2 1PG, UK
Tel: + 44 0(20) 75943295
Fax: +44 0(20) 74023927
E-mail: m.basanez at imperial.ac.uk<mailto:m.basanez at imperial.ac.uk>
________________________________
De: leish-l-bounces at lineu.icb.usp.br<mailto:leish-l-bounces at lineu.icb.usp.br> [mailto:leish-l-bounces at lineu.icb.usp.br] En nombre de Carlos Costa
Enviado el: jueves, 07 de febrero de 2013 8:39
Para: Leish-L
Asunto: [Leish-l] hot spots
What are the most polemic issues in leishmaniasis?
--
Carlos H. N. Costa, MD, DSc.
Sociedade Brasileira de Medicina Tropical
(Brazilian Society of Tropical Medicine)
President
Universidade Federal do Piau?
Instituto de Doen?as Tropicais Natan Portella
Rua Artur de Vasconcelos 151-Sul
64001-450 Teresina-PI
Brazil
Telephones: +55 86 3222-4377 (W),
+55 86 3221-3062 (W),
+55 86 3237-1075 (R).
Aviso: As informa??es contidas nesta mensagem s?o CONFIDENCIAIS, protegidas pelo sigilo legal, por direitos autorais e destinadas exclusivamente ? pessoa ou organiza??o para a qual a mensagem foi destinada.
Warning: This message is meant only for the intended recipient of the transmission. It is forbidden any unauthorized use, alteration, reproduction and distribution. If you are not the correct recipient, please notify us immediately by return e-mail and delete this message from your system.
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From danz at bi.technion.ac.il Sat Mar 30 12:30:51 2013
From: danz at bi.technion.ac.il (danz at bi.technion.ac.il)
Date: Sat, 30 Mar 2013 15:30:51 +0000
Subject: [Leish-l] Fwd: hot spots
In-Reply-To: <1363016524.46325.YahooMailNeo@web126204.mail.ne1.yahoo.com>
References: <FE318E54-2932-4C1A-9BAB-D93A0C17F806@bni-hamburg.de>
<89734F2D-D0BF-4B64-ABC4-BFD4BF842160@bni-hamburg.de>
<1363016524.46325.YahooMailNeo@web126204.mail.ne1.yahoo.com>
Message-ID: <66EABADC-8779-449B-8F56-BE3F45C9B93F@cc.technion.ac.il>
Hi Sunil,
May I add to Peter's remarks. We and others showed that axenic differentiation initiates only when promastigotes expose to both high temperature and acidic pH. If stress was the signal, temperature or pH alone could induce differentiation. One important feature of the host-free or axenic system is that we can perform time course analysis of the process of differentiation, thereby evaluate/reveal pathways responsible for Leishmania development. This is something you cannot do neither in macrophages nor in animal models. In vivo validation can follow experiment in axenic system. Finally, in papers that we have published to date, some of which were done in collaboration with Peter, we demonstrated that signal-induced differentiation in axenic conditions is highly regulated process and reasonable representation of intracellular amastigotes. We will soon publish a paper that for the first time reveals the signaling pathway that initiates promastigote to amastigote differentiation. My graduate student will reveal this pathway in her talk at WL5.
The axenic system is the best in vitro system that simulate differentiation. Finally, do not forget that the culture promastigotes all of us use are axenic promastigotes...
See you all in May
Dan
Professor Dan Zilberstein, Executive director
Center for Pre Academic Education
And The Faculty of Biology
Technion-Israel Institute of Technology
Haifa 32000, Israel
?-29 ???? 2013, ???? 16:09, "Sunil Arora" <skarora_in at yahoo.com<mailto:skarora_in at yahoo.com>> ???/?:
I agree...axenic amastigotes are more like stressed out promastigotes... I always prefer working with something inside the macrophages as a model for testing drugs or immunomodulators which I believe are closer to actual intracellular amastigotes in the infected host
best
sunil
---------------------------------------------------------------
Dr Sunil K.Arora
Professor
In Charge- HIV Diagnostic and Disease Monitoring Laboratory &
NACO State Reference Laboratory,
Councillor- Federation of Immunological Societies of Asia-oceania (FIMSA)
Vice President- Indian Immunology Society (IIS)
Secretary (Research)- The Cytometry Society of India (TCS)
Department of Immunopathology
PGIMER, Chandigarh-160 012
Ph.: 0091-172-2755192 (Off)
FAX: 0091-172-2744401, 2745078
Ph:+91-172-4666087 (Res); 9872866087 (cell)
email: skarora_in at yahoo.com<mailto:skarora_in at yahoo.com> ; skarorain at gmail.com<mailto:skarorain at gmail.com>
________________________________
From: Clos Joachim <clos at bni-hamburg.de<mailto:clos at bni-hamburg.de>>
To: Leish-L email post <Leish-l at lineu.icb.usp.br<mailto:Leish-l at lineu.icb.usp.br>>
Sent: Monday, 11 March 2013 8:16 PM
Subject: [Leish-l] Fwd: hot spots
Are axenic amastigotes a valid model (and for what?), the first developmental step towards real amastigotes, or just stressed-out promastigotes? Many of us use axenics and swear by them, others are skeptical, others will accept what's inside a macrophage, still others only want amastigotes isolated from something with a fur. I believe we should come to some sort of common language and interpretation. I am somewhere in the middle myself, preferring to do things in macrophages whenever possible.
Cheers!
Am 07.02.2013 um 20:38 schrieb Carlos Costa:
What are the most polemic issues in leishmaniasis?
--
Carlos H. N. Costa, MD, DSc.
Sociedade Brasileira de Medicina Tropical
(Brazilian Society of Tropical Medicine)
President
Universidade Federal do Piau?
Instituto de Doen?as Tropicais Natan Portella
Rua Artur de Vasconcelos 151-Sul
64001-450 Teresina-PI
Brazil
Telephones: +55 86 3222-4377 (W),
+55 86 3221-3062 (W),
+55 86 3237-1075 (R).
Aviso: As informa??es contidas nesta mensagem s?o CONFIDENCIAIS, protegidas pelo sigilo legal, por direitos autorais e destinadas exclusivamente ? pessoa ou organiza??o para a qual a mensagem foi destinada.
Warning: This message is meant only for the intended recipient of the transmission. It is forbidden any unauthorized use, alteration, reproduction and distribution. If you are not the correct recipient, please notify us immediately by return e-mail and delete this message from your system.
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Joachim Clos, Dr. rer. nat.
Senior Staff Scientist
Group Leader
Bernhard Nocht Institute for Tropical Medicine
Bernhard Nocht St. 74, D-20359 Hamburg, FRG
Phone +49 40 42818-481; Fax -512
Skype: joachim.clos
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From jayusp at hotmail.com Fri Mar 29 16:36:23 2013
From: jayusp at hotmail.com (jeffrey shaw)
Date: Fri, 29 Mar 2013 19:36:23 +0000
Subject: [Leish-l] FW: PRO/AH/EDR> Leishmaniasis - Syria (02)
In-Reply-To: <CAChMjg8ZJF17QXvzDEhWnfRF+vgvx=wFQgDbhBjem9Z0nDQPfQ@mail.gmail.com>
References: <201303290003.UAA24953@promed.harvard.edu>,
<CAChMjg8ZJF17QXvzDEhWnfRF+vgvx=wFQgDbhBjem9Z0nDQPfQ@mail.gmail.com>
Message-ID: <DUB124-W20A1C450BAC1E250376E3ACBD30@phx.gbl>
---------- Forwarded message ----------
From: ProMED-mail <promed at promed.isid.harvard.edu>
Date: 2013/3/28
Subject: PRO/AH/EDR> Leishmaniasis - Syria (02)
To: promed-ahead-edr at promedmail.org
LEISHMANIASIS - SYRIA (02)
**************************
A ProMED-mail post
<http://www.promedmail.org>
ProMED-mail is a program of the
International Society for Infectious Diseases
<http://www.isid.org>
Date: Thu 28 Mar 2013
Source: Today's Zaman [edited]
<http://www.todayszaman.com/news-310925-leishmaniasis-outbreak-rings-alarm-bells-at-turkish-syrian-border.html>
Approximately 100 000 people have been infected with the leishmaniasis
parasitic disease in the past 2 years after civil war broke out in
Syria, compared with before the conflict when the number of cases in
Syria had been reduced to 3000-4000 as a result of joint efforts by
Turkish and Syrian authorities.
The increase in the number of patients suffering from the disease is
alarming not only for Syria but for Turkey as well, since
leishmaniasis has also reached Turkey.
Opposition forces who have been leading an uprising against Syrian
President Bashar al-Assad's rule have asked Turkey to deliver 10 000
boxes of Glucantin [supposedly the meglumine antimoniate Glucantime],
a medicine used to treat leishmaniasis, which amounts to one month's
treatment of 20 000 people.
However, there has been no positive response from the Turkish side
yet. Fake Glucantin is being produced and sold for 50 percent less
than the normal price for the medicine in Aleppo.
Dr. Kerem Kinik, coordinator of the Association of Earth Doctors, told
Today's Zaman that his organization has been working to help the
Syrians fight the disease. "We'll provide assistance to fight the
disease, starting with sending medicine. This step is also important
to protect our country [Turkey] from the disease," he said.
The number of doctors in Syria has dropped dramatically from 30 000 to
5000, and 57 percent of the hospitals have been damaged in the
conflict, according to Kinik.
Before the civil war in Syria, the number of leshmaniasis patients had
been reduced to a great extent after studies carried out in 45 centers
in 14 provinces across the nation. After a Syrian health official fled
to Canada following the start of the conflict in the country, the
battle against the disease was halted.
Experts argue that the nation also faces typhoid, cholera and
tuberculosis epidemics.
Turkey imports leshmaniasis medicine via the Turkish Pharmacists'
Association (TEB). TEB Secretary General Harun Kizilay told Today's
Zaman that Turkey could bring the drugs to combat the disease for
Syrians should the Turkish Prime Ministry's Disaster and Emergency
Management Directorate (AFAD) submit a request at the Health
Ministry.
Professor Fatih Koksal, from Cukurova University's department of
microbiology and clinical microbiology, said _Leishmania infantum_, a
parasite that causes leshmaniasis, has been more common in Turkey in
the recent years. "There was one case each in 2005 and 2010. But now,
30 out of every 100 cases are caused by this parasite. Measures need
to be taken," he urged.
Koksal said he visited the buffer zone between the Cilvegozu and Bab
al-Hawa border gates and observed poor conditions there that might
lead to other epidemics. "The passage of people [from Syria] to Turkey
has increased the frequency of diseases in Turkey."
The symptoms of leshmaniasis are skin sores, fever and anemia and may
result in spleen and liver problems. The disease can be fatal if not
treated. The disease is transmitted by certain species of sandfly and
later passes on to humans from animals, including dogs.
[Byline: Caglar Avci]
--
Communicated by:
ProMED-mail <promed at promedmail.org>
[This is probably cutaneous leishmaniasis, which is the most common
form of leishmaniasis in Syria.
It is difficult to confirm the numbers, but as ProMED commented in our
26 Dec 2012 posting on leishmaniasis in Syria, the infection will
increase. It will be interesting to get numbers of cases in Syrian
refugees in Turkey, Lebanon and Jordan. - Mod.EP
A HealthMap/ProMED-mail map can be accessed at:
<http://healthmap.org/r/3aLZ>.]
[see also:
Leishmaniasis - Syria: RFI 20130227.1562934
2012
----
Leishmaniasis, tuberculosis - Syria (02): comment 20121227.1471041
Leishmaniasis, tuberculosis - Syria 20121226.1470184]
.................................................sb/ep/msp/dk
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