[Leish-l] inquiry

Thu Jun 2 15:10:26 BRT 2011

Dear Dr. Sharma,

Is the L donovani in the Satluj River Valley different from the L donovani in Bihar ? This is an important point.

The genetic site we examined did reveal identity of your samples with L infantum, but differs from L donovani from Bihar and Sudan. Have you not found rK39+ dogs ?  In one of your samples, we saw evidence of both L infantum and L tropica. Whether this is a hybrid or a mixture of the two cannot be certain.

It would be good if Petr Volf could have an opportunity of looking into the sand flies there ? 

KP

________________________________

From: Sharman [mailto:nandlals at hotmail.com] 
Sent: Wednesday, June 01, 2011 11:40 AM
To: Raj; Petr Volf; Chang, Kwang-Poo; leish-l at lineu.icb.usp.br; hgoto at usp.br; elfadil_abass at yahoo.com; vishwamohan_katoch at yahoo.co.in
Subject: Re: [Leish-l] inquiry

Dear all,

The discussion is getting very interesting. To let you know our focus of CL in India, I.e. Satluj Valley focus has got L donovani predominantly and L . tropica as well. Two Labs conformed L donovani with approximately 4: 1 ratio  to L. tropica. Some more samples  tested by Dr. Chang revealed L infantum and L tropica. Results of First study were reported in AJTMH in 2005. L.infantum results still unpublished. Another peculiar observation we observed in a child patient was that after injecting SSG intralesionally white spots developed at the injection sites. Whether this was toxicity of SSG or it is a counterpart of PKDL as L. donovani is the cause is a question still to be answered. As I mentioned earlier some of the patients' lesional exudate was also rK 39 positive. Well number of questions unanswered. Will try to find answers.

NLS 

From: Raj <mailto:raj at cellabs.com.au>  

Sent: Wednesday, June 01, 2011 8:07 AM

To: Petr Volf <mailto:volf at cesnet.cz>  ; Chang, Kwang-Poo <mailto:Kwang-Poo.Chang at rosalindfranklin.edu>  ; leish-l at lineu.icb.usp.br ; nandlals at hotmail.com ; hgoto at usp.br ; elfadil_abass at yahoo.com 

Subject: Re: [Leish-l] inquiry

 G'Day Dr Petr Volf,

Thank you very much for your paper. Excellent molecular typing of parasites. 

As you see my correspondence below, we have a antibody ELISA where we use exoantigens from individual L. infantum and L. tropica. 

 If you are interested I can test plasma/serum samples for presence of antibody response. If so  please let me know.

Raj

Cellabs

G'Day Dr Hanafi,

My enquiry was to know whether L. donovani in India produces a cutaneous form of lesion or not (I am not referring to PKDL form). There are many reports from SriLanka about the cutaneous manifestations by L. donovani. The PKDL although is a  cutaneous response appears very late in the infection process and this is ascribed to be a protective response against the parasite. 

Prof Chang mentioned 

"Analyses of several batches of basically clinical CL samples from Dr. Sharma showed evidence of L infantum, but also L tropica as well as a mixture of the two in one sample. This is the same picture we have noted for samples from Hatay, Turkey." This is from Sutlej region." It means this cutaneous  complex requires some investigation. 

I raise species-specific exo-antigens from L. tropica and L infantum and I am prepared to test the serum /plasma samples for presence of antibodies.  If Dr NL Sharma is prepared to send me specimens as well as clinical plasma or serum samples I would be interested to investigate the antibody response in these situations. 

Dr Raj

Cellabs

	----- Original Message ----- 

	From: Petr Volf <mailto:volf at cesnet.cz>  

	To: Raj <mailto:raj at cellabs.com.au>  

	Sent: Tuesday, May 31, 2011 5:08 PM

	Subject: Fw: [Leish-l] inquiry

	Dear Dr Raj,

	I saw your question to Dr. Sharma. I am forwarding my response to him as well the paper which might be interesting also for you.

	Best wishes

	Petr Volf

	----- Original Message ----- 

	From: Petr Volf <mailto:volf at cesnet.cz>  

	To: Chang, Kwang-Poo <mailto:KwangPoo.Chang at rosalindfranklin.edu>  ; Sharman <mailto:nandlals at hotmail.com>  ; Hiro Goto <mailto:hgoto at usp.br>  ; elfadil abass <mailto:elfadil_abass at yahoo.com>  

	Cc: leish-l at lineu.icb.usp.br 

	Sent: Tuesday, May 31, 2011 9:06 AM

	Subject: Re: [Leish-l] inquiry

	Hi K.P.,

	in Cukurova region, Turkey, cutaneous L. infantum (now it seems that we are dealing with L.donovani/L. infantum hybrid) grew very poorely if we isolated them from patients. Only 1 of 25 isolations was succesfull. However, the same strain (confirmed by molecular methods) grew repeatedly and very well if we isolated them from sandflies. It might be useful for Dr. Sharma try to get isolates from sand flies. It is very laborious but very useful, in Cukurova we got about dozen of isolates by this method (all identical).

	Patients are rK39 negative, see attached paper.

	Best wishes

	Petr

		----- Original Message ----- 

		From: Chang, Kwang-Poo <mailto:KwangPoo.Chang at rosalindfranklin.edu>  

		To: Sharman <mailto:nandlals at hotmail.com>  ; Hiro Goto <mailto:hgoto at usp.br>  ; elfadil abass <mailto:elfadil_abass at yahoo.com>  

		Cc: leish-l at lineu.icb.usp.br 

		Sent: Sunday, May 22, 2011 1:39 AM

		Subject: Re: [Leish-l] inquiry

		Dr. NL Sharma has been working on an important CL endemic area along the Satluj River valley to the south of Himalaya in India (Please correct me should I be wrong for anything I said here and below). I had the good fortune of visiting the site several years back courtesy of Dr. Sharma. 

		I believe Dr. Sharma's finding is important, since the parasites there are different from the familiar Indian L donovani in Bihar. The parasites are refratory to in vitro cultivation. They do differentiate into promastigotes and may grow a little, but  can't really be subcultured to establish passageable lines. This is very much reminescent of L infantum in the Mediterranean area. I recall Dr. Sharma has also found rK39+ dogs, if I remember correctly. If so,  Satluj river valley is endemic to the infantile CL.

		Analyses of several batches of basically clinical CL samples from Dr. Sharma showed evidence of L infantum, but also L tropica as well as a mixture of the two in one sample. This is the same picture we have noted for samples from Hatay, Turkey.

		These observations make me wonder a lot about our current knowledge on the clinico-epidemiology based on data collected previously by analyses of cultured promastigotes from one or few 'representative samples'. Nowaday, technology makes it very doable to work with biological samples for Leish DNAs directly from sand flies, patients and reservoir animals.

		KP

________________________________

		From: leish-l-bounces at lineu.icb.usp.br on behalf of Sharman
		Sent: Fri 5/20/2011 11:31 PM
		To: Hiro Goto; elfadil abass
		Cc: leish-l at lineu.icb.usp.br
		Subject: Re: [Leish-l] inquiry

		Dear all
		I agree with Hiro gito, We are working on a focus where the CL is
		predominantly caused by L. donovani, and the rK 39 STRIPS GIVE POSITIVE
		RESULTS WITH SERA as well as serous exudate from the lesion. The results are
		dependent upon species.
		NL Sharma

		--------------------------------------------------
		From: "Hiro Goto" <hgoto at usp.br>
		Sent: Wednesday, May 18, 2011 12:09 AM
		To: "elfadil abass" <elfadil_abass at yahoo.com>
		Cc: <leish-l at lineu.icb.usp.br>
		Subject: Re: [Leish-l] inquiry

		> Dear all,
		> In our oppinion, DAT and rK39 for those samples are not indicated  since
		> these tests are produced for the diagnosis of visceral  leishmaniasis.
		> In case of tegumentary leishmaniasis, it is very appropriate the
		> observation of J.J. Shaw appointing species specificity of antibody
		> response in these cases. Titers of antibodies are in general low in  these
		> cases therefore depending on the species, it may result  negative. We have
		> published a review recently in Expert Rev. Anti  Infect. Ther. 8(4),
		> 419?433 (2010), Current diagnosis and treatment of  cutaneous and
		> mucocutaneous leishmaniasis, where we raise this point.
		> Hiro Goto
		>
		> Citando elfadil abass <elfadil_abass at yahoo.com>:
		>
		>> Dear all I would recommend using DAT and rK39 strip test to measure
		>> antibody
		>> responses and to evaluate the diagnostic efficiency for both tests  in
		>> such group
		>> of patients.
		>>
		>>  Elfadil Abass
		>> ________________________________________________________________________________
		>> Institute of Medical Microbiology and Hospital Hygiene
		>> Philipps University Marburg
		>> BMFZ / Hans-Meerwein Straße 2
		>> D-35033 Marburg, Germany
		>> ________________________________________________________________________________
		>>
		>>
		>>
		>>
		>> ________________________________
		>> From: Nuha Nuwayri-Salti <racha at aub.edu.lb>
		>> To: saad saad <saad1426 at gmail.com>; "leish-l at lineu.icb.usp.br"
		>> <leish-l at lineu.icb.usp.br>
		>> Sent: Fri, May 13, 2011 9:39:33 AM
		>> Subject: Re: [Leish-l] inquiry
		>>
		>> Dear Saad first precaution to take is to separate your samples into
		>> several
		>> portions each (at least 5 each being no more than a few hundred(200-400)
		>> microliters). This is a necessary precaution to avoid freezing and
		>> thawing
		>> several times the same sample which will be the case should you do
		>> different
		>> studies at different times which is unavoidable.
		>>
		>> What you can do is correlate the type(ulcerated, abscess, furuncle
		>>  etc.. ) the
		>> locale, the number and age of lesions with the levels of antibody in  the
		>> sera of
		>> these patgients and also monitor cell mediated immunity with leishmanin
		>> skin
		>> test!
		>> I have just published (in print) an article about having circulating
		>> parasites
		>> in some of these patients with apparently pure cutaneous disease. It is
		>> the
		>> first paper that revealed this fact. It would be interesting to confirm
		>> or
		>> de-confirm this fact repeating what we did.
		>> Best wishes
		>>
		>> Nuha Nuwayri-Salti MD
		>>
		>>
		>>
		>> AOA
		>> Medical Honor Society
		>>
		>>
		>>
		>>
		>> -----Original Message-----
		>> From: leish-l-bounces at lineu.icb.usp.br
		>> [mailto:leish-l-bounces at lineu.icb.usp.br]
		>> On Behalf Of saad saad
		>> Sent: Friday, April 29, 2011 11:23 PM
		>> To: leish-l at lineu.icb.usp.br
		>> Subject: [Leish-l] inquiry
		>>
		>> Dear all
		>> Hi. i am Saad from Saudi Arabia. i have 60 sera from positive case of
		>> CL from south west of the country. I would like to have your
		>> recommendations to start a good research line in CL using these sera.
		>> Thanks in advance for your help
		>> _______________________________________________
		>> Leish-l mailing list
		>> Leish-l at lineu.icb.usp.br
		>> http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l
		>> _______________________________________________
		>> Leish-l mailing list
		>> Leish-l at lineu.icb.usp.br
		>> http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l
		>>
		>
		>
		>
		> Profa. Dra. Hiro Goto
		> Laboratório de Soroepidemiologia e Imunobiologia
		> Instituto de Medicina Tropical de São Paulo, USP
		> Av. Dr. Enéas de Carvalho Aguiar, 470, prédio II, quarto andar
		> 05403-000 - São Paulo, SP
		> Tel. +55-11-3061 7023, 3061 7056 ou 3061 7027
		> Fax. +55-11-3061-8270
		>
		> _______________________________________________
		> Leish-l mailing list
		> Leish-l at lineu.icb.usp.br
		> http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l
		>
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